Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,980,226 G→A V91I (GTT→ATT)  rffM → UDP‑N‑acetyl‑D‑mannosaminuronic acid transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,980,2260GA100.0% 50.0 / NA 17V91I (GTT→ATT) rffMUDP‑N‑acetyl‑D‑mannosaminuronic acid transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/8);  total (9/8)

CGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTCAGGGAGTTAATTAACGCTGCCGAATTTAAATATGCGGATGGCATCAGCGTTGTACGTTCAGTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGACGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTGCGCAACCAGTGGAATGTGAATATCGTTGGCAGTCAGGATGGTTATTTTAAACCCGAGCAGCGTCAGGCGCTGTTTGAACGCATTCATGCCAGCGGTGCGCAAATCGTCACCGTTGCGATGGGATCGCC  >  NC_000913/3979987‑3980467
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                 
cGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCGTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTCAGGGAGTTAATTAACGCTGCCGAATTTAAATATGCGGATGGCATCAGCGTTGTACGTTCAGTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCATTGCCGGTGcc                                                                                                                                                                                                                                        <  1:204809/251‑1 (MQ=255)
                gTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATATGTTTGCTGCCGGGCCGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGCAAAAATGCTGACTATTGAAGATAACGCCGAGGTCAGGGAGTTAATTAACGCTGCCGAATTTAAATATGCGGATGGCATCAGCGTTGTACGTTCAGTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCATTGCCGGTGCCGATCTCTGGGCAGAGc                                                                                                                                                                                                                        >  1:49047/1‑251 (MQ=255)
                                             cccTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGACCGCTGGTTGCCATTAATGCTGAAAAAATGCTGCCTATTGAAGATACCGCCGAGGTCAGGGAGTTAATTAACGCTGCCGAATTTAAATATGCGGATGGCATCAGCGTTGTACGTTCAGTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCAGCATTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCGGGCAAAGAAGGGACg                                                                                                                                                                                           >  1:250684/1‑251 (MQ=255)
                                                                                  gggAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATACCGCCGAGGTCAGGGAGTTAATTAACGCTGCCGAATTTAAATATGCGGATGGCATCAGCGTTGTACGTTCAGTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCATTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGACGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTgg                                                                                                                                                      >  1:259792/1‑251 (MQ=255)
                                                                                                                                          aGGTCAGGGAGTTAAGTAACGCTGCCGAATTTAAATATGCGGATGGCATCAGCGTTGTACGTTCAGTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCATTGCCGGTGCCGTTCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGACGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTGCGCAACCAGTGGAATGTGAATATCGTTGGCAGTCAg                                                                                              <  1:125759/251‑1 (MQ=255)
                                                                                                                                                gggAGTTAATTAACGCTGCCGAATTTAAATATGCGGATGGCATCAGCGTTGTACGTTCAGTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCATTGCCGGTGCCGATCTCTGGGAAGAGCTGATgn                                                                                                                                                                                                                  <  1:34503/129‑2 (MQ=255)
                                                                                                                                                           aaCGCTGCCGAATTTAAATATGCGGATGGCATCAGCGTTGTACGTTCAGTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCATTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGACGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTGCGCAACCAGTGGAATGTGAATATCGTTGGCAGTCAGGATGGTTATTTTAAAcc                                                                             <  1:79969/251‑1 (MQ=255)
                                                                                                                                                                 gCCGAATTTAAATATGCGGATGGCATCAGCGTTGTACGTTCAGTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCATTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAGGGGACGCCGGTATTTCTTGGGGGCGGTAAACCTGAAGTTCTGGCGGAAACTGAAGCTAAACTGCGCAACCAGTGGAATGTGAATATCGTTGGCAGTCAGGATGGTTATTTTAAACCCGagc                                                                        <  1:116334/250‑1 (MQ=255)
                                                                                                                                                                                    aTGGCATCAGCGTTGTACGTTCAGTACGTAAAAAGTACCCGCAGGCGCAAGTCTCCCGCATTGCAGGTGCCGCACTCTGGGCAGAGCTGATGGCCCACGCAAGCCAAGACGGGACGCCGGTCTTTCTTATGGGCGGTAAACCTGAAGTGATGGCGCAAACTGCAGCTAACCTGCGCACCCAGCGGAATGCGAAAATCGTTGGCAGTAAGGATGGTTATTTTAAAc                                                                              >  1:145213/1‑225 (MQ=255)
                                                                                                                                                                                              cGTTGTACGTTCAGTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCATTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGACGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGgc                                                                                                                                                     >  1:157104/1‑144 (MQ=255)
                                                                                                                                                                                                           gTACGTANAAANTACCNGCNGGCGCAGGTTTCCCGNATTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGNNANNNANGGACGCCg                                                                                                                                                                                        <  2:198897/96‑1 (MQ=255)
                                                                                                                                                                                                           gTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCATTGCCGGTGCCGATCTCTGGGAAGAGATGATGGCGCGCGCAGGCAAAGAAGGGACGCCg                                                                                                                                                                                        >  1:198897/1‑96 (MQ=255)
                                                                                                                                                                                                                   aaaGTACCCGCAGGCGCAGGTTTCCCGCATTGCCGGTGCCGATCTCTGGGGAGAGCTGATGGCGCGCGCAGGCAACGAAGGGACGCCGGTATTTCTTGTGGGCGGTAAACCTGAAg                                                                                                                                                            >  1:213591/1‑116 (MQ=255)
                                                                                                                                                                                                                   aaaGTACCCGCAGGCGCAGGTNTCCCGCATTGNCGGNNCCGANCNCNGGGAAGAGCTGATGGCGCGCGCAGGCAAANAAGGGACGCCGGTATTTCTTGTGNNCGNNNAANNTGAAg                                                                                                                                                            <  2:213591/116‑1 (MQ=255)
                                                                                                                                                                                                                         ccagcnngcnnnggNNTCCCGCATTGCCGGTGCCGATCTNTGGGAAGAGATGATGgcg                                                                                                                                                                                                                >  2:211710/4‑58 (MQ=255)
                                                                                                                                                                                                                         ccagcaggcgcaggTTTCCCGCATTGCCGGTGCCGATCTCTGGGAAGAGCTGATGgcg                                                                                                                                                                                                                <  1:211710/55‑1 (MQ=255)
                                                                                                                                                                                                                                      gTTTCCCGCATTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGACGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTGCGCAACCAGTGGAATGTGAATATCGTTGGCAGTCAGGATGGTTATTTTAAACCCGAGCAGCGTCAGGCGCTGTTTGAACGCATTCATGCCAGCGGTGCGCAAATCGTCACCGTTGCGATGGGATCGcc  >  1:114495/1‑251 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                 
CGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTCAGGGAGTTAATTAACGCTGCCGAATTTAAATATGCGGATGGCATCAGCGTTGTACGTTCAGTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGACGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTGCGCAACCAGTGGAATGTGAATATCGTTGGCAGTCAGGATGGTTATTTTAAACCCGAGCAGCGTCAGGCGCTGTTTGAACGCATTCATGCCAGCGGTGCGCAAATCGTCACCGTTGCGATGGGATCGCC  >  NC_000913/3979987‑3980467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑