| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,908,319 | C→G | D450H (GAC→CAC) | pyrG ← | CTP synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,908,319 | 0 | C | G | 84.6% | 16.9 / ‑5.0 | 13 | D450H (GAC→CAC) | pyrG | CTP synthetase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (4/7); minor base T (0/2); total (4/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.39e-01 | |||||||||||
AAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTCAACCAACTGGCACTGCTGTGCGCCGAGACGCATGGTACCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCG > NC_000913/2908072‑2908500 | aaGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTgaac < 1:169249/251‑1 (MQ=255) cATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGGAATTAGGAACTTCGATGATCTCGACCAACTGATCATCCCAGGAACAGCCCGCAACGCGCAGACATGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGAAGCTCAACAATTCTCGGCGCATTGTACAGCTGGAGAACAAGGCTATCGTGAACCAACTGGCACTGCTGTGCGCCg > 1:139207/1‑251 (MQ=255) aGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGGTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTGAACCAACTGGCACTGCTGTGCGCCGAGACGCATGGTAccgc < 1:228728/251‑1 (MQ=255) cTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTGAACCAACTGGCACTGCTGTGCGCCGAGACGCATGGTACCGCCGAGATCGCTCTTCTCGc > 1:5656/1‑251 (MQ=255) cccatgggTGATTCGGAACTTCGATGATCTCGACCAACTGTTCATCCCCGGAACGGCCCTCAACGCGCAGCCCTCCTTCTTCAATCTTTTTCAACAGCTTTTTTTTGACTTCGTCACGGTGCCGTTTCCGCTCACCAATTTCCGGCGCATTCCCCATCTGGCGAACCCGGCTATCGTGAACCATCTGGCCCTGCTGTGTGCCGATCCGCATGGTTCCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACg < 1:277807/246‑1 (MQ=255) aTTCGGAACTTCGATGATCTCGACCAACTGATCAGCCCCGGAACGGCCCGCACCGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGGCGGCGCATTGTACAGCTGGCTAACCAGCCTATCGTGAACCAACTGGCACTGCTGTGCGCCGAGACGCAGGGTACCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTgn < 1:50436/245‑2 (MQ=255) gcGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTTTTTCAACAGCATGTTTTTGACTTCGTAACGGTGACGAGGACTCTCAACAATGGTCGGGGCATTGTGCATCGGGCGAACCAGGCTATCGTTAACCAACTGGCACTTCTGTGCTCCGGGACGCATGGTACCGCCGAGATCGCTCTTGTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGcc < 1:133684/249‑1 (MQ=255) natgatCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTGAACCAACTGGCACTGCTGTGCGCCGAGACGCATGGTACCGCCGAGATCGc > 1:25367/2‑206 (MQ=255) aCGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGCCTATCGTGAACCAACAGGCACTTCTGTGCGCCGAGACGCATTTGACCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAgcg < 1:189828/233‑1 (MQ=255) tCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTTAACCAACTGGCACTGCGGTGCGCCGAGACGCATGGTACCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTgg < 1:254022/250‑1 (MQ=255) gTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTGAACCAACTGTCACTGCTGTGCGCCGAGACGCAAGGTACCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGTTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTtctc < 1:283558/250‑1 (MQ=255) nttCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTGAACCAACTGGCACTGCTGTGCGCCGAGACGCATGGTACCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCg > 1:182326/2‑251 (MQ=255) cGCTCAACAATTGTCGGCGCATTGTACAGCTGTCGAACCAGGCTATCTTGAACCAACTGGCACTGCTGTGCGCCGAGACGCATGGt < 1:271339/86‑1 (MQ=255) tNNACAGCTNNCGAACCAGGCTATCGTGAACAAACTGGCACTGCTGTGCGCCGGGACGCATGGTACCGCCGAGATCGCTCTTCTCGCTACGAACTTCaa < 2:5656/99‑1 (MQ=255) | AAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTCAACCAACTGGCACTGCTGTGCGCCGAGACGCATGGTACCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCG > NC_000913/2908072‑2908500 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |