| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,032,423 | G→A | V219V (GTC→GTT) | yedJ ← | putative HD superfamily phosphohydrolase YedJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,032,423 | 0 | G | A | 92.9% | 31.2 / ‑6.1 | 14 | V219V (GTC→GTT) | yedJ | putative HD superfamily phosphohydrolase YedJ |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (4/9); total (4/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTG‑ATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATG > NC_000913/2032188‑2032599 | gATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGTATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATTGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTG‑ATCAACACCTTCATTCTcc < 1:256408/251‑1 (MQ=255) gcCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTG‑ATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCgn < 1:75309/251‑2 (MQ=255) ccaccaACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTG‑ATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGcc < 1:89658/251‑1 (MQ=255) cagcagTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGACTCGGTTACTGATATATTTTCCTGCATAGCTTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTG‑ATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTtgt > 1:276564/1‑251 (MQ=255) agTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTG‑ATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGtgcng < 1:225790/251‑1 (MQ=255) ggCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCCCCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCCCCTTGTG‑ATCAACCCCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTGGCCTCGTGCgg < 1:224431/251‑1 (MQ=255) cccGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTG‑ATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGc < 1:235481/250‑1 (MQ=255) gAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTG‑ATCAACACCTTCATTCTCCCCCGCCAGTGCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATTCGCGTTGTGCTGCGCCAgc < 1:92649/216‑1 (MQ=255) gATATATTTTCCTGCTAAGATTGCACCGGCGAGCGGAGATGGCAGACAGGTTAACAAAATGACAGCCATTTAGGGCCCCGCGCTCAGCCAGCGGAGGAAAACGCATCTGTCACCTTGTA‑ATCAACTCCTTCATTCCCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACt < 1:6796/250‑1 (MQ=255) gaTGGCCGACAGGGTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCT‑GTGGATCGCCACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAAGGCGACTAAAAAATGCTCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCAGTAGCTTCAg < 1:111199/208‑1 (MQ=255) gACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTG‑ATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGc > 1:149099/1‑251 (MQ=255) ggTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATAAAATCGCATCTATCACCTTGTG‑ATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATg < 1:208786/251‑1 (MQ=255) nccATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTG‑ATCAACACCTTc > 1:56114/2‑67 (MQ=255) cTCAGNCANCNGANNANAACGNATCTATCACCTTGTG‑TTCAACACCTTCATTCTCCCCCGnc > 2:149254/1‑62 (MQ=255) | GATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTG‑ATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATG > NC_000913/2032188‑2032599 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |