Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	581,440	C→G	intergenic (+61/‑94)	nohD → / → tfaD	putative DNA‑packaging protein NohD/putative tail fiber assembly protein TfaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	581,440	0	C	G	91.7%	62.4 / ‑0.6	24	intergenic (+61/‑94)	nohD/tfaD	putative DNA‑packaging protein NohD/putative tail fiber assembly protein TfaD
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base G (9/13);  total (9/15)
Fisher's exact test for biased strand distribution p-value = 5.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

CGTTTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTC‑CGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGCCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTGGGTGTTTATGC  >  NC_000913/581248‑581625                                                                                                                                                                                                  |                                                                                                                                                                                          cgcttcccGGAGCTGGAAAACCGACATATTGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCG‑CGCGCGGGTCTACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAATGGGCAGACGATAATTACTATCTCCCCAAAGAGTCGGCCTACCAGGa                                                                                                                                   >  1:138083/7‑249 (MQ=255)        cGGAGCTGGAAAACCGACATATTGATTTCCTCAAGAAGGACAGCATGAAAGCCATGAACAAAGCAGCTGCGCGGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCG‑CGCGCGGGTCTACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAATGGTCAGACGATAATTACTATCTCCCCAAAGAGTCGGCCTACCAGGAAGGGCGCTg                                                                                                                          <  1:88546/251‑1 (MQ=255)             ntGGAAAACCGACATATTGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCCGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCG‑CGCGCGGGTCTACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAATGGGCAGACGATAATTACTATCTCCCCAAAGagtcg                                                                                                                                              >  1:37707/2‑222 (MQ=9)               ggAAAACCGACATATTGATTTCCTCAAGAAGGACATCATTAAAGCCATGCCCAAAGCAGCTGAGCTGGATGAAATCATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCG‑CGCGCGGGTATACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAATGGGCCGACGATAATTACTATTTACCCAAAGAGTCGGCCTACCAGGACGGGCGCTGGGAAacg                                                                                                                   >  1:128682/1‑250 (MQ=255)                     ccGACATATTGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCG‑CGCGCGGGTCTACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAATGGGCAGACGATAATTACTATCTCCCCAAAGAGTCGGCCTACCAGGAAGGGCGCTGGGAAacgttgccg                                                                                                             >  1:5438/1‑244 (MQ=255)                               gatttcctcaagaaggacatcattAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCG‑CGCGCGGGTCTACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAATGGGCAGACGATAATTACTATCTCCCCAAAGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCaacgcg                                                                                                   >  1:56285/19‑246 (MQ=255)                               gatttcctcaagaaggacatcattAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCT‑TCGCGCGCGGGTCTACTTGCGCTGTACCGACCTGGGTCTCAAACGGCGTTTGAATGGGCAGACGATAATTACTATCTCCCCAAAGAGTCGGCGTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCaacgc                                                                                                    <  1:172111/232‑5 (MQ=255)                                        aagaaggtcatcattAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTAAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCG‑CGCGCGGGTCTACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAATGGGCAGACGATAATTACTATCTCCCCAAAGAGTCGGCCTACCAGGAAGGGCGCTGGTAAACGTTGCCGTTTCAACGCGCGATCATgn                                                                                          <  1:105192/242‑2 (MQ=255)                                           aaggacatcattAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCG‑CGCGCGGGTCTACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAATGGGCAGACGATAATTACTATCTCCCCAAAGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATg                                                                                       <  1:70962/245‑1 (MQ=255)                                             ggacatcattAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCG‑CGCGCGGGTCTACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAATGGGCAGACGATAATTACTATCTCCCCAAAGAGTCGGCCTACCAGGAAGGGCGCCGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCg                                                                                     >  1:12102/5‑251 (MQ=255)                                                       aaaGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCG‑CGCGCGGGTCTACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAATGGGCAGACGATAATGACTATCTCCCCAAAGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATgggtaac                                                                            <  1:162386/250‑5 (MQ=255)                                                          nccATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCG‑CGCGCGGGTCTACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAATGGGCAGACGATAATTACTATCTCCCCAAAGAGTCAGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCCACGCGCGATCATGAATGCGATgggtaacgatt                                                                        >  1:140965/2‑243 (MQ=255)                                                            cATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCG‑CGCGCGGGTCTACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAATGGGCAGACGATAATTACTATCTCCCCAAAGagtcggcctacc                                                                                                                                       <  1:134201/186‑12 (MQ=255)                                                                               cTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCG‑CGCGCGGGTCTACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAAGGGGCAGACGAGAATTACTATCGCCCCAAAGAGTCGTCCTACCAGGGAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATg                                                                                  <  1:115158/220‑1 (MQ=255)                                                                                    tgaaataaTACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCG‑CGCGCGGGTCTACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAATGGGCAGACGATAATTACTATCTCCCCAAAGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCaacn                                                                                                     <  1:35877/189‑4 (MQ=255)                                                                                          aaTACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCG‑CGCGCGGGTCTACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAATGGGCAGACGATAATTACTATCTCCCCAAAGAGTCGGCCTACCAGGAATGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGc                                         <  1:167903/249‑1 (MQ=255)                                                                                              ncGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCG‑CGCGCGGGTCTACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAATGGGCAGACGATAATTACTATCTCCCCAAAGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGGTGCCGTTTCAACGCGCGATCATGAATGCGATggggaaa                                                                            >  1:34213/2‑207 (MQ=255)                                                                                              ccGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCG‑CGCGCGGGTCTACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAATGGGCAGACGATAATTACTATCTCCCCAAAGAGTCGGCCTACCAGGAAGGGCGCTggn                                                                                                                        <  1:104537/167‑2 (MQ=255)                                                                                               cGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCGGCGGCATTTTGGC‑CGCGCCGGGCTTCGCTCACTGGTCAGGCCGGAGCCACAGACCGCCGTTGAATGGGCAGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCGGGGAAACACTGCCCTGTCAGCGGGGCATCAGGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCgtgt                                   <  1:94806/251‑1 (MQ=255)                                                                                                 gggTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCG‑CGCGCGGGTCTACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAATGGGCAGACGATAATTACTATCTCCCCAAAGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCgtgttn                                 <  1:103057/251‑3 (MQ=255)                                                                                                   gTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCG‑CGCGCGGGTCTACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAATGGGCAGACGATAATTACTATCTCCCCAAAGagtcggc                                                                                                                                            >  1:61100/1‑136 (MQ=255)                                                                                                         gAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCG‑CGCGCGGGTCTACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAATGGGCAGACGATAATTACTATCTCCCCAAAGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGTTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCgtgttgtctattc                          <  1:120634/250‑10 (MQ=255)                                                                                                         gAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCG‑CGCGCGGGTCTACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAAGGGGCAGACGATAATTACTATCTCCCCAAAGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATTCGTTAGGTCAACGTTTTTAAGTCTGCCCGTGTTGGCTAtt                           <  1:158885/249‑1 (MQ=255)                                                                                                                                                                                  ggCCGGAGCCACAGACCGCCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAAGCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGGCATCATGAATGCGATGTGCTGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTTCACGTGTCGTTTATTCCAAAAGGCTGCTGGGTGTTTATGc  <  1:145066/202‑1 (MQ=255)                                                                                                                                                                                                  |                                                                                                                                                                                          CGTTTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTC‑CGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGCCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTGGGTGTTTATGC  >  NC_000913/581248‑581625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑