Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 581,275 G→A V148I (GTT→ATT)  nohD → putative DNA‑packaging protein NohD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913581,2750GA80.0% 29.4 / 6.9 25V148I (GTT→ATT) nohDputative DNA‑packaging protein NohD
Reads supporting (aligned to +/- strand):  ref base G (3/2);  new base A (11/9);  total (14/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.35e-01

GCTGCAGGCCAGCGAGACAGATCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGA‑TCCCCCTGTCGGTGCAGCGGCGTTTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAA‑ACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGCCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCC  >  NC_000913/581040‑581518
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                    
gctgcAGGCCAGCGAGACAGATCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGA‑TCCCCCTGTCGGTGCAGCGGCGTTTTCCGGAACTGGAAAACCGACATGTTGATTTCATGAA‑Ac                                                                                                                                                                                                                                      >  1:122829/1‑251 (MQ=17)
                   gATCTCCAGCCAGGGATTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGAGAAACTGCCAGTATTCTCGACGGGA‑TCCCCCTGTCGGTGCAGCGGCGTTTTCCGGAACTGGAAACCCGACATGTTGATTTCCTGAA‑ACGGGATATCATCAAAGCCAt                                                                                                                                                                                                                   >  1:175336/1‑251 (MQ=9)
                                            aCGAACGCCATTGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGA‑TCCCCCTGTCGGTGCAGCGGCGTTTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAA‑ACGGGATATCATCAAAg                                                                                                                                                                                                                       <  1:3542/222‑1 (MQ=14)
                                                                                   aggaactaaaaaaTGCAAAAGATTCCGCTGAGGTGGTGGAAACCGCATTCTGCACGTTCGTGCTGTCGAGGATGGCCGGAGACGTAGCCAGCATTCTTGATGGAG‑TTCCTCTGTCGGTTCAGCGGCGCTTCCCGGAGCTGGAAAACCGACATATTGATTTCCTCAA‑GAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTc                                                                                                                                                    >  1:77666/9‑250 (MQ=255)
                                                                                              aatgcaaaagattCCGCTGAGGTGGTGGAAACCGCATTCTGCACGTTCGTGCTGTCGCGGATGGCCGGAGAAGTAGCCAGCATTCTTTATGGAG‑TTCCTCTGTCGGTTCAGCGGCGCTTCCCGGAGCTGGAAAACCGACATATTGATTTCCTCAA‑GAAGGACAGCATTAAAGCCATGAACAAATCAGCTGCGCTGGATGAAATAATACCGTGGTTGCTGAGTGAATATATCGAACAGTCAGGTTaaggggc                                                                                                                                        <  1:120193/239‑6 (MQ=255)
                                                                                                    aaagattCCGCTGAGGTGGTGGAAACCGCATTCTGCACGTTCGTGCTGTCGCGGATGGCCGGAGAAGTAGCCAGCATTCTTGATGGAG‑TTCCTCTGTCGGTTCAGCGGCGCTTCCCGGAGCTGGAAAACCGACATATTGATTTCCTCAA‑GAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGcagc                                                                                                                                  <  1:41001/245‑4 (MQ=2)
                                                                                                        attCCGCTGAGGTGGTGGAAACCGCATTCTGCACGTTCGTGCTGTCGCGGATGGCCGGAGAAGTAGCCAGCATTCTTGATGGAG‑TTCCTCTGTCGGTTCAGCGGCGCTTCCCGGAGCTGGAAAACCGACATATTGATTTCCTCAA‑GAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTaaggggcttcagcacnc                                                                                                                              <  1:136550/249‑16 (MQ=2)
                                                                                                            cGCTGAGGTGGTGGAAACCGCATTCTGCACGTTCGTGCTGTCGCGGATGGCCGGAGAAGTAGCCAGCATTCTTGATGGAG‑TTCCTCTGTCGGTTCAGCGGCGCTTCCCGGAGCTGGAAAACCGACATATTGATTTCCTCAA‑GAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTgcagcactctgcg                                                                                                                          <  1:72937/251‑8 (MQ=2)
                                                                                                                naaGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGA‑TCCCCCTGTCGGTGCAGCGGCGTTTTCCGGAACTGGACACCCGACATGTTGATTTCCTGAA‑ACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGaaa                                                                                                                                                        >  1:22387/2‑216 (MQ=17)
                                                                                                                   ggggtggAAACCGCATTCTGCACGTTCGTGCTGTCGCGGATGGCCGGAGAAGTAGCCAGCATTCTTGATGGAG‑TTCCTCTGTCGGTTCAGCGGCGCTTCCCGGAGCTGGAAAACCGACATATTGATTTCCTCAA‑GAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTgcagcactctgcgcgcgcgg                                                                                                                   >  1:159252/3‑237 (MQ=255)
                                                                                                                    tggtggAAACCGCATTCTGCACGTTCGTGCTGTCGCGGATGGCCGGAGAAGTAGCCAGCATTCTTGATGGAG‑TTCCTCTGTCGGTTCAGCGGCGCTTCCCGGAGCTGGAAAACCGACATATTGATTTCCTCAA‑GAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTgcagcactctgcgcgcgcggg                                                                                                                  >  1:13835/1‑236 (MQ=255)
                                                                                                                      gtggAAACCGCATTCTGCACGTTCGTGCTGTCGCTGATGGCCGGAGAAGAAGCCAGCATTCTTGATGGAG‑TTCCTCTGTCGGTTCAGCGGCGCTTCCCGGAGCTGGAAAACCGACATATTGATTTCCTCAA‑GAAGGCCATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTgcagcactctgcgcgcgcgggtc                                                                                                                >  1:39174/1‑234 (MQ=2)
                                                                                                                        ggAAACCGCATTCTGCACGTTCGTGCTGTCGCGGATGGCCAGAGAAGTAGCCAACATTCTTGATGGAG‑TTCCTCTGTCGGTTCAGCGGAGCTTCCCGGAGCTGGAAAACCGACATATTGATTTCCTCAA‑GAAGGACATCATTAAAGCCATGAACAAAGCCGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTgcagcactctgcgcgcgcgggtct                                                                                                               >  1:98379/1‑232 (MQ=255)
                                                                                                                             ccGCATTCTGCACGTTCGTGCTGTCGCGGATGGCCGGAGAAGTAGCCAGCATTCTTGATGGAG‑TTCCTCTGTCGGTTCAGCGGCGCTTCCCGGAGCTGGAAAACCGACATATTGATTTCCTCAA‑GAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTgcagcactctgcgcgcgcgggtctactttc                                                                                                         <  1:50260/251‑25 (MQ=255)
                                                                                                                             ccGCAGTCTGCACGTTCGTGCTGTCGCGGATGGCCGGAGAAGTAGCCAGCATTCTTGATGGAG‑TTCCTCTGTCGGTTCAGCGGCGCTTCCCGGAGCTGGAAAACCGACATAGTGATTTCCTCAA‑GAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTGGCGGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCGGCactctgcgctctcgggtcttctttc                                                                                                         <  1:135619/251‑25 (MQ=255)
                                                                                                                              cGCATTCTGCACGTTCGTGCTGTCGCGGATGGCCGGAGAAGTAGCCAGCATTCTTG‑CTGGAGTTCCTCTGTCGGTTCAGCGGCGCTTCCCGGAGCTGGAACACCGACATATTGATTTCCTCAA‑GAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAACTAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTgcagcac                                                                                                                                >  1:94972/1‑226 (MQ=2)
                                                                                                                                 aTTCTGCACGTTCGTGCTGTCGCGGATGGCCGGAGAAGTAGCCAGCATTCTTGATGGAG‑TTCCTCTGTCGGTTCAGCGGCGCTTCCCGGAGCTGGAAAACCGACATATTGATCTCCTCAA‑GAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCGGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTgcagcactctgcgcgcgcggg                                                                                                                  <  1:121768/238‑16 (MQ=255)
                                                                                                                                                                             gTATTCTCGACGGGA‑TCCCCCTGTCGGTGCAGCGGCGTTTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAA‑ACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAgg                                                                                               <  1:50281/213‑1 (MQ=255)
                                                                                                                                                                                                                 cgcttcccGGAGCTGGAAAACCGACATATTGATTTCCTCAA‑GAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCGCGCGCGGGTCTACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAATGGGCAGACGATAATTACTATCTCCCCAAAGAGTCGGCCTACCAGGa                        >  1:138083/7‑249 (MQ=255)
                                                                                                                                                                                                                   nttcccGGAGCTGGAAAACCGACATATTGATTTCCTCAAGACGG‑ACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGGTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGcag                                                                                                                                   >  1:103802/5‑138 (MQ=255)
                                                                                                                                                                                                                   nnncccNGAGCTGGAAAACCGACATATTGATTTCCTCAA‑GAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGcac                                                                                                                                   <  2:103802/136‑3 (MQ=9)
                                                                                                                                                                                                                        cGGAGCTGGAAAACCGACATATTGATTTCCTCAA‑GAAGGACAGCATGAAAGCCATGAACAAAGCAGCTGCGCGGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCGCGCGCGGGTCTACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAATGGTCAGACGATAATTACTATCTCCCCAAAGAGTCGGCCTACCAGGAAGGGCGCTg               <  1:88546/251‑1 (MQ=255)
                                                                                                                                                                                                                             ntGGAAAACCGACATATTGATTTCCTCAA‑GAAGGACATCATTAAAGCCATGAACAAAGCAGCCGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCGCGCGCGGGTCTACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAATGGGCAGACGATAATTACTATCTCCCCAAAGagtcg                                   >  1:37707/2‑222 (MQ=9)
                                                                                                                                                                                                                               ggAAAACCGACATATTGATTTCCTCAA‑GAAGGACATCATTAAAGCCATGCCCAAAGCAGCTGAGCTGGATGAAATCATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCGCGCGCGGGTATACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAATGGGCCGACGATAATTACTATTTACCCAAAGAGTCGGCCTACCAGGACGGGCGCTGGGAAacg        >  1:128682/1‑250 (MQ=255)
                                                                                                                                                                                                                                     ccGACATATTGATTTCCTCAA‑GAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCGCGCGCGGGTCTACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAATGGGCAGACGATAATTACTATCTCCCCAAAGAGTCGGCCTACCAGGAAGGGCGCTGGGAAacgttgccg  >  1:5438/1‑244 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                    
GCTGCAGGCCAGCGAGACAGATCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGA‑TCCCCCTGTCGGTGCAGCGGCGTTTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAA‑ACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGCCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCC  >  NC_000913/581040‑581518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑