Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 581,202 T→A G123G (GGT→GGA)  nohD → putative DNA‑packaging protein NohD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913581,2020TA82.3% 18.7 / 0.9 17G123G (GGT→GGA) nohDputative DNA‑packaging protein NohD
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base A (7/7);  total (9/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.13e-01

GCTGCAGGCCAGCGAGACAGATCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGA‑TCCCCCTGTCGGTGCAGCGGCGTTTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTC  >  NC_000913/581040‑581415
                                                                                                                                                                  |                                                                                                                                                                                                                      
gctgcAGGCCAGCGAGACAGATCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGA‑TCCCCCTGTCGGTGCAGCGGCGTTTTCCGGAACTGGAAAACCGACATGTTGATTTCATGAAAc                                                                                                                               >  1:122829/1‑251 (MQ=17)
                   gATCTCCAGCCAGGGATTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGAGAAACTGCCAGTATTCTCGACGGGA‑TCCCCCTGTCGGTGCAGCGGCGTTTTCCGGAACTGGAAACCCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCAt                                                                                                            >  1:175336/1‑251 (MQ=9)
                                            aCGAACGCCATTGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGA‑TCCCCCTGTCGGTGCAGCGGCGTTTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAg                                                                                                                <  1:3542/222‑1 (MQ=14)
                                                                                   aggaactaaaaaaTGCAAAAGATTCCGCTGAGGTGGTGGAAACCGCATTCTGCACGTTCGTGCTGTCGAGGATGGCCGGAGACGTAGCCAGCATTCTTGATGGAG‑TTCCTCTGTCGGTTCAGCGGCGCTTCCCGGAGCTGGAAAACCGACATATTGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTc                                             >  1:77666/9‑250 (MQ=255)
                                                                                              aatgcaaaagattCCGCTGAGGTGGTGGAAACCGCATTCTGCACGTTCGTGCTGTCGCGGATGGCCGGAGAAGTAGCCAGCATTCTTTATGGAG‑TTCCTCTGTCGGTTCAGCGGCGCTTCCCGGAGCTGGAAAACCGACATATTGATTTCCTCAAGAAGGACAGCATTAAAGCCATGAACAAATCAGCTGCGCTGGATGAAATAATACCGTGGTTGCTGAGTGAATATATCGAACAGTCAGGTTaaggggc                                 <  1:120193/239‑6 (MQ=255)
                                                                                                    aaagattCCGCTGAGGTGGTGGAAACCGCATTCTGCACGTTCGTGCTGTCGCGGATGGCCGGAGAAGTAGCCAGCATTCTTGATGGAG‑TTCCTCTGTCGGTTCAGCGGCGCTTCCCGGAGCTGGAAAACCGACATATTGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGcagc                           <  1:41001/245‑4 (MQ=2)
                                                                                                        attCCGCTGAGGTGGTGGAAACCGCATTCTGCACGTTCGTGCTGTCGCGGATGGCCGGAGAAGTAGCCAGCATTCTTGATGGAG‑TTCCTCTGTCGGTTCAGCGGCGCTTCCCGGAGCTGGAAAACCGACATATTGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTaaggggcttcagcacnc                       <  1:136550/249‑16 (MQ=2)
                                                                                                            cGCTGAGGTGGTGGAAACCGCATTCTGCACGTTCGTGCTGTCGCGGATGGCCGGAGAAGTAGCCAGCATTCTTGATGGAG‑TTCCTCTGTCGGTTCAGCGGCGCTTCCCGGAGCTGGAAAACCGACATATTGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTgcagcactctgcg                   <  1:72937/251‑8 (MQ=2)
                                                                                                                naaGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGA‑TCCCCCTGTCGGTGCAGCGGCGTTTTCCGGAACTGGACACCCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGaaa                                                 >  1:22387/2‑216 (MQ=17)
                                                                                                                   ggggtggAAACCGCATTCTGCACGTTCGTGCTGTCGCGGATGGCCGGAGAAGTAGCCAGCATTCTTGATGGAG‑TTCCTCTGTCGGTTCAGCGGCGCTTCCCGGAGCTGGAAAACCGACATATTGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTgcagcactctgcgcgcgcgg            >  1:159252/3‑237 (MQ=255)
                                                                                                                    tggtggAAACCGCATTCTGCACGTTCGTGCTGTCGCGGATGGCCGGAGAAGTAGCCAGCATTCTTGATGGAG‑TTCCTCTGTCGGTTCAGCGGCGCTTCCCGGAGCTGGAAAACCGACATATTGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTgcagcactctgcgcgcgcggg           >  1:13835/1‑236 (MQ=255)
                                                                                                                      gtggAAACCGCATTCTGCACGTTCGTGCTGTCGCTGATGGCCGGAGAAGAAGCCAGCATTCTTGATGGAG‑TTCCTCTGTCGGTTCAGCGGCGCTTCCCGGAGCTGGAAAACCGACATATTGATTTCCTCAAGAAGGCCATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTgcagcactctgcgcgcgcgggtc         >  1:39174/1‑234 (MQ=2)
                                                                                                                        ggAAACCGCATTCTGCACGTTCGTGCTGTCGCGGATGGCCAGAGAAGTAGCCAACATTCTTGATGGAG‑TTCCTCTGTCGGTTCAGCGGAGCTTCCCGGAGCTGGAAAACCGACATATTGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCCGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTgcagcactctgcgcgcgcgggtct        >  1:98379/1‑232 (MQ=255)
                                                                                                                             ccGCATTCTGCACGTTCGTGCTGTCGCGGATGGCCGGAGAAGTAGCCAGCATTCTTGATGGAG‑TTCCTCTGTCGGTTCAGCGGCGCTTCCCGGAGCTGGAAAACCGACATATTGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTgcagcactctgcgcgcgcgggtctactttc  <  1:50260/251‑25 (MQ=255)
                                                                                                                             ccGCAGTCTGCACGTTCGTGCTGTCGCGGATGGCCGGAGAAGTAGCCAGCATTCTTGATGGAG‑TTCCTCTGTCGGTTCAGCGGCGCTTCCCGGAGCTGGAAAACCGACATAGTGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTGGCGGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCGGCactctgcgctctcgggtcttctttc  <  1:135619/251‑25 (MQ=255)
                                                                                                                              cGCATTCTGCACGTTCGTGCTGTCGCGGATGGCCGGAGAAGTAGCCAGCATTCTTG‑CTGGAGTTCCTCTGTCGGTTCAGCGGCGCTTCCCGGAGCTGGAACACCGACATATTGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAACTAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTgcagcac                         >  1:94972/1‑226 (MQ=2)
                                                                                                                                 aTTCTGCACGTTCGTGCTGTCGCGGATGGCCGGAGAAGTAGCCAGCATTCTTGATGGAG‑TTCCTCTGTCGGTTCAGCGGCGCTTCCCGGAGCTGGAAAACCGACATATTGATCTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCGGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTgcagcactctgcgcgcgcggg           <  1:121768/238‑16 (MQ=255)
                                                                                                                                                                  |                                                                                                                                                                                                                      
GCTGCAGGCCAGCGAGACAGATCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGA‑TCCCCCTGTCGGTGCAGCGGCGTTTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTC  >  NC_000913/581040‑581415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑