Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	581,548	0	C	T	46.7%	‑2.3 / 11.8	15	G5G (GGC→GGT)	tfaD	putative tail fiber assembly protein TfaD
Reads supporting (aligned to +/- strand):  ref base C (4/4);  new base T (2/5);  total (6/9)
Fisher's exact test for biased strand distribution p-value = 6.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.69e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGCCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTGGGTGTTTATGCCTACTTTATAGAGCATAAGCAGCGCAACACCCTTATTCCAGCTGGCTTCGTGGCTGTTTTCAACAGTG  >  NC_000913/581337‑581693                                                                                                                                                                                                                    |                                                                                                                                                  aaTACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCGCGCGCGGGTCTACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAATGGGCAGACGATAATTACTATCTCCCCAAAGAGTCGGCCTACCAGGAATGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGc                                                                                                             <  1:167903/249‑1 (MQ=255)      cGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCGGCGGCATTTTGGCCGCGCCGGGCTTCGCTCACTGGTCAGGCCGGAGCCACAGACCGCCGTTGAATGGGCAGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCGGGGAAACACTGCCCTGTCAGCGGGGCATCAGGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCgtgt                                                                                                       <  1:94806/251‑1 (MQ=255)        gggTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCGCGCGCGGGTCTACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAATGGGCAGACGATAATTACTATCTCCCCAAAGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCgtgttn                                                                                                     <  1:103057/251‑3 (MQ=255)                gAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCGCGCGCGGGTCTACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAATGGGCAGACGATAATTACTATCTCCCCAAAGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGTTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCgtgttgtctattc                                                                                              <  1:120634/250‑10 (MQ=255)                gAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCGCGCGCGGGTCTACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAAGGGGCAGACGATAATTACTATCTCCCCAAAGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATTCGTTAGGTCAACGTTTTTAAGTCTGCCCGTGTTGGCTAtt                                                                                               <  1:158885/249‑1 (MQ=255)                                                                                        ggCCGGAGCCACAGACCGCCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAAGCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGGCATCATGAATGCGATGTGCTGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTTCACGTGTCGTTTATTCCAAAAGGCTGCTGGGTGTTTATGc                                                                      <  1:145066/202‑1 (MQ=255)                                                                                                        ggcggTTGAATGGGCAGACGATAATTNCTATCTNNCCNAAGAGTCGGCCTACCAGGANGGNNNCNNNGNCACGTNGCNNTTTCAACGCNCGATCATGAATGCGATGGGTAACGATTACCTACGTGAGGTCAACGTTGTTAAGNCTGCCCGTGNNGGCTATTCAAAAATGTTGCTGNGTGNNTNNgngtangtn                                                               >  2:42699/5‑185 (MQ=255)                                                                                                            nttGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGGGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTGGGTGTTTATGCCTACTTTATAGAGCATAAGCAGCGCAACACCCTTAGTCCAGCTGGCTTCGTGGCTGTTTTCAACAGtg  >  1:108569/2‑250 (MQ=255)                                                                                                                           gatAATTACTATCTCCCCAAAGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTTAGGTCAACGTTGTTAAGTCTGCCCGTGNTGGNNNNNc                                                                                              >  2:110848/3‑143 (MQ=255)                                                                                                                                                      nnnnaCCNGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTGGGTGTTTATGcc                                                                     <  2:104174/137‑1 (MQ=255)                                                                                                                                                      ncATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTGGGTGTTTATGcc                                                                     >  1:104174/2‑141 (MQ=255)                                                                                                                                                                   ggCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCg                                                                                                     >  1:48689/1‑96 (MQ=255)                                                                                                                                                                                                  gatcatGAATGCGATGGGTANCGANTACANACNTGAGGTCAACGTTGTNAAGTCTGCCCGTGTTGGCTATTCAAAAATGTTGCTGGGTGTTTANNCNNNTNTtattcag                                                         <  2:120599/108‑5 (MQ=255)                                                                                                                                                                                                              gATGGGCANCGAANANNNCCGTGNGGTGAATGTNGTGAAGTCTGCCCGTGTCGNt                                                                                                   >  2:19057/1‑55 (MQ=255)                                                                                                                                                                                                              gATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCggn                                                                                                   <  1:19057/55‑2 (MQ=255)                                                                                                                                                                                                                    |                                                                                                                                                  GATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGCCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTGGGTGTTTATGCCTACTTTATAGAGCATAAGCAGCGCAACACCCTTATTCCAGCTGGCTTCGTGGCTGTTTTCAACAGTG  >  NC_000913/581337‑581693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑