Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	581,530	0	C	G	70.0%	17.6 / 12.8	20	intergenic (+151/‑4)	nohD/tfaD	putative DNA‑packaging protein NohD/putative tail fiber assembly protein TfaD
Reads supporting (aligned to +/- strand):  ref base C (3/3);  new base G (5/9);  total (8/12)
Fisher's exact test for biased strand distribution p-value = 6.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.43e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGCCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTGGGTGTTTATGCCTACTTTATAGAGCATAAGCAGCGCAACACCCTTATTCCAGCTGGCTTCGTGGCTGTTTTCAACAGTG  >  NC_000913/581287‑581693                                                                                                                                                                                                                                                    |                                                                                                                                                                    aagaaggtcatcattAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTAAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCGCGCGCGGGTCTACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAATGGGCAGACGATAATTACTATCTCCCCAAAGAGTCGGCCTACCAGGAAGGGCGCTGGTAAACGTTGCCGTTTCAACGCGCGATCATgn                                                                                                                                                              <  1:105192/242‑2 (MQ=255)    aaggacatcattAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCGCGCGCGGGTCTACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAATGGGCAGACGATAATTACTATCTCCCCAAAGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATg                                                                                                                                                           <  1:70962/245‑1 (MQ=255)      ggacatcattAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCGCGCGCGGGTCTACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAATGGGCAGACGATAATTACTATCTCCCCAAAGAGTCGGCCTACCAGGAAGGGCGCCGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCg                                                                                                                                                         >  1:12102/5‑251 (MQ=255)                aaaGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCGCGCGCGGGTCTACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAATGGGCAGACGATAATGACTATCTCCCCAAAGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATgggtaac                                                                                                                                                <  1:162386/250‑5 (MQ=255)                   nccATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCGCGCGCGGGTCTACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAATGGGCAGACGATAATTACTATCTCCCCAAAGAGTCAGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCCACGCGCGATCATGAATGCGATgggtaacgatt                                                                                                                                            >  1:140965/2‑243 (MQ=255)                                        cTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCGCGCGCGGGTCTACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAAGGGGCAGACGAGAATTACTATCGCCCCAAAGAGTCGTCCTACCAGGGAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATg                                                                                                                                                      <  1:115158/220‑1 (MQ=255)                                                   aaTACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCGCGCGCGGGTCTACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAATGGGCAGACGATAATTACTATCTCCCCAAAGAGTCGGCCTACCAGGAATGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGc                                                                                                             <  1:167903/249‑1 (MQ=255)                                                       ncGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCGCGCGCGGGTCTACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAATGGGCAGACGATAATTACTATCTCCCCAAAGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGGTGCCGTTTCAACGCGCGATCATGAATGCGATggggaaa                                                                                                                                                >  1:34213/2‑207 (MQ=255)                                                        cGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCGGCGGCATTTTGGCCGCGCCGGGCTTCGCTCACTGGTCAGGCCGGAGCCACAGACCGCCGTTGAATGGGCAGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCGGGGAAACACTGCCCTGTCAGCGGGGCATCAGGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCgtgt                                                                                                       <  1:94806/251‑1 (MQ=255)                                                          gggTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCGCGCGCGGGTCTACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAATGGGCAGACGATAATTACTATCTCCCCAAAGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCgtgttn                                                                                                     <  1:103057/251‑3 (MQ=255)                                                                  gAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCGCGCGCGGGTCTACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAATGGGCAGACGATAATTACTATCTCCCCAAAGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGTTAACGATTACATACGTGAGGTCAACGTTGTTAAGTCTGCCCgtgttgtctattc                                                                                              <  1:120634/250‑10 (MQ=255)                                                                  gAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGCACTCTGCGCGCGCGGGTCTACTTTCGCTGTACCGACCTGAGCCGCAAACGGCGGTTGAAGGGGCAGACGATAATTACTATCTCCCCAAAGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATTCGTTAGGTCAACGTTTTTAAGTCTGCCCGTGTTGGCTAtt                                                                                               <  1:158885/249‑1 (MQ=255)                                                                                                                                          ggCCGGAGCCACAGACCGCCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAAGCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGGCATCATGAATGCGATGTGCTGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTTCACGTGTCGTTTATTCCAAAAGGCTGCTGGGTGTTTATGc                                                                      <  1:145066/202‑1 (MQ=255)                                                                                                                                                          ggcggTTGAATGGGCAGACGATAATTNCTATCTNNCCNAAGAGTCGGCCTACCAGGANGGNNNCNNNGNCACGTNGCNNTTTCAACGCNCGATCATGAATGCGATGGGTAACGATTACCTACGTGAGGTCAACGTTGTTAAGNCTGCCCGTGNNGGCTATTCAAAAATGTTGCTGNGTGNNTNNgngtangtn                                                               >  2:42699/5‑185 (MQ=255)                                                                                                                                                             ggTTGAATGGGCAGACNATANTTACNATNTCCCCAAAGAGTCTGNCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGANNANNNANTCGATggg                                                                                                                                                    <  2:125770/104‑1 (MQ=255)                                                                                                                                                              nttGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGGGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTGGGTGTTTATGCCTACTTTATAGAGCATAAGCAGCGCAACACCCTTAGTCCAGCTGGCTTCGTGGCTGTTTTCAACAGtg  >  1:108569/2‑250 (MQ=255)                                                                                                                                                                             gatAATTACTATCTCCCCAAAGAGTCGGCCTACCAGGAAGGGCGCTGGGAAACGTTGCCGTTTCAACGCGCGATCATGAATGCGATGGGTAACGATTACATACGTTAGGTCAACGTTGTTAAGTCTGCCCGTGNTGGNNNNNc                                                                                              >  2:110848/3‑143 (MQ=255)                                                                                                                                                                                                        nnnnaCCNGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTGGGTGTTTATGcc                                                                     <  2:104174/137‑1 (MQ=255)                                                                                                                                                                                                        ncATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTGGGTGTTTATGcc                                                                     >  1:104174/2‑141 (MQ=255)                                                                                                                                                                                                                     ggCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCg                                                                                                     >  1:48689/1‑96 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                    AAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGCCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGATGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCAAAATGCTGCTGGGTGTTTATGCCTACTTTATAGAGCATAAGCAGCGCAACACCCTTATTCCAGCTGGCTTCGTGGCTGTTTTCAACAGTG  >  NC_000913/581287‑581693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑