| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 367,215 | 0 | A | G | 41.2% | ‑2.8 / 15.4 | 18 | V99A (GTA→GCA) | lacI | DNA‑binding transcriptional repressor LacI |
| Reads supporting (aligned to +/- strand): ref base A (6/4); new base G (4/3); total (11/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.77e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGG > NC_000913/366976‑367448 | gACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTGCCATCGAcacc < 1:12506/251‑1 (MQ=255) cncAATGCGACCAGATGCTCCACGCCCAGTCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGAcaccac > 1:136608/1‑251 (MQ=255) ccAATGCGACCAGATGCTCCACGCCCAGTCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGGCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTGGCGCTAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTGCCATCGAcaccacc < 1:91085/251‑1 (MQ=255) aTGCGACCAGATGCTCCACGCCCAGTCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTTGTCAGATACATCAAGAAATACCGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGTTCATCCAGCGGATAGTTAATGATCAGCCCACTGCCGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACTCCACCACg < 1:157932/251‑1 (MQ=255) cTCCACGCCCAGTCGCGTACCGTCTTCATGGGAGAAAATAATACCGTTGGTGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGGGCAGGCAGCTTCCCCAGCAATGGCCTCCTGGTCATCCAGCGGGTAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGTCCCCGCTTCGTTCTCCCATCGACACCAACACGCTGGAACCCAGtt > 1:160570/1‑251 (MQ=255) cgcgTACCGTCTTCATGGGAGACACTAATACTGTTGATGGGTGTCTGGTCAGAGACATCACGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTATGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTGCCATCGACACCACCACGCTGGCACCCAGTTGATCGGcgcg > 1:146734/1‑248 (MQ=255) gaAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTGCCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAAt > 1:153704/1‑237 (MQ=255) gTTGAGGGGTGGCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGTGTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAAGGACCAGCCCACTTACGCGTTGCGCGAGAAGATTGTGCTCCGCCGCTTTACAGGCTTCGACGCCGCTGCGTTCTACCATCGACACCGCCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGcgcg < 1:157933/250‑1 (MQ=255) gTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTgg > 1:74465/1‑251 (MQ=255) naTCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTGCCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGAc > 1:46302/2‑240 (MQ=255) naaCATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGTACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGCCTGGAGGTGGCAACGCCAATCAGCAACg > 1:45415/2‑249 (MQ=255) aCATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGCTCAGCCTACTGACGCGTTGCGCGAGAAGATTGTGCAACGACGCTTTACAGGCTTCGACGACGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATc > 1:113186/1‑167 (MQ=255) aGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACGGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTGCCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGtttn < 1:22789/250‑2 (MQ=255) tggcagCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGc < 1:5165/249‑1 (MQ=255) gCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGGTTCGACGCCGCTTCGTTCTACCATCGACACAACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGAGCCAGAc > 1:123996/1‑196 (MQ=255) cTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGAAGCAGCTTCGTTCTACCATCGACACCACAACGATGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGACAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACttt > 1:97839/1‑251 (MQ=255) cGACGCCGCTTCGTTCTACCATCGACACCCCCACGCTGGCACCCATTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTGTGCCCGCCAGTTGTTGTGCCACGCGGTGGGGAATGTAATTCAGCTCCGCCATCGCCGCGGCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCgg < 1:163198/251‑1 (MQ=255) gccgcTTCGTTCTGCCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTNTGcn > 2:106924/1‑137 (MQ=255) gaCCAGCGACACCACCACGCTGGCACCCAGGGGATCGGCGCGAGATTAAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACt < 1:154392/89‑1 (MQ=255) | GACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGG > NC_000913/366976‑367448 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |