Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,560,455	+G	intergenic (+589/+167)	rtcR → / ← glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,560,455	1	.	G	100.0%	39.7 / NA	14	intergenic (+589/+167)	rtcR/glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (6/8);  total (6/8)

GATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTG  >  NC_000913/3560240‑3560695                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                 gaTTGTGGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGGGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTGGCTATCGGGCCACGCCGTGTGAACCGAGCTGTAAGGTAGCTCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAgn                                                                                                                                                                                                                <  1:59378/251‑2 (MQ=255)                                                             gcttggtgCCGATACCAATAAACATCGTCGAGCCATTGGGTATTTGCTCCGCCACTTTGGGGGCGATGCTCTCTTTTTCTTCGTTCGGGGTGGCCTTGCGATCGTGCCACGGCGTTTTAACCGACCTGGAAGGAACCGCCGCTCCTCCATTATGGCGCAGGATCAGGTGGTGCCCCGCCAGCGCTTTGAGTTGTCGGCAAATAGTCCGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACa                                                                                                                                                    <  1:275039/248‑1 (MQ=255)                                                                                                    ggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTCCGGTCTGGGAGGCCTTGCGATCGTGCCACGGCGTGTTCACCGAACTGGAAGGCAGCGCCGCACAGCCCTGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTAATTGCGGTCGCGGCGAATAGTCTGCGGGCTGACGGCGAAATGCTCTACCAGCTCTTCGGTACTGCCATAACCCTGCTGTTTAACCAGTTCGATAATACCGTtgtg                                                                                                              >  1:163541/1‑250 (MQ=255)                                                                                                                                   ctctTTTTCTTCGGTCTGGGGGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGGGGCGCACGGCCCTGATGGCGCAGGATCAGGGTTTGCTCCGCCAGCTCATTGAGGTCGGGGCGTATAGTCTGCGGGCTGACGGGGAAATGCTCTACCAGCTCTTCGGTCCTGACACAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGCGTTTTTTTCATTTATACATTCCTgg                                                                              >  1:280278/1‑251 (MQ=255)                                                                                                                                                         nccTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCgcgcct                                                        >  1:70820/2‑249 (MQ=255)                                                                                                                                                         gCCTTTCGATCGGGCCACGGCTTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGATCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTcgc                                                            <  1:288792/247‑1 (MQ=255)                                                                                                                                                             tGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGGGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTg                                                      <  1:164411/249‑1 (MQ=255)                                                                                                                                                                                             gAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTTGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTa                                                                                                                                     <  1:100128/138‑1 (MQ=255)                                                                                                                                                                                                 gCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTa                 >  1:137849/1‑250 (MQ=255)                                                                                                                                                                                                        ngcACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGATCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCACTGGACTTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCg         >  1:32765/2‑251 (MQ=255)                                                                                                                                                                                                        cgcACNNCCNNNATNNCGCAGGATCAGGTTTTGCTCCGCNAGCTCATTGAGGTCGCGGCGACTAGTCTGNGNGNTGACNNAGANATGCTCTACCNGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACNGNTTTGAAGTTGTATTTGTTTCATTTATAAATCNCTGGAATTATTTTCGTTTTCGCTCNtttanncgntnnnnnnnnn                                      >  2:225378/1‑205 (MQ=255)                                                                                                                                                                                                        cgcACCGCCATGAGGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTGTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGc                                                   <  1:225378/209‑1 (MQ=255)                                                                                                                                                                                                              gCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTg                                                      <  1:101771/200‑1 (MQ=255)                                                                                                                                                                                                               ccATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGAtgtgtg  <  1:207814/251‑1 (MQ=255)                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                 GATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTG  >  NC_000913/3560240‑3560695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑