Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,175,991	T→A	K111* (AAG→TAG)	gatZ ←	putative tagatose‑1,6‑bisphosphate aldolase 2 chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,175,991	0	T	A	100.0%	202.9 / NA	63	K111* (AAG→TAG)	gatZ	putative tagatose‑1,6‑bisphosphate aldolase 2 chaperone
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (41/22);  total (41/22)

AGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTTTACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGT  >  NC_000913/2175696‑2176286                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                        aGCCCACGGGCAATAAATGCCTTTTGATGCGTACGTAAAGTATTTGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACGGAAGCCGGCACGAACATATTCCTATAc                                                                                                                                                                                                                                                                                                      <  1:577461/299‑1 (MQ=255) aGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACNGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATAcc                                                                                                                                                                                                                                                                                                     <  1:470178/300‑1 (MQ=255)             aTAAAGGCCTTTTGATGCATACGTAAAGTATTAGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTNCGGTGCCAATGACATAGCTCAGTTGCTCACGCNGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGCATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTCCTGAAGCCGGCACGAACATGTTCCTATCCCAGCTCGACGGAt                                                                                                                                                                                                                                                                                         >  2:492994/1‑300 (MQ=255)                     cTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTATGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACTCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCAt                                                                                                                                                                                                                                                                                 >  1:246560/1‑300 (MQ=255)                          gATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATAccctgtctcttatacacatctcgcag                                                                                                                                                                                                                                                                             <  1:366270/299‑25 (MQ=255)                                        gTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCt                                                                                                                                                                                                                                                              >  1:203210/1‑300 (MQ=255)                                                     cTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGt                                                                                                                                                                                                                                                 >  1:243940/1‑300 (MQ=255)                                                            atgtttGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGccc                                                                                                                                                                                                                                          <  1:265613/295‑1 (MQ=255)                                                              gggTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCAAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCACAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATNGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCATATACCAGCTCGAAGGATTTTTCCACCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCa                                                                                                                                                                                                                                        >  2:212253/1‑300 (MQ=255)                                                                  ggtgtgTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTAATGAAGCCGGCACGAACATATTCCTATATCAGCTCGACGGATTTTTCCATCGCCGCATCCACATTTTCTTGCTGCCAGCAGTTTGGCCCCAgat                                                                                                                                                                                                                                     >  2:371761/3‑299 (MQ=255)                                                                  gATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGTAGTTTGGCCCCAgat                                                                                                                                                                                                                                     >  2:416844/1‑299 (MQ=255)                                                                  gATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAgat                                                                                                                                                                                                                                     >  2:371767/1‑299 (MQ=255)                                                                  gATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAgat                                                                                                                                                                                                                                     >  1:349755/1‑299 (MQ=255)                                                                         actgactgAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGcccccgacgattgcc                                                                                                                                                                                                                              >  1:221631/1‑288 (MQ=255)                                                                                       gcTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGc                                                                                                                                                                                                                <  1:494274/299‑1 (MQ=255)                                                                                          ggCCTCACCGCCCGGANCCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTNTGGCCCCAGATGATCGCCNCCGAGAATAATGTGTTc                                                                                                                                                                                                            >  1:542655/1‑300 (MQ=255)                                                                                          ggCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTc                                                                                                                                                                                                            >  1:505392/1‑300 (MQ=255)                                                                                                           ccggaacTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCCCCCTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTATGGTGCTAACGATATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCAGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCAAGGTGATCGCCGCCGAGAATGATGCGTTCGCGTgacaaccaaac                                                                                                                                                                                             >  2:312828/1‑288 (MQ=255)                                                                                                           ccggaacTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACtt                                                                                                                                                                                           >  1:220017/1‑300 (MQ=255)                                                                                                           ccggaacTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACACAACtt                                                                                                                                                                                           >  2:312692/1‑300 (MQ=255)                                                                                                           ccggaacTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGAAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACGTTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGACGCCGAGAATAATGCGTTCGCGTGCAAACCCAACtt                                                                                                                                                                                           >  1:220519/1‑300 (MQ=255)                                                                                                                        gCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCGTCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGt                                                                                                                                                                              >  2:446145/1‑300 (MQ=255)                                                                                                                        gCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGt                                                                                                                                                                              >  1:348152/1‑300 (MQ=255)                                                                                                                             tGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTaaaaa                                                                                                                                                                         >  1:117445/1‑300 (MQ=255)                                                                                                                               aCATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACa                                                                                                                                                                       >  1:219165/1‑300 (MQ=255)                                                                                                                                  tAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAAt                                                                                                                                                                    >  1:47242/1‑300 (MQ=255)                                                                                                                                                         aTCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCAACCTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCAGGTGTCAt                                                                                                                                             >  2:170677/1‑300 (MQ=255)                                                                                                                                                                   acacTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATaa                                                                                                                                   >  1:610619/1‑300 (MQ=255)                                                                                                                                                                     actgtCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAGCGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTANACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAAc                                                                                                                                  <  2:207431/295‑1 (MQ=255)                                                                                                                                                                     acTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATACCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAAcc                                                                                                                                 <  1:211793/300‑1 (MQ=255)                                                                                                                                                                          ccGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACNGACGCCGGCACGAACATATTCCTATACCAGCTNGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATGGGCAATCGTAAAAACAAATTCGCGCCAGTCTGCCGGTGTCATTCCGGTATAACCGCCaa                                                                                                                            >  2:487952/1‑300 (MQ=255)                                                                                                                                                                            gcagcaAAGCAAAGCACAGCAGCTCGTTCCGCAATCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCTACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGTCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAat                                                                                                                          <  2:189310/300‑1 (MQ=255)                                                                                                                                                                                            cagcagCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATNCTCCTATACCAGCTCGACGGATTTTTCCATCGNCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTTGCGAAAGTCTGCCGGTGTCCTTCCGGTATAACCTCCAAACTGATTGACCTGGttt                                                                                                           >  2:492948/1‑299 (MQ=255)                                                                                                                                                                                              gcagcTCGTTCCGCAACCGTTTCTGGTGCTACCGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGCAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCATAAAACCAAATTCGCGCAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAACTGATTGACCCGGTTTGa                                                                                                         >  2:239474/1‑299 (MQ=255)                                                                                                                                                                                              cccgcTCGTTCCGCAACCGCTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGAAGCATCACGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGCTTTTTACATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAAAATAATGCGTTCGCGTGCAAACCCAACTTTCTCGGCAATCGTACAAACAAAtt                                                                                                                                                                   >  2:239743/4‑241 (MQ=255)                                                                                                                                                                                               cagcTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCAGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTTGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGttt                                                                                                           <  1:192302/296‑1 (MQ=255)                                                                                                                                                                                               cagcTCGTTCCGCAACCGTTTCGGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGtt                                                                                                            <  2:532935/295‑1 (MQ=255)                                                                                                                                                                                                agcTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACg                                                                                                       <  2:434497/299‑1 (MQ=255)                                                                                                                                                                                                 gcTCTTTCCGCAACCGTTTCTTGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACg                                                                                                       <  2:434647/298‑1 (MQ=255)                                                                                                                                                                                                          gCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCAGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCaa                                                                                              <  1:557519/298‑1 (MQ=255)                                                                                                                                                                                                                         gCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGt                                                                              >  1:316931/1‑299 (MQ=255)                                                                                                                                                                                                                              cGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGNCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGtt                                                                        >  1:470283/1‑300 (MQ=255)                                                                                                                                                                                                                              cGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGtt                                                                        >  1:488128/1‑300 (MQ=255)                                                                                                                                                                                                                                gTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCNGTGTCATTCCGGTATAACCGCCACATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGc                                                                      >  1:137571/1‑300 (MQ=255)                                                                                                                                                                                                                                gTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGc                                                                      >  1:74993/1‑300 (MQ=255)                                                                                                                                                                                                                                                   ggACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAGCCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGcgccgc                                                   >  1:249981/1‑300 (MQ=255)                                                                                                                                                                                                                                                   ggACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGcgccgc                                                   >  1:586661/1‑300 (MQ=255)                                                                                                                                                                                                                                                     aCATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCtt                                                 >  1:384569/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  acgATGAATTTTCCTGACGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCCTTTTCTTGCTGCCAGTAGTTTGGCCCCAGATGATTGCCGCCGAGAATAATGCGTTCGCGTGCAGACCCAACTTTGTCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAAACGCAAAATTGATTGAACTGGTTTGACGTTGTTTCAATCAGCACTTTGCGCATGCTGTTGTGATCAAATGCCAGCGCCGCTTCGATAACCAAc                                      >  2:313448/3‑298 (MQ=255)                                                                                                                                                                                                                                                                  aaGATGAATTTTACTTAAGCCGGCACGAACATATTCCTATACCAGCTCTACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTTGCCCCAGATGATCGCCGCCGAGAATAATGCNTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCGAATGCCAGCGCCGCTTCGATAAcc                                         <  2:203210/295‑1 (MQ=255)                                                                                                                                                                                                                                                                              aCTGAAGCCTGCAAAAACACACTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAAc                         <  2:8380/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                 gAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTTGCCCCATATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGCCAGAAc                         <  2:257302/296‑1 (MQ=255)                                                                                                                                                                                                                                                                                       ggCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAAc                <  1:629304/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                            gAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATg           <  1:400273/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                             aacctatTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGcc         >  1:331894/5‑300 (MQ=255)                                                                                                                                                                                                                                                                                             aaCATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTGTAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGcc         <  1:492994/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                             aaCATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGcc         >  1:262942/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                             aaCATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGcc         <  2:219165/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                               cATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGACAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGa       >  2:247333/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                 tatTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTTGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGata     <  1:256450/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                 tatTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGata     <  1:257024/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                   ttCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTACTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACGAATTCGCGAAAGTCTGCCGGTGTCGTTACGGTATAACCGACGAATTGATTGACCTGGTTTGACGTTGTTTCAATCAGCACTTTGTGCGTGCTGTTGCGATCCAATACCAGCGCCGCTTCGATAACCAACGGATGGGAAGAACAGACTGAATATATGCCGatat    >  2:247740/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                                    tCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGt  >  1:260576/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                        AGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTTTACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGT  >  NC_000913/2175696‑2176286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑