Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,123,556	C→T	E167K (GAA→AAA)	grxB ←	reduced glutaredoxin 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,123,556	0	C	T	100.0%	194.8 / NA	61	E167K (GAA→AAA)	grxB	reduced glutaredoxin 2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/41);  total (20/41)

AACTTGTTCATGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTCGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAGTAACGGTTTGCCGTCGAGTTTATCGAC  >  NC_000913/1123263‑1123850                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                       aaCTTGTTCATGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCAtt                                                                                                                                                                                                                                                                                                  >  1:352078/1‑300 (MQ=255)    ttGTTCATGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACATAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGNGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCAc                                                                                                                                                                                                                                                                                               <  2:208616/300‑1 (MQ=255)          aTGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCAGCGAATACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTNGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCAATTCACGGCGtt                                                                                                                                                                                                                                                                                         >  2:211978/1‑300 (MQ=255)           tGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTc                                                                                                                                                                                                                                                                                        >  1:614040/1‑300 (MQ=255)                gTCCTCGACATGAAATNTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGGTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATNATCTTCCGAAAGTTTGCCNTTCACGGCGTTCGGttt                                                                                                                                                                                                                                                                                   >  1:552350/1‑300 (MQ=255)                gTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCATCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGttt                                                                                                                                                                                                                                                                                   <  2:22961/300‑1 (MQ=255)                gTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTCATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGttt                                                                                                                                                                                                                                                                                   >  2:306475/1‑300 (MQ=255)                gTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGttt                                                                                                                                                                                                                                                                                   <  2:238853/300‑1 (MQ=255)                           gAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGtt                                                                                                                                                                                                                                                                        <  1:4160/300‑1 (MQ=255)                             aaTCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGttt                                                                                                                                                                                                                                                                       >  1:64206/1‑299 (MQ=255)                                 tGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTcc                                                                                                                                                                                                                                                                   <  2:545978/299‑1 (MQ=255)                                              tGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAatc                                                                                                                                                                                                                                                     <  1:440307/300‑1 (MQ=255)                                                acacAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAACAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTCATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACTGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAat                                                                                                                                                                                                                                                      >  1:56464/1‑297 (MQ=255)                                                acacAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAatcat                                                                                                                                                                                                                                                   <  2:106859/300‑1 (MQ=255)                                                    aaCGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGc                                                                                                                                                                                                                                                <  2:340603/299‑1 (MQ=255)                                                                 tCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTGATTCCGACTACCAGCGTCAGACTACGCAGTATCTGAAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAGATCATCGCTGATATTCTTAATc                                                                                                                                                                                                                                  <  2:450504/300‑1 (MQ=255)                                                                 tCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATc                                                                                                                                                                                                                                  <  2:450447/300‑1 (MQ=255)                                                                 tCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATc                                                                                                                                                                                                                                  <  1:386827/300‑1 (MQ=255)                                                                           tAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCag                                                                                                                                                                                                                        <  2:590462/300‑1 (MQ=255)                                                                             aaCTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCagag                                                                                                                                                                                                                      <  2:182284/300‑1 (MQ=255)                                                                             aaCTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAAGACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCagag                                                                                                                                                                                                                      <  2:182397/300‑1 (MQ=255)                                                                                tCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTgg                                                                                                                                                                                                                   <  1:507006/300‑1 (MQ=255)                                                                                        agAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGTTCAGGATTAAATCGCCATTGATGATAANAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCcagcag                                                                                                                                                                                                           <  1:120462/300‑1 (MQ=255)                                                                                        agAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCcagcag                                                                                                                                                                                                           <  1:328389/300‑1 (MQ=255)                                                                                         gAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATAGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAgg                                                                                                                                                                                                          <  1:328858/300‑1 (MQ=255)                                                                                           aaaaaCTGAAAACGGCGGCAACCCTCGAATATAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAgg                                                                                                                                                                                                          <  2:26874/298‑1 (MQ=255)                                                                                                 tGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCaa                                                                                                                                                                                                   <  2:562586/299‑1 (MQ=255)                                                                                                                  cgcgAATACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCct                                                                                                                                                                                 >  1:595342/1‑300 (MQ=255)                                                                                                                       aTACAGGCTGCCGCGGNGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCNAGCAGGTCGGCAAAATTANCCGCGCTGGCCtctttc                                                                                                                                                                            >  1:548287/1‑300 (MQ=255)                                                                                                                           atgCTGCCGCGGCGGGTCAGGATTAAATCTCCATTGATGATAACAAATTGATTTGTGTCTTTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGt                                                                                                                                                                        <  2:355875/298‑1 (MQ=255)                                                                                                                            ggCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTc                                                                                                                                                                       >  2:408428/1‑300 (MQ=255)                                                                                                                                         ggTCAGGATGAAATCGCCAGTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCAGTAATCAGCAACGCGTCTTGGCCAGTTAATTCCGGCTACCAGCGTCAAATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTGGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTTTTGTCGACGAAatagtgg                                                                                                                                                          <  2:406801/300‑5 (MQ=255)                                                                                                                                              ggATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTgcgcgc                                                                                                                                                     >  1:623347/1‑300 (MQ=255)                                                                                                                                                        gCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAg                                                                                                                                             <  2:128337/298‑1 (MQ=255)                                                                                                                                                                            gATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGa                                                                                                                       >  1:351566/1‑300 (MQ=255)                                                                                                                                                                                                       gcgGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCATCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGttt                                                                                            <  2:432630/300‑1 (MQ=255)                                                                                                                                                                                                       gcgGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGttt                                                                                            <  2:432680/300‑1 (MQ=255)                                                                                                                                                                                                          gTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTNGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTttgtt                                                                                         >  1:119900/1‑300 (MQ=255)                                                                                                                                                                                                          gTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTAACAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTNGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTttgtt                                                                                         >  1:120342/1‑300 (MQ=255)                                                                                                                                                                                                            aaTCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTttgtt                                                                                         >  1:417463/1‑298 (MQ=255)                                                                                                                                                                                                                   aaCGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGcc                                                                                <  1:345324/300‑1 (MQ=255)                                                                                                                                                                                                                        ggCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGNGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGa                                                                           >  1:131543/1‑300 (MQ=255)                                                                                                                                                                                                                         gCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGAc                                                                          <  1:555974/300‑1 (MQ=255)                                                                                                                                                                                                                         gCTTGGCCAGGTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTCATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGAc                                                                          <  2:526161/300‑1 (MQ=255)                                                                                                                                                                                                                                 aGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTgcgc                                                                   <  1:629682/299‑1 (MQ=255)                                                                                                                                                                                                                                 aGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCa                                                                  >  1:363394/1‑300 (MQ=255)                                                                                                                                                                                                                                        tCCGGCTACCGTCTTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACTGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCActc                                                           <  2:322900/300‑1 (MQ=255)                                                                                                                                                                                                                                        tCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCActc                                                           <  2:322953/300‑1 (MQ=255)                                                                                                                                                                                                                                              tACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACTGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAAt                                                     <  1:109931/300‑1 (MQ=255)                                                                                                                                                                                                                                              tACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAAt                                                     <  1:108985/300‑1 (MQ=255)                                                                                                                                                                                                                                                  aGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATgg                                                   <  1:259642/298‑1 (MQ=255)                                                                                                                                                                                                                                                  aGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGGTTGCCATTCACTGCGTTCGGTTTGACAATCAGTTTGTACATCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTTGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAAt                                                     <  2:39647/296‑1 (MQ=255)                                                                                                                                                                                                                                                   gCGTCAGATTACGCGGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCAATTGACCTTGCGCAGCCACTCTTCAATGGCa                                                 >  2:15596/1‑299 (MQ=255)                                                                                                                                                                                                                                                   gCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATCCACTGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCACGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATgg                                                   <  2:293760/297‑1 (MQ=255)                                                                                                                                                                                                                                                        aGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGaa                                           >  1:246396/1‑300 (MQ=255)                                                                                                                                                                                                                                                           ttACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGt                                        <  1:299422/300‑1 (MQ=255)                                                                                                                                                                                                                                                               gCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGc                                    <  1:211978/300‑1 (MQ=255)                                                                                                                                                                                                                                                                  gTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACTGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCgg                                 <  2:614040/300‑1 (MQ=255)                                                                                                                                                                                                                                                                            tAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGAAGGGGAACGTTTGCCGGTCAGTAACgg                       <  1:562481/300‑1 (MQ=255)                                                                                                                                                                                                                                                                              gCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTCGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAGTAACGGtt                     >  2:55456/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                 aaGTTTGCCATTCACGGCGTTCGTTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGNAAACTCATCAAATGCCGATTTGGCAAAACGCGNCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAGTAACGGTTTGCCGTCGAGTTTATCGAc  <  2:484648/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                       AACTTGTTCATGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTCGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAGTAACGGTTTGCCGTCGAGTTTATCGAC  >  NC_000913/1123263‑1123850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑