Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	553,161	G→A	intergenic (‑61/+57)	purE ← / ← lpxH	N(5)‑carboxyaminoimidazole ribonucleotide mutase/UDP‑2,3‑diacylglucosamine diphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	553,161	0	G	A	100.0%	193.3 / NA	54	intergenic (‑61/+57)	purE/lpxH	N(5)‑carboxyaminoimidazole ribonucleotide mutase/UDP‑2,3‑diacylglucosamine diphosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/29);  total (25/29)

TGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGCTGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCT  >  NC_000913/552866‑553448                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                tGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGTCGAAGCTGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAag                                                                                                                                                                                                                                                                                             <  2:373152/300‑1 (MQ=255)             gcgctgcgcCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGCTGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGaaa                                                                                                                                                                                                                                                                                 >  1:529239/1‑300 (MQ=255)                                           ccTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGCTGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGt                                                                                                                                                                                                                                                   >  1:273713/1‑300 (MQ=255)                                              gATAACCGTTCTCTTCGGCGCTTTCGGCGAAGCTGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGc                                                                                                                                                                                                                                                >  1:173452/1‑300 (MQ=255)                                                                           aaGCTGAACAGTTTATNGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCNAAGTTGCGGGTTTTTTAANACGGAAAATGAATCAGc                                                                                                                                                                                                                   >  1:535684/1‑300 (MQ=255)                                                                                     gTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCACCGTCAt                                                                                                                                                                                                         >  1:76843/1‑300 (MQ=255)                                                                                     gTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGAACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCAt                                                                                                                                                                                                         >  2:391073/1‑300 (MQ=255)                                                                                     gTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAAAGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAGATGAATCAGCTCAACGTCAt                                                                                                                                                                                                         >  2:71722/1‑300 (MQ=255)                                                                                      tttATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCTGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTTTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGt                                                                                                                                                                                                            <  2:133474/296‑1 (MQ=255)                                                                                      tttATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGt                                                                                                                                                                                                            <  1:49280/296‑1 (MQ=255)                                                                                                  gCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACttt                                                                                                                                                                                            >  1:381366/1‑300 (MQ=255)                                                                                                             aaaCCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAAc                                                                                                                                                                                 <  1:372743/300‑1 (MQ=255)                                                                                                               aCCACTTCAACGTGGTGCGGGACATTCAGGATGTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAATTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCt                                                                                                                                                                               <  2:261607/300‑1 (MQ=255)                                                                                                                  aCTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTcc                                                                                                                                                                            >  1:210112/1‑300 (MQ=255)                                                                                                                   cTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATTAGCTCAACGTCGTCCGCAGTGACTTTCACCATTGAACCTTca                                                                                                                                                                            >  2:278283/1‑298 (MQ=255)                                                                                                                   cTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTcc                                                                                                                                                                            >  1:564499/1‑299 (MQ=255)                                                                                                                        aCGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGc                                                                                                                                                                      <  1:516116/300‑1 (MQ=255)                                                                                                                             gtgCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCGGTCGCTTTTGGACCCCATCACGATTGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATg                                                                                                                                                                       <  1:61544/294‑1 (MQ=255)                                                                                                                             gtgCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGc                                                                                                                                                                 >  1:60189/1‑300 (MQ=255)                                                                                                                             gtgCGGGACATTCAGGATTTCGAAGATTTAGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTACGTATg                                                                                                                                                                       >  2:61544/1‑294 (MQ=255)                                                                                                                                      aTTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTAc                                                                                                                                                        <  1:57473/300‑1 (MQ=255)                                                                                                                                       ttCAGGATTTCGAAGATTTCGGCTGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGNGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTAcc                                                                                                                                                       <  2:512710/300‑1 (MQ=255)                                                                                                                                                   aaGATCTCGGCGGCGAACTGCATGTTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCTGTAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGTGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCTTTGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAg                                                                                                                                             <  2:235610/298‑1 (MQ=255)                                                                                                                                                                    cTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGCAAAGCAGGTTGCTGATTGGCGAt                                                                                                                          >  1:395807/1‑300 (MQ=255)                                                                                                                                                                    cTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGAt                                                                                                                          >  2:448891/1‑300 (MQ=255)                                                                                                                                                                        aTGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGt                                                                                                                      <  2:577894/300‑1 (MQ=255)                                                                                                                                                                              gCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAGTCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCAttc                                                                                                                >  2:405668/1‑298 (MQ=255)                                                                                                                                                                                  aGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTNGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCAc                                                                                                              <  1:456024/298‑1 (MQ=255)                                                                                                                                                                                  aGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCAc                                                                                                              <  1:597584/298‑1 (MQ=255)                                                                                                                                                                                  aGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGAACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCAc                                                                                                              <  1:391073/298‑1 (MQ=255)                                                                                                                                                                                        ttttGGACCCCATCACGATGGCTACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCTGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGc                                                                                                      <  2:232585/300‑1 (MQ=255)                                                                                                                                                                                        ttttGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCTGTGACTTTTACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGc                                                                                                      <  1:85953/300‑1 (MQ=255)                                                                                                                                                                                        ttttGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGc                                                                                                      <  1:86271/300‑1 (MQ=255)                                                                                                                                                                                              aCCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATggg                                                                                                <  2:45226/300‑1 (MQ=255)                                                                                                                                                                                               ccccATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTCAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGt                                                                                               >  2:409854/1‑300 (MQ=255)                                                                                                                                                                                                    tCACGATGGCGACACGCGCCGGATTATTGCTGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGcc                                                                                          <  2:645434/300‑1 (MQ=255)                                                                                                                                                                                                      aCGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCg                                                                                        <  1:200989/300‑1 (MQ=255)                                                                                                                                                                                                           ggctacacGCGCCTGATTATTGCGGGAAGGCATGCGTCTTAAAACTCCTGTGGTGCACAACCCTCGTCTTTATAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGAt                                                                                   <  2:332511/296‑1 (MQ=255)                                                                                                                                                                                                              gACACGCGCCGGATTATGGCTGGAAGATATTCGTCTTAAAACTCCTGTGGTGCACAACTCGCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGTAAGGAAAACGGTTACGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCTTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAg                                                                                                                                             <  2:235596/239‑1 (MQ=255)                                                                                                                                                                                                                     gcCGGATTATTGCTGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGc                                                                         <  2:76843/300‑1 (MQ=255)                                                                                                                                                                                                                        ggATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGAGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCAcc                                                                      >  2:410522/1‑300 (MQ=255)                                                                                                                                                                                                                               tGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGNGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGctggtgggtaggcaagtgtagcggggggt                                                               <  1:467929/300‑29 (MQ=255)                                                                                                                                                                                                                                                 ttAAAACTCCTTTGGTGCACAACTCTCGGCTGTAGAGTGCACAGAGAATAGCACGAGAAGAGAGCAAGAAAAACGGTTGCGTGGCTGGGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAACAGGTTGCTGATTGTCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCATCCATTGCACCTGATGTTTTTCCATCGCACTGacc                                              <  2:268928/299‑1 (MQ=255)                                                                                                                                                                                                                                                 ttAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGacc                                              <  1:94929/299‑1 (MQ=255)                                                                                                                                                                                                                                                 ttAAAACTCCTGTGGTGCACAACTCTCGGCTTGAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTTTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGTCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGacc                                              <  2:14756/299‑1 (MQ=255)                                                                                                                                                                                                                                                     aaCTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCGGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTTATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGACCACcgc                                         <  2:587705/300‑1 (MQ=255)                                                                                                                                                                                                                                                            gtggtgCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGtttt                                    >  1:70293/1‑298 (MQ=255)                                                                                                                                                                                                                                                               gtgCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTa                               >  2:387032/1‑300 (MQ=255)                                                                                                                                                                                                                                                                             gCTTTAGAGGGCACCGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCAATGACCACCGCGTTTTGGTTAACGTCCATGATCGc                 >  2:424890/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                     gggCACAGAGAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTGTGGTTAACGTCCATGATCGCCAGCGAtt         >  2:352847/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                           agagAATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTActgc   <  1:240402/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                            gagaATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTActgct  >  1:100007/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                              gaATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTActg    >  1:375284/1‑296 (MQ=255)                                                                                                                                                                                                                                                                                              gaATAGCACGAAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCAGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTActg    >  1:175769/1‑296 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                TGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGCTGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCT  >  NC_000913/552866‑553448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑