Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,955,304	C→T	R609R (CGC→CGT)	ilvD →	dihydroxy‑acid dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,955,304	0	C	T	100.0%	179.2 / NA	57	R609R (CGC→CGT)	ilvD	dihydroxy‑acid dehydratase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/39);  total (18/39)

ATCACCGACGGTCGTTTCTCTGGTGGCACCTCTGGTCTTTCCATCGGCCACGTCTCACCGGAAGCGGCAAGCGGCGGCAGCATTGGCCTGATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCAGTGCACAGCTTTAAGCTGCGCGGCGCATACGCCATGATGGCGGGCCTGACGGAAGAACAGAAAGCGCACGGCGTGATCACTGCTTCTGCGGGTAACCA  >  NC_000913/3955008‑3955599                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                        aTCACCGACGGTCGTTTCTCTGGTGGCACCTCTGGTCTTTCCATCGGCCACGTCTCACCGGAAGCGGCAAGCGGCGGCAGCATTGGCCTGATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGGGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGAt                                                                                                                                                                                                                                                                                                      <  2:568211/300‑1 (MQ=255)                          gCACCTCTGGTCTTTCCATCTGCCACGGCTCACCTGAATCGGCAAGCGGCGGCAGCATTGGCCCGATGGAAGATGGCGACCTGATCGCTATCGACATCCCGAACCGTGGCATTCGGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGTTGACAAAGTCTGGACGCCGAGAAAGCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTtaataa                                                                                                                                                                                                                                                                              <  2:243842/299‑1 (MQ=255)                               tctGGTCTTTCCATCTGCCACGTCTCACCGGAAGCGGCAAGCTGCGGCAGCATTGGCCTGATTGGAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATGCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGTACGCCGAAAAATCGTGAACGTCAGTTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCTTGATAAAt                                                                                                                                                                                                                                                                                                  <  2:258846/274‑1 (MQ=255)                                  gttCTTTCCATCGGCCACGTCTCACCGGAAGCGGCAAGCGGCGGCAGCATTGGCCTGATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACt                                                                                                                                                                                                                                                                     <  2:623683/298‑1 (MQ=255)                                           aTCGGCCACGTCTCACCTGAAGCGGCAAGCGGCGGCAGCATTGGCCTGATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCNTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAAcccc                                                                                                                                                                                                                                                            <  2:210010/300‑1 (MQ=255)                                           aTCGGCCACGTCTCACCGGAAGCGGCAAGCGGCGGCAGCATTGGCCTGATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTTAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAAcccc                                                                                                                                                                                                                                                            <  1:34056/300‑1 (MQ=255)                                           aTCGGCCACGTCTCACCGGAAGCGGCAAGCGGCGGCAGCATTGGCCTGATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAAccc                                                                                                                                                                                                                                                             <  1:533237/299‑1 (MQ=255)                                              ggCCACGTCTAACCGGAAGCGGCAAGCGGCGGCAGCATTGGCCTGATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCNTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAAccc                                                                                                                                                                                                                                                             >  2:498414/1‑296 (MQ=255)                                                     tctcACCGGAAGCGGCAAGCGGCGGCAGCATTGGCCTGATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGc                                                                                                                                                                                                                                                  <  2:529599/300‑1 (MQ=255)                                                        cACCGGAAGCGGCAAGCGGCGGCAGCATTGGCCTGACTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCATCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCACCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGCCTCGCCACCCCTGTCCGGTGCTcc                                                                                                                                                                                                                                               >  2:526088/1‑300 (MQ=255)                                                            ggAAGCGGCAAGCGGCGGCAGCATTGGCCTGATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCTAGTTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGaa                                                                                                                                                                                                                                           <  2:47488/300‑1 (MQ=255)                                                              aagcggcaagcggcGGCAGCATTGGCCTGATTGAAGATGTTGACCTGATCGCTATCGACATCCCGAACCTTTGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCGGGACGCTCGAGGTGACAAAACCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCTGGag                                                                                                                                                                                                                                           <  2:186677/298‑2 (MQ=255)                                                                      agcgtcgtcagcaTTGGCCTGATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAATGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAat                                                                                                                                                                                                                                 <  2:26704/292‑1 (MQ=255)                                                                      agcggcGGCAGCATTGGCCTGATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAat                                                                                                                                                                                                                                 <  1:66976/300‑1 (MQ=255)                                                                                gcaTTGGCCTGATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGTTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCATCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGGGCTCCGGAAGGTGCCGAATATTTAAGAGc                                                                                                                                                                                                                       <  2:529201/300‑1 (MQ=255)                                                                                gcaTTGGCCTGATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGTTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGc                                                                                                                                                                                                                       <  2:245708/300‑1 (MQ=255)                                                                                gcaTTGGCCTGATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACTCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGc                                                                                                                                                                                                                       <  2:529190/300‑1 (MQ=255)                                                                                     ggCCTGATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCCGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGtgc                                                                                                                                                                                                                  <  2:453924/300‑1 (MQ=255)                                                                                          gATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCTTGACATTCAGTTACAGGTAAGCGATGCCGAACTTGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTgcgc                                                                                                                                                                                                             <  2:16332/300‑1 (MQ=255)                                                                                          gATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCTAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTgcgc                                                                                                                                                                                                             <  2:97432/300‑1 (MQ=255)                                                                                          gATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCCGCGCCGACAACGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCAGGAAGGTGCCGAATATTTAAGAGCAGtgctgc                                                                                                                                                                                                               >  2:342917/1‑298 (MQ=255)                                                                                          gATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTgcgc                                                                                                                                                                                                             <  1:644694/300‑1 (MQ=255)                                                                                          gATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCAGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCAAGCCTGGCACCCAGCGCCGACACAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACACCTGTACGGTGCTCCGGAAGGTGCCGAATATTTCAGAGCAGTGCTgcgc                                                                                                                                                                                                             >  2:103253/1‑300 (MQ=255)                                                                                                        aCCTGATCGCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGa                                                                                                                                                                                               <  1:585125/300‑1 (MQ=255)                                                                                                                          tCCCGAACCTTGGCATTCATTTACAGGCAAGCGAGGCCGAGCTGGCGGCGCTTCTGGAAGCGCAGGACGCTCGAGGCGACAAAGCCTGGACGCCGAAAAATCGTGAACATCAGATCGCCTTTCCCCTGCGTGCTGATGCCAGCCTGGCAACCAGCGCCGACAAAAGCGCGGTGCGTGATAAATCGAAACGGTGGGGTTAAGAACGGCTGACTCGTAACACCGGTCCGGTGCTCCTGGAGGTGCCGAATAGTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTAcgcc                                                                                                                                                                             <  2:304206/300‑1 (MQ=255)                                                                                                                          tCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTAcgcc                                                                                                                                                                             >  1:603255/1‑300 (MQ=255)                                                                                                                           cccGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGTTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTAcgcc                                                                                                                                                                             >  2:348110/1‑299 (MQ=255)                                                                                                                           cccGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCAGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTAcgc                                                                                                                                                                              >  2:641121/1‑298 (MQ=255)                                                                                                                           cccGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGACGGCGCGTCGTGAAGCGCCGGACGCTCAAGGTGGCAAAGCCTGGATGCAGACAAATCGTGAATGTCAGGTCTCCTTTGCCCTGCGTGTTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAACATGGGGGGTTACTAATGGCTGACTCGCACCCCCGGTCAGTTGCTCCGGAAGATGCCGCATGTTTAATAGCAATGCTGCGCACACCGGTTTCCGAGGCGGCGCAAGTTAcgcc                                                                                                                                                                             >  2:348117/1‑299 (MQ=255)                                                                                                                                   gTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACaaaa                                                                                                                                                                    >  1:440403/1‑300 (MQ=255)                                                                                                                                                                                     gcgcAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAgcgc                                                                                                                   >  1:66580/1‑299 (MQ=255)                                                                                                                                                                                         aGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGa                                                                                                              <  2:6171/300‑1 (MQ=255)                                                                                                                                                                                            aCGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATc                                                                                                            >  1:625425/1‑299 (MQ=255)                                                                                                                                                                                                   aGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACTTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCGACCAGCGCCGACAAAGGCGCGGTGCGTGATGAATCGAAACTGGGGGGTTAATAATGTCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTc                                                                                                                              <  2:430819/274‑1 (MQ=255)                                                                                                                                                                                                   aGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCgccagcca                                                                                                    >  2:519904/1‑300 (MQ=255)                                                                                                                                                                                                       gACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCAGTGc                                                                                                <  1:44482/300‑1 (MQ=255)                                                                                                                                                                                                            aGCCTGGACGCCGAAAAATCGTGAACGTCAGTTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAGCTGGGGGGTTAATAATTGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCAGTGCACAGc                                                                                           <  2:305450/300‑1 (MQ=255)                                                                                                                                                                                                            aGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAATTGTGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCAGTGCACAgt                                                                                           <  2:341655/300‑2 (MQ=255)                                                                                                                                                                                                            aGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCAGTGCACAGc                                                                                           <  1:364816/300‑1 (MQ=255)                                                                                                                                                                                                            aGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTAAAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCAGTGCACAGc                                                                                           >  1:304713/1‑300 (MQ=255)                                                                                                                                                                                                            aGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAAGAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCAGTGCACAGc                                                                                           <  1:364825/300‑1 (MQ=255)                                                                                                                                                                                                            aGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCATTTGCACTGCGTGCTTATGCCAGCCTGGCAACCAGTGCAGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTACGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCACAGGTTACGCCGCTAAAAAAAATGGAAAAACTGTAGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGAAAGACAGTGCAAAGc                                                                                           >  2:2106/1‑300 (MQ=255)                                                                                                                                                                                                                tGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCAGTGCACAGCtt                                                                                         <  1:122/298‑1 (MQ=255)                                                                                                                                                                                                                   aCGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGTGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCAGTGCACAGCTTTAAGc                                                                                    <  2:239030/300‑1 (MQ=255)                                                                                                                                                                                                                   aCGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCAGTGCACAGCTTTAAGc                                                                                    <  1:622557/300‑1 (MQ=255)                                                                                                                                                                                                                      ccGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCAGTGCACAGCTTTAAGCTgc                                                                                 >  1:371681/1‑300 (MQ=255)                                                                                                                                                                                                                        gAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCAGTGCACAGCTTTAAGCTgcgc                                                                               <  1:236240/300‑1 (MQ=255)                                                                                                                                                                                                                                               ccTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGTCGACGAAGGCGCGGTGCGTGATAAGTCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCATGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGTTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCAGCTACAAAAAATGGAAAAACTGTCGTCGAGTCTTGATAACGTAATTCTGGTGAAGCGCGAAGATCGCCGGCCAGTGCACAGCTTTAAGCTGCGCGGCGCCTACGCCGTGATGGc                                                           >  2:306071/1‑297 (MQ=255)                                                                                                                                                                                                                                                    gCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCAGTGCACAGCTTTAAGCTGCGCGGCGCATACGCCatgat                                                              >  2:380708/1‑289 (MQ=255)                                                                                                                                                                                                                                                    gCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCAGTGCACAGCTTTAAGCTGCGCGGCGCATACGCCatgat                                                              <  1:380708/289‑1 (MQ=255)                                                                                                                                                                                                                                                            tGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGTGTGGTTAATAATGTCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGTTGAAGCGCGAAGATCGCCAGCCAGTGCACAGCTTTAAGCTGCGCGGCGCATACGCCATGATGGCGGGCCTGACg                                                 <  2:274097/294‑1 (MQ=255)                                                                                                                                                                                                                                                             gCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCAGTGCACAGCTTTAAGCTGCGCGGCGCATACGCCATGATGGCGGGCCTGACGGAAGAAc                                          <  1:640585/300‑1 (MQ=255)                                                                                                                                                                                                                                                                           ggCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTTGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCAGTGCACAGCTTTAAGCTGCGCGGCGCATACGCCATAATGGCGGGCCTGACGGAAGAACAGAAAGCGCACGGc                            <  2:237163/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                   gcgcCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCAGTGCACAGCTTTAAGCTGCGCGGCGCATACGCCATGATGGCGGGCCTGACGGAAGAACAGAAAGCGCACGGCGTGATCAc                    <  1:45326/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                               gcgcGGTGCGTAATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCNGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCAGTGCACAGCTTTAAGCTGCGCGGCGCATACGCCATGATGGCGGGCCTGACGTAAGAACAGAAAGCGCACGGCGTGATCACTGCTTCTGCggg        <  1:470274/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                    gTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGATTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCGGTGCCCAGCTTTAAGCTGCGCGGCGCATACGCCATGATGGCGGGCCTGACGGAAGAACAGAAAGCGCACGGCGTGATTACTGCTTCTGCGGGTAAcc   >  2:296483/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                     tGCGTGATAAATCGAAACTGGGGGGTTAATAATTGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCAGTGCACAGCTTTAAGCTGCGCGGCGCATACGCCATGATGGCGGGCCTGACGGAAGAACAGAAAGCGCACGGCGTGATCACTGCTTCTGCGGGTAACCa  <  1:213835/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                        ATCACCGACGGTCGTTTCTCTGGTGGCACCTCTGGTCTTTCCATCGGCCACGTCTCACCGGAAGCGGCAAGCGGCGGCAGCATTGGCCTGATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCAGTGCACAGCTTTAAGCTGCGCGGCGCATACGCCATGATGGCGGGCCTGACGGAAGAACAGAAAGCGCACGGCGTGATCACTGCTTCTGCGGGTAACCA  >  NC_000913/3955008‑3955599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑