Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,629,921	C→T	E228K (GAG→AAG)	yhiI ←	putative membrane fusion protein YhiI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,629,921	0	C	T	100.0%	200.2 / NA	59	E228K (GAG→AAG)	yhiI	putative membrane fusion protein YhiI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/35);  total (24/35)

ATTCCAGATGCTGCTGGAGTAATTCCGGTGGGATACGCGCTTTGACGCGGAACATCAGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTCGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGCCCCTCGTTGGGCCAGTGAACGGGAACGCGTATGACGTTTTGCTACGG  >  NC_000913/3629627‑3630211                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                   atTCCAGATGCTGCTGGAGTAATTCCGGTGGGATACGCGCTTTGACGCGGAACATCAGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGACTTGGCaa                                                                                                                                                                                                                                                                                               >  2:344542/1‑300 (MQ=255) atTCCAGATGCTGCTGGAGTAATTCCGGTGGGATACGCGCTTTGACGCGGAACATCAGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCATGCCGCCAGCACTTCGCCTGGCTTGGc                                                                                                                                                                                                                                                                                                 >  2:591372/1‑298 (MQ=255)       gatactgctgGAGTAATTCCGGTTGGATACGCGCTTTGACGCGGAACATCAGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGAt                                                                                                                                                                                                                                                                                         <  1:56538/296‑1 (MQ=255)        aTGCTGCTGGAGTAATTCCGGTTGGATACGCGCTTTGACGCGGAACATCAGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGAt                                                                                                                                                                                                                                                                                         <  1:57422/299‑1 (MQ=255)         tgctgcTGGAGTAATTCCGGTGGGATACGCGCTTTGACGCGGAACATCAGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATAc                                                                                                                                                                                                                                                                                       <  1:117585/300‑1 (MQ=255)          gctgctGGAGGAATTCCGGTTGGATACGCGCTTTGACGCGGAACATCAGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTGTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATAc                                                                                                                                                                                                                                                                                       <  2:263865/299‑1 (MQ=255)                 gAGTAATTCCGGTGGGATACGCGCTTTGACGCGGAACATCAGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCAcgc                                                                                                                                                                                                                                                                                >  2:441052/1‑299 (MQ=255)                              gggATACGCGCTTTGACGCGGAACATCAGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCACAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGAAGTCGCTGAGATCGAACATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCATGCGTACGTCaagaga                                                                                                                                                                                                                                                                   >  2:125327/1‑294 (MQ=255)                                       gcTTTGACGCTGAACATCAGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAg                                                                                                                                                                                                                                                          <  2:437305/299‑1 (MQ=255)                                                  gAACATCAGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATc                                                                                                                                                                                                                                              <  1:479981/300‑1 (MQ=255)                                                      atcattctCAGCCGTTCACCGCTTGTTTCGACGGTTTTTGGCGTGAACTGGGCTACACTGTCGACACAACTGATGGTGGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCTCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCACCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGat                                                                                                                                                                                                                                           <  2:314038/292‑1 (MQ=255)                                                      aTCAGTTTCAGCCGTTCATCGCTGTTTTCGACGTTTTTTTGCGTGAACTGGGCGACACTGGCGACAAAACTGATGTTTGCAGGAATACGCAGATCTGGCTCTGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGat                                                                                                                                                                                                                                           <  2:312214/299‑1 (MQ=255)                                                      aTCAGTTTCAGCCGTTCATCACTGGTTTCGACGGTTTTTGGCTTGAACTGGGCGACACTGGCGACAAAGCTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGTGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTGGGCAGGAAGAAAGTCATATAGACGTCGCTTAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCATCCTTCACGCGGGGCTTTCAGTCCGCTGTCATCGat                                                                                                                                                                                                                                           <  2:312175/299‑1 (MQ=255)                                                         aGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGANGAAAGTCATATAGACGTCGCTGAGATCGACCANATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATc                                                                                                                                                                                                                                        <  2:481681/299‑1 (MQ=255)                                                         aGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCt                                                                                                                                                                                                                                       >  2:111753/1‑300 (MQ=255)                                                         aGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCt                                                                                                                                                                                                                                       >  1:557142/1‑300 (MQ=255)                                                         aGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTAGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCt                                                                                                                                                                                                                                       >  1:557298/1‑300 (MQ=255)                                                         aGTTTCAGCCGTTCATCGCTAGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGTTTCAACGCCCAGTTTCAGCGTGCCCGCATGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGAACATATTCAGCACCCGAACGCCCGCCGCCAGCACTTAGCATGGCTTGGCAACCCGATACTACACGCGTACGCCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCt                                                                                                                                                                                                                                       >  2:112017/1‑300 (MQ=255)                                                               aGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGc                                                                                                                                                                                                                                  <  1:367483/299‑1 (MQ=255)                                                                gCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCaa                                                                                                                                                                                                                                <  2:546576/300‑1 (MQ=255)                                                                          gCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTc                                                                                                                                                                                                                      <  1:367055/300‑1 (MQ=255)                                                                                      ggTTTTTGGCTTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGANCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGc                                                                                                                                                                                                          <  2:470975/300‑1 (MQ=255)                                                                                             ggCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCAGTTCAGTGGCTTGTGCCGCTTc                                                                                                                                                                                                    >  2:615173/1‑299 (MQ=255)                                                                                                       gggCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGttt                                                                                                                                                                                         >  2:417450/1‑300 (MQ=255)                                                                                                       gggCGACACTGGCGACAAAACTGAGAGTTGCGGGAATACGCAGAGCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGCTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCACATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACTCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCACCGATACCTACGGCaa                                                                                                                                                                                                                                <  2:228226/261‑1 (MQ=255)                                                                                                       gggCGAAACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTATTCGGTTGGCAGGAAGGAAGTCATATAGACGTCGCTGAGATCGACCATATTTAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTACACGCGTCCGTCAAGCGGGGCTTTCCGTTCGCTGTCATCGATATCTGCGGCAATGCGACGTTCAGTGGCTTGTGCAGCTTCGACGCGGGttt                                                                                                                                                                                         >  2:418072/1‑300 (MQ=255)                                                                                                            acacTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGcc                                                                                                                                                                                    <  1:412758/300‑1 (MQ=255)                                                                                                                       aaaaCTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGatat                                                                                                                                                                          <  1:591824/299‑1 (MQ=255)                                                                                                                        aaaCTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTGAGCACCCGACCGCCTGCCGCCAGCATTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGTGCCTGAATGATAtt                                                                                                                                                                         >  2:54426/1‑299 (MQ=255)                                                                                                                            tGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGgtg                                                                                                                                                                     <  1:201368/299‑1 (MQ=255)                                                                                                                            tGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGgtg                                                                                                                                                                     <  2:398804/299‑1 (MQ=255)                                                                                                                            tGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATNCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGgtg                                                                                                                                                                     <  2:210737/299‑1 (MQ=255)                                                                                                                                                            gCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGAt                                                                                                                                    >  1:498531/1‑300 (MQ=255)                                                                                                                                                                        aGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTc                                                                                                                        >  1:444261/1‑300 (MQ=255)                                                                                                                                                                                 tCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCNCGCGTCCGTCACGCGGGGCTTTAAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTcc                                                                                                               >  2:458615/1‑300 (MQ=255)                                                                                                                                                                                 tCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCNGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTcc                                                                                                               >  2:498510/1‑300 (MQ=255)                                                                                                                                                                                      gCCCAGTTTCAGCGTGCCCGCTTGTTCGGTTGTCAGGAAGAAAGTCATATAGACTTGGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCGGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAgcgc                                                                                                          <  1:321695/300‑1 (MQ=255)                                                                                                                                                                                      gCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAgcgc                                                                                                          <  1:344542/300‑1 (MQ=255)                                                                                                                                                                                           gTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGTCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTc                                                                                                     >  1:79651/1‑300 (MQ=255)                                                                                                                                                                                           gTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTc                                                                                                     >  1:481382/1‑300 (MQ=255)                                                                                                                                                                                            tctCAGCGTGCCCGCCTGTGCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCGTATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCATGCGTCCGCCACGCGGGGCTTTCAGTTCGCTGTCATCGACATCGGCGGCAATGCGCCATTCAGTTGCTTGTGCCACTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAGGCCGATACCTGAGCTTTCGCCGATTCCAgcgc                                                                                                          <  2:634724/292‑1 (MQ=255)                                                                                                                                                                                                   gTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGctc                                                                                             >  1:375962/1‑300 (MQ=255)                                                                                                                                                                                                            tGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCACTCTCAgcggc                                                                                     <  1:107225/299‑1 (MQ=255)                                                                                                                                                                                                                                aGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTg                                                                <  1:108417/300‑1 (MQ=255)                                                                                                                                                                                                                                                 ttagaTCTACCATATTCAGCACCCTACCGCCTGCCACCAGCACTTCGCCTGGCTTGTCAACCCTATACTGCACGCGCCCGTCACTCTGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCTTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATGTTGGTGCGTGCCGCTTCTATAGCCACTTTAGAAGCCGATATCTGAGCTTTCGCCGATTCCAGCGCAGTTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGcc                                                <  2:435398/297‑1 (MQ=255)                                                                                                                                                                                                                                                 tGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCTTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGcc                                                <  2:436715/299‑1 (MQ=255)                                                                                                                                                                                                                                                  gagaTCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGccc                                               >  1:447013/1‑299 (MQ=255)                                                                                                                                                                                                                                                  gagaTCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTAATCCAGCTGTTGCGCAGAAATAGcccc                                              >  1:447807/1‑300 (MQ=255)                                                                                                                                                                                                                                                         aCCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTCTAGCCGCTTTAGAAGCCGATACCTGAGCt                                                                                                                           >  2:223722/1‑216 (MQ=255)                                                                                                                                                                                                                                                         aCCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCt                                                                                                                           <  1:223722/216‑1 (MQ=255)                                                                                                                                                                                                                                                         aCCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGCACCTAGtt                                         >  2:387521/1‑298 (MQ=255)                                                                                                                                                                                                                                                            atttcgaacACCCTACCTCCTGCCACTAACACTGCGCCTGTCTTGGCAACCCAAGACTACGCGCGTACGTCACGCGTGGCTTTCAGTTCGCTGTCATCGATAGCTGCGGCGATTCGCCGTTCGGTGGCTTGTGCCGCTTCGACGCGGTTTTGCGCCTGAATGATGTTGGGGCGGGCCGCTTCCATAGCCGCTTTAGAAACCAATACCTGAGCTTTCTCCGATTCCAGCGCAGCTCGGGCGCTCGCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCACAGAAATAGCCCCTCGGTGGGcc                                    <  2:74714/292‑1 (MQ=255)                                                                                                                                                                                                                                                            atatTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGCCCCTCGTTGGGcc                                    <  1:223771/300‑1 (MQ=255)                                                                                                                                                                                                                                                                          aCCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGTCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGCCCCTCGTTGGGCCAGTGAACGGGaa                        <  1:363405/298‑1 (MQ=255)                                                                                                                                                                                                                                                                              ccTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGCCCCTCGTTGGGCCAGTGAACGGGAACGCGTa                  >  1:258851/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                tGCCGCCAGCACTCCGCCTTTCTTTGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGTTGTCATCGAGATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCGGAATGATATTGGTGCGTGCCGCTTCTATGGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCACTCGCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGCCCCGCGTTGGGcc                                    <  2:73317/280‑1 (MQ=255)                                                                                                                                                                                                                                                                                 gccgccAGCACTTCGCTTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCTGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCATTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAACTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGCCCCTCGTTGGGCCAGTGAACGGGAACGCGTATGa               <  2:66467/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                 gccgccAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGNTATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGCCCCTCGTTGGGCCAGTGAACGGGAACGCGTATGa               <  1:122076/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                               gCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGCCCCTCGTTGGGCCAGTGAACGGGAACGCGTATGACGTTTTGCTACgg  <  1:103466/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                   ATTCCAGATGCTGCTGGAGTAATTCCGGTGGGATACGCGCTTTGACGCGGAACATCAGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTCGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGCCCCTCGTTGGGCCAGTGAACGGGAACGCGTATGACGTTTTGCTACGG  >  NC_000913/3629627‑3630211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑