Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,825,328	G→A	S83S (TCC→TCT)	mltB ←	membrane‑bound lytic murein transglycosylase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,825,328	0	G	A	100.0%	218.3 / NA	63	S83S (TCC→TCT)	mltB	membrane‑bound lytic murein transglycosylase B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/29);  total (34/29)

GCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTACTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGGGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGC  >  NC_000913/2825036‑2825622                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                       gCGTTGCCAGCGCACCGAGGATGCGAGCTTTCCCCATCAAGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTACTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCAGTTCGGACCTGATGGCGGTTTCACCGATATGGTTGGTGTCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCATGAGAGAGaa                                                                                                                                                                                                                                                                                                 >  1:620033/1‑300 (MQ=255)          gcgcATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTACTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCa                                                                                                                                                                                                                                                                                        >  1:363937/1‑300 (MQ=255)                   ggATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTACTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGAc                                                                                                                                                                                                                                                                               >  2:550860/1‑300 (MQ=255)                           gTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTACTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGaaa                                                                                                                                                                                                                                                                       >  2:420121/1‑300 (MQ=255)                           gTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTACTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCAATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGaaa                                                                                                                                                                                                                                                                       >  2:420141/1‑300 (MQ=255)                            ttttCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTACTCCATACACCTNCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAAc                                                                                                                                                                                                                                                                      <  1:143514/300‑1 (MQ=255)                                       aCGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTACTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATTGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTc                                                                                                                                                                                                                                                           <  1:268796/300‑1 (MQ=255)                                                                  aTAATCCCGACGATAATTTCCTGCGGTACTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCTGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTggg                                                                                                                                                                                                                                  <  2:102644/298‑1 (MQ=255)                                                                                           gTACTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATcgccgc                                                                                                                                                                                                       >  1:46227/1‑300 (MQ=255)                                                                                             aCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGccc                                                                                                                                                                                                     >  1:415286/1‑300 (MQ=255)                                                                                               tCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCAt                                                                                                                                                                                                   >  1:298642/1‑300 (MQ=255)                                                                                               tCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTACGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGATTATCCATCAGCCGCAGTACCGAATCCAGATGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCAt                                                                                                                                                                                                   >  2:137316/1‑300 (MQ=255)                                                                                                ccATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATc                                                                                                                                                                                                  >  2:377615/1‑300 (MQ=255)                                                                                                ccATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATc                                                                                                                                                                                                  >  1:251659/1‑300 (MQ=255)                                                                                                       ccTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCATGAGAGCGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGACCTGCTGGGCCTTCGGGTTATTAGCGAAATCGCCGCCCCTCTGCATca                                                                                                                                                                                           >  2:65384/1‑300 (MQ=255)                                                                                                          gCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCAAGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACAt                                                                                                                                                                                        <  1:310641/300‑1 (MQ=255)                                                                                                          gCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGCTCTGCACTTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACAt                                                                                                                                                                                        <  2:15735/300‑1 (MQ=255)                                                                                                             aCGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTGCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCATACGCTTCGCCTGATAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACAt                                                                                                                                                                                        <  2:591620/297‑1 (MQ=255)                                                                                                               gcgcgATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGtgc                                                                                                                                                                                   <  2:575016/300‑1 (MQ=255)                                                                                                                  cgATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGACCATCTGCATCACATTGtgctgc                                                                                                                                                                                >  1:51135/1‑300 (MQ=255)                                                                                                                  cgATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCAGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGtgctgc                                                                                                                                                                                >  2:156924/1‑300 (MQ=255)                                                                                                                    aTTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGtgctgc                                                                                                                                                                                >  2:603425/1‑298 (MQ=255)                                                                                                                           gCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGc                                                                                                                                                                       <  2:437954/300‑1 (MQ=255)                                                                                                                              tCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGACTNAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGTTGCGGCTCAAGCa                                                                                                                                                                      >  2:207825/1‑298 (MQ=255)                                                                                                                                                           gTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCAGGTGGTCGt                                                                                                                                       >  1:56155/1‑300 (MQ=255)                                                                                                                                                           gTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATCAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGt                                                                                                                                       >  1:487151/1‑300 (MQ=255)                                                                                                                                                           gTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCAACGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGCTGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCAAATTGTACTGCGGCTCAAGCAGGAAGCCGCCAGATGGCGTTCCGGTGGTCGt                                                                                                                                       >  1:56254/1‑300 (MQ=255)                                                                                                                                                               tGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATc                                                                                                                                    <  1:197620/299‑1 (MQ=255)                                                                                                                                                                gCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGTAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAg                                                                                                                                  <  2:439183/300‑1 (MQ=255)                                                                                                                                                                  aCGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATcac                                                                                                                                                                                          <  1:422564/242‑1 (MQ=255)                                                                                                                                                                  aCGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATcac                                                                                                                                                                                          >  2:422564/1‑242 (MQ=255)                                                                                                                                                                        tCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTa                                                                                                                          <  1:525330/300‑1 (MQ=255)                                                                                                                                                                            gCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGtt                                                                                                                      <  1:324505/300‑1 (MQ=255)                                                                                                                                                                                  taaATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCt                                                                                                                <  1:446162/300‑1 (MQ=255)                                                                                                                                                                                        ttttGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGATAGAGAATTTGCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTtgctgc                                                                                                          <  1:615980/300‑1 (MQ=255)                                                                                                                                                                                            gCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACa                                                                                                      <  1:400363/300‑1 (MQ=255)                                                                                                                                                                                               aTAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAggc                                                                                                   <  1:413246/300‑1 (MQ=255)                                                                                                                                                                                                      aGCCATGCGCCTTTCGGACCTGATTGCGGTTTCACCGATGTGTTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTTAGAGAGAACTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGgcaag                                                                                             <  2:67524/299‑1 (MQ=255)                                                                                                                                                                                                       gCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGTCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGgcaagca                                                                                           >  2:22778/1‑300 (MQ=255)                                                                                                                                                                                                       gCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCAGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGgtaagca                                                                                           >  2:84746/1‑300 (MQ=255)                                                                                                                                                                                                                cGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGNCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACaaaaa                                                                                  >  1:140337/1‑300 (MQ=255)                                                                                                                                                                                                                 gTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACaaaaa                                                                                  >  1:4812/1‑299 (MQ=255)                                                                                                                                                                                                                 gTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCATGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCAGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACAaaaaaa                                                                                  >  1:3436/1‑299 (MQ=255)                                                                                                                                                                                                                                  gTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGNCCATCTGCATCACATTGTGCTGCGGCTCAAGCNGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACAt                                                                <  2:487151/300‑1 (MQ=255)                                                                                                                                                                                                                                  gTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACAt                                                                <  1:217601/300‑1 (MQ=255)                                                                                                                                                                                                                                    tccACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTACTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCATGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAAGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATaa                                                              <  2:620033/298‑1 (MQ=255)                                                                                                                                                                                                                                     tCACCGATGTGTTTGGTTCCTGTTTACCCAGCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGATAGAGAATTTCCTACAACGGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATGAGCGAAATCGCCACCCATCTGCATCACATTGTGCTGCGGCTCAAACAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAac                                                             <  2:43044/300‑1 (MQ=255)                                                                                                                                                                                                                                       aCCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAacga                                                           <  2:415286/300‑1 (MQ=255)                                                                                                                                                                                                                                       aCCGATGTGGTTGGTGCCTGGGTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGATAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCGGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAacga                                                           <  2:303533/300‑1 (MQ=255)                                                                                                                                                                                                                                          gATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTNTCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGc                                                        >  1:141253/1‑300 (MQ=255)                                                                                                                                                                                                                                          gATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCAGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGc                                                        >  2:557024/1‑300 (MQ=255)                                                                                                                                                                                                                                          gATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCATGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTNTCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGc                                                        >  1:141257/1‑300 (MQ=255)                                                                                                                                                                                                                                            tgtgGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCtt                                                      >  2:583431/1‑300 (MQ=255)                                                                                                                                                                                                                                             gtgGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCtt                                                      >  1:377264/1‑299 (MQ=255)                                                                                                                                                                                                                                                    gtGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGGGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGNTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTTCTTCTCCAGGCGGCAAGCCCCACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGaa                                                   >  2:209324/1‑295 (MQ=255)                                                                                                                                                                                                                                                      gCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGa                                              >  1:624255/1‑298 (MQ=255)                                                                                                                                                                                                                                                          ggTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTCGAACATGAGGGGTc                                        >  2:118337/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  aTCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGNTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAAc                                <  1:134437/300‑1 (MQ=255)                                                                                                                                                                                                                                                                  aTCAGCCGCAGTACCGAATCCAAACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGNAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTNTCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAAc                                <  2:479884/300‑1 (MQ=255)                                                                                                                                                                                                                                                                         gCAGTACCGAATCGAGACGCTTCGCCTGAGAGAGAATTTCTTGCAACTGCTGACGATCGAAACTGTGTTTGTTCACCATTTTTTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAAc                                <  2:442832/293‑1 (MQ=255)                                                                                                                                                                                                                                                                                          cGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATAGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTAt          >  2:368194/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                                gCCTGAGATAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAg   <  2:363937/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                                gCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGc  <  1:559847/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                       GCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTACTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCCAGACGCTTCGCCTGGGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAACATGAGGGGTCCATTTAACAGATTCAACCAGGGGCAAGTATGGTAAAGC  >  NC_000913/2825036‑2825622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑