Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,792,209	G→A	V313M (GTG→ATG)	gabD →	succinate‑semialdehyde dehydrogenase (NADP(+)) GabD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,792,209	0	G	A	100.0%	176.2 / NA	52	V313M (GTG→ATG)	gabD	succinate‑semialdehyde dehydrogenase (NADP(+)) GabD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/24);  total (28/24)

AACGAACTGACCAGTAACCCGCTGGTGCGCAAACTGTCGTTTACCGGTTCGACCGAAATTGGCCGCCAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAGTGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCTGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGAC  >  NC_000913/2791924‑2792499                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                                   aaCGAACTGACCAGTAACCCGCTGGTGCGCAAACTGTCGTTTACCGGTTCGACCGAAATTGGCCGCCAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGcac                                                                                                                                                                                                                                                                                      <  1:239490/300‑1 (MQ=255)           ccAGTAACCCGCTGGTGCGCAAACTGTCGTTTACCGGTTCGACCGAAATTGGCCGCCAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGAc                                                                                                                                                                                                                                                                             >  1:580816/1‑299 (MQ=255)           ccAGTAACCCGCTGGTGCGCAAACTGTCATTTACTGGTTCGACCGAAATTGGCCGCCAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTGACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGTAGGCAATGAGCAAACTGCACATCGGCGAc                                                                                                                                                                                                                                                                             >  2:309099/1‑299 (MQ=255)                     gCTGGTGCGCAAACTGTCGTTTACCGGTTCGACCGAAATTGGCCGCCAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGNTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGAt                                                                                                                                                                                                                                                                    <  2:499390/298‑1 (MQ=255)                     gCTGGTGCGCAAACTGTCGTTTACCGGTTCGACCGAAATTGGCCGCCAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGTTCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATa                                                                                                                                                                                                                                                                   <  2:578487/299‑1 (MQ=255)                        ggTGCGCAAACTGTCGTTTACCGGTTCGACCGAAATTTGCCGCCAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGGCGCTGGAGCTGGGCGGTAACGCGCCATTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTNGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGAt                                                                                                                                                                                                                                                                    <  2:469032/295‑1 (MQ=255)                        ggTGCGCAAACTGTCGTTTACCGGTTCGACCGAAATTGGCCGCCAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACgg                                                                                                                                                                                                                                                               <  1:370569/300‑1 (MQ=255)                               aaaCTGTCGTTTACCGGTTCGACCGAAATTGGCCGCCAGTTAATGGAACAGTGCGCGAAAGAAATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCAcc                                                                                                                                                                                                                                                        <  1:363346/300‑1 (MQ=255)                                                        aaaTTGGCCGCCAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAg                                                                                                                                                                                                                               <  2:583184/300‑1 (MQ=255)                                                        aaaTTGGCCGCCAGTGAGGGGAACAGTGCGCGAAAGGCATAAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCATTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAAGTCCGCAACGCCGGGCAAGCCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGGATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAg                                                                                                                                                                                                                               <  2:583176/300‑1 (MQ=255)                                                                       tAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGACGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCAACATCGGGCTGCTGATCGATGAAAAAGCGGTAGCAAAAGt                                                                                                                                                                                                                  >  2:67218/1‑298 (MQ=255)                                                                         aTGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGaa                                                                                                                                                                                                              >  1:206650/1‑300 (MQ=255)                                                                         aTGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCAACATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGaa                                                                                                                                                                                                              >  1:51858/1‑300 (MQ=255)                                                                                 gTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGAAAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGCTCGATGACAAAGCGGTCGCCAAAGTGGAAGAGCatat                                                                                                                                                                                                      >  2:346287/1‑300 (MQ=255)                                                                                     gcgAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGc                                                                                                                                                                                                   <  1:47893/299‑1 (MQ=255)                                                                                               tCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCAACATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATgcgc                                                                                                                                                                                           >  2:633205/1‑297 (MQ=255)                                                                                                 aaGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGgag                                                                                                                                                                                      <  1:260394/300‑1 (MQ=255)                                                                                                    aaaGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCNCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGaaa                                                                                                                                                                                   <  1:121160/300‑1 (MQ=255)                                                                                                    aaaGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAATAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGaaa                                                                                                                                                                                   <  1:209704/300‑1 (MQ=255)                                                                                                       gtgtCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGgc                                                                                                                                                                                >  1:533212/1‑300 (MQ=255)                                                                                                                     gggCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGttt                                                                                                                                                                   >  1:241425/1‑299 (MQ=255)                                                                                                                         ggTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCGGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCNATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTgcggc                                                                                                                                                              <  2:460208/300‑1 (MQ=255)                                                                                                                                       tATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGTGCAAACCTACGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAg                                                                                                                                                       <  2:363197/293‑1 (MQ=255)                                                                                                                                         tCGTCTTCGACGATGCCGGCCTCGACAAAGCCGTTGGAGGCGCGCTGTCCTCGAAATTCCGCAACGCCGGGCAAACCTGCATCTGCACTAACCACCTGTATGTGCAATACGGCGTGTATGACCGGTGTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGTTCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAATTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGTGGCGGTAAAg                                                                                                                                                       <  2:363210/291‑1 (MQ=255)                                                                                                                                                                      gCCGTGGAAGGCGCGCTGGCCTCGACATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATAGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCAtt                                                                                                                 >  2:451724/1‑300 (MQ=255)                                                                                                                                                                      gCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCAtt                                                                                                                 >  1:199343/1‑300 (MQ=255)                                                                                                                                                                      gCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCAtt                                                                                                                 >  2:429189/1‑300 (MQ=255)                                                                                                                                                                      gCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCAACATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGAcc                                                                                                                    >  2:311067/1‑297 (MQ=255)                                                                                                                                                                         gTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCt                                                                                                               <  2:110580/299‑1 (MQ=255)                                                                                                                                                                            gAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCGGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGNCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCtggtg                                                                                                           <  2:465601/300‑1 (MQ=255)                                                                                                                                                                               ggcacgcTTGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCNCTGATCGATGAAAAAGCGGTAGCAAAAGTGGANGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGAc                                                                                                        <  2:488337/296‑1 (MQ=255)                                                                                                                                                                                       ggCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCNATGAGCAAACTGCACATCGGCGACGGGCTGGANAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGCCGTTCCGGc                                                                                                >  2:494618/1‑300 (MQ=255)                                                                                                                                                                                                             gggCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAaagaa                                                                          >  1:516578/1‑300 (MQ=255)                                                                                                                                                                                                                                   aCCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGccc                                                    <  1:519222/300‑1 (MQ=255)                                                                                                                                                                                                                                         tGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCTACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCg                                                  >  1:52734/1‑296 (MQ=255)                                                                                                                                                                                                                                         tGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCg                                                  >  1:231752/1‑296 (MQ=255)                                                                                                                                                                                                                                          gTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCCACGTGTCGACAGAAGCGACGTTCGGCCCCCTCGCCCCGCTGtt                                             >  1:16800/1‑300 (MQ=255)                                                                                                                                                                                                                                              gtgCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAANAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCTGTTcc                                           >  2:464469/1‑298 (MQ=255)                                                                                                                                                                                                                                              gtgCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCACCTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCTGTTcc                                           >  1:648747/1‑298 (MQ=255)                                                                                                                                                                                                                                              gtgCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCTGTTCCGc                                         >  1:117800/1‑300 (MQ=255)                                                                                                                                                                                                                                              gtgCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCTGTTCCGc                                         >  2:610412/1‑300 (MQ=255)                                                                                                                                                                                                                                              gtgCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCACTCGCCCCGCTGTTCCGc                                         >  1:358299/1‑300 (MQ=255)                                                                                                                                                                                                                                              gtgCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCAACATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGTGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTACAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCTGTTcc                                           >  2:118281/1‑298 (MQ=255)                                                                                                                                                                                                                                              gtgCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCAACATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGTGTGGTTTGTGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCAGCTGTTctgc                                         >  2:610415/1‑297 (MQ=255)                                                                                                                                                                                                                                              gtgCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTACAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCCACGCCAAAGTGTCGAAAGGAGAGACGTTCGGCCCCCTCGCCCCGCTGTTcc                                           >  2:439828/1‑298 (MQ=255)                                                                                                                                                                                                                                              gtgCAGGACGGCGTGTATGAACGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGCTGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCAGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCTGTTCCGc                                         >  2:118336/1‑300 (MQ=255)                                                                                                                                                                                                                                                          gtgtATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCTGTTCCGCTTTAAAGATGaa                             >  1:83805/1‑300 (MQ=255)                                                                                                                                                                                                                                                                      tttGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCTGTTCCGCTTTAAAGATGAAGCTGATGTGAtt                 <  1:515958/300‑1 (MQ=255)                                                                                                                                                                                                                                                                               aaaTTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCTGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGcc        <  1:427924/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                    gcagcaGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCTGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGa   <  1:307164/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                    gcagcaGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCATCGCCCCGCTGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGa   <  1:576125/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                      agcagGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCTGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGac  <  1:369877/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                          ggcaaTGAGCAAACGGCACATCGGCGACGGGCGGGATAACGGCGTAACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCTGTTCCGCTTTAAAGATGAAGCTGATGTGATTgcgc             <  2:275496/284‑1 (MQ=255)                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                                   AACGAACTGACCAGTAACCCGCTGGTGCGCAAACTGTCGTTTACCGGTTCGACCGAAATTGGCCGCCAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAGTGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCTGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGAC  >  NC_000913/2791924‑2792499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑