Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_012971846,2480GT42.9% 48.9 / 12.1 56S93Y (TCC→TAC) ybiWglycyl radical protein
Reads supporting (aligned to +/- strand):  ref base G (14/18);  new base T (0/24);  total (14/42)
Fisher's exact test for biased strand distribution p-value = 9.53e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCGCGAGCGACGTTCCGCTACTTTCTCGCGCAGACCATCAAGCCCTTTTTCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAAGTCATATTGCCCTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCTCATCGGTAAACATGCCGTAGCAACGATCCTGTACCGTCTGACCGCGCCACCAAGGGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGGAGACGGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATCCTCGTA  >  NC_012971/845953‑846541
                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                     
gcgagatgtgtataagagacagttCTCGCGCAGACCATCAAGCCCTTTTTCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAAGTCATATTGCCCTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCTCATCGGTAAACATGCCGTAGCAACGATCCTGTACCGTCTGACCGCGCCACCAAGGGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGGAGAc                                                                                                                                                                                                                                                                                                   >  1:670763/23‑300 (MQ=255)
 cgcgAGCGACGTTCCGCTACTTTCTCGCGCAGACCATCAAGCCCTTTTTCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAAGTCATATTGCCCTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCTCATCGGTAAACATGCCGTAGCAACGATCCTGTACCGTCTGACCGCGCCACCAAGGGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGGAGAc                                                                                                                                                                                                                                                                                                   >  1:563049/1‑299 (MQ=255)
        gaCGTTCCGCTACTTTCTCGCGCAGACCATCAAGCCCTTTTTCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAAGTCATATTGCCCTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCTCATCGGTAAACATGCCGTAGCAACGATCCTGTACCGTCTGACCGCGCCACCAAGGGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGTAGACGGTatat                                                                                                                                                                                                                                                                                            <  1:267118/299‑1 (MQ=255)
                gCTACTTTCTCGCGCAGACCATCAAGCCCTTTTTCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAAGTCATATTGCCCTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCTCATCGGTAAACATGCCGTAGCAACGATCCTGTACCGTCTGACCGCGCCACCAAGGGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCATATCATCAATCTCTTTTTCGATCCAGGAGACGGTATATTCCGGGa                                                                                                                                                                                                                                                                                     <  2:462442/298‑1 (MQ=255)
                gCTACTTTCTCGCGCAGACCATCAAGCCCTTTTTCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAAGTCATATTGCCCTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCTCATCGGTAAACATGCCGTAGCAACGATCCTGTACCGTCTGACCGCGCCACCAAGGGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGTAGACGGTATATTCCGGGa                                                                                                                                                                                                                                                                                     <  2:355170/298‑1 (MQ=255)
                                gACCATCAAGCCCTTTTTCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAAGTCATATTGCCCTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCTCATCGGTAAACATGCCGTAGCAACGATCCTGTACCGTCTGACCGCGCCACCAAGGGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGGAGACGGTATATTCCGGGAAGATCGGCGCGGCGCGaa                                                                                                                                                                                                                                                                   >  1:386401/1‑300 (MQ=255)
                                                                         gCCAGGTGCGCATCGCCGGAAGTCATATTGCCCTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCTCATCGGTAAACATGCCGTAGCAACGATCCTGTACCGTCTGACCGCGCCACCAAGTGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGTAGACGGTATATTCCTGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCt                                                                                                                                                                                                                            <  2:36624/298‑1 (MQ=255)
                                                                                  gcATCGCCGGAAGTCATATTGCCCTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCTCATCGGTAAACATNCCGTAGCAACGATCCTGTACCGTCTGACCGCGCCACCAAGGGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGTAGACGGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGAt                                                                                                                                                                                                                  <  1:158250/299‑1 (MQ=255)
                                                                                  gcATCGCCGGAAGTCATATTGCCCTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCTCATCGGTAAACATGCCGTAGCAACGATCCTGTACCGTCTGACCGCGCCACCAAGTGCACACTTCATTCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGGAGACGGTATATTCCTGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGAt                                                                                                                                                                                                                  <  1:96533/299‑1 (MQ=255)
                                                                                  gcATCGCCGGAAGTCATATTGCCCTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCTCATCGGTAAACATGCCGTAGCAACGATCCTGTACCGTCTGACCGCGCCACCAAGGGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCATTAGACGGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGAt                                                                                                                                                                                                                  <  2:735266/299‑1 (MQ=255)
                                                                                  gcATCGCCGGAAGTCATATTGCCCTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCTCATCGGTAAACATGCCGTAGCAACGATCCTGTACCGTCTGACCGCGCCACCAAGGGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGTAGACGGTATATTCCTGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGAt                                                                                                                                                                                                                  <  1:96522/299‑1 (MQ=255)
                                                                                  gcATCGCCGGAAGTCATATTGCCCTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCTCATCGGTAAACATGCCGTAGCAACGATCCTGTACCGTCTGACCGCGCCACCAAGGGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGTAGACGGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGAt                                                                                                                                                                                                                  <  1:213836/299‑1 (MQ=255)
                                                                                  gcATCGCCGGAAGTCATATTGCCCTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCTCATCGGTAAACATGCCGTAGCAACGATCCTGTACCGTCTGACCGCGCCACCAAGGGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGGAGACGGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGAt                                                                                                                                                                                                                  <  1:585615/299‑1 (MQ=255)
                                                                                       gCCGGAAGTCATATTGCCCTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCTCATCGGTAAACATGCCGTNGCAACGATCCTGTACCGTCTGACCGCGCCACCAAGGGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGTAGACGGTATATTCCTGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAACAATTAACTCATCGTGCTTGATCCAGATAGTGc                                                                                                                                                                                                             <  1:549763/299‑1 (MQ=255)
                                                                                       gCCGGAAGTCATATTGCCCTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCTCATCGGTAAACATGCCGTNGCAACGATCCTGTACCGTCTGACCGCGCCACCAAGGGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGTAGACGGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGc                                                                                                                                                                                                             <  1:549624/299‑1 (MQ=255)
                                                                                       gCCGGAAGTCATATTGCCCTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCTCATCGGTAAACATGCCGTAGCAACGATCCTGTACCGTCTGACCGCGCCACCAAGGGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGTAGACGGTATATTCCTGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGc                                                                                                                                                                                                             <  2:105289/299‑1 (MQ=255)
                                                                                       gCCGGAAGTCATATTGCCCTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTCTGCTCATCGGTAAACATGCCGTNGCAACGATCCTGTACCGTCTGACCGCGCCACCAAGGGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGGAGACGGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGc                                                                                                                                                                                                             <  1:549549/299‑1 (MQ=255)
                                                                                            aaGTCATATTGCCCTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCTCATCGGTAAACATGCCGTAGCAACGATCCTGTACCGTCTGACCGCGCCACCAAGGGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGGAGACGGTATATTCCGGGAAGATTGGCGCGGCGCGAACTTCACTTGCCTGGTTGCCAATGATCAAATAACCGTGCTTGATCCAGATAGTGCGAtt                                                                                                                                                                                                         >  2:20037/1‑298 (MQ=255)
                                                                                            aaGTCATATTGCCCTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCTCATCGGTAAACATGCCGTAGCAACGATCCTGTACCGTCTGACCGCGCCAACAAGGGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGGAGACGNTATATTCAGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGCGATTc                                                                                                                                                                                                        >  2:595295/1‑299 (MQ=255)
                                                                                              gTCATATTGCCCTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCTCATCGGTAAACATGCCGTAACAACGATCCTGTACCGTCTGACCGCGCCACCAAAGGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGGAGACGGTATATTCAGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGCGATTcacc                                                                                                                                                                                                     >  2:195592/1‑297 (MQ=255)
                                                                                                 atatTGCCCTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCTCATCGGTAAACATGCCGTAGCAACGATTCTGTACCGTCTGACCGCGCCACCAAGGGCACACTTCATGCAGAACACGTTTGTTCTATTCGCTCACCGCAAAAACAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGGAGACGGTATATTCCGGGGAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGCGATTCGCCa                                                                                                                                                                                                    >  2:138483/1‑298 (MQ=255)
                                                                                                 atatTGCCCTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCTCATCGGTAAACATGCCGTAGCAACGATCCTGTACCGTCTGACCGCGCCACCAAGGGCACACTTAATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGTACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGGAGACGGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGCGCTTCGcaa                                                                                                                                                                                                    >  2:138474/1‑296 (MQ=255)
                                                                                                          tCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCTCATCAGTAAACATGCCGTAGCAACGATCCTTTACCGTCTGACCGCGCCACCAAGGGCACGCTTCATGCAGAACACGTGTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGGAGACGGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGCGATTCGCCAGGTGATgcg                                                                                                                                                                                           <  1:114947/298‑1 (MQ=255)
                                                                                                                                  agcagACCTTTTTGCTCATCGGTAAACATGCCGTAGCAACGATCCTGTACCGTCTGACCGCGCCACCAAGGGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGGAGACGGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCgg                                                                                                                                                                  <  2:733000/299‑1 (MQ=255)
                                                                                                                                   gcagACCTTTTTGCTCATCGGTAAACATGCCTTAGCAACGATCCTGTACCGTCTGACCGCGCCACCAAGTGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGTAGACGGTATATTCCTGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGa                                                                                                                                                                <  2:359200/300‑1 (MQ=255)
                                                                                                                                   gcagACCTTTTTGCTCATCGGTAAACATGCCGTAGCAACGATCCTGTACCGTCTGACCGCGCCACCAATGGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGGAGACGGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGa                                                                                                                                                                <  1:454282/300‑1 (MQ=255)
                                                                                                                                   gcagACCTTTTTGCTCATCGGTAAACATGCCGTAGCAACGATCCTGTACCGTCTGACCGCGCCACCAAGGGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGGAGACGGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGa                                                                                                                                                                <  1:408153/300‑1 (MQ=255)
                                                                                                                                          tttttGCTCATCGGTAAACATGCCGTAGCAACGATCCTGTACCGTCTGACCGCGCCACCAAGGGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGTAGACGGTATATTCCTGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGc                                                                                                                                                           <  1:575645/298‑1 (MQ=255)
                                                                                                                                                  cATCGGTAAACATGCCGTAGCAACGATCCTGTACCGTCTGACCGCGCCACCAAGGGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGGAGACGGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGAt                                                                                                                                                 >  1:85513/1‑300 (MQ=255)
                                                                                                                                                           aCATGCCTGAGCAACGATCCTGTACCGTCTGACCGCGCCACCAAGGGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGTAGACGGTATATTCCTGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGAt                                                                                                                                        <  2:376383/300‑1 (MQ=255)
                                                                                                                                                                  gTAGCAACGATCCTGTACCGTCTGACCGCGCCACCAAGTGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGGAGACGGTATATTCCGTGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACAtc                                                                                                                                   <  2:366848/298‑1 (MQ=255)
                                                                                                                                                                       aaCGATCCTGTACCGTCTGACCGCGCCACCAAGGGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGGAGACGGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGtat                                                                                                                            <  2:605272/300‑1 (MQ=255)
                                                                                                                                                                           aTCCTGTACCGTCTGACCGCGCCACCAAGGGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGGAGACGGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTAGGTATAGt                                                                                                                         >  1:458428/1‑299 (MQ=255)
                                                                                                                                                                            tCCTGTACCGTCTGACCGCGCCACCAAGGGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGTAGACGGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGCGATTCGCCAGTTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGtgc                                                                                                                       <  1:11247/300‑1 (MQ=255)
                                                                                                                                                                            tCCTGTACCGTCTGACCGCGCCACCAAGGGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGTAGACGGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCTAGATGTTGTTGATACATCTCGGTATAGtgc                                                                                                                       <  1:705868/300‑1 (MQ=255)
                                                                                                                                                                            tCCTGTACCGTCTGACCGCGCCACCAAGGGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGTAGACGGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGtgc                                                                                                                       <  1:705980/300‑1 (MQ=255)
                                                                                                                                                                            tCCTGTACCGTCTGACCGCGCCACCAAGGGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGGAGACGGTATATTCCTGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCTAGATGTTGTTGATACATCTCGGTATAGtgc                                                                                                                       <  1:68231/300‑1 (MQ=255)
                                                                                                                                                                            tCCTGTACCGTCTGACCGCGCCACCAAGGGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGGAGACGGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCTAGATGTTGTTGATACATCTCGGTATAGtgc                                                                                                                       <  1:375108/300‑1 (MQ=255)
                                                                                                                                                                            tCCTGTACCGTCTGACCGCGCCACCAAGGGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGGAGACGGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGtgc                                                                                                                       <  1:10756/300‑1 (MQ=255)
                                                                                                                                                                               tGTACCGTCTGACCGCGCCACCAAGGGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCATTAGACGGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGtgc                                                                                                                       <  1:12752/297‑1 (MQ=255)
                                                                                                                                                                                 tACCGTCTGACCGCGCCACCAAGGGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGTAGACGGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTgcgc                                                                                                                  <  2:10405/300‑1 (MQ=255)
                                                                                                                                                                                 tACCGTCTGACCGCGCCACCAAGGGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGGAGACGGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTgcgc                                                                                                                  <  1:395100/300‑1 (MQ=255)
                                                                                                                                                                                             gcgcCACCAAGGGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGTAGACGGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCTAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTAc                                                                                                       <  1:563972/299‑1 (MQ=255)
                                                                                                                                                                                             gcgcCACCAAGGGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGGAGACGGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTAc                                                                                                       <  1:563926/299‑1 (MQ=255)
                                                                                                                                                                                                  accaAGGGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGGAGACGGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCTAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACt                                                                                                  <  1:249887/299‑1 (MQ=255)
                                                                                                                                                                                                                       gAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGGAGACGGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCa                                                                            >  1:70137/1‑300 (MQ=255)
                                                                                                                                                                                                                                               ccGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGGAGACGGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATATTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGc                                                    >  1:252780/1‑300 (MQ=255)
                                                                                                                                                                                                                                                 gCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGTAGACGGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGc                                                    <  1:619845/298‑1 (MQ=255)
                                                                                                                                                                                                                                                             cccGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGGAGACGGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGCGATTCGCCAGGTCATGCGCCAGTGCCCGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCgt                                       >  1:281600/1‑299 (MQ=255)
                                                                                                                                                                                                                                                               cGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGGAGACGGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCg                                                                                                                                                             >  1:525357/1‑179 (MQ=255)
                                                                                                                                                                                                                                                               cGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGGAGACGGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCg                                                                                                                                                             <  2:525357/179‑1 (MQ=255)
                                                                                                                                                                                                                                                                        tGCCAGATCGTCAATCTCTTTTTCGATCCAGGAGACGGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTGGATCCAGATAGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAg                           <  1:138483/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                        tGCCAGATCATCAATCTCTTTTTCGATCCAGTAGACGGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAg                           <  1:138474/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                              atcatcAATCTCTTTTTCGATCCAGTAGACGGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCTAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGt                     <  2:652921/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                  tcaATCTCTTTTTCGATCCATTAGACGGTATATTCCTGGAAAATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATg                 <  2:461257/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                 aTCCAGTAGACGGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCTAGATGTTGTTGNTACATCTCGGTATAGTGCTGCGCGCGCTCGGTNCAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATCCTCGTa  <  2:563049/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                     
GCGCGAGCGACGTTCCGCTACTTTCTCGCGCAGACCATCAAGCCCTTTTTCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAAGTCATATTGCCCTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCTCATCGGTAAACATGCCGTAGCAACGATCCTGTACCGTCTGACCGCGCCACCAAGGGCACACTTCATGCAGAACACGTTTGTTCTCTTCGCTCACCGCAAAACCAGCACCCGGACGATCTGCCAGATCATCAATCTCTTTTTCGATCCAGGAGACGGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGCTTGATCCAGATAGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATCCTCGTA  >  NC_012971/845953‑846541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑