Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,304,522 2 bp→TC intergenic (+129/+585) eco → / ← mqo serine protease inhibitor ecotin/malate:quinone oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,304,5220GT93.3% 25.5 / NA 15intergenic (+129/+586)eco/mqoserine protease inhibitor ecotin/malate:quinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (5/9);  total (5/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009132,304,5230GC93.3% 30.1 / ‑4.9 15intergenic (+130/+585)eco/mqoserine protease inhibitor ecotin/malate:quinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (5/9);  minor base T (0/1);  total (5/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

GAAAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAG‑TACCGCGTCTGGAAGGCG‑GAAGAGA‑AAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGT‑CTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGT  >  NC_000913/2304237‑2304648
                                                                                                                                                                                                                                                                                                ||                                                                                                                              
gaaagaGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTTTATACGCCAGACAATGTAGATGTGAAG‑TACCGCGTCTGGAAGGCG‑GAAGAGA‑AAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTc                                                                                                                   <  2:476582/300‑1 (MQ=255)
     agaagaAATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAG‑TACCGCGTCTGGAAGGCG‑GAAGAGA‑AAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTcggata                                                                                                              <  1:631094/300‑6 (MQ=255)
                         tATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAG‑TACCGCGTCTGGAAGGCG‑GAAGAGA‑AAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCTGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGACCTACACCGCTGCGACGTGCTCCACCCCGTAGGTTGGATACGGCGGTTACGCCGCATcc                                                                                           >  1:843664/1‑299 (MQ=25)
                         tATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAG‑TACCGCGTCTGGAAGGCG‑GAAGAGA‑AAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATcc                                                                                           >  1:843524/1‑299 (MQ=255)
                                                gcGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAG‑TACCGCGTCTGGAAGGCG‑GAAGAGA‑AAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGtt                                                                    >  1:31633/1‑299 (MQ=255)
                                                                     gATCGTGGTGTATACGCCAGACAAGGTAGATGTGAAG‑TACCGCGTCTGGAAGGCG‑GAAGAGA‑AAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGt                                              <  2:712976/300‑1 (MQ=17)
                                                                             tgtATACGCCAGACAATGTAGCTGTGAAG‑TACCGCGTCTGGAAGGCG‑GAAGAGA‑AAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCTTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGTCCTACACCGCTTTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGT‑CTTATCAg                                     <  2:407899/300‑1 (MQ=17)
                                                                              gtATACGCCAGACAATGTAGATGTGAAG‑TACCGCGTCTGGAAGGCG‑GAAGAGA‑AAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGT‑CTTATCa                                      >  1:108577/1‑298 (MQ=255)
                                                                                   cGCCAGACAATGTAGATGTGAAG‑TACCGCGTCTGGAAGGCG‑GAAGAGA‑AAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGT‑CTTATCAGGCCt                                 >  1:270754/1‑298 (MQ=255)
                                                                                       aGACGATGTAGATGTGAAG‑TACCGCGTCTGGAAGGCG‑GAAGAGA‑AAATTGACACCGCGGTAGTTCGTTAAACTGCCGTGAAGTGTGGCAGCTAGTGGGTTAGATAAGGCGGTCATGTGGCATCTGATATCCAACGCCCTAGCTGGTTGCCTGATGCTACGCTGGCGTGTCTTATCGGGCCTACACCGCTGTGAAGTGCGTCACCCCGTAGGTCGGGTAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGTGT‑TTTATCAggtttacatt                            <  2:237580/299‑9 (MQ=12)
                                                                                          aatggTAGATGTGAAGNTACCGCGTCTGGAAGGCGNGAAGAGAGAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTNCTTATCAGGCCTacac                             <  1:700276/295‑1 (MQ=14)
                                                                                           acgctAGATGTGAAGNTACCGCGTCTGGAAGGCGNGAAGAGAGAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTNCTTATCAGGCCTacac                             <  1:700277/294‑1 (MQ=14)
                                                                                                   atgttaaggaccgcggtgggAAGGGG‑GAAGGTA‑AAATTGACAGCTCGTTAGTTCGCTAAACTGCCGTTAAATGCGGCACCCCTTAGGTCAGACACGTCGGTCACGCCGCATCCGACATCCAACGCCCGAGCTGGTTGCCGGATTCGACACTGTCGCTTCTGATCATGCCTACACCGCTTTTAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCCAACGCCCAAGCCGGTTGCCTGAGGCGACGCTGGCGCGT‑CTTATCAGGCCTACACCGCTGTGAAGTGc                <  2:463229/282‑1 (MQ=2)
                                                                                                                 gTCTGGAAGGCG‑GAAGAGA‑AAATTGACAACGCGGGAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGT‑CTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGt  <  1:415940/300‑1 (MQ=11)
                                                                                                                     ggAAGGCG‑GAAGAGA‑AAATCGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGTTCAGACAAGGCTGTCACGCCGCATCCGACAGCCAACGCCCGAACCGGTTGCCTGATGTGACACTGGCGCGTCGGATCATGCCTACACTGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGT‑CTTATCAg                                     <  2:409433/261‑1 (MQ=11)
                                                                                                                                                                                                                                                                                                ||                                                                                                                              
GAAAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAG‑TACCGCGTCTGGAAGGCG‑GAAGAGA‑AAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGT‑CTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGT  >  NC_000913/2304237‑2304648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑