Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,517,355	C→T	Q121Q (CAG→CAA)	fecR ←	ferric citrate regulator FecR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,517,355	0	C	T	100.0%	193.9 / NA	56	Q121Q (CAG→CAA)	fecR	ferric citrate regulator FecR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/30);  total (26/30)

ACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGT  >  NC_000913/4517061‑4517628                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                  aCCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCTAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGAc                                                                                                                                                                                                                                                                               <  1:690352/299‑1 (MQ=255) aCCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGAc                                                                                                                                                                                                                                                                               <  1:690341/299‑1 (MQ=255)             gATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGANGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGc                                                                                                                                                                                                                                                                   <  2:655404/299‑1 (MQ=255)                   tttttGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGTGCATCTTTCGCGGTAGTAATGTCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGgc                                                                                                                                                                                                                                                            <  1:191164/300‑1 (MQ=255)                   tttttGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCTCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGgc                                                                                                                                                                                                                                                            <  1:191579/300‑1 (MQ=255)                   tttttGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGgc                                                                                                                                                                                                                                                            <  1:625139/300‑1 (MQ=255)                                gCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGTCTGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGTCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATc                                                                                                                                                                                                                                                <  2:99427/299‑1 (MQ=255)                                           aaGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAANGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGAcc                                                                                                                                                                                                                                    <  1:186092/300‑1 (MQ=255)                                                cTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGAAACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCAGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTACCTTTGGCGGTGCGGTAATCTGCACGCAGACCTTCGc                                                                                                                                                                                                                               >  2:401828/1‑300 (MQ=255)                                                                    aCGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGc                                                                                                                                                                                                           >  2:827083/1‑300 (MQ=255)                                                                             tGCGTGAAATTATCCTGTTGGCGGACGGTAAATTCTGTTCCTAAAGCAGTGAGCTGGCCCTGGCGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGATTGCCAGAGCTGCCAGc                                                                                                                                                                                                    <  2:173931/298‑1 (MQ=255)                                                                              gCGTGAAATTATCCTGNTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGAGGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGANCTTCGCCGGTTTCCGACTNCCAGAGCTGCCAGCCTc                                                                                                                                                                                                 >  1:715996/1‑300 (MQ=255)                                                                                  gAAATTATCCTGCTGGCNGACGGTAAATTCTGTCCCNAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGNGCTGCCAGCCTCCGcc                                                                                                                                                                                             <  1:696216/300‑1 (MQ=255)                                                                                    aaTTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAg                                                                                                                                                                                           <  1:649931/300‑1 (MQ=255)                                                                                                   ggACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAAcagc                                                                                                                                                                             <  1:138456/299‑1 (MQ=255)                                                                                                                   cccTAAAGCAGTGAGCCGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTAGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCAAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTtcatcaa                                                                                                                                                            >  2:779695/1‑299 (MQ=255)                                                                                                                                    ggCCCTGACGGGTCAGGACACGGAAGGGGTGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGAGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTAATCAAGTGACGGAGGGTGAGGc                                                                                                                                           >  2:224777/1‑300 (MQ=255)                                                                                                                                    ggCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGAcggc                                                                                                                                                    <  1:334481/291‑1 (MQ=255)                                                                                                                                    ggCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGAAGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGAcggc                                                                                                                                                    >  2:334481/1‑291 (MQ=255)                                                                                                                                          gACGGGTCAGGACACGGNAGGGGCGTTGCAGGGCATNTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGNCGGGTGAGGCGGGTAt                                                                                                                                     <  1:696415/300‑1 (MQ=255)                                                                                                                                          gACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTAt                                                                                                                                     <  1:735897/300‑1 (MQ=255)                                                                                                                                          gACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGAGTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTAt                                                                                                                                     <  1:735651/300‑1 (MQ=255)                                                                                                                                              ggTCAGGACACGGAAGGGGCGTTGTAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCAGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGAAGGTTTCCAAATGACAGAGCTGCCAGCCTCCGACAGCGCAGAGCAACAGAAGTAATCCTTTAATCACGTGACGGCGGGTGGGGCGGGTATCGt                                                                                                                                  >  2:309579/1‑299 (MQ=255)                                                                                                                                                  aGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCaa                                                                                                                              <  1:271645/299‑1 (MQ=255)                                                                                                                                                   ggACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAAc                                                                                                                             >  1:303831/1‑299 (MQ=255)                                                                                                                                                         gggaGGGGCGTTGCAGGGCATCTTTCCCGGTAGTAATGTCGAGTTCGCCGTACCATAGCCGGACGTTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGTAAGCCATCTTCCAGTTGTTGACTGCTGACTGTGCCTTTGGCGGTGCAGTAATCTGCCCGCAGACCTTCGCTGGTTTCCGACTGCCGGAGCTGCCAGCTTTCGCCAGCGCCGAGCAATAGTAGTAATc                                                                                                                                                                      <  2:311547/249‑1 (MQ=255)                                                                                                                                                          gAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTNTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCNAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCt                                                                                                                     >  2:645920/1‑300 (MQ=255)                                                                                                                                                                   ttGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTc                                                                                                            >  1:138220/1‑300 (MQ=255)                                                                                                                                                                         ggCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTTGTAACGTCGCCa                                                                                                        >  1:121600/1‑298 (MQ=255)                                                                                                                                                                               tttCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGACCGGCTGGCAACGTCGCCAGGCACAcc                                                                                                >  2:136428/1‑300 (MQ=255)                                                                                                                                                                                    cgGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTTAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCaa                                                                                           >  1:761805/1‑300 (MQ=255)                                                                                                                                                                                    cgGTAGTAATGGCGATTTCACCGTACCAGAGCAGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTTAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGcc                                                                                             >  1:761796/1‑298 (MQ=255)                                                                                                                                                                                        agtaATGGCGATTTCACCGTACCAGAGCCGTACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTg                                                                                       <  2:715996/300‑1 (MQ=255)                                                                                                                                                                                                          gTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAAc                                                                     <  1:333389/300‑1 (MQ=255)                                                                                                                                                                                                           tACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGTGTAGTCAGCGTGAGCAGGTAGCCACCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTACCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACTTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCTGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAAcc                                                                    <  2:127072/300‑1 (MQ=255)                                                                                                                                                                                                           tACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCACGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACCGCAGTAATCCTTTCATCAAGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAAc                                                                     >  2:130562/1‑299 (MQ=255)                                                                                                                                                                                                           tACCAGAGCAGGACGGTGCGCTGATGCGCATCAGAACGCACGTCCGCCGCGCTTTGGTTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTGGTTTACGGCTGACTGTGCCTTTTGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTGTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACTTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCTGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCaccc                                                                    <  2:127049/300‑4 (MQ=255)                                                                                                                                                                                                           tACCAGAGCAGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTACGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCAAGTGACGGCGGGTGAGGCGGGTATCGTGCAACACCCGGCTGGCAACGTCGCCAGGCACACCGCAAAGCTGGTTGCGCAGGTTTTCaaac                                                                    >  2:420038/1‑298 (MQ=255)                                                                                                                                                                                                                gagCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACctgct                                                                >  2:744603/1‑299 (MQ=255)                                                                                                                                                                                                                      gACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATTTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTAGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGc                                                         >  2:349427/1‑300 (MQ=255)                                                                                                                                                                                                                      gACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTAATCACGTGACGGAGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCAAGGCACACCGCAAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCAAGGc                                                         >  2:349093/1‑300 (MQ=255)                                                                                                                                                                                                                          gTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCATAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCAc                                                     >  1:663524/1‑300 (MQ=255)                                                                                                                                                                                                                          gTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCAc                                                     >  1:30477/1‑300 (MQ=255)                                                                                                                                                                                                                          gTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGACAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCAc                                                     >  1:212492/1‑300 (MQ=255)                                                                                                                                                                                                                                tGATGCACATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGATTGTGCCTTTGGCGGGGCGGTAATCTACCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTgg                                                  <  2:28623/297‑1 (MQ=255)                                                                                                                                                                                                                                   tGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTatcc                                            <  1:742751/300‑1 (MQ=255)                                                                                                                                                                                                                                        aTCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTatcctgatc                                       <  2:831324/300‑1 (MQ=255)                                                                                                                                                                                                                                         tCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTatcctgatcc                                      >  1:804513/1‑300 (MQ=255)                                                                                                                                                                                                                                           aaaaCGCGCATCCGCCGCGCTTTGGTTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTTTCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTatcctaatcc                                      <  2:47539/298‑1 (MQ=255)                                                                                                                                                                                                                                              aCGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTTACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGtcc                                 <  1:315876/300‑3 (MQ=255)                                                                                                                                                                                                                                                    aTCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATAcc                            <  2:794035/299‑1 (MQ=255)                                                                                                                                                                                                                                                                          aGCGTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTc         >  1:137544/1‑296 (MQ=255)                                                                                                                                                                                                                                                                             gTGAGCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCtgttgt  <  1:589674/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                 gCAGGGAGCCATCTTCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCACGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTACtgttgt  >  2:177346/1‑296 (MQ=255)                                                                                                                                                                                                                                                                                              ttCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCGGCGCGCTTCCtgttg   >  2:47095/1‑282 (MQ=255)                                                                                                                                                                                                                                                                                              ttCCAGTTGTTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCtgttg   <  1:47095/282‑1 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                  ACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGT  >  NC_000913/4517061‑4517628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑