BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,342,825	C→T	N305N (AAC→AAT)	melA →	alpha‑galactosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,342,825	0	C	T	98.8%	258.8 / ‑3.9	85	N305N (AAC→AAT)	melA	alpha‑galactosidase
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (40/44); minor base G (1/0); total (41/44)
Fisher's exact test for biased strand distribution p-value = 4.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

GAGCTGGAGCGCAAAACCGCCGACGGCAGTTATGTGAATCTCTACCCGGAACTGCTGGCGGCTTATGAAGCAGGGCAGGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAACTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTCGGTACGCTACCTTCGCATCTGGCCGCCCTGATGCAAACCAACATCAACGTACAGACGCTGCTGACCGAAGC  >  NC_000913/4342535‑4343115

gagctggagcGCACAACCGCCGACGGCAGTTATGTGAATCTCTACCCGGAACTGCTGGCGGCTTATGAAGCAGGGCAGGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATaaa                                                                                                                                                                                                                                                                                             >  2:431141/1‑300 (MQ=255)

gagctggagcGCAAAACCGCCGACGGCAGTTATGTGAATCTCTACCCGGAACTGCTGGCGGCTTATGAAGCAGGGCAGGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGNATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGCGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATaaa                                                                                                                                                                                                                                                                                             >  2:267898/1‑300 (MQ=255)

 agctggagcGCAAAACCGCCGACGGCAGTTATGTGAATCTCTACCCGGAACTGCTGGCGGCTTATGAAGCAGGGCAGGNACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCTAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAag                                                                                                                                                                                                                                                                                            <  1:168495/300‑1 (MQ=255)

 agctggagcGCAAAACCGCCGACGGCAGTTATGTGAATCTCTACCCGGAACTGCTGGCGGCTTATGAAGCAGGGCAGGNACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAag                                                                                                                                                                                                                                                                                            <  1:166306/300‑1 (MQ=255)

          gcAAAACCGCCGACGGCAGTTATGTGAATCTCTACCCGGAACTGCTTGCGGCTTATGAAGCAGGGCAGGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCTTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTggagg                                                                                                                                                                                                                                                                                   <  2:174660/300‑1 (MQ=255)

                  gccgACGGCAGTTATGTGAATCTCTACCCGGAACTGCTGGCGGCTTATGAAGCAGGGCATGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGTCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATaa                                                                                                                                                                                                                                                                            <  2:14947/299‑1 (MQ=255)

                  gccgACGGCAGTTATGTGAATCTCTACCCGGAACTGCTGGCGGCTTATGAAGCAGGGCAGGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATaa                                                                                                                                                                                                                                                                            <  2:15017/299‑1 (MQ=255)

                     gACGGCAGTTATGTGAATCTCTACCCGGAACTGCTGGCGGCTTATGAAGCAGGGCAGGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTCAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTAGAGGAGTATaaaaaa                                                                                                                                                                                                                                                                        >  1:83795/1‑300 (MQ=255)

                     gACGGCAGTTATGTGAATCTCTACCCGGAACTGCTGGCGGCTTATGAAGCAGGGCAGGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATaaaaa                                                                                                                                                                                                                                                                         <  2:186290/299‑1 (MQ=255)

                      acgtcAGTTATGTGAATCTCTACCCGGAACTGCTGGCGGCTTATGAAGCAGGGCAGGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGTAGGAGTATaaaaa                                                                                                                                                                                                                                                                         <  2:192183/294‑1 (MQ=255)

                                      tctcTACCCGGAACTGCTGGCGGCTTATGAAGCAGGGCAGGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACTAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGTAGGAGTATAAAAAAGCCTCCCGGATTGatat                                                                                                                                                                                                                                                       <  2:723750/300‑1 (MQ=255)

                                      tctcTACCCGGAACTGCTGGCGGCTTATGAAGCAGGGCAGGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGatat                                                                                                                                                                                                                                                       >  1:782940/1‑300 (MQ=255)

                                       ctctACCCGGAACTGCTGGCGGCTTATGAAGCAGGGCAGGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCTTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATAtt                                                                                                                                                                                                                                                      >  1:491889/1‑300 (MQ=255)

                                           aCCCGGAACTGCTGGCGGCTTATGAAGCAGGGCAGGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAAc                                                                                                                                                                                                                                                  >  2:799351/1‑300 (MQ=255)

                                           aCCCGGAACTGCTGGCGGCTTATGAAGCAGGGCAGGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAAc                                                                                                                                                                                                                                                  >  1:538239/1‑300 (MQ=255)

                                           aCCCAGAACTGCTGGCGGCTTATGAAGCAGGGCAGGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAANGCTGGGCTATTTCGTCACGGAATCGTCAGAACNTTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGTGAATTGGCATAAAGGGCTGGAGGAGTATAAAAAAGCCTCCAGGATTGATATTAAAc                                                                                                                                                                                                                                                  >  2:627849/1‑300 (MQ=255)

                                                            gcTTATGAAGCAGGGCAGGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGcc                                                                                                                                                                                                                                 <  1:397322/300‑1 (MQ=255)

                                                              ttATGAAGCAGGGCAGGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCAAGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGGTTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAATGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAAACGTCACGGGAATATGCCa                                                                                                                                                                                                                                >  2:367776/1‑299 (MQ=255)

                                                                            aggcaCCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAAc                                                                                                                                                                                                                  <  1:558768/299‑1 (MQ=255)

                                                                                         gAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACt                                                                                                                                                                                                      <  2:308778/298‑1 (MQ=255)

                                                                                           atatTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTACAGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGc                                                                                                                                                                                                   >  1:237182/1‑299 (MQ=255)

                                                                                                  tGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGa                                                                                                                                                                                            >  1:74408/1‑299 (MQ=255)

                                                                                                  tGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTAACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCATCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGa                                                                                                                                                                                            >  1:74033/1‑299 (MQ=255)

                                                                                                    gCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGNAGTATAAAAAAGCCTCCAGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAgt                                                                                                                                                                                          >  2:653066/1‑299 (MQ=255)

                                                                                                    gCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCAGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAgt                                                                                                                                                                                          >  1:307187/1‑299 (MQ=255)

                                                                                                    gCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTACCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAgt                                                                                                                                                                                          >  1:338682/1‑299 (MQ=255)

                                                                                                       aTACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGa                                                                                                                                                                                      >  1:690892/1‑300 (MQ=255)

                                                                                                              cTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTAc                                                                                                                                                                                 >  1:479149/1‑298 (MQ=255)

                                                                                                                gCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCAGAGTGTGATTTACGGCa                                                                                                                                                                             >  2:535649/1‑300 (MQ=255)

                                                                                                                   aGAATCTTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGGTATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTCTCTGGACTGGCGAGCCGAGTGTGATTTa                                                                                                                                                                                  >  2:77002/1‑292 (MQ=255)

                                                                                                                    gAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGt                                                                                                                                                                         >  1:657267/1‑300 (MQ=255)

                                                                                                                      atatTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTcc                                                                                                                                                                       >  1:660983/1‑300 (MQ=255)

                                                                                                                                  aCGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGNGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGtt                                                                                                                                                          <  2:657267/300‑1 (MQ=255)

                                                                                                                                             aaaaaGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGAGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACTGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AAc                                                                                                                                              <  1:56099/300‑1 (MQ=255)

                                                                                                                                                         tatctCGTCACGGAATNGTCAGAACATTTTGCTGANGACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTTTGATTNAT‑AACCTGCCACAAgg                                                                                                                                   <  1:705209/295‑1 (MQ=255)

                                                                                                                                                          aTTTCGTAACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGNCGAATTGGCATAAAGAGCTGGAGGAGTATAAAGAAGCCTCCCGGATTGATATTAAACCGTCACGTGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAgg                                                                                                                                   <  2:277478/298‑1 (MQ=255)

                                                                                                                                                              cGTCACGGAATCGTCANAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAAGGTCCGTCNACGATGGTTTGATTGACCNACCTGCCACAAGGaa                                                                                                                                 >  1:698766/1‑297 (MQ=255)

                                                                                                                                                              cGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGc                                                                                                                                                                              >  1:283141/1‑253 (MQ=255)

                                                                                                                                                                                  aTTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGc                                                                                                                 >  1:97021/1‑292 (MQ=255)

                                                                                                                                                                                   ttttGCTGAGTACACACCTTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGt                                                                                                        <  1:278704/300‑1 (MQ=255)

                                                                                                                                                                                   ttttGCTGAGTACACACCGTTTTTTATTAAGCCAGGTCGTTAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACTGGAATATGCCAGCACAATCATGAACGCTATCTGGACTTGCGAGCCGAGTGTGATTTACGGCAATGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGt                                                                                                        <  2:774579/300‑1 (MQ=255)

                                                                                                                                                                                   ttttGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGt                                                                                                        <  1:278621/300‑1 (MQ=255)

                                                                                                                                                                                     ttGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGtt                                                                                                       >  1:515572/1‑299 (MQ=255)

                                                                                                                                                                                          gAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCt                                                                                                 >  2:160627/1‑300 (MQ=255)

                                                                                                                                                                                           aGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTa                                                                                                >  2:761501/1‑300 (MQ=255)

                                                                                                                                                                                            gTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCNTCCCGGATTGATATTAAACCGTCAAGGGAATATGCCAGCACACTCATGAACGCTATCTGGACTGGAGAGCCGAGTGTGATTTACGGCAAAGTCTGT‑AAAGATGGTTTGATTGAT‑AACCTGCCACCAAGATGTTACGTGGACGTAGCCTGTCTGGtt                                                                                                       >  2:609293/1‑292 (MQ=255)

                                                                                                                                                                                            gTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTaa                                                                                               >  1:545074/1‑300 (MQ=255)

                                                                                                                                                                                            gTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AAACTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTaa                                                                                               >  1:693738/1‑300 (MQ=255)

                                                                                                                                                                                            gTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AAAGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTaa                                                                                               >  2:483844/1‑300 (MQ=255)

                                                                                                                                                                                            gTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAAGTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGACACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTaa                                                                                               >  2:741072/1‑300 (MQ=255)

                                                                                                                                                                                              acacacCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATg                                                                                             <  1:321253/300‑1 (MQ=255)

                                                                                                                                                                                                   ccGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGCGCGTTATACAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCACGGATTGATATTAAACCGTCAAGGGAATATGCCAGCACAATCATGAACGATATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAAAGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCATGTCTGGCTGATGCTa                                                                                                >  2:201869/1‑292 (MQ=255)

                                                                                                                                                                                                     gTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCAtt                                                                                        <  1:723825/298‑1 (MQ=255)

                                                                                                                                                                                                        gTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGc                                                                                    <  1:796232/299‑1 (MQ=255)

                                                                                                                                                                                                          ttattaAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGTGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGACGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGa                                                                                 >  2:148491/1‑300 (MQ=255)

                                                                                                                                                                                                                    aGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTc                                                                         <  2:58049/298‑1 (MQ=255)

                                                                                                                                                                                                                     gggcGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCCCCCGGATTGATATTAAACCGTCACGGGAATATGCCAACACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTTTGATTGAC‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAATTCgg                                                                       <  2:375647/296‑1 (MQ=255)

                                                                                                                                                                                                                      ggcgttaGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCTAAACGCGGCTTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGTAGGAGTATAAAAAAGCCCCCCGGATTGATATTAAACCGTCACTGGAATATGCCAGCACAATCATGAACGCTACCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTc                                                                         <  2:83795/290‑1 (MQ=255)

                                                                                                                                                                                                                       tCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTCGGTAc                                                                    <  1:799351/300‑1 (MQ=255)

                                                                                                                                                                                                                            agggtttGATTGAGCGCTATAAAGTACCGCGGGATGAGTACCCGAAACGCTGCGTCGAGCAGTTGGCGAATTGGCATAAAGATCTGTAGGAGTAGAGAAAAGCCTCCCGGGTTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGGGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTCGGTACGCTAc                                                               <  2:108223/296‑1 (MQ=255)

                                                                                                                                                                                                                            aGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGNCTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTCGGTACGCTAc                                                               <  2:608769/300‑1 (MQ=255)

                                                                                                                                                                                                                             ggATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTCGGTACGCTAc                                                               >  2:193159/1‑299 (MQ=255)

                                                                                                                                                                                                                               aTTTGATTGAGCGTGATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCAGAAAGAGCGGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGTGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTCGGTACGCTACCtt                                                            <  2:347385/300‑1 (MQ=255)

                                                                                                                                                                                                                                     ttgaGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTCGGTACGCTACCTTCGCATc                                                      <  1:690417/300‑1 (MQ=255)

                                                                                                                                                                                                                                            ttATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTCGGTACGCTACCTTCGCATCTGgccgc                                               >  1:635206/1‑300 (MQ=255)

                                                                                                                                                                                                                                              ataAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTCGGTACGCTACCTTCGCATCTGGCCGccc                                             >  1:330326/1‑300 (MQ=255)

                                                                                                                                                                                                                                              ataAAGTACCGCTGGAGGAGTACCCTAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCTGATTGATATTAAACCGTCACTGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCTT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTCGGTACGCTACCTTCGCATCTGGCCGccc                                             <  2:102578/300‑1 (MQ=255)

                                                                                                                                                                                                                                                        gCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCTAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTCGGTACGCTACCTTCGCATCGGGCCGCCCTGATGCAAAc                                   <  2:789220/300‑1 (MQ=255)

                                                                                                                                                                                                                                                        gCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGTAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACACTCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACTGCAACGTCCGT‑AACGATGTTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTCGGTACGCTACCTTCGCATCTGGCCGCCCTGATGCAAAc                                   <  2:789228/300‑1 (MQ=255)

                                                                                                                                                                                                                                                           ggATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTCGGTACGCTACCTTCGCATCTGGCCGCCCTGATGCAAACCaa                                >  1:90336/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                 gTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTCGGTACGCTACCTTCGCATCTGGCCGCCCTGATGCAAACCAACATCaa                          >  1:785409/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                   aCCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTCGGTACGCTACCTTCGCATCTGGCCGCCCTGATGCAAACCAACATCAACg                        <  1:193159/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                        aaaCGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTCGGTACGCTACCTTCGCATCTGGCCGCCCTGATGCAAACCAACATCAACGTACAg                   <  1:267898/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                         aaCGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGTAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTCGGTACGCTACCTTCGCATCTGGCCGCCCTGATGCAAACCAACATCAACGTACAGa                  <  1:585222/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                         aaCGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTCGGTACGCTACCTTCGCATCTGGCCGCCCTGATGCAAACCAACATCAACGTACAGa                  <  2:844894/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                         aaCGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTCGGTACGCTACCTTCGCATCTGGCCGCCCTGATGCAAACCAACATCAACGTACAGa                  <  1:215870/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                         aaCGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTGTGATTGAT‑AACCTGCCACAAGGATGGTGCGTGGAAGGAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTCGGTACGCTACCTTCGCATCTGGCCGCCCTGATGCAAATCAACATCAACGTACAGa                  <  1:627849/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                         aaCGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTAAGGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTCGGTACGCTACCTTCGCATCTGGCCGCCCTGATGCAAACCAACATCAACGTACAGa                  <  1:143390/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                         aaCGCTGCGTCGAGCAGCTGGCGAATGGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCCCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTGACGGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTGGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTCGGTACGCTACCTGCGCATCTGGCCGCCCTGATGCAAACCAACATCAACGTACAGa                  <  2:785732/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                          aCGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTCGGTACGCTACCTTCGCATCTGGCCGCCCTGATGCAAACCAACATCAACGTACAGAc                 <  1:761501/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                            gCTGCGTCGAGCAGCTGGCTAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATGTGCCAGCACAATCATGGACGCTATCTGGACTGTCGAGCCGAGTGTGATTTACTGCAACGTCCGT‑AACGATGGTTTGGTTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTCGGTACGCTACCTTCGCATCTGGCCGCCCTGATGCAAACCAACATTAACGt                       <  2:165355/292‑1 (MQ=255)

                                                                                                                                                                                                                                                                            gCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAAAGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTCGGTACGCTACCTTCGCATCTGGCCGCCCTGATGCAAACCAACATCAACGTACAGACg                <  1:40716/299‑1 (MQ=255)

                                                                                                                                                                                                                                                                               gCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTCGGTACGCTACCTTCGCATCTGGCCGCCCTGATGCAAACCAACATCAACGTACAGACgctgc            >  1:101035/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                                      gcagcTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTCGGTACGCTACCTTCGCATCTGGCCGCCCTGATGCAAACCAACATCAACGTACAGACGCTGCTGACCGa     <  2:338682/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                                         gcTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTCGGTACGCTACCTTCGCATCTGGCCGCCCTGATGCAAACCAACATCAACGTACAGACGCTGCTGACCGAAGc  >  1:339409/1‑300 (MQ=255)

GAGCTGGAGCGCAAAACCGCCGACGGCAGTTATGTGAATCTCTACCCGGAACTGCTGGCGGCTTATGAAGCAGGGCAGGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAACTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGT‑AACGATGGTTTGATTGAT‑AACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTCGGTACGCTACCTTCGCATCTGGCCGCCCTGATGCAAACCAACATCAACGTACAGACGCTGCTGACCGAAGC  >  NC_000913/4342535‑4343115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`