Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,322,605	C→T	intergenic (‑183/+731)	phnF ← / ← phnD	putative transcriptional regulator PhnF/phosphonate/phosphate ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,322,605	0	C	T	100.0%	188.7 / NA	56	intergenic (‑183/+731)	phnF/phnD	putative transcriptional regulator PhnF/phosphonate/phosphate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/29);  total (27/29)

GCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTCGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAACAGCATGGCGAAGACCATTTCGTTAATGGCGCGGCAGGC  >  NC_000913/4322333‑4322894                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                  gCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCNTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGa                                                                                                                                                                                                                                                                        >  1:615519/1‑300 (MQ=255)  cGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGAt                                                                                                                                                                                                                                                                       >  1:638027/1‑300 (MQ=255)       gtgtTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGATAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAgg                                                                                                                                                                                                                                                                  <  2:235907/300‑1 (MQ=255)       gtgtTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATaga                                                                                                                                                                                                                                                                    >  1:112429/1‑298 (MQ=255)                aaacTCCTGCTCAAGTTTTGCTGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATNCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGgag                                                                                                                                                                                                                                                         <  2:261118/297‑1 (MQ=255)                aaGCTCCTGCTCAAGTTTTGCGGCTATTTCTTGATAGCGGGTTGGGTAGCTGGTCGGATGTGTAGACAAATGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGATAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGgag                                                                                                                                                                                                                                                         <  2:297382/300‑1 (MQ=255)                       tGCTCAATTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATcagc                                                                                                                                                                                                                                                   <  1:123088/299‑1 (MQ=255)                       tGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGTTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTAGCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGATGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATcagc                                                                                                                                                                                                                                                   <  1:110157/299‑1 (MQ=255)                       tGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATcagc                                                                                                                                                                                                                                                   <  1:123964/299‑1 (MQ=255)                        gCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATcagcag                                                                                                                                                                                                                                                 <  1:307008/300‑1 (MQ=255)                          tCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGGCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCCTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGTTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATcagc                                                                                                                                                                                                                                                   <  2:16497/296‑1 (MQ=255)                                   gCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCNGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCt                                                                                                                                                                                                                                      <  1:609447/300‑1 (MQ=255)                                          tctcTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGgca                                                                                                                                                                                                                                >  1:774589/1‑299 (MQ=255)                                             ctTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCAc                                                                                                                                                                                                                            >  2:320845/1‑300 (MQ=255)                                                      gtgtTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCAACTGTGGCAGCACGCCGTaga                                                                                                                                                                                                                    >  1:83452/1‑299 (MQ=255)                                                       tgtTTGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAgg                                                                                                                                                                                                                  <  2:571765/300‑1 (MQ=255)                                                       tgtTGGTTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACNGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAgg                                                                                                                                                                                                                  <  1:601479/300‑1 (MQ=255)                                                       tgtTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACNGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAgg                                                                                                                                                                                                                  <  1:600459/300‑1 (MQ=255)                                                       tgtTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGTGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCTACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATATAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAgg                                                                                                                                                                                                                  <  2:234339/300‑1 (MQ=255)                                                         ttGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGAt                                                                                                                                                                                                                <  1:768644/300‑1 (MQ=255)                                                                        gATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGATAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGc                                                                                                                                                                                                                          <  2:134234/275‑1 (MQ=255)                                                                        gATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGc                                                                                                                                                                                                                          >  1:134234/1‑275 (MQ=255)                                                                           gtgtAGACAAGTGCATAGATGTCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAACTCCAGCAGGCTGACCGTGACGATGATACGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACTGCGAATCGCTTACAACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCAGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCAACTGTGACAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGc                                                                                                                                                                                              >  2:341379/1‑300 (MQ=255)                                                                           gtgtAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGTGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGc                                                                                                                                                                                              >  2:710638/1‑300 (MQ=255)                                                                                            gATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTACAACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCAAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCAACTGTGGCAGCACGCCGTAGAGGATCTATTAGAGCTTGTTGGCACCGGTGGCGCGAATGc                                                                                                                                                                             >  2:155713/1‑300 (MQ=255)                                                                                            gATATCAATGCCTAGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGc                                                                                                                                                                             >  1:756286/1‑300 (MQ=255)                                                                                                   aTGCCTCGCTTATCAGNTAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGTATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTNGAGCTTGTTGGCACCGGTNGCGCGAATGCCTTCCAc                                                                                                                                                                      >  1:714983/1‑300 (MQ=255)                                                                                                   aTGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCAc                                                                                                                                                                      <  1:408384/300‑1 (MQ=255)                                                                                                        tCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCAccgggc                                                                                                                                                                 <  2:127262/300‑1 (MQ=255)                                                                                                        tCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTATTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCAca                                                                                                                                                                     >  2:90633/1‑295 (MQ=255)                                                                                                                            ttACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCAc                                                                                                                                              <  1:243452/299‑1 (MQ=255)                                                                                                                                   aCCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTc                                                                                                                                        >  1:788732/1‑298 (MQ=255)                                                                                                                                        tGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAagc                                                                                                                                 <  1:518889/300‑1 (MQ=255)                                                                                                                                                 aaaTCCAGCAGGCTGACCTTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGTATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGgag                                                                                                                        <  2:112429/300‑1 (MQ=255)                                                                                                                                                 aaaTCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCATTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGgag                                                                                                                        <  2:30839/300‑1 (MQ=255)                                                                                                                                                     ccAGCAGGCTGACCGTNACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCAc                                                                                                                                                                      >  1:711992/1‑250 (MQ=255)                                                                                                                                                     ccAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCAc                                                                                                                                                                      <  2:711992/250‑1 (MQ=255)                                                                                                                                                     ccAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCAc                                                                                                                                                                      >  1:298084/1‑250 (MQ=255)                                                                                                                                                     ccAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCAc                                                                                                                                                                      <  2:298084/250‑1 (MQ=255)                                                                                                                                                       agcagGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGTTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCAATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCAc                                                                                                                   <  1:726720/299‑1 (MQ=255)                                                                                                                                                                      aCGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATa                                                                                                   >  1:194885/1‑300 (MQ=255)                                                                                                                                                                         atgatAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAAc                                                                                                <  1:150967/300‑1 (MQ=255)                                                                                                                                                                           gatAAGCACCAGCAGGGAGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAGGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCTTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCTGGCCGTGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCggtgg                                                                                                           <  2:71145/287‑1 (MQ=255)                                                                                                                                                                                               aGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGANGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCagcacgccagcacgcc                                                                          <  1:610572/300‑1 (MQ=255)                                                                                                                                                                                                ggTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCTTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAAc                                                                                                <  2:741352/277‑1 (MQ=255)                                                                                                                                                                                                ggTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAAc                                                                                                >  1:741352/1‑277 (MQ=255)                                                                                                                                                                                                                            gCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCa                                             >  1:347579/1‑300 (MQ=255)                                                                                                                                                                                                                                 ccACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGATGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCCGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTAGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAGCCCGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAAc                                        >  2:324926/1‑300 (MQ=255)                                                                                                                                                                                                                                 ccACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAAc                                        >  1:691295/1‑300 (MQ=255)                                                                                                                                                                                                                                 ccACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCAGTAGAGGATCTCTTCGAGTTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAGCACGACAGCACGCCAGCGAACGGGCAGAGGCCGCCGGCGACCACGAAc                                        >  2:207972/1‑300 (MQ=255)                                                                                                                                                                                                                                 ccACAGGGTGACGCCGATCCCGACTGCGCCGACCATACCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCCTCAACTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCCCCGGGCCGGGCTCCATCGCTTCCACCGCTCAGGAACGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAATAGCGCAAGCACGCCAGCACGCAAGCGAACGGGCCAAAGACGACGGCGACCCCGaa                                         >  2:324727/1‑299 (MQ=255)                                                                                                                                                                                                                                     aggttGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGATTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAACACGCCAGTGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAAc                                        <  2:673299/292‑1 (MQ=255)                                                                                                                                                                                                                                      gggTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAACAGCAt                                   >  1:828667/1‑300 (MQ=255)                                                                                                                                                                                                                                                gATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGATGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGCGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCACTCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCAAACGGGCCGAGGCCGACGGCGACCACGCACAGCATGGCGAAGAcc                         >  2:486058/1‑300 (MQ=255)                                                                                                                                                                                                                                                     cGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAACAGCATGGCGAAGACCATTTc                    >  2:744825/1‑300 (MQ=255)                                                                                                                                                                                                                                                                       cgacgaCGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAACAGCATGGCGAAGACCATTTCGTTAATGGCGCGGCAGGc  >  1:679702/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                  GCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTCGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAACAGCATGGCGAAGACCATTTCGTTAATGGCGCGGCAGGC  >  NC_000913/4322333‑4322894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑