Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,224,483	C→T	S219F (TCC→TTC)	metH →	cobalamin‑dependent methionine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,224,483	0	C	T	100.0%	193.4 / NA	58	S219F (TCC→TTC)	metH	cobalamin‑dependent methionine synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/30);  total (28/30)

CGCCAGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCGACCAACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTCCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAAC  >  NC_000913/4224192‑4224776                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                      cGCCAGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCGACCAACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCa                                                                                                                                                                                                                                                                                               >  2:783158/1‑300 (MQ=255)   ccAGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCGACCAACCGCACGGCGTCTATTTCTCAGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCAc                                                                                                                                                                                                                                                                                              >  1:2783/1‑299 (MQ=255)        gaAACCGCGCTACGTTGCCGGTGTTCTCGGCCCGACCAACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCtctc                                                                                                                                                                                                                                                                                        <  1:756056/300‑1 (MQ=255)        gaAACCGCGCTACGTTGCCGGTGTTCTCGGCCCGACCAACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACGGGCTGGTGGCGGCTTAGCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCGTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCGTCGGGCGCACGCgctc                                                                                                                                                                                                                                                                                        <  1:756045/300‑1 (MQ=255)                     gTTGCCGGTGTTCTCGGCCCGACCAACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGaccac                                                                                                                                                                                                                                                                            <  1:90789/299‑1 (MQ=255)                              gtTCTCGGCCCGACCAACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTTGGCGTTGAGCTGCCGATTATGATCTACGGCACCATCACCGACGACTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCAttt                                                                                                                                                                                                                                                                  >  1:577513/1‑300 (MQ=255)                              gtTCTCGGCCCGACCAACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCAttt                                                                                                                                                                                                                                                                  >  1:258269/1‑300 (MQ=255)                              gtTCTCGGCCCGACCAACCGCACGGCGTCTATTTCTACGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCAttt                                                                                                                                                                                                                                                                  >  1:258687/1‑300 (MQ=255)                               ttCTCGTCCCGACCAACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCAACGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCAtttt                                                                                                                                                                                                                                                                 >  1:36717/1‑300 (MQ=255)                                       ccGACCAACCGCACGGCGTCTATTTCTCAGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTACGGCACCATCAACGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCAttttcca                                                                                                                                                                                                                                                              >  2:188202/1‑292 (MQ=255)                                         gACCAACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTc                                                                                                                                                                                                                                                          <  1:783158/297‑1 (MQ=255)                                            cAACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTAACACTCAtt                                                                                                                                                                                                                                                       >  2:445633/1‑297 (MQ=255)                                             aaCCGCACGGCGTCTACTCCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTTGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTTAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTgcgc                                                                                                                                                                                                                                                   <  2:246698/300‑1 (MQ=255)                                                        gTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGc                                                                                                                                                                                                                                        >  1:727787/1‑300 (MQ=255)                                                         gcTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACGGGCTGGTGGCTGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGGATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGc                                                                                                                                                                                                                                        <  1:476240/298‑1 (MQ=255)                                                          cTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATTTGGTCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGCTTATGATCTACGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCAACGAAGCATTTTACAACTCATTGCGACAAGCCGAAgat                                                                                                                                                                                                                                       >  2:497790/1‑297 (MQ=255)                                                                           aatgATCCGGCATTTCGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAAc                                                                                                                                                                                                                     <  1:678997/297‑1 (MQ=255)                                                                           aaCGGTCCGGCATTTCTTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGGGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCTACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAAc                                                                                                                                                                                                                     <  2:166011/300‑1 (MQ=255)                                                                           aaCGATCCGGCATTTCGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAAc                                                                                                                                                                                                                     <  1:153553/300‑1 (MQ=255)                                                                           aaCGATCCGGCATTTCGTAATATCACTTTTAACGTGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAAc                                                                                                                                                                                                                     <  2:533840/300‑1 (MQ=255)                                                                            aCGATCCGGCATTTCGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGANGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAAc                                                                                                                                                                                                                     <  2:604026/299‑1 (MQ=255)                                                                              gATCCGGCATTTCGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACtg                                                                                                                                                                                                                   <  1:693649/299‑1 (MQ=255)                                                                               aTCCGGCATTTCGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGaa                                                                                                                                                                                                                      >  1:691058/1‑295 (MQ=255)                                                                                                 tCACTTTTGACGGGCTGTTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAAc                                                                                                                                                                                               <  1:527114/300‑1 (MQ=255)                                                                                                 tCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAAc                                                                                                                                                                                               <  1:527124/300‑1 (MQ=255)                                                                                                                 ggtggCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCa                                                                                                                                                                               >  1:414798/1‑300 (MQ=255)                                                                                                                     gcggcTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAg                                                                                                                                                                           <  2:575562/300‑1 (MQ=255)                                                                                                                     gcggcTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAg                                                                                                                                                                           <  1:547305/300‑1 (MQ=255)                                                                                                                        gcTTATCGAGAGTCCACTAAAACGCTGGTGGAAGTTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCTGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGc                                                                                                                                                                                <  2:225002/292‑1 (MQ=255)                                                                                                                         cTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTACGGCACCATCAACGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCGTTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCATGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAg                                                                                                                                                                           >  2:181020/1‑296 (MQ=255)                                                                                                                         cTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCAGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAg                                                                                                                                                                           >  2:566778/1‑296 (MQ=255)                                                                                                                                agagTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGAt                                                                                                                                                                <  1:274025/300‑1 (MQ=255)                                                                                                                                  agTCCACCAAAGCGCTGGTGGAAGGTTGCATGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTGAACGCCAAAGCGGCGGTATGTGCGGTGAAAACTGAGTTTGAAGNTCTGGTCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATGTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCTATGAACTGCGCCAGTAc                                                                                                                                                                                    <  2:264742/278‑1 (MQ=255)                                                                                                                                   gTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGc                                                                                                                                                             >  1:350974/1‑300 (MQ=255)                                                                                                                                        ccaAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAAt                                                                                                                                                        >  1:516119/1‑300 (MQ=255)                                                                                                                                          aaaGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCGTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGTGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGc                                                                                                                                                      <  1:400547/300‑1 (MQ=255)                                                                                                                                                  ggtggAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCAc                                                                                                                                              >  1:677376/1‑300 (MQ=255)                                                                                                                                                   gtggAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTACGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCAGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACATTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGCATGCTACGTCAcc                                                                                                                                             >  2:334012/1‑300 (MQ=255)                                                                                                                                                   gtggAAGGTGGCGCGGATCTGATCCTGATCGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGTGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTACGGCACCATCAACGACGCCTTAGGGCGCACGCTCTCCGGGCAGACCAACGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTAGGGCACGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCAcc                                                                                                                                             >  2:240845/1‑300 (MQ=255)                                                                                                                                                     ggAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGAAGCTGTCACGGATTGCGGCATGCTACGTCACcgc                                                                                                                                           >  2:50514/1‑300 (MQ=255)                                                                                                                                                             gcgcGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTAAGTCACCGCGCACCCGa                                                                                                                                   >  2:823350/1‑300 (MQ=255)                                                                                                                                                                                ttaaaCCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTTAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCACGCACCCGAACGCCGGGCTACCCAACGc                                                                                                                <  2:99196/298‑1 (MQ=255)                                                                                                                                                                                tGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGANCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGc                                                                                                                <  1:620959/300‑1 (MQ=255)                                                                                                                                                                                tGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCTGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGc                                                                                                                <  1:128881/300‑1 (MQ=255)                                                                                                                                                                                        ttttCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACNACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTg                                                                                                        <  2:258687/300‑1 (MQ=255)                                                                                                                                                                                        ttttCGACACCATGAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTTAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCTGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACNACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTg                                                                                                        <  2:258269/300‑1 (MQ=255)                                                                                                                                                                                                  ccTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGAtct                                                                                              >  2:801865/1‑300 (MQ=255)                                                                                                                                                                                                      aaCGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGAc                                                                                          >  1:638702/1‑300 (MQ=255)                                                                                                                                                                                                              atcggcggTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTAGGATCTCCGGCACCATCACCGACGCCTTCGTGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCTCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGacac                                                                                  <  2:2783/298‑1 (MQ=255)                                                                                                                                                                                                                                 aacgcttattttGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAAc                                                                                                                                                                                                                                                            <  1:842430/102‑1 (MQ=255)                                                                                                                                                                                                                                                gcgcTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGc                                                 >  1:507542/1‑299 (MQ=255)                                                                                                                                                                                                                                                gcgcTGGGCGTTGAGCTGCCGATTATGATCTACGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGc                                                 >  1:651847/1‑299 (MQ=255)                                                                                                                                                                                                                                                     gggCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCNACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGttt                                           >  1:600880/1‑300 (MQ=255)                                                                                                                                                                                                                                                                     aTTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTcggc                           >  2:792322/1‑300 (MQ=255)                                                                                                                                                                                                                                                                             ctcCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGNTCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGgctgct                    >  1:159054/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                    aCCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGANGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGcac             <  1:597256/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                    aCCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGcac             <  1:823350/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                                gCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCacaac  <  2:730997/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                      CGCCAGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCGACCAACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTCCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAAC  >  NC_000913/4224192‑4224776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑