Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,160,157	G→A	L212L (CTG→TTG)	sthA ←	soluble pyridine nucleotide transhydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,160,157	0	G	A	100.0%	318.3 / NA	96	L212L (CTG→TTG)	sthA	soluble pyridine nucleotide transhydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (46/50);  total (46/50)

ACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAGTGTTTCAACGCTGCCGTCCGGGCAATCCAGCGCCAACGTATGCTCGTCAACAAAGCGAGCG  >  NC_000913/4159865‑4160443                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                               aCCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAgg                                                                                                                                                                                                                                                                                            <  1:625363/297‑1 (MQ=255)    gcgTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGNCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATc                                                                                                                                                                                                                                                                                       >  2:610971/1‑299 (MQ=255)          aCGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGgt                                                                                                                                                                                                                                                                                 >  1:740235/1‑299 (MQ=255)          aCGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTAGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTATTGATCGAGAAATGCCAACAGGCGATCGCGGgt                                                                                                                                                                                                                                                                                 >  1:476212/1‑299 (MQ=255)                ggCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATc                                                                                                                                                                                                                                                                          <  1:296729/300‑1 (MQ=255)                 gCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑CGGAGAGAGAATCTGACATCTCTTGATCGAGGAATGCCAACAGGCGATCGCGGGTGTTGATCa                                                                                                                                                                                                                                                                         >  2:62496/1‑300 (MQ=255)                         gTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCGGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACTGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCAGGTGTTGATCAGATcccat                                                                                                                                                                                                                                                                 >  2:369551/1‑297 (MQ=255)                         gTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCANAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGCTCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACt                                                                                                                                                                                                                                                                 >  1:179437/1‑300 (MQ=255)                         gTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACt                                                                                                                                                                                                                                                                 >  2:94053/1‑300 (MQ=255)                         gTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACt                                                                                                                                                                                                                                                                 >  1:55950/1‑300 (MQ=255)                         gTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTATTAATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATcc                                                                                                                                                                                                                                                                    >  2:369789/1‑297 (MQ=255)                           cTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACttt                                                                                                                                                                                                                                                               >  2:810707/1‑300 (MQ=255)                             taTACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACtttt                                                                                                                                                                                                                                                              >  1:439016/2‑299 (MQ=255)                             gATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTa                                                                                                                                                                                                                                                             >  1:106095/1‑300 (MQ=255)                                     cTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTATTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATa                                                                                                                                                                                                                                                     >  1:454055/1‑300 (MQ=255)                                      tGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATAc                                                                                                                                                                                                                                                    <  1:722664/300‑1 (MQ=255)                                      tGTTGACCTGCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATAc                                                                                                                                                                                                                                                    <  1:722651/300‑1 (MQ=255)                                           aCCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGGAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAATAGGCGATCGCGGGTGTTGATCCGCTCCACTTTTACATcccta                                                                                                                                                                                                                                                     >  2:236630/1‑291 (MQ=255)                                           aCCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACcgc                                                                                                                                                                                                                                                 >  2:787676/1‑298 (MQ=255)                                             cTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCTACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCAAGAAGTGAT‑AGGAGAGAGAATCTGACATCTATTGATCGAGAAATGCAAACAGGCGATCGCGGGTGTTGATCAGACCCACTTTTACATCCATACCGCGGaa                                                                                                                                                                                                                                             >  1:277285/1‑300 (MQ=255)                                                cAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGNCATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGAt                                                                                                                                                                                                                                          >  1:617259/1‑300 (MQ=255)                                                     gTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTATTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGAc                                                                                                                                                                                                                                      >  1:590124/1‑299 (MQ=255)                                                              tGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTcac                                                                                                                                                                                                                            <  2:816488/300‑1 (MQ=255)                                                                  agtgtCTAGCCCAATGTTCTGTAACGCCGGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCTAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGc                                                                                                                                                                                                                         <  2:207956/295‑1 (MQ=255)                                                                        tAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCAc                                                                                                                                                                                                                  <  1:224415/300‑1 (MQ=255)                                                                          gCCCCATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTCCCCGACTTCAGATGCATGATCACACCATCGTCAAAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGGGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTc                                                                                                                                                                                                                >  1:312795/1‑300 (MQ=255)                                                                                tGTTCTGTAACGCCAGCGAATCGTTATTACCGTTGCTACCGTTTGCATAGAGCAGGCATTCAGCTTTCAGTTTTTTACCCGACTTCAGAGGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCNAGAAGTGAT‑AGGATAGAGAATCTGACATCTCTNGATCGATAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTGCATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCAc                                                                                                                                                                                                          <  2:634707/300‑1 (MQ=255)                                                                                tGTTCTGTAACGCCAGCGAATCGGTATTACCGGTTCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCAc                                                                                                                                                                                                          <  2:522264/300‑1 (MQ=255)                                                                                tGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCNAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTNGATCTAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCAc                                                                                                                                                                                                          <  2:634457/300‑1 (MQ=255)                                                                                tGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCAc                                                                                                                                                                                                          <  1:82682/300‑1 (MQ=255)                                                                                tGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGACCACTCCAGCAc                                                                                                                                                                                                          <  1:48029/300‑1 (MQ=255)                                                                                tGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAAAAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCAc                                                                                                                                                                                                          <  1:532202/300‑1 (MQ=255)                                                                                 gTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTAGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTATTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTCCATCCATACCGCGGAAGATCGAAGCATATTCACAGCCGATCACTCCAGCAcc                                                                                                                                                                                                         >  1:528408/1‑300 (MQ=255)                                                                                   tCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGCCATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCAt                                                                                                                                                                                                       >  2:59058/1‑300 (MQ=255)                                                                                        aaCGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTTTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATa                                                                                                                                                                                                  <  2:144562/300‑1 (MQ=255)                                                                                        aaCGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCTAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATa                                                                                                                                                                                                  <  2:152057/300‑1 (MQ=255)                                                                                           gCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAg                                                                                                                                                                                                <  1:767446/299‑1 (MQ=255)                                                                                              aGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGTAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTAc                                                                                                                                                                                             <  2:778593/299‑1 (MQ=255)                                                                                              aGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGNGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTAc                                                                                                                                                                                             <  2:617259/299‑1 (MQ=255)                                                                                              aGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGGTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTAc                                                                                                                                                                                             <  1:830415/299‑1 (MQ=255)                                                                                              aGCGAATCAGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTAc                                                                                                                                                                                             <  2:163072/299‑1 (MQ=255)                                                                                                  aaTCGGTATTACCGGTGCGACCGTTGGCATAGAGCATGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTAc                                                                                                                                                                                             <  2:163062/295‑1 (MQ=255)                                                                                                       gTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGAAGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTAAATGGCGCGGt                                                                                                                                                                                   >  2:433146/1‑300 (MQ=255)                                                                                                       gTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTATTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGt                                                                                                                                                                                   >  2:564203/1‑300 (MQ=255)                                                                                                         aTTACCGGTTCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTc                                                                                                                                                                                 <  2:535548/300‑1 (MQ=255)                                                                                                               gttGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCgtgg                                                                                                                                                                             <  2:520967/296‑1 (MQ=255)                                                                                                               ggttcGACCGTTGGCGTAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGTAGAGAGAATCTGACATCCCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCgtggt                                                                                                                                                                            <  2:331008/295‑1 (MQ=255)                                                                                                               ggTGCGACCGTTGGCANAGAGCAGGCAGTCAGCTTNCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACNTGGCGCGGTTCgtggt                                                                                                                                                                            <  1:717017/299‑1 (MQ=255)                                                                                                                  gCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCAt                                                                                                                                                                        <  1:407243/300‑1 (MQ=255)                                                                                                                       cGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAGGTACATGGCGCGGTTCGTGGTGCATGCTga                                                                                                                                                                   >  2:704285/1‑300 (MQ=255)                                                                                                                           ggCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGTAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAAt                                                                                                                                                               <  1:462509/300‑1 (MQ=255)                                                                                                                           ggCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAAt                                                                                                                                                               <  1:462309/300‑1 (MQ=255)                                                                                                                           ggCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTGTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAAt                                                                                                                                                               <  1:462540/300‑1 (MQ=255)                                                                                                                            gCATAGAGCAGGCAGTCAGTTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTAGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAAtt                                                                                                                                                              >  2:283318/1‑300 (MQ=255)                                                                                                                            gCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAAtt                                                                                                                                                              >  1:568078/1‑300 (MQ=255)                                                                                                                                agagCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTCCTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTATTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACTATAGATAAGCACATGGCGAGGTTCGTGGTGCATGCTGAGAATTGAGt                                                                                                                                                          >  2:390263/1‑300 (MQ=255)                                                                                                                                agagCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTAGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTATTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGAAGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGAGGTTCGTGGTGCATGCTGAGAATTGAGt                                                                                                                                                          >  2:390364/1‑300 (MQ=255)                                                                                                                                                                             tGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGATAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCGCTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGgat                                                                                                             <  2:740235/300‑1 (MQ=255)                                                                                                                                                                              gATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGgat                                                                                                             >  1:568884/1‑299 (MQ=255)                                                                                                                                                                                tCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGNGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGgatgat                                                                                                          >  1:188554/1‑300 (MQ=255)                                                                                                                                                                                tCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTATTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGgatgat                                                                                                          >  2:555428/1‑300 (MQ=255)                                                                                                                                                                                   cacCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTATTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGatat                                                                                                        >  1:451891/1‑299 (MQ=255)                                                                                                                                                                                       aTCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACg                                                                                                   <  2:484545/300‑1 (MQ=255)                                                                                                                                                                                       aTCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAAATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACg                                                                                                   <  2:172012/300‑1 (MQ=255)                                                                                                                                                                                          gtcccaGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACgaga                                                                                                >  1:543577/5‑300 (MQ=255)                                                                                                                                                                                          gTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCAAGAAGTGAT‑AGGAGAGAGAATCTGACATCTATTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACgaga                                                                                                >  1:494252/1‑300 (MQ=255)                                                                                                                                                                                             acatccTTCGATCTTCTCTTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCATAAGTGAT‑AGGATAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCAATCACTCCAGCACCATAGATAAGTACATGGCGCGGTGCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGc                                                                                              <  2:439016/295‑1 (MQ=255)                                                                                                                                                                                                gCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCa                                                                                          >  1:66371/1‑300 (MQ=255)                                                                                                                                                                                                gCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTNACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCa                                                                                          >  2:604866/1‑300 (MQ=255)                                                                                                                                                                                                          ttctCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCATAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGc                                                                                                                                                                                                                         <  2:468554/163‑1 (MQ=255)                                                                                                                                                                                                          ttctCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGc                                                                                                                                                                                                                         >  1:468554/1‑163 (MQ=255)                                                                                                                                                                                                           tctcGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTATTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACaa                                                                               >  1:323082/1‑300 (MQ=255)                                                                                                                                                                                                                                   tCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAGTGTTTCaa                                                       <  1:787676/300‑1 (MQ=255)                                                                                                                                                                                                                                     aCTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAGTGTTTCAAc                                                      <  1:94053/299‑1 (MQ=255)                                                                                                                                                                                                                                     aCTACGCCACTGGTCCAGAATTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAGTGTTTCAACg                                                     <  2:409362/300‑1 (MQ=255)                                                                                                                                                                                                                                      cTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTATTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAGTGTTTCAACGc                                                    >  1:632332/1‑300 (MQ=255)                                                                                                                                                                                                                                       tACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCTAGAAATGCCAACATGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAGTGTTTCAACGc                                                    <  1:281966/299‑1 (MQ=255)                                                                                                                                                                                                                                       tACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAGTGTTTCAACGc                                                    <  1:282082/299‑1 (MQ=255)                                                                                                                                                                                                                                           ccACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAGTGTTTCAACGCTGcc                                                >  2:682196/1‑299 (MQ=255)                                                                                                                                                                                                                                               tgtcccAGAAGTGATCNGGAGAGAGAATCTGACATNTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAGTNTTTCAACGCTGCCGTc                                             <  1:704285/295‑1 (MQ=255)                                                                                                                                                                                                                                               tGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAGTGTTTCAACGCTGCCGTcc                                            <  1:334136/299‑1 (MQ=255)                                                                                                                                                                                                                                                  tCCATAAGTGAT‑AGGAGAGAAAATCTGACATCTCTTGAGCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTCTACATCCAGACCGCGGAAGATCGACGCATAGTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACTAGAGCCGCAGGCAATAACAAATTTTTCAGCGGGTAGTGTTTCAACGCTGCCGTCCGGGc                                        <  2:328989/300‑1 (MQ=255)                                                                                                                                                                                                                                                       aaGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAGTGTTTCAACGCTGCCGTCCGGGCAATcc                                   <  1:558324/300‑1 (MQ=255)                                                                                                                                                                                                                                                        aGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAAAAAATTTTTCAGCGGTTAGTGTTTCAACGCTGCCGTCCGGGCAACCCa                                  >  2:32950/1‑300 (MQ=255)                                                                                                                                                                                                                                                          tGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAGTGTTTCAACGCTGCCGTCCGGGCAATcc                                   >  2:34696/1‑297 (MQ=255)                                                                                                                                                                                                                                                          tGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCNGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAGTGTTTCAACGCTGCCGTCCGGGCAATCCAgc                                >  2:267809/1‑300 (MQ=255)                                                                                                                                                                                                                                                            aT‑AGGAGAGAGAATCTGACATCTATTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAGTGTTTCAACGCTGCCGTCCGGGCAATCCAgcgc                              >  2:719655/1‑300 (MQ=255)                                                                                                                                                                                                                                                                      agaATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAGTGTTTCAACGCTGCCGTCCGGGCAATCCAGCGCCAACGTATg                     <  1:484687/300‑1 (MQ=255)                                                                                                                                                                                                                                                                       gaATCTGACATCTCTTGATCTAGAAATGCCAACAGGCAATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATTGCGCGGTGCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAGTGTTTCAACGCTGCCGTCCGGGCAATCCAGCGCCGACGTATGc                    <  2:106095/300‑1 (MQ=255)                                                                                                                                                                                                                                                                       gaATCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAGTGTTTCAACGCTGCCGTCCGGGCAATCCAGCGCCAACGTATGc                    <  1:109811/300‑1 (MQ=255)                                                                                                                                                                                                                                                                        aaTCTGACATCTCTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAGTGTTTCAACGCTGCCGTCCGGGCAATCCAGCGCCAACGTATGCt                   <  1:630945/300‑1 (MQ=255)                                                                                                                                                                                                                                                                         aTCTGACATCTCTTGATCTAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAGTGTTTCAACGCTGCCGTCCGGGCAATCCAGCGCCAACGTATGCTc                  <  1:640341/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                 tctcTTGATCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAGTGTTTCAACGCTGCCGTCCGGGCAATCCAGCGCCAACGTATGCTCGTcaacaa          >  2:431720/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                 tctcTTGATCGAGAAATGCAAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCAAAGCAGATCACTCCAGCACAATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAGTGTTTCAACGCTGCCGTCCGGGCAATCCAGCGCCAACGTATGCTCGTcaacaa          >  2:431035/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                         tCGAGAAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAGTGTTTCAACGCTGCCGTCCGGGCAATCCAGCGCCAACGTATGCTCGTCAACAAagcgagcg  <  1:758292/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                           gagaAATGCCAACAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATTGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAGTGTTTCAACGCTGCCGTCCGGGCAATCTAGCGCCAACGTATGCTCGTCAACAAagcgagcg  <  1:758281/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                               ACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGAT‑AGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAGTGTTTCAACGCTGCCGTCCGGGCAATCCAGCGCCAACGTATGCTCGTCAACAAAGCGAGCG  >  NC_000913/4159865‑4160443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑