Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,928,064	C→T	L304L (CTG→TTG)	asnA →	asparagine synthetase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,928,064	0	C	T	98.3%	201.9 / NA	59	L304L (CTG→TTG)	asnA	asparagine synthetase A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (31/27);  minor base G (1/0);  total (32/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGA‑GCTGGGCCATGCGGGTCT‑GAACGG‑‑CGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCC‑GACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCCGCTGCAGCTCTTTCACTTTACTCGTCACGTCGTCAGGCAACCGCTGAGCGATAAAATCAGAAATCACCACC  >  NC_000913/3927773‑3928356                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                       gATGGTCATCGCCACGACATGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGA‑GCTGGGCCATGCGGTTCT‑GAACGG‑‑CGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCC‑GACACGCTTAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCa                                                                                                                                                                                                                                                                                                 <  1:295753/299‑1 (MQ=255)         tCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGA‑GCTGGGCCATGCGGGTCT‑GAACGG‑‑CGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCC‑GACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCa                                                                                                                                                                                                                                                                                        >  2:429044/1‑300 (MQ=255)           gCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGA‑GCTGGGCCATGCGGGTCT‑GAACGG‑‑CGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCC‑GACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCata                                                                                                                                                                                                                                                                                      >  1:379291/1‑300 (MQ=255)                   gTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGA‑GCTGGGCCATGCGGGTCT‑GAACGG‑‑CGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTTTAGATGCC‑GACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCa                                                                                                                                                                                                                                                                               >  1:697531/1‑299 (MQ=255)                       tcgcACCTGATTATGATGACTGGAGCACCCCGGCAGA‑GCTGGTACATGCGGGTCT‑GAACGG‑‑CGATATTCTGATGGGGAACCCGATACTGGAAGATGCGGTTGAGCCTGCTTCCAGGGGGATCCGTGTAGATGCC‑GACATGCTTAAGCATCAACGGGCGCTGACCGGTGACGAAGATCGCCGGGAGCTGGAGTGGCAGCAGGCGCTGCTGCGCGGTGAAATGCCGTAGATCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGTCGCATATTGGCCAAG‑‑TTc                                                                                                                                                                                                                                                                        <  2:719653/299‑1 (MQ=255)                       gcgcACCGGATTATGATGACTGGAGCACCCCGTCAGA‑GCTGGGCCATGCGGGTCT‑GAACGG‑‑CGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCC‑GACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTc                                                                                                                                                                                                                                                                        <  2:719682/300‑1 (MQ=255)                              ggATTATGATGACTGGAGCACCCCGTCAGA‑GCTGGGCCATGCGGGTCT‑GAACGG‑‑CGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGTC‑GACACGCTGAAGCATCAACTGGCGCTGACCGGTGAAGAGGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTAAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTATCACTTGACTATGCTGGTGCTGCAACTGCCGCATATCGGACAGG‑‑TTCAgtgt                                                                                                                                                                                                                                                                   >  2:410884/1‑298 (MQ=255)                              ggATTATGATGACTGGAGCACCCCGTCAGA‑GCTGGGCCATGCGGGTCT‑GAACGG‑‑CGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCC‑GACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAAATGCCGCATATCGGCCAGG‑‑TTCAgtgt                                                                                                                                                                                                                                                                   >  2:50309/1‑298 (MQ=255)                                 ttATGATGACTGGAGCACCCCGTCAGA‑GCTGGGCCATGCGGGTCT‑GAACGG‑‑CGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCC‑GACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGt                                                                                                                                                                                                                                                              <  1:627201/300‑1 (MQ=255)                                      atgacgtgAGCACCCCGTCAGA‑GCTGGGCCAGGCGGGTCT‑GAACGG‑‑CGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGTGGATCCGTGTAGATGCC‑GACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGc                                                                                                                                                                                                                                                         <  2:285487/293‑1 (MQ=255)                                      atgaCTGGAACACCCCGTCAGA‑GCTGGGCCATGCGGGTCT‑GAACGG‑‑CGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCC‑GACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGc                                                                                                                                                                                                                                                         <  2:703593/300‑1 (MQ=255)                                          cTGGAGCACCCCGTCAGA‑GCTGGGCCATGCGGGTCT‑GAACGG‑‑CGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCC‑GACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAgc                                                                                                                                                                                                                                                     >  1:287291/1‑300 (MQ=255)                                           ggagccACCCCGTCAGNCACTGGGCCATGCGGGTCNCGAACGGGCCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGGNCTTCAGTGTGGAGTAt                                                                                                                                                                                                                                                            <  1:604202/294‑1 (MQ=255)                                                  ccccGTCAGA‑GCTGGGCCATGCGGGTCT‑GAACGG‑‑CGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCC‑GACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCAGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTc                                                                                                                                                                                                                                             >  1:258378/1‑300 (MQ=255)                                                     cGTCAGA‑GCTGGGCCATGCGGGTCT‑GAACGG‑‑CGATATTCTGGTGTGGAACACGGTACTGGAAGATGTGTTTGAGCTTTCATCCATGGGGATCCGTGTAGATGCC‑GACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTAGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTcgc                                                                                                                                                                                                                                           >  2:85814/1‑299 (MQ=255)                                                       tCAGA‑GCTGTGCCAGGCGGGTCT‑GAACGG‑‑CCATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCC‑GACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCGGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCgag                                                                                                                                                                                                                                        <  2:609842/300‑1 (MQ=255)                                                          ga‑gCTGGGCCATGCGGGTCT‑GAACGG‑‑CGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCC‑GACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCATGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCAGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGc                                                                                                                                                                                                                                     >  2:159691/1‑300 (MQ=255)                                                                 ggCCATGCGGGTCT‑GAACGG‑‑CGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCGTGGGGATCCGTGTAGATGCC‑GACACGCTGNAGCATCAACTGGCGCTGACCGGTGACGAAGATNGACTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCt                                                                                                                                                                                                                               >  2:549989/1‑300 (MQ=255)                                                                 ggCCATGCGGGTCT‑GAACGG‑‑CGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCC‑GACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCt                                                                                                                                                                                                                               >  1:79036/1‑300 (MQ=255)                                                                 ggCCATGCGGGTCT‑GAACGG‑‑CGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCC‑GACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑CTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCt                                                                                                                                                                                                                               >  1:78683/1‑300 (MQ=255)                                                                     aTGCGGGTCT‑GAACGT‑‑CTATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCC‑GACACGCTTAAGCATCAACTGGCGCTGACCGTTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTtctc                                                                                                                                                                                                                           <  2:396989/300‑1 (MQ=255)                                                                      tGCGGGTCT‑GAACGG‑‑CGATATTTTGGGGTGGAACCCGGTACTGTAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCC‑GACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGATTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCTGCCAGTTTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTtccc                                                                                                                                                                                                                           <  2:396983/299‑3 (MQ=255)                                                                         gggTCT‑GAACGG‑‑CGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCC‑GACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTctgc                                                                                                                                                                                                                        <  1:298466/299‑1 (MQ=255)                                                                                                         aCCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCC‑GACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAg                                                                                                                                                                                          <  1:403871/300‑1 (MQ=255)                                                                                                             ggTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCC‑GACACGCTGAAGCATCAACTGGCNCTTACCGTTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTAGCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCGGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGc                                                                                                                                                                                         <  1:525622/297‑1 (MQ=255)                                                                                                              gTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCC‑GACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTATGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAggc                                                                                                                                                                                     >  1:723182/1‑300 (MQ=255)                                                                                                               tACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTTTAGATGCC‑GACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAggcg                                                                                                                                                                                    <  2:697531/300‑1 (MQ=255)                                                                                                                                          ccATGGGGATCCGTGTAGATGCC‑GACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCa                                                                                                                                                         >  2:129758/1‑300 (MQ=255)                                                                                                                                          ccATGGGGATCCGTGTAGATGCC‑GACACGCTGAAGCATCAACTGGCGCTGACCGATGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTCCGCGAGAGCGCCCCTTCTATGCTGTAATAATTTATCGCCAACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTACCAAAGCGCCa                                                                                                                                                         >  2:3667/1‑300 (MQ=255)                                                                                                                                              gtggATCCGTGTAGATGCC‑GACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGGGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGata                                                                                                                                                     <  1:126710/298‑1 (MQ=255)                                                                                                                                              ggggATCCGTGTAGATGCC‑GACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGata                                                                                                                                                     <  1:643400/300‑1 (MQ=255)                                                                                                                                                   tCCGTGTAGATGCC‑GACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGTGGCTTCGCATCCAGGTATCAAAGCGCCAGATATGATc                                                                                                                                                >  2:289960/1‑300 (MQ=255)                                                                                                                                                   tCCGTGTAGATGCC‑GACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCATTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCAGGTATCAAAGCGCCAGATATGATc                                                                                                                                                >  2:379749/1‑300 (MQ=255)                                                                                                                                                      gtgtAGATGCC‑GACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGaa                                                                                                                                             >  1:581378/1‑300 (MQ=255)                                                                                                                                                      gtgtAGATGCC‑GACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCATTCTATGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGaa                                                                                                                                             >  2:433398/1‑300 (MQ=255)                                                                                                                                                                                tCAACTGGCGCTGACCGTTGACGAGGATCGCCTGGAGCTGGAGTGGTATCATGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGGGGGGTATNGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCNGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCg                                                                                                                    <  2:544381/300‑1 (MQ=255)                                                                                                                                                                                tCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATNGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCNGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCg                                                                                                                    <  2:544410/300‑1 (MQ=255)                                                                                                                                                                                  aaCTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCNTATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATACGATCGAAAATGCGCACGATGCAGGGTTTGCCgt                                                                                                                   >  2:538460/1‑299 (MQ=255)                                                                                                                                                                                  aaCTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCNTATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTATGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCgt                                                                                                                   >  2:527335/1‑299 (MQ=255)                                                                                                                                                                                                                  gagctggagTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCt                                                                                  >  1:248314/1‑300 (MQ=255)                                                                                                                                                                                                                          gttgCATCAGGCGCTGCTGCGCGGGGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTAc                                                                           <  2:351484/296‑1 (MQ=255)                                                                                                                                                                                                                           tGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTAccc                                                                         <  1:22716/300‑1 (MQ=255)                                                                                                                                                                                                                           tGGCATCAGGCGCTACTGCGCGGTGAAACGCCGCAGACCATCGGCGGCGGTAGCGGCCAATCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTAccc                                                                         <  2:217465/300‑1 (MQ=255)                                                                                                                                                                                                                            ggCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCNGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTCNCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCAGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTAccc                                                                         >  2:550860/1‑299 (MQ=255)                                                                                                                                                                                                                            ggCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGtaata                                                                                                                                                                                                                 <  1:1877/163‑1 (MQ=255)                                                                                                                                                                                                                            ggCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGtaata                                                                                                                                                                                                                 >  2:1877/1‑163 (MQ=255)                                                                                                                                                                                                                                   ggcactgctgCGCGGGGAAATGCCGCAGACCATCGGCGTCGGTATCGTCCAGTCTCGTTTTACTAGGCTGTTGCTGCAACTTCCGCATATCGTCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTTTAATAATTTAGCGCCACCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCTGCTGCAGc                                                                 <  2:721900/296‑1 (MQ=255)                                                                                                                                                                                                                                      gctgctGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCCGCTGCAGctc                                                               <  1:55549/299‑1 (MQ=255)                                                                                                                                                                                                                                      gctgctGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCCGCTGCAGctc                                                               <  2:731205/299‑1 (MQ=255)                                                                                                                                                                                                                                                                  aTCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCCGCTGCAGCTCTTTCACTTTACTCGTCACGTCGTCAGGCa                                  <  1:429044/300‑1 (MQ=255)                                                                                                                                                                                                                                                                       cggcggTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCATTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCAGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCCGCTGCAGCTCTTTCACTTTACTCGTCACGTCGTCAGGCAACCGc                             >  2:364186/1‑300 (MQ=255)                                                                                                                                                                                                                                                                            gTATCGGCCGGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCACATATCGGCTATG‑‑TTCAGTTTGGAGTATGGCCAGCTGCTGTTCGTGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCACCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAACGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCCGCTGCAGCTCTTTCACTTTACTCGTCACGTCGTCAGGCAACCGCTGAGc                        <  2:287291/300‑1 (MQ=255)                                                                                                                                                                                                                                                                            gTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCCGCTGCAGCTCTTTCACTTTACTCGTCACGTCGTCAGGCAACCGCTGAGc                        >  1:569727/1‑300 (MQ=255)                                                                                                                                                                                                                                                                            gTATCGGCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCCGCTGCAGCTCTTTCACTTTACTCGTCACGTCGTCAGGCAAACGCTGa                          >  2:351123/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                 ggCCAGTCTCGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCCGCTGCAGCTCTTTCACTTTACTCGTCACGTCGTCAGGCAACCGCTGAGCGATaa                   >  2:707272/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                          cGTTTGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCAGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCCGCTGCAGCTCTTTCACTTTACTCGTCACGTCGTCAGGCAACCGCTGAGCGATAAAATCAGaaa          >  2:299161/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                              tGACTATGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCCGCTGCAGCTCTTTCACTTTACTCGTCACGTCGTCAGGCAACCGCTGAGCGATAAAATCAGAAATcac      >  1:442876/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                   aTGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCCGCTGCAGCTCTTTCACTTTACTCGTCACGTCGTCAGGCAACCGCTGAGCGATAAAATCAGAAATcacca    >  2:344804/1‑297 (MQ=255)                                                                                                                                                                                                                                                                                                   aTGCTGTTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCCGCTGCAGCTCTTTCACTTTACTCGTCACGTCGTCAGGCAACCGCTGAGCGATAAAATCAGAAATCaccacc  >  1:216424/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                       GATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGA‑GCTGGGCCATGCGGGTCT‑GAACGG‑‑CGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCC‑GACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGG‑‑TTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCCGCTGCAGCTCTTTCACTTTACTCGTCACGTCGTCAGGCAACCGCTGAGCGATAAAATCAGAAATCACCACC  >  NC_000913/3927773‑3928356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑