Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,787,021	G→A	A62T (GCA→ACA)	envC →	murein hydrolase activator EnvC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,787,021	0	G	A	100.0%	187.4 / NA	58	A62T (GCA→ACA)	envC	murein hydrolase activator EnvC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/29);  total (29/29)

GCGCCGACCATGTTGTCGCTGATGGGTATGGAAATCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGC  >  NC_000913/3786727‑3787310                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                  gcgcCGACCATGTTGTCGCTGATGGGTATGGAAATCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGc                                                                                                                                                                                                                                                                                              >  1:505100/1‑300 (MQ=255)                        gggTATGGAAATCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCa                                                                                                                                                                                                                                                                       >  1:627885/1‑300 (MQ=255)                         ggTATGGAAATCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCATGCTCGCACAATTGAAAAAGCa                                                                                                                                                                                                                                                                       >  2:636457/1‑299 (MQ=255)                          gTATGGAAATCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCa                                                                                                                                                                                                                                                                       >  1:95288/1‑298 (MQ=255)                          gTATGGAAATCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCACGTGGGGGGAAAGGTGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCAAGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACCTCCACGAAAAAGAGCGCACGGCACACCAAAAACACCACAAAAACACAAGACTGTTCGCACAAGTGAAAAAgaa                                                                                                                                                                                                                                                                       >  2:413882/1‑296 (MQ=255)                                            agaTGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAg                                                                                                                                                                                                                                                   <  1:424261/300‑1 (MQ=255)                                             gaTGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGc                                                                                                                                                                                                                                                  <  2:444764/300‑1 (MQ=255)                                                  cTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCAccc                                                                                                                                                                                                                                             >  1:497775/1‑300 (MQ=255)                                                        aGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGc                                                                                                                                                                                                                                       <  1:505889/300‑1 (MQ=255)                                                        aGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGc                                                                                                                                                                                                                                       <  2:316419/300‑1 (MQ=255)                                                                   aTCGTGGAATAATCCCTCCCCATGATGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTACTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAAc                                                                                                                                                                                                                             <  2:717207/299‑1 (MQ=255)                                                                         gAATAATCCCTCCCAATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCATTTTACGCCCACGCGGATGAGCGTGAACAACTCAAATCTATTCAGGCTGACATCGCCGAAAAGGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAac                                                                                                                                                                                                                      >  2:198373/1‑300 (MQ=255)                                                                                  ctcccCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCNTATTGTTGTGCGCCTTTTCCGCCCACGCGGATNAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGAACAAGCCTGCTCGCCCAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTCAGCTGCGCGAAACGCAAAACACGCtcaat                                                                                                                                                                                                             >  2:555682/1‑300 (MQ=255)                                                                                  ctcccCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCtcaat                                                                                                                                                                                                             >  2:370267/1‑300 (MQ=255)                                                                                    ccccATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCtcaatca                                                                                                                                                                                                           >  1:491919/1‑300 (MQ=255)                                                                                    ccccATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACACCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTAAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCACGGTACGCCAAAAGCACAAACAACGCACAAGCCTGCTCGCACACTTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACACGTAAGCTGCGCAAAACGCAAAACAAGCtcaatca                                                                                                                                                                                                           >  2:140328/1‑300 (MQ=255)                                                                                        aTGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACt                                                                                                                                                                                                        <  1:680889/299‑1 (MQ=255)                                                                                                              ccATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCAGAAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTgatgagatga                                                                                                                                                                                 >  2:688475/1‑300 (MQ=255)                                                                                                              ccATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCACCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGAAAAAGAGTGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGTACAATTGAAAAAGCAGggagaa                                                                                                                                                                                                                                                                >  2:145057/1‑221 (MQ=255)                                                                                                                        ggCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGAt                                                                                                                                                                       >  2:96512/1‑300 (MQ=255)                                                                                                                                        agggttGCAATCATGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAAATTGATGAGATGAACGCGTCGATTGCCAAACTgg                                                                                                                                                            <  2:272937/291‑1 (MQ=255)                                                                                                                                         ggTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGAACAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCAACCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGAGTCGATTGCCAAACTGGagcagc                                                                                                                                                      >  1:16821/1‑300 (MQ=255)                                                                                                                                                          atcatcTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAANAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGNAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATTAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAg                                                                                                                                     <  2:547594/300‑1 (MQ=255)                                                                                                                                                             atcTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAgcgc                                                                                                                                  >  2:656557/1‑300 (MQ=255)                                                                                                                                                             atcTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAgcgc                                                                                                                                  <  1:344181/300‑1 (MQ=255)                                                                                                                                                                tACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAg                                                                                                                                <  2:443779/299‑1 (MQ=255)                                                                                                                                                                tACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACACTCAATCAACTCAATAAACAGATTGATTAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAg                                                                                                                                <  1:33927/299‑1 (MQ=255)                                                                                                                                                                      aGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTcgc                                                                                                                          <  1:545025/299‑1 (MQ=255)                                                                                                                                                                             ttAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGNGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAAc                                                                                                                  <  2:571049/300‑1 (MQ=255)                                                                                                                                                                                  gcTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGAt                                                                                                             <  1:73515/300‑1 (MQ=255)                                                                                                                                                                                    tGGCGTATTGTTGTGCGCCTTTTCCGCCCGCGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGTAAGAAGCGATCTCTTAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATTAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGc                                                                                                           <  2:386153/300‑1 (MQ=255)                                                                                                                                                                                    tGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGc                                                                                                           <  1:74361/300‑1 (MQ=255)                                                                                                                                                                                    tGGCGTATTGTTGTGCGCCTTTGCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTGTTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATTAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGc                                                                                                           <  2:437421/300‑1 (MQ=255)                                                                                                                                                                                     ggCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGcc                                                                                                          >  1:390547/1‑300 (MQ=255)                                                                                                                                                                                      gCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGTGATCTCTGAAGCCAACCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATGAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCACACAGGAGTGCAGCCTCGCCGCACAACTGGAt                                                                                                             >  2:86419/1‑296 (MQ=255)                                                                                                                                                                                        gTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCa                                                                                                       >  1:639096/1‑300 (MQ=255)                                                                                                                                                                                             gttgtGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTcc                                                                                                   >  1:634752/1‑299 (MQ=255)                                                                                                                                                                                             gttgtGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAAATGGATGCCGCATTcc                                                                                                   >  2:26413/1‑299 (MQ=255)                                                                                                                                                                                               tgtgCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTc                                                                                                <  1:555682/300‑1 (MQ=255)                                                                                                                                                                                                                       aTGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAGCATACCGGTATTCAGc                                                                        <  1:238063/300‑1 (MQ=255)                                                                                                                                                                                                                         gAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAGCATACCGGTATTCAGCTg                                                                      <  1:449574/300‑1 (MQ=255)                                                                                                                                                                                                                          aGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAAGTGGATGCCGCATTCCGTCAGGGCGAGCATACCGGTATTCAGCTGa                                                                     >  2:611926/1‑300 (MQ=255)                                                                                                                                                                                                                          aGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAGCATACCGGTATTCAGCTGa                                                                     >  1:689371/1‑300 (MQ=255)                                                                                                                                                                                                                          aGCGTGAACAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCCACCACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCTAGGAGCGCAGCCTCGCAGCACCACTGGATGCCGCATTCCGTCAGGGCGAGAATACCGGTATTCAGCTGa                                                                     >  1:689381/1‑300 (MQ=255)                                                                                                                                                                                                                             gTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAGCATACCGGTATTCAGCTGATtc                                                                  <  1:368239/300‑1 (MQ=255)                                                                                                                                                                                                                                   aaCTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATTAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGc                                                             <  1:305295/299‑1 (MQ=255)                                                                                                                                                                                                                                   aaCTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGc                                                             <  1:207318/299‑1 (MQ=255)                                                                                                                                                                                                                                              aTTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGc                                                 >  1:662963/1‑300 (MQ=255)                                                                                                                                                                                                                                                   ggCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAAAGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGcc                                                >  2:395683/1‑296 (MQ=255)                                                                                                                                                                                                                                                        aCATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGAc                                       <  1:68532/300‑1 (MQ=255)                                                                                                                                                                                                                                                        aCATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGAc                                       <  2:319292/300‑1 (MQ=255)                                                                                                                                                                                                                                                            cgccgcAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGc                                    >  1:382063/1‑299 (MQ=255)                                                                                                                                                                                                                                                             gccgcAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGt                                  <  1:636457/300‑1 (MQ=255)                                                                                                                                                                                                                                                                   aaaGAGCGCGCGGTACGCCAAAAGCAACAACAACGCACAAGCCTTCTCGCACAATTGAAAAAGCAGGAATAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAGAACACGCTCAATCAACGCAATAAACAGATTGATTAGATGAACGCGTCGATTTCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAg                            <  2:386563/300‑1 (MQ=255)                                                                                                                                                                                                                                                                            gcgGTACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCACACAACTGGATGCCGCATTCCGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTAttt                    <  2:465939/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                tACGCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGc                <  2:16821/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                   gCCAAAAGCAACAACAACGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTAtc            >  1:175372/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                              caacaacGCACAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGgc  >  2:720661/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                  GCGCCGACCATGTTGTCGCTGATGGGTATGGAAATCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGC  >  NC_000913/3786727‑3787310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑