Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,666,149	C→T	intergenic (‑339/+31)	gadX ← / ← gadA	DNA‑binding transcriptional dual regulator GadX/glutamate decarboxylase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,666,149	0	C	T	97.8%	277.7 / NA	90	intergenic (‑339/+31)	gadX/gadA	DNA‑binding transcriptional dual regulator GadX/glutamate decarboxylase A
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (34/53);  minor base G (0/2);  total (35/55)
Fisher's exact test for biased strand distribution p-value = 5.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAATGTGTGATGTTCTACGGGCAGGATGACTGGATTTATAATACAAAAACGTGTTTAAGAACACAGGGAAATTAATAGTAAGCAAATAATCCCTTTTCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGCTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCGTACAGGGTGTATCCCGGATCTTCAC  >  NC_000913/3665865‑3666427                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                       ctctTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAATGTGTGATGTTCTACGGGCAGGATGACTGGATTTATAATACAAAAACGTGTTTAAGAACACAGGGAAATTAATAGTAAGCAAATAATCCCTTTTCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGtttt                                                                                                                                                                                                                                                                         >  1:227672/1‑300 (MQ=255)  tctTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAATGTGTGATGTTCTACGGGCAGGATGACTGGATTTATAATACAAAAACGTGTTTAAGAACACAGGGAAATTAATAGTAAGCAAATAATCCATTTTCGTGACATTAAAGGTAATCGCTAAATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGtttt                                                                                                                                                                                                                                                                         >  2:66441/1‑299 (MQ=255)   ctTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAATGTGTGATGTTCTACGGGCAGGATGACTGGATTTATAATACAAAAACGTGTTTAAGAACACAGGGAAATTAATAGTAAGCAAATAATCCCTTTTCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTa                                                                                                                                                                                                                                                                       >  1:628176/1‑300 (MQ=255)              ttgattgaTAAATAAATTTAAACGAAGCGGTAGAACAGGGTGATAATGTTTGATGTTCTACGGGCAGGATGACTGGATTTATAATACAAAAACGTGTTTAAGAACACATGGAAATTAATAGTAAGCAAATAATCCCTTTTCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACgttatgt                                                                                                                                                                                                                                                            <  2:196556/300‑1 (MQ=255)                   tgatAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAATGTGTGATGTTCTACGGGCAGGATGACTGGATTTATAATACAAAAACGTGTTTAAGAACACAGGGAAATTAATAGTAAGCAAATAATCCCTTTTCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCa                                                                                                                                                                                                                                                       <  2:473021/300‑1 (MQ=255)                     ataaataaATTTAAACGAAGCGGGAGAACAGGATGATAATGTGTGATGTTCTACGGGCAGGATGACTGGATTTATAATACAAAAACGTGTTTAAGAACACAGGGAAATTAATAGTAAGCAAATAATCCCTTTTCGTGACATTAAAGGTAATCGCTACATTTAATNAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAgg                                                                                                                                                                                                                                                     <  2:521167/300‑1 (MQ=255)                     ataaataaATTTAAACGAAGCGGGAGAACAGGATGATAATGTGTGATGTTCTACGGGCAGGATGACTGGATTTATAATACAAAAACGTGTTTAAGAACACAGGGAAATTAATAGTAAGCAAATAATCCCTTTTCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAgg                                                                                                                                                                                                                                                     <  2:192970/300‑1 (MQ=255)                     ataaataaATTTAAACGAAGCGGGAGAACAGGATGATAATGTGTGATGTTCTACGGGCAGGATGACTGGATTTATAATACAAAAACGTGTTTAAGAACACAGGGAAATTAATAGTAAGCAAATAATCCCTTTTCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAgg                                                                                                                                                                                                                                                     <  1:278193/300‑1 (MQ=255)                      taaatagaTTTAAACGAAGCGGGAGAACAGGATGATAATGTTTGATGTTCTACGGGCAGGATGACTGGGTTTATAATACAAAAACGTGTTTAAGAACACAGGGAAATTAATAGTAAGCAAATAATCCCTTTTCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTGTTTACAACGTTATGTTATCAgg                                                                                                                                                                                                                                                     <  2:714813/299‑1 (MQ=255)                                tAAACGAAGCGGGAGAACAGTATGAGAATGTGTTATGTTCTACGTGCAGGATGACTGGATTTATAATACAAAAACGTGTTTAAGAACACATGGAAATTAAGAGTAAACAAATAATCCCTTTTCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTTATATAACATATATCTTATCAACACGATGAATAGACAGCTGATATATTATTGCGATTAA‑T‑A‑AGCAACCAAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGTGCATTCGTTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAg                                                                                                                                                                                                                                          <  2:132902/300‑1 (MQ=255)                                 aaaCGAAGCGGGAGAACAGGATGATAATGTTTGATGTTCTACGGGCAGGATGACTGGATTTATAATACAAAAACGTGTTTAAGAACACAGGGAAATTAATAGTAAGCAAATAATCCCTTTTCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGc                                                                                                                                                                                                                                         <  1:568433/300‑1 (MQ=255)                                         cGGGAGAACAGGATGANAATGTGTGATGTTCTACGGGCAGGATGACTGGATTTATAATACAAAAACGTGTTTAAGAACACAGGGAAATTAATAGTAAGCAAATAATCCCTTTTCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACNACGTTATGTTATCAGGTGNGTTTAAAGCTGTTctgc                                                                                                                                                                                                                                 >  1:609426/1‑300 (MQ=255)                                          gggAGAACAGGATGATAATGTGTGATGTTCTACGGGCAGGATGACTGGATTTATAATACAAAAACGTGTTTAAGAACACAGGGAAATTAATAGTAAGCAAATAATCCCTTTTCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTctgc                                                                                                                                                                                                                                 <  1:405107/299‑1 (MQ=255)                                           ggAGAACAGGATGATAATGTGTGATGTTCCACGGGCAGGATGACTGGATTTATAATACAAAAACGTGTTTAAGAACACAGGGAAATTAATAGTAAACAAATAATCCCTTTTCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTAATATCACATATATCTTATCAACACGATGAATAGAAAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCAAATGCCCAG‑C‑TGTTTTT‑TTAGAGGTTGGGCATTCGGTTTTTATAACGTTATGTTATCAGgt                                                                                                                                                                                                                                                    >  2:126699/1‑279 (MQ=255)                                              gaACAGGATGATAATGTGTGATGTTCTACGGGCAGGATGACTGGATTTATAATACAAAAACGTGTTTAAGAACACAGGGAAATTAATAGTAAGCAAATAATCCCTTTTCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGc                                                                                                                                                                                                                            <  2:585626/300‑1 (MQ=255)                                              gaACAGGATGATAATGTGTGATGTTCTACGGGCAGGATGACTGGATTTATAATACAAAAACGTGTTTAAGAACACAGGGAAATTAATAGTAAGCAAATAATCCCTTTTCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGc                                                                                                                                                                                                                            >  1:649922/1‑300 (MQ=255)                                                 cAGGATGATAATGTGTGATGTTCTACGGGCAGGATGACTGGATTTATAATACAAAAACGTGTTTAAGAACACAGGGAAATTAATAGTAAGCAAATAATCCCTTTTCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAAt                                                                                                                                                                                                                         >  1:355626/1‑300 (MQ=255)                                                   ggATGATAATGTGTGATGTTCTACGGGCAGGATGACTGGATTTATAATACAAAAACGTGTTTAAGAACACAGGGAAATTAATAGTAAGCAAATAATCCCTTTTCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATAc                                                                                                                                                                                                                       >  1:23399/1‑300 (MQ=255)                                                   ggATGATAATGTGTGANGTTCTACGGGCAGGATGACTGGATTTATAATACAAAAACGTGTTTAAGAACACAGGGAAATTAATAGTAAGCAAATAATCCCTTTTCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACGACGTTATGTNATCAGGTGTGTTTAAAGCNGTTCTGCTGGGCAATAc                                                                                                                                                                                                                       >  1:611647/1‑300 (MQ=255)                                                     atgatAATGTGTGATGTTCTACGGGCAGGATGACTGGATTTATAATACAAAAACGTGTTTAAGAACACAGGGAAATTAATAGTAAGCAAATAATCCCTTTTCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATAccc                                                                                                                                                                                                                     <  2:217234/300‑1 (MQ=255)                                                     atgatAATGTGTGATGTTCTACGGGCAGGATGACTGGATTTATAAGACAAAAACGTGTTTAAGAACACAGGGAAATTAATAGTAAGCAAANAATCCCTTTTCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATAccc                                                                                                                                                                                                                     <  2:583175/300‑1 (MQ=255)                                                       gatAATGTGTGATGTTCTACGGGCAGGATGACTGGATTTATAATACAAAAACGTGTTTAAGAACACAGGGAAATTAATAGTAAGCAAATAATCCCTTTTCGTGACATTAAAGGTAATCACTAAATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTCTGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCt                                                                                                                                                                                                                    >  2:363691/1‑299 (MQ=255)                                                        atAATGTTTGATGTTCTACGGGCAGGATGACTGGATTTATAATACAAAAACGTGTGTAAGAACACAGGGAAATTAATAGTAAGCAAATAATCCCTTTTCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGGTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGc                                                                                                                                                                                                                  <  1:354010/300‑1 (MQ=255)                                                                      tCTACGGGCAGGATGACTGGATTTATAATACAAAAACGTGTTTAAGAACACAGGGAAATTAATAGTAAGCAAATAATCCCTTTTAGTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGTGATTAA‑T‑G‑AGCAAACGAATGTACAG‑C‑TGTTTTT‑TTAAAGGTTGGGTATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATc                                                                                                                                                                                                    >  2:22845/1‑300 (MQ=255)                                                                      tCTAAGGGCAAGATGACTGGATTTATAATACAAAAACGTGTTTAAGAACACAGGGAAATTAATAGCAAGCAAATAATCCCTTTTCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTAATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATACTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCAAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTAAAACGTTATGTTATCAGGTGTGTTTAACGCTGTTCTCCTAGGCAATAACCTGTAGGTTCGGGTGAt                                                                                                                                                                                                     >  2:264849/1‑299 (MQ=255)                                                                                ggATGACTGGATTTATAATACAAAAACGTGTTTAAGAACACAGGGAAATTAATAGTAAGCAAATAATCCCTTTTCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGatat                                                                                                                                                                                          >  1:26567/1‑300 (MQ=255)                                                                                                     aaaaaCGTGTTTAAGAACACAGGGAAATTAATAGTAAGCAAATAATCCCTTTTCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTc                                                                                                                                                                      <  1:31783/299‑1 (MQ=255)                                                                                                       aaaCGTGTTTAAGAACACATAGAAATTAATAGTAAACAAATAATCCCTCTTCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACATACATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCNAG‑C‑TGTTTTT‑TTAAAGTTTGGGCATTCGGTTTNTACAACGTTATGTTATCAGGTGTGTGTAAAGCTATTCTGCGGGGTAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTc                                                                                                                                                                   <  2:551318/300‑1 (MQ=38)                                                                                                       aaaCGTGTTGAAGAACACAGGGAAATTAATAGTAAGCAAATAATCCCTTTTCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCNAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTNTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTTAGATATTTCAGGGAGGCTTTGTAGTCTTc                                                                                                                                                                   <  2:551314/300‑1 (MQ=255)                                                                                                              tttAAGAACACAGGGAAATTAATAGTAAGCAAATAATCCCTTTTCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAAc                                                                                                                                                            <  1:92118/300‑1 (MQ=255)                                                                                                              tttAAGAACACAGGGAAATTAATAGTAAGCAAATAATCCCTTTTCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATAGAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAAc                                                                                                                                                            <  2:447255/300‑1 (MQ=255)                                                                                                                tAAGAACACAGGGAAATTAATAGTAAGCAAATAATCCCTTTTCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACa                                                                                                                                                           >  1:102482/1‑299 (MQ=255)                                                                                                                   gAACACAGGGAAATTAATAGTAAGCAAATAATCCCTTTTCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTc                                                                                                                                                       <  2:467808/300‑1 (MQ=255)                                                                                                                         ggggAAATTAATAGTAAGCAAATAATCCCTTTTCTTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATGTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCaa                                                                                                                                                  <  2:18111/298‑1 (MQ=255)                                                                                                                                    tAGTAAGCAAATAATCCCTTTTCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACATATATCTTANCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTGAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAAcctgtctcttatacacatctcgc                                                                                                                                      <  1:150017/300‑23 (MQ=38)                                                                                                                                           cAAATAATCCCTTTTCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCcgc                                                                                                                                >  1:219024/1‑299 (MQ=255)                                                                                                                                               tcatCCCTTTTCGTGACATTAAAAGTAATCGCTACATTTAATAAACCTTCATATACCATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGCTGGGCATTCGGTTTTTACAACGTGATGTTATCAGGTGTGTTTCACGCTGTTCTGCTGGGCAATACTCTGCATTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCATCAGTTCAGCAAAGTCGATTTAGAAGCCGcaacga                                                                                                                           >  2:590232/3‑300 (MQ=37)                                                                                                                                                  tCCCTTTTCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTGTT‑TGAAAGGTTGGGCATTCGGTTTTTACAACGTTACGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTTAGATATTTCAGGTAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGacac                                                                                                                        <  1:730275/300‑1 (MQ=255)                                                                                                                                                      ttttCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGTTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTTAGATATTTCATGTAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACAt                                                                                                                      <  2:406574/298‑1 (MQ=255)                                                                                                                                                        ttCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGNCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATg                                                                                                                  <  1:534350/300‑1 (MQ=255)                                                                                                                                                         tcttGACATTAAAGGTAATCGCTACATTTAAGAAACATTCATAGAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATgc                                                                                                                 <  2:165993/297‑1 (MQ=255)                                                                                                                                                         tCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATgc                                                                                                                 <  1:658219/300‑1 (MQ=255)                                                                                                                                                           gTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATgcgc                                                                                                               <  1:246646/300‑1 (MQ=255)                                                                                                                                                           gTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATgcgc                                                                                                               <  2:623503/300‑1 (MQ=255)                                                                                                                                                           gTGACATTAAAGGTAATCGCTACATTGAATAAACATGCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTTAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATgcgc                                                                                                               <  1:246627/300‑1 (MQ=255)                                                                                                                                                            tGACATTAAAGGTAATCGCTACAGTTAATAAACAGTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTAGTGCGATTAA‑T‑A‑AGCAACCGAATACCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATgc                                                                                                                 <  1:658262/297‑1 (MQ=255)                                                                                                                                                               cATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATca                                                                                                           >  1:686363/1‑300 (MQ=255)                                                                                                                                                                           tCGCTACATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTc                                                                                                 >  2:624257/1‑298 (MQ=255)                                                                                                                                                                           tCGCTAAATTTAATAAACATTCATATAACATATATCTTATCAAAACGATGAATAGACAGCCAATATATTATTGCGATTCA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTCAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTGTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTAGGGTGATCGCTAAGATATTTCAGGGAGGCTTTGTAGTCTTCCCGCAACCGTTCAGCAAAGTCCCTTTCGAAGCCGCGACGacac                                                                                                                        >  2:135851/1‑275 (MQ=255)                                                                                                                                                                               tACATTTGATAAACATTCATAGAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑NGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGATGTGCAATACCCTGCAGTTTCGGGTGATCGCTTAGATATTTCATGGAGGCTTTTTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTgtc                                                                                           <  1:147517/300‑3 (MQ=25)                                                                                                                                                                                aCATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCt                                                                                          >  1:58823/1‑300 (MQ=255)                                                                                                                                                                                     taataaACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCAcc                                                                                     <  2:628176/300‑1 (MQ=255)                                                                                                                                                                                       ataaACATTCATATAACATATATCTGATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTTAGATATTTCAGGGAGGTTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCAccgc                                                                                   <  2:397057/300‑1 (MQ=255)                                                                                                                                                                                       agaaaCATTCATATGACAGATATCCTAGCAACACTATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGTGTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATGTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCACTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCAccgc                                                                                   <  2:280558/298‑1 (MQ=21)                                                                                                                                                                                                cctatcacctatatCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGa                                                                          >  1:287050/10‑300 (MQ=255)                                                                                                                                                                                                   ataACATATATCTTAGCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAgg                                                                       <  1:94521/300‑1 (MQ=255)                                                                                                                                                                                                      aCATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTCGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGcc                                                                     >  2:473341/1‑299 (MQ=255)                                                                                                                                                                                                      aCATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGcc                                                                     >  2:702193/1‑299 (MQ=255)                                                                                                                                                                                                      aCATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCAGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGTTGTTCTGCTGGGCAATACCATGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATCTCGAAGCCGCGACGACACATAATGCGCATCAACACGATGTCGGTGGCTTCACTGCCGAGAGTGAAGGcc                                                                     >  2:408950/1‑299 (MQ=255)                                                                                                                                                                                                       cATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAgg                                                                                                                                                                                                                                                     >  1:709038/1‑122 (MQ=255)                                                                                                                                                                                                       cATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAgg                                                                                                                                                                                                                                                     >  1:709031/1‑122 (MQ=255)                                                                                                                                                                                                       cATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAgg                                                                                                                                                                                                                                                     <  2:709031/122‑1 (MQ=255)                                                                                                                                                                                                       cATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAgg                                                                                                                                                                                                                                                     <  2:709038/122‑1 (MQ=255)                                                                                                                                                                                                       cATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAANGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGcc                                                                     >  1:141793/1‑298 (MQ=255)                                                                                                                                                                                                                  tcgacacGATTAATAGACATCCAATATATTACTTCGATTAA‑T‑A‑AGCAACCGAATTCCCAG‑C‑TGTTTTT‑TTAAAGGTTGTGCATTCGGTTTTTACAACGTTATGTTATCATGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGTGTGATCGCTTAGATATTTCATGTAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCTACGACACATAATGCGCACCACCACGATGTCGGTGGCTTTACCGCCGAGAGTGAAGGCCGGAACCTGCCAGc                                                        <  2:111762/297‑1 (MQ=18)                                                                                                                                                                                                                  tCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGTTTGTGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCATGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGc                                                        <  2:112106/300‑1 (MQ=255)                                                                                                                                                                                                                  tCAACACAATGAATAGACAGCCAATATATTAGTGCGATTAA‑T‑A‑AACAACCGAAGGCCCAG‑C‑TGTTTTT‑TTAAAGGGTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGTTGATCGCTTAGATATTTCAGGGAGGCTTTTTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCTAAGCCTCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTTACCGCCGAGAGTGAAGGCCGGAACCTGCCAGc                                                        <  2:111541/300‑1 (MQ=18)                                                                                                                                                                                                                       acgctGAATAAACAGCCAGTGCATTATTTCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTTTTCTGCTTGGCAATACCCTACAGTTGCGGGTGATCGCTGAGATATTTCAGGGATGTTGTGTAGTTTTCCGGCAACGGTGCAGCAGAGTTCATTTCGAAGCCTCGACGACACATAATGCGCATCACCACGATGGCGGTGGCTTTGCCGCCGAGAGTGAAGGc                                                                      <  2:76198/277‑1 (MQ=255)                                                                                                                                                                                                                         gATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCCGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGCCGcgca                                                  >  1:264002/1‑299 (MQ=255)                                                                                                                                                                                                                         gATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGCCGcgca                                                  >  1:263919/1‑299 (MQ=255)                                                                                                                                                                                                                                    aGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCAAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGTGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTTAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGCCGCGCAGACGCAGACGt                                       <  2:58823/299‑1 (MQ=255)                                                                                                                                                                                                                                     gCCAATATATTATTGCNATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGANGGCCGGAACCTGCCAGCCNCGCAGACGCAGACGTTc                                     >  1:620530/1‑300 (MQ=255)                                                                                                                                                                                                                                     gCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGCCGCGCAGACGCAGACGTTc                                     >  1:400332/1‑300 (MQ=255)                                                                                                                                                                                                                                     gCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCAAGCCGCGCAGACGCAGACGtt                                      >  1:400943/1‑299 (MQ=255)                                                                                                                                                                                                                                     gCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCAGAGAGTGAAGGCCGGAACCTGCCAGCCGCGCAGACGAAGACGTTc                                     >  1:401087/1‑300 (MQ=255)                                                                                                                                                                                                                                     gCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCAACACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGCCGCGCAGACGCAGACGTTc                                     >  2:631994/1‑300 (MQ=255)                                                                                                                                                                                                                                      ccAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGCCGCgcagacgcagac                                         <  2:644634/295‑1 (MQ=255)                                                                                                                                                                                                                                         atatatTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCAGAGAGTGAAGGCCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCaga                                  >  1:11134/1‑299 (MQ=255)                                                                                                                                                                                                                                           tatgcattGCGATTAA‑TNA‑AGCAACCGAATGCCCAGNC‑TGTTTTC‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGCCGCGCANACGCAGACGTTCagag                                 <  1:608933/295‑1 (MQ=255)                                                                                                                                                                                                                                           tatccattGCGATTAAGN‑A‑AGCAACCGAATGCCCAGNC‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGCCGCGCANACGCAGACGTTCagag                                 <  1:608523/295‑1 (MQ=255)                                                                                                                                                                                                                                             ttggctGCGATTAA‑T‑ANAGCAACCGAATGCCCAG‑CNTGTTTTTCTTAAAGGTTGGGCATTCGGTTTTTACAACTTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGCCGCGCANACGCAGACGTTCagag                                 <  1:607661/294‑1 (MQ=17)                                                                                                                                                                                                                                                       ttAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGTGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCACCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCGTACAGGgt                   <  1:513252/300‑1 (MQ=255)                                                                                                                                                                                                                                                       ttAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGTGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGNTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCGTACAGGgt                   <  1:158805/300‑1 (MQ=255)                                                                                                                                                                                                                                                       ttAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGTCGAGAGTGAAGGCCGGAACCTGCCAGCCGcgcagacgcagcctttcctctcgttcgtacagggt                   <  1:34/300‑25 (MQ=17)                                                                                                                                                                                                                                                        taa‑t‑a‑aGCAACCGAATACCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCGTACAGGgtg                  <  1:119480/300‑1 (MQ=255)                                                                                                                                                                                                                                                              a‑aGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCGTACAGGGTGTATc              <  1:346975/300‑1 (MQ=255)                                                                                                                                                                                                                                                                aGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCGTACAGGGTGTATcc             <  1:22845/300‑1 (MQ=255)                                                                                                                                                                                                                                                                       gAATGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTGAGGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTTAGATATTTCATGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCATAGAGGTCGTACAGGGTGTATCCCGGAt        <  2:306558/298‑1 (MQ=255)                                                                                                                                                                                                                                                                        aaTGCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCGTACAGGGTGTATCCCGGATc       <  1:126699/298‑1 (MQ=255)                                                                                                                                                                                                                                                                           gCCCAG‑C‑TGTTTTT‑TTAAAGGTTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCCGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCGTACAGGGTGTATCCCGGATCTTCAc  <  1:66441/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                       CTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAATGTGTGATGTTCTACGGGCAGGATGACTGGATTTATAATACAAAAACGTGTTTAAGAACACAGGGAAATTAATAGTAAGCAAATAATCCCTTTTCGTGACATTAAAGGTAATCGCTACATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAA‑T‑A‑AGCAACCGAATGCCCAG‑C‑TGTTTTT‑TTAAAGGCTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCGTACAGGGTGTATCCCGGATCTTCAC  >  NC_000913/3665865‑3666427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑