Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,159,997	A→T	L265Q (CTG→CAG)	sthA ←	soluble pyridine nucleotide transhydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,159,997	0	A	T	100.0%	271.6 / NA	76	L265Q (CTG→CAG)	sthA	soluble pyridine nucleotide transhydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (44/32);  total (44/32)

TCGGTTTTGCCCACAGAGCTGATTTCCGGGATGGTGTAAATACCGGTAGGGATATCTTCAATCAGATGTGCGGTGGCTTCGCCTTTTACCAGCGCCTGCGCGGCAATGCGCCCCTGGTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTC  >  NC_000913/4159703‑4160292                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                        tCGGTTTTGCCCACAGAGCTGATTTCCTGGATGGTGTAAATACCGGTAGGGATATCTTCAATCAGATGTGCGGTGGCTTCGCCTTTTACCAGCGCCTGCGCGGCAATGCGCCCCTGGTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTAACGGTGCGACCGTTGGCATAGAgctggcag                                                                                                                                                                                                                                                                                                    <  1:404137/300‑1 (MQ=255) tCGGTTTTGCCCACAGAGCTGATTTCCGGGATGGTGTAAATACCGGTAGGGATATCTTCAATCAGATGTGCGGTGGCTTCGCCTTTTACCAGCGCCTGCGCGGCAATGCGCCCCTGGTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAgctggcag                                                                                                                                                                                                                                                                                                    <  1:10099/300‑1 (MQ=255)                                  gtgtAAATACCGGTAGGGATATCTTCAATCAGATGTGCGGTGGCTTCGCCTTTTACCAGCGCCTGCGCGGCAATGCGCCCCTGGTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTCCCCGACTTCAGATGc                                                                                                                                                                                                                                                                   >  2:427398/1‑300 (MQ=255)                                  gtgtAAATACCGGTAGGGATATCTTCAATCAGATGTGCGGTGGCTTCGCCTTTTACCAGCGCCTGCGCGGCAATGCGCCCCTGGTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGc                                                                                                                                                                                                                                                                   >  1:129368/1‑300 (MQ=255)                                  gtgtAAATACCGGTAGGGATATCTTCAATCAGATGTGCGGTGGCTTCGCCTTTTACCAGCGCCTGCGCGGCAATGCGCCCCTGGTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGc                                                                                                                                                                                                                                                                   >  2:689412/1‑300 (MQ=255)                                  gtgtAAATACCGGTAGGGATATCTTCAATCAGATGTGCGGTGGCTTCGCCTTTTACCAGCGCCTGCGCGGCAATGCGCCCATGGTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGc                                                                                                                                                                                                                                                                   >  1:129427/1‑300 (MQ=255)                                    gtAAATACCGGTAGGGATATCTTCAATCAGATGTGCGGTGGCTTCGCCTTTTACCAGCGCCTGCGCGGCAATGCGCCCCTGGTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTAGCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCAt                                                                                                                                                                                                                                                                 >  2:487413/1‑300 (MQ=255)                                    gtAAATACCGGTAGGGATATCTTCAATCAGATGTGCGGTGGCTTCGCCTTTTACCAGCGCCTGCGCGGCAATGCGCCCCTGGTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCAt                                                                                                                                                                                                                                                                 >  2:487423/1‑300 (MQ=255)                                      aaaTACCGTTAGGGATATCTTCAATCAGATGTGCGGTGGCTTCGCCTTTTACCAGCGCCTGCGCGGCAATGCGCCCCTGTTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACATGTGGTTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGa                                                                                                                                                                                                                                                               <  2:350718/300‑1 (MQ=255)                                          aCCGGTAGGGATATCTTCAATCAGATGTGCGGTGGCTTCGCCTTTTACCAGCGCCTGCGCGGCAATGCGCCCCTGGTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTAGCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATcac                                                                                                                                                                                                                                                           <  1:487413/300‑1 (MQ=255)                                          aCCGGTAGGGATATCTTCAATCAGATGTGCGGTGGCTTCGCCTTTTACCAGCGCCTGCGCGGCAATGCGCCCCTGGTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATcac                                                                                                                                                                                                                                                           <  1:487423/300‑1 (MQ=255)                                           ccGGTAGGGATATCTTCAATCAGATGTGCGGTGGCTTCGCCTTTTACCAGCGCCTGCGCGGCAATGCGCCCCTGGTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCACAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATcaca                                                                                                                                                                                                                                                          >  2:324938/1‑300 (MQ=255)                                                            aaTCAGATGTGCGGTGGCTTCGCCTTTTACCAGCGCCTGCGCGGCAATGCGCCCCTGGTCATAGGCCGCCGCCGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTACCGCCAGCGAGTCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTc                                                                                                                                                                                                                                         >  1:179091/1‑300 (MQ=255)                                                                aGATGTGCGGTGGCTTCGCCTTTTACCAGCGCCTGCGCGGCAATGCGCCCCTGGTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGAtc                                                                                                                                                                                                                                     <  2:599714/300‑1 (MQ=255)                                                                aGATGTGCGGTGGCTTCGCCTTTTACCAGCGCCTGCGCGGCAATGCGCCCCTGGTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGAt                                                                                                                                                                                                                                      <  2:506168/299‑1 (MQ=255)                                                                 gATGTGCGGTGGCTTCGCCTTTTACCAGCGCCTGCGCGGCAATGCGCCCCTGGTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTCACGCCAGCGAATCGGTATTACTGGTGCGCCCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCAAAACATCGTCACAGCCTTCGAtct                                                                                                                                                                                                                                    >  2:374882/1‑300 (MQ=255)                                                                 gATGTGCGGTGGCTTCGCCTTTTACCAGCGCCTGCGCGGCAATGCGCCCCTGGTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGAtct                                                                                                                                                                                                                                    >  1:419474/1‑300 (MQ=255)                                                                           ggCTTCGCCTTTTACCAGCGCCTGCGCGGCAATGCGCCCCTGGTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTActc                                                                                                                                                                                                                          <  1:81927/300‑1 (MQ=255)                                                                                  ccTTTTACCAGCGCCTGCGCGGCAATGCGCCCCTGGTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTActc                                                                                                                                                                                                                          <  1:8236/293‑1 (MQ=255)                                                                                  ccTTTTACCAGCGCCTGCGCGGCAATGCGCCCCTGGTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTActc                                                                                                                                                                                                                          >  2:8236/1‑293 (MQ=255)                                                                                  ccTTTTACCAGCGCCTGCGCGGCAATGCGCCCCTGGTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGtt                                                                                                                                                                                                                    >  1:703108/1‑299 (MQ=255)                                                                                                  gcgcGGCAATGCGCCCCTGGTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGAATTCAGATGCATGATCCCACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGc                                                                                                                                                                                                   >  2:361566/1‑300 (MQ=255)                                                                                                  gcgcGGCAATGCGCCCCTGGTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCACAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGAACGTTGGCATAGAGCTGGCAGTCGGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACTCTCGTCACAGCCTTCGATCTTCTAGTACTCTTCGTTGTGACGAATCACTaagc                                                                                                                                                                                                   >  2:361357/1‑297 (MQ=255)                                                                                                  gcgcGGCAATGCGCCCCTGGTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCAACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGc                                                                                                                                                                                                   <  1:591323/300‑1 (MQ=255)                                                                                                    gcgGCAATGCGCCCCTTGTCATAGGCCGCCGACGCCAGGCTCGGAGAACCAATCACGTCGCCCACCGCGTAAACGTTTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCTGCTGTCAGTTTCTAGCCCAATGTTCTTTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGc                                                                                                                                                                                                   <  2:313825/298‑1 (MQ=255)                                                                                                         aaTGCGCCCCTGGTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGtt                                                                                                                                                                                            >  1:694885/1‑300 (MQ=255)                                                                                                           tGCGCCCCTGGTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTcc                                                                                                                                                                                          <  2:672054/300‑1 (MQ=255)                                                                                                                 ccTGGTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGNCCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTATTCTTCGTTGTGACGAATCACTAAGCCACTGTTCCAGAAGt                                                                                                                                                                                    >  2:221996/1‑300 (MQ=255)                                                                                                                   tGGTCATAGGCCGCCGACGCCAGGCTAGGATAACCAATCACGTCGCCCACCGCGGAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAg                                                                                                                                                                                     <  2:183060/297‑1 (MQ=255)                                                                                                                     gTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAANGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTAGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCAAGAAGTGATa                                                                                                                                                                                >  2:522433/1‑300 (MQ=255)                                                                                                                     gTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATa                                                                                                                                                                                >  1:460229/1‑300 (MQ=255)                                                                                                                     gTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATa                                                                                                                                                                                >  2:321389/1‑300 (MQ=255)                                                                                                                     gTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTAGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATa                                                                                                                                                                                >  2:144767/1‑300 (MQ=255)                                                                                                                     gTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTAGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATa                                                                                                                                                                                >  1:61972/1‑300 (MQ=255)                                                                                                                          aGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGgag                                                                                                                                                                            <  2:421167/299‑1 (MQ=255)                                                                                                                                    cGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGa                                                                                                                                                                 >  1:221955/1‑300 (MQ=255)                                                                                                                                             cGGATAACCAATCACGTCGCCCACCGCGTAAATGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTCCCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTAAGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCtt                                                                                                                                                         >  2:387928/1‑299 (MQ=255)                                                                                                                                                   aCCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTAGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTATTGATCga                                                                                                                                                   >  2:668791/1‑299 (MQ=255)                                                                                                                                                    ccAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTAGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCAAGAAGTGATAGGAGAGAGAATCTGACATCTATTGATCga                                                                                                                                                   >  2:268824/1‑298 (MQ=255)                                                                                                                                                                                 tgGCTGTTCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGTAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGAt                                                                                                                    <  1:247192/300‑1 (MQ=255)                                                                                                                                                                                 tgGCTGTGCGGTCTGATNCATGCTGTTGACCTTCAGNTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGNGATCGCGGGTGTTGAt                                                                                                                    <  1:606827/300‑1 (MQ=255)                                                                                                                                                                                  ggCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATc                                                                                                                   <  1:109213/300‑1 (MQ=255)                                                                                                                                                                                           gTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCACAATGTTCTGGAACGCAAGCGAATCGGTATTACCGGTGCGACCGTTGGCAAAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTAGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCAc                                                                                                           >  2:640460/1‑299 (MQ=255)                                                                                                                                                                                           gTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCCAGCACAATGTTCTGAAACGCCAGCGAATCGGTATTAACGGTGCGACCGTTGGCAAAGAGCTGGCAGTCAGCTTTCAGTTTTTTAACCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGCATCACTAAGCCAATGTTCAAGAAGTGGTAGGGGAGAGAATCTGACATCTATTGATCGAGAAATGCCAGCAGGCGATCGCGGgtgt                                                                                                                        >  2:640204/1‑286 (MQ=255)                                                                                                                                                                                               gATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTa                                                                                                      >  1:53480/1‑300 (MQ=255)                                                                                                                                                                                               gATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTATTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACtttt                                                                                                       >  1:53829/1‑299 (MQ=255)                                                                                                                                                                                               gATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCAAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTa                                                                                                      >  1:308124/1‑300 (MQ=255)                                                                                                                                                                                                    aTGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTTTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCNATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATcc                                                                                                 <  2:520472/300‑1 (MQ=255)                                                                                                                                                                                                    aTGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTTTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATc                                                                                                  <  1:493990/299‑1 (MQ=255)                                                                                                                                                                                                       cTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATa                                                                                              >  1:26712/1‑300 (MQ=255)                                                                                                                                                                                                        tGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATAc                                                                                             <  1:223864/300‑1 (MQ=255)                                                                                                                                                                                                                                tGTCAGTGTCTAGCCCAATGTGTTGTAACGCCAGCGAATTGGTATTACCGGTGCGACTGTTGGCGTAGAGCTGGCAGTTAGCTTTCAGTTTTTTACCCGACTTCAGATGCAGGATCACACCATCGTCACAGCCTTCGATCTTCTCATACTCTTCGTTGTGACGAATCACTACGCCACTGTGCCAGAAGTGATAGGATAGAGAATCTGACATCTCTTGATCGGGAAATGCCAGCATGCGATCGCGGGTGTTGATCAGATCCACTTTGACATCCATACCGCGGAAGATCGACGCATATTcac                                                                     <  2:208600/300‑1 (MQ=255)                                                                                                                                                                                                                                           aGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACt                                                          >  2:158126/1‑300 (MQ=255)                                                                                                                                                                                                                                                  tGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCAc                                                   <  2:701774/300‑1 (MQ=255)                                                                                                                                                                                                                                                  tGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCAc                                                   <  1:427398/300‑1 (MQ=255)                                                                                                                                                                                                                                                   gTTCTGTAACGCAAGCGAGGCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTGTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGTAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCAc                                                   <  2:179091/299‑1 (MQ=255)                                                                                                                                                                                                                                                      cTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCAt                                                >  1:387322/1‑299 (MQ=255)                                                                                                                                                                                                                                                        gTAACGCCAGCGAATCGGGATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGTATAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGa                                             <  2:296506/300‑1 (MQ=255)                                                                                                                                                                                                                                                         tAACGCCAGCGAATCGTTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCATAATTGATAGGATAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGa                                             <  2:297323/299‑1 (MQ=255)                                                                                                                                                                                                                                                          aaCGCCAGTGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCGGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATa                                           <  2:129368/300‑1 (MQ=255)                                                                                                                                                                                                                                                          aaCGCCAGCTAATCGGTACTACCATTGCGACCGTTGGCAGAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGGACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGTAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGa                                             <  2:296173/298‑1 (MQ=255)                                                                                                                                                                                                                                                          aaCGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCTAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCAATCACTCCAGCACCATAGATa                                           <  2:129427/300‑1 (MQ=255)                                                                                                                                                                                                                                                          aaCGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATa                                           <  1:509945/300‑1 (MQ=255)                                                                                                                                                                                                                                                                aGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTAc                                      <  1:625314/299‑1 (MQ=255)                                                                                                                                                                                                                                                                    aaTCGGTGTTACCGGGGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCATAAGTGATAGGATAGAGAATCTGACACCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAAATAAGTACATGgc                                 <  2:439218/300‑1 (MQ=255)                                                                                                                                                                                                                                                                         gTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGt                            >  1:363238/1‑300 (MQ=255)                                                                                                                                                                                                                                                                         gTATTACCGGTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTAGTACTCTTCGTTGTGACGAATCACTACGCCANTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGt                            >  2:540857/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                  gTGCGACCGTTGGCATAGAGTTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTATTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCCTACCGCGGAAGATCGACGCATATTCACAGCCAATCACTCCAGCCCCATAGATAAGTACATGGCGAGGTTCgtggt                     >  2:313732/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                  gTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGTAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGc                   <  1:361357/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                  gTGCGACCGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGc                   <  1:361566/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                         cGTTGGCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTga            >  2:642382/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                             ggCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGATAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGANGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAAt        >  1:146782/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                             ggCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGANGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAAt        >  1:147515/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                             ggCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAAt        >  1:257092/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                              gCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAAtt       >  1:74015/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                              gCATAGAGCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAAtt       >  2:319551/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                   gagCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTc  >  1:370228/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                   gagCTGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGAGGTTCGTGGTGCATGCTGAGAATTGAGTc  >  1:697250/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                        TCGGTTTTGCCCACAGAGCTGATTTCCGGGATGGTGTAAATACCGGTAGGGATATCTTCAATCAGATGTGCGGTGGCTTCGCCTTTTACCAGCGCCTGCGCGGCAATGCGCCCCTGGTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTC  >  NC_000913/4159703‑4160292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑