Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,138,814	G→A	D74D (GAC→GAT)	gldA ←	L‑1,2‑propanediol dehydrogenase/glycerol dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,138,814	0	G	A	100.0%	325.7 / NA	94	D74D (GAC→GAT)	gldA	L‑1,2‑propanediol dehydrogenase/glycerol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (51/43);  total (51/43)

TCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTC  >  NC_000913/4138524‑4139108                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                       tCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCa                                                                                                                                                                                                                                                                                               >  1:570586/1‑300 (MQ=255)   gCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTACGCGATGCCACGCAGACGATCGATCTCAtt                                                                                                                                                                                                                                                                                             >  1:715302/1‑300 (MQ=255)              ccgcTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGTAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGACGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCAACGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGGCGATCGATCTCATTTTGCGAACAtt                                                                                                                                                                                                                                                                                  >  1:228105/1‑300 (MQ=255)                gcTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGTGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCa                                                                                                                                                                                                                                                                                >  1:24672/1‑300 (MQ=255)                  tAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCAcc                                                                                                                                                                                                                                                                              >  2:418670/1‑300 (MQ=255)                       gACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACCTTCACCGCCaa                                                                                                                                                                                                                                                                         >  2:482951/1‑300 (MQ=255)                       gACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCaa                                                                                                                                                                                                                                                                         >  1:172504/1‑300 (MQ=255)                          gTGCCGGTGCGCCAGCGACGATTTTGGTGTCGCCAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGACCATTCACCGCCAAAc                                                                                                                                                                                                                                                                       >  1:291516/1‑299 (MQ=255)                          gTGCAGGTGCGCCAGCGACGATTTTGGTGTCGCCAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTAACGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGNCACCGATACCGAGACTTGCGCCACACTGCGCAGTCTCCGCGCTGCCACGCAGACGATCGATCTCATTTTGCGAACATTCAc                                                                                                                                                                                                                                                                               >  2:568417/1‑291 (MQ=255)                          gTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAAc                                                                                                                                                                                                                                                                       >  1:291531/1‑299 (MQ=255)                                           acgaTTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTAcc                                                                                                                                                                                                                                                     <  1:132049/300‑1 (MQ=255)                                              aTTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGt                                                                                                                                                                                                                                                  >  1:515543/1‑300 (MQ=255)                                                         gACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCttt                                                                                                                                                                                                                                       >  1:181690/1‑300 (MQ=255)                                                              tGACCATATTCTGGTTATTTGGCAGCAGCAGATAGCGGTCAAACTCACCCTCATCGGTTTAGATAACAGACAATTCGCTGCACGGTGCATCGGTAGAGTCGATAGTCGGTGCGATCGCTACCGGAACACCCATTAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAg                                                                                                                                                                                                                                   <  2:175581/299‑1 (MQ=255)                                                                         gggTTATGTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGAc                                                                                                                                                                                                                        <  1:36325/299‑1 (MQ=255)                                                                                  ggCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGTTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTTCGAACATCCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTATCGACa                                                                                                                                                                                                                       >  2:296773/1‑291 (MQ=255)                                                                                   gaaaCAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGa                                                                                                                                                                                                              <  2:494459/297‑1 (MQ=255)                                                                                     aaCAGCAGATAGCGTTCAAACGCACCCTCATCGGTGTAGATAACATACAATGCGCTGCACGGTGCATCGGTAGATGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCGTCTTTAAAGCTTTTCTCGACAGTGGATTGAGc                                                                                                                                                                                                            <  2:195529/299‑1 (MQ=255)                                                                                     aaCAGCAGATAGCGGTCAAACTCACCCGCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGc                                                                                                                                                                                                            <  1:131986/299‑1 (MQ=255)                                                                                         gcagATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAAc                                                                                                                                                                                                       >  1:571131/1‑300 (MQ=255)                                                                                         gcagATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGAAAGTGGATTGAGCaaaa                                                                                                                                                                                                        >  2:475010/1‑299 (MQ=255)                                                                                                      aaaCTCACCCTCATCGGTTTAGATAACAGACAATGCGCTACACGGTGCAGCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAATATCGAGGTTTTTTCCACCACCAAGACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGc                                                                                                                                                                                                            <  2:184711/282‑1 (MQ=255)                                                                                                       aaCTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAAttt                                                                                                                                                                                          >  1:177286/1‑299 (MQ=255)                                                                                                       aaCTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAAttt                                                                                                                                                                                          >  2:687136/1‑299 (MQ=255)                                                                                                       aaCTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGAAAACGATCGATCTCATTTTGCGAACATTCACCGCCACACGGCGCAATTTCTAATACCAGTCCAGCATCTTTACAGCTTTTATCGAAAGTGGATTGAGCAGAACCTaa                                                                                                                                                                                                   >  2:172123/1‑290 (MQ=255)                                                                                                                 cATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACccacca                                                                                                                                                                               >  2:437308/1‑300 (MQ=255)                                                                                                                     ggTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTATAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTaa                                                                                                                                                                           <  1:202511/300‑1 (MQ=255)                                                                                                                      gtgtAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAAc                                                                                                                                                                          >  1:215672/1‑300 (MQ=255)                                                                                                                      gtgtAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTAACGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTAGATTGAGTAAAACCTAAAACAAATTTGTCACCCACCACTa                                                                                                                                                                            >  2:600907/1‑298 (MQ=255)                                                                                                                       tttAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATTAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACTGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAAcc                                                                                                                                                                         <  2:291531/298‑1 (MQ=255)                                                                                                                        gtAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACAGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCCAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTCAAGCTTTCCTGGGCAGTGGATTGGGCAAAAGATAAAACAAATCTGTcacccacaacgcaaca                                                                                                                                                                        >  2:382925/1‑291 (MQ=255)                                                                                                                        gtAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAAGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACTGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAATATTCACCGCCCAACGGTGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTAGACAGTGGATTGAGCAAAACCTAAAACACATTTGTTACccaccaaccacca                                                                                                                                                                        >  2:205058/1‑293 (MQ=255)                                                                                                                         tAGATAACAGACAATGCGCTGCACGGTGCATCGGTATAGGCGATAGTCGGTGCGATCGCTACTGGAACACCCATTAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCTCGGTGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAACTTGTCACCCACCGCTAAcc                                                                                                                                                                         <  2:291516/298‑1 (MQ=255)                                                                                                                          aGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCCGACGATCGATTTCATTTTGCGCACATTCACCGCCAAACGGCGCAATTTCTACTAACAGTCCAGCATCTTTCAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTaa                                                                                                                                                                           >  2:148843/1‑295 (MQ=255)                                                                                                                                      aTGCGCTGCACGGTGCATCGTTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATTAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCg                                                                                                                                                          <  2:352905/300‑1 (MQ=255)                                                                                                                                      aTGCGCTGCACGGTGCATCGGTAGAGTCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCg                                                                                                                                                          <  2:352968/300‑1 (MQ=255)                                                                                                                                        gcgcTGCACGGTGCATNGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAANTTGTCACCCACCACTAACNAGCGTTCTGCCAGCGGc                                                                                                                                                        >  1:604486/1‑300 (MQ=255)                                                                                                                                               acgatGCATCAGTAGAGGCGATAGTCGGTGCGATCACTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGNGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAgg                                                                                                                                                  <  2:239107/295‑1 (MQ=255)                                                                                                                                               aCGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTANCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAgg                                                                                                                                                  <  1:148843/299‑1 (MQ=255)                                                                                                                                                 ggTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTAt                                                                                                                                               <  1:684106/300‑1 (MQ=255)                                                                                                                                                  gTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTAtt                                                                                                                                              >  1:25131/1‑300 (MQ=255)                                                                                                                                                  gTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAAACTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTAtt                                                                                                                                              >  1:383213/1‑300 (MQ=255)                                                                                                                                                      aTCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATTAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACNGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGc                                                                                                                                           <  2:579239/299‑1 (MQ=255)                                                                                                                                                         ggTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCAACCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCa                                                                                                                                        >  1:184525/1‑299 (MQ=255)                                                                                                                                                         ggTAGAGGCGATAGTCGGTGC‑ATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGa                                                                                                                                      >  1:350990/1‑300 (MQ=255)                                                                                                                                                                     aGTCGTTGCGATCGCTACCGGAACACTCCTTAAGTGTTCCAATGCTTTTGCAGTATCGAGGTTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCACAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAATATTCACCGCCAAACGGCACAATTTCTACTACCAGTCCAGCATCTTTAAAGCTCTTCTCGACAGTGGATTGAGc                                                                                                                                                                                                            <  2:184968/219‑1 (MQ=255)                                                                                                                                                                              gATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAgcgc                                                                                                                   <  2:382324/299‑1 (MQ=255)                                                                                                                                                                                   cTACCGGAACACCCATGAAATGTGCAAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCAACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAAAATAAAACAAATTTGTCACccacca                                                                                                                                                                               >  2:305428/1‑234 (MQ=255)                                                                                                                                                                                     aCCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATg                                                                                                           <  2:465680/300‑1 (MQ=255)                                                                                                                                                                                         gAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTAtt                                                                                                       <  2:659445/300‑1 (MQ=255)                                                                                                                                                                                             acCCATTAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTAcc                                                                                                   <  1:300600/300‑1 (MQ=255)                                                                                                                                                                                              cccATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACNACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTAccc                                                                                                  >  1:525719/1‑300 (MQ=255)                                                                                                                                                                                              cccATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTAccc                                                                                                  >  1:375605/1‑300 (MQ=255)                                                                                                                                                                                              cccATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTAATACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCAAATCAGCGCCCTGGATGTATTTAccc                                                                                                  >  1:375617/1‑300 (MQ=255)                                                                                                                                                                                                         tgCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGaat                                                                                       <  1:240782/300‑1 (MQ=255)                                                                                                                                                                                                                gCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACCTTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGaat                                                                                       <  2:375229/293‑1 (MQ=255)                                                                                                                                                                                                                gCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGc                                                                                  >  1:651264/1‑298 (MQ=255)                                                                                                                                                                                                                        aGTATCGAGGGTTTTTCCGCCACCGATACCGAGGATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAAt                                                                        <  1:275514/300‑1 (MQ=255)                                                                                                                                                                                                                        aGTATCGAGGGTTTTTCCGCCACCGACACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACTGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGGGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCACCCTGGATGTAGTTACCCGGTGATTGAATAATGCGGTCCATAAt                                                                        <  2:331283/300‑1 (MQ=255)                                                                                                                                                                                                                                          gCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTATAGATg                                                        <  1:709293/298‑1 (MQ=255)                                                                                                                                                                                                                                             accgctacctatactttctccACACTGCGCAGTCTCCGCTATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACTGCGCAATTTCTACTACCATTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAACTTTGTCACCCACCACTAACCAACGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAACCACATCAACGCCCTTGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGagt                                                     <  1:325421/279‑1 (MQ=255)                                                                                                                                                                                                                                             aCCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACTGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTATAGATGagtag                                                   <  2:403446/300‑1 (MQ=255)                                                                                                                                                                                                                                             aCCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTATAGATGagt                                                     <  1:669382/298‑1 (MQ=255)                                                                                                                                                                                                                                             aCCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGagtag                                                   <  1:482951/300‑1 (MQ=255)                                                                                                                                                                                                                                             aCCGATACAGAGAATTGCTCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCAACCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCATCATTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGagtag                                                   <  2:403958/300‑1 (MQ=255)                                                                                                                                                                                                                                                gATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGc                                                <  1:655487/300‑1 (MQ=255)                                                                                                                                                                                                                                                gATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGc                                                <  2:692190/300‑1 (MQ=255)                                                                                                                                                                                                                                                  tACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCa                                              <  1:728866/300‑1 (MQ=255)                                                                                                                                                                                                                                                      gagaATTTCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAAt                                           <  2:308198/299‑1 (MQ=255)                                                                                                                                                                                                                                                             gcgcCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTCGAGATGAGTAGTGCCAAATGCGGcctt                                   >  2:628475/1‑297 (MQ=255)                                                                                                                                                                                                                                                             gcgcCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCAtt                                   >  1:103334/1‑300 (MQ=255)                                                                                                                                                                                                                                                             gcgcCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCAtc                                   >  1:191812/1‑299 (MQ=255)                                                                                                                                                                                                                                                             gcgcCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTCCAGGTATTCGCCCAGACGATTAATCCCATCAGCGCACTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTCGAGATGAGTAGTGCCAAGTGCGGCAtt                                   >  1:208869/1‑300 (MQ=255)                                                                                                                                                                                                                                                             gcgcCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCAAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGTGGCTTCAGGTATTCGCCCAGACGCTTAATCACATCAGTGCACTGGATGTATTTACCCGGTGATTGAATAATGTGGt                                                                               >  2:99243/1‑256 (MQ=255)                                                                                                                                                                                                                                                             gcgcCACACTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTAATAACAGTCCAGCATCTTTAAAGCTTTTATAGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTCACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAACGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCAATAATTGCTCCTTTAGAGATGAGTAGTGCCCAATGCAGCAtt                                   >  2:99260/1‑300 (MQ=255)                                                                                                                                                                                                                                                             gcgcCACACTGCGCAGTCTCCGCGATGCCAAGCAGACGATCGATCTCATTTTGCGAACATTCCCCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTATCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTACCCAGCGTTCTGCCAGCGGTTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTAAATAATGCGGTCCATACTTGCTCCTTTCGAAATGAGCAGTGCCAAATGCGGCAt                                    >  2:102812/1‑299 (MQ=255)                                                                                                                                                                                                                                                                  acacTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGt                              >  1:261837/1‑300 (MQ=255)                                                                                                                                                                                                                                                                    acTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATTTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCATCACTAACCAGCGTTCTGCCAGCGGCTTCATGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTATAGATGAGTAGTGCCAAATGCGGCATTCCATTCg                            <  2:260856/300‑1 (MQ=255)                                                                                                                                                                                                                                                                    acTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTc                             <  1:279279/299‑1 (MQ=255)                                                                                                                                                                                                                                                                    acTGCGCAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCg                            <  1:418670/300‑1 (MQ=255)                                                                                                                                                                                                                                                                       gcgcAGTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCgtgt                         >  2:170089/1‑300 (MQ=255)                                                                                                                                                                                                                                                                            gTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGaa                    >  1:199825/1‑300 (MQ=255)                                                                                                                                                                                                                                                                            gTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCACAATTGCTCCTTTCGAGATGAGTAGTGACAAATGAGGCATTCCAGTCgt                           >  2:163268/1‑293 (MQ=255)                                                                                                                                                                                                                                                                            gTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGACCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGCATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCAAAATGCGGCATTCCAGTCGTGTTCGaa                    >  2:80915/1‑300 (MQ=255)                                                                                                                                                                                                                                                                            gTCTCCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCCTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGaa                    >  1:200624/1‑300 (MQ=255)                                                                                                                                                                                                                                                                            gTCTACGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGaa                    >  2:587954/1‑300 (MQ=255)                                                                                                                                                                                                                                                                             tctcCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAAc                   <  2:321021/300‑1 (MQ=255)                                                                                                                                                                                                                                                                              ctcCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAACACAAATTTGTCAACCACCACTAACCAGCGTTCTGCCAGCGGCNTCAGGTATTCGCACAGACGATTAATCAAATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTACTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTTGAAc                   >  2:229706/1‑299 (MQ=255)                                                                                                                                                                                                                                                                              ctcCGCGATGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACACCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACt                  >  1:589712/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                     aTGCCACGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCtat           >  2:690231/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                          aCGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGNCAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGc        <  2:570586/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                          aCGCAGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGc        <  1:310510/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                              aGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCTGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGActc  <  1:296773/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                              aGACGATCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGActc  <  1:42002/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                       TCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTC  >  NC_000913/4138524‑4139108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑