Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,637,896 A→G T401A (ACG→GCC)  creC → sensory histidine kinase CreC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,637,8960AG98.2% 193.4 / NA 57T401A (ACG→GCC) creCsensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (22/34);  total (23/34)
Fisher's exact test for biased strand distribution p-value = 4.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTT  >  NC_000913/4637604‑4638182
                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                              
tATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTaa                                                                                                                                                                                                                                                                                         >  1:393884/1‑300 (MQ=255)
  tGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAgg                                                                                                                                                                                                                                                                                       <  1:30723/300‑1 (MQ=255)
   gCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTTGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGTCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCATGCGCTTTTGAATTTACTTGCTACCGCCATCGATTCTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAgg                                                                                                                                                                                                                                                                                       <  2:251425/299‑1 (MQ=255)
                    cGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCCCCGTGCAGTTGGCAGAAAAAAACATCAATCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTAGAGCAGGCGCTGGGGAATTTACTGGATAACGCAATCGATTTTACCCCCGAGAGCGGTCGCATCACGCTCA‑ACGCCGACGTGGATCAGGAACACGT‑CGCCCCCAAGGTGCTGAATACCgttcgt                                                                                                                                                                                                                                                                      >  2:301614/1‑294 (MQ=255)
                           acGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTcc                                                                                                                                                                                                                                                              <  1:9542/300‑1 (MQ=255)
                                aGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCACTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACt                                                                                                                                                                                                                                                         >  1:275108/1‑300 (MQ=255)
                                                                  gACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTActc                                                                                                                                                                                                                       <  2:683930/300‑1 (MQ=255)
                                                                  gACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTActc                                                                                                                                                                                                                       <  1:555474/300‑1 (MQ=255)
                                                                   aCTGTTGTTGATGTTGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTActc                                                                                                                                                                                                                       <  2:5725/299‑1 (MQ=255)
                                                                       ttgTTGATGTGGCGGCATTATTTCGCCTCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGc                                                                                                                                                                                                                  <  2:649658/299‑1 (MQ=255)
                                                                       ttgTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACNTGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGc                                                                                                                                                                                                                  <  1:505852/299‑1 (MQ=255)
                                                                       ttgTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGc                                                                                                                                                                                                                  <  2:649582/299‑1 (MQ=255)
                                                                       ttgTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGc                                                                                                                                                                                                                  >  1:465914/2‑300 (MQ=255)
                                                                                     gcATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTNGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAAt                                                                                                                                                                                                       >  1:154355/1‑297 (MQ=255)
                                                                                                          gAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTTAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTCCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTgggg                                                                                                                                                                               <  2:610061/300‑1 (MQ=255)
                                                                                                          gAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTCCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTgggg                                                                                                                                                                               <  2:610118/300‑1 (MQ=255)
                                                                                                           aaGCGCGCACCGTGCAGTTGTCAGAAAAAAACATCACTCTACATTTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGTGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGt                                                                                                                                                                              <  2:161635/300‑1 (MQ=255)
                                                                                                            aGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGtt                                                                                                                                                                             >  1:547506/1‑300 (MQ=255)
                                                                                                                   aCCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCg                                                                                                                                                                         <  1:247871/297‑1 (MQ=255)
                                                                                                                      gTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTc                                                                                                                                                                   >  1:597412/1‑300 (MQ=255)
                                                                                                                      gTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCAATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTc                                                                                                                                                                   >  1:660309/1‑300 (MQ=255)
                                                                                                                           gTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGTTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGa                                                                                                                                                              <  1:164839/300‑1 (MQ=255)
                                                                                                                           gTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGa                                                                                                                                                              <  1:164830/300‑1 (MQ=255)
                                                                                                                             tGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAgg                                                                                                                                                            <  1:178449/300‑1 (MQ=255)
                                                                                                                              ggCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGt                                                                                                                                                           <  1:211153/300‑1 (MQ=255)
                                                                                                                                   aaaaaaacaGCACTCTACATTTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAACAGGCGCTGGTGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCATTCGTCAGTGAgg                                                                                                                                                            <  2:294637/294‑1 (MQ=255)
                                                                                                                                              aCTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTgggg                                                                                                                                                                               <  2:34163/264‑1 (MQ=255)
                                                                                                                                              aCTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTgggg                                                                                                                                                                               >  1:34163/1‑264 (MQ=255)
                                                                                                                                               ctctACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATTAGGAACACGTAGAC‑CTTAAGGTGCTGGATACCGGTAATGGTATTTCTGACTACGCGCTGTCACGTATCTTTNAACGCCTTTACTCTTTGCCGCGTACAACCGGGCAAAAAAGCAACGATCCGGGGTTGACGTTCTTCAGCGAGGTCGCCCgtttgttt                                                                                                                                              >  2:544205/1‑296 (MQ=255)
                                                                                                                                                           tgcccgctgAGGTTAATGTTGCTGCTGAACCGACGTTACTGGAGCAGGCGCTGGGGAATTTACTGGCTAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGGGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGc                                                                                                                              <  2:103760/292‑1 (MQ=255)
                                                                                                                                                           tgCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGc                                                                                                                              <  1:562792/299‑1 (MQ=255)
                                                                                                                                                                  tgAGGTTAATGGTGCTGCTGAACCTGCGGTACTGGAACAGGCGCTTTGGAATTTACTGGATAACACCATCGATTTTACCCCCGAGAGCGGTCGCATAGCGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATNCCTGACTACGCGCTTTCACGTATTTGTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTgcgc                                                                                                                         <  2:514600/297‑1 (MQ=255)
                                                                                                                                                                     gtgtaatgTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGt                                                                                                                    <  2:326057/293‑1 (MQ=255)
                                                                                                                                                                            gTTGCTGCTGAACCTGCGTTACTGGAGCAGGCGCTTGTGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGaa                                                                                                             <  2:377180/300‑1 (MQ=255)
                                                                                                                                                                                ctgctgAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTTCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCATTTGTTTAACGGCGAAGTCACGCTGCGCACCGTGCAGGAAGGt                                                                                                          >  2:650617/1‑299 (MQ=255)
                                                                                                                                                                                 tgctgAACCTGCGTTACTTTAGCATGCGCTGTGGAATTTACTGGAGAACGCCATCGATTCTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACTT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGt                                                                                                                    <  2:125904/288‑1 (MQ=255)
                                                                                                                                                                                                aCTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGACCACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCt                                                                                         >  1:186646/1‑300 (MQ=255)
                                                                                                                                                                                                    gAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGa                                                                                     >  1:341729/1‑300 (MQ=255)
                                                                                                                                                                                                            gctgtggaaTTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCAcc                                                                              <  2:458467/292‑1 (MQ=255)
                                                                                                                                                                                                               ggggaaTTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGTTGGATCCTGGTAGTGGTATTCATGACTACGCGCTGTAAAGTATTTTTGAACGCTTTTCCTCTTTGCCGCGTGCAAATGGGCAAAACAGCAGCGATCTGGGGTTGGCATTCGNCCGTGAGGTCGCCCTTTTGTTTACCGTCGAAGTCCCGCTGCGCACCACGCAGGCAGGCAGCGTGCTAGCCTCGCTTCGACTTCACCGTCa                                                                          >  2:543460/5‑300 (MQ=255)
                                                                                                                                                                                                                  gaaTTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACtt                                                                       >  1:671724/2‑300 (MQ=255)
                                                                                                                                                                                                                       tACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGNCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACAt                                                                  <  1:507415/300‑1 (MQ=255)
                                                                                                                                                                                                                       tACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACAt                                                                  <  2:654404/300‑1 (MQ=255)
                                                                                                                                                                                                                                           tttACCCCCGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCNGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCcaca                                              <  1:131496/300‑1 (MQ=255)
                                                                                                                                                                                                                                                 cccGAGAGCGGTCGCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTAACTTCACATAGCTTCAAATTCTTCCCACATAgcct                                        >  2:259130/1‑297 (MQ=255)
                                                                                                                                                                                                                                                          gtccgCATAACGCTAACNCGCCGAAGTGGATCAGGANCACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCNCATAGCCTTCGTATcc                                <  1:575543/296‑1 (MQ=255)
                                                                                                                                                                                                                                                          ggtcgCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATcc                                <  1:336974/295‑1 (MQ=255)
                                                                                                                                                                                                                                                              gCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTNGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCctgct                            >  1:135978/1‑299 (MQ=255)
                                                                                                                                                                                                                                                              gCATAACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCctgctg                           <  1:77832/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                           gcgcCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGCCTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCaaa                 >  2:310658/1‑298 (MQ=255)
                                                                                                                                                                                                                                                                           gcgcCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTAAGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCACGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTAAAATTCTTCCAACATAGCCTTCGTATCCTGCTGCCATTGCaaa                 >  2:68358/1‑298 (MQ=255)
                                                                                                                                                                                                                                                                             gcCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCATTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAgg               <  1:24711/298‑1 (MQ=255)
                                                                                                                                                                                                                                                                                  aGTGGATCAGGAACACGT‑CGCCCTTAATGTGCGGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGAc        <  2:467813/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                  aGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCGCGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAACAGCAGTGGTATGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCAACGTCACTTCACATAGCTTAAAATTCTTCCCACATAGCCTTCGTATCCTGCTGTTATTGCAAAGGaggaga         >  2:184251/1‑299 (MQ=255)
                                                                                                                                                                                                                                                                                     ggATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTAt     >  2:425888/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                      gATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTAt     >  1:666540/1‑299 (MQ=255)
                                                                                                                                                                                                                                                                                         cAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGtt  >  2:158193/1‑299 (MQ=255)
                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                              
TATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAA‑GCGCCGAAGTGGATCAGGAACACGT‑CACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTT  >  NC_000913/4637604‑4638182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑