Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,637,857	T→C	C388R (TGC→CGC)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,637,857	0	T	C	100.0%	188.4 / NA	55	C388R (TGC→CGC)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/35);  total (20/35)

TCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCT  >  NC_000913/4637568‑4638144                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                 tCGTTTTACCGACAACATTCTTACGCAAAATGCGCGTATGCATGCATTGGTATAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGNCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTTGGGAATTTACTGGATACCGCCATCGAGTTGACCACCGAGAGCGGTCGCATAACGc                                                                                                                                                                                                                                                                                         <  2:219072/299‑1 (MQ=255) tCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATTTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGc                                                                                                                                                                                                                                                                                         <  1:193126/299‑1 (MQ=255) tCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGc                                                                                                                                                                                                                                                                                         <  1:249296/299‑1 (MQ=255)   ggtttACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCATGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCTGCGTTACTGGAGCAGGCGCTTTGGAATTTACTCGATCGTGCTAGCGATTTTACCCCCGAGAGCGGTCGCATAACGc                                                                                                                                                                                                                                                                                         <  2:563938/295‑1 (MQ=255)   gTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTaa                                                                                                                                                                                                                                                                                      <  2:574649/300‑1 (MQ=255)     tttACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAgc                                                                                                                                                                                                                                                                                    <  1:638922/300‑1 (MQ=255)                                     tATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTaa                                                                                                                                                                                                                                                   >  1:393884/1‑300 (MQ=255)                                     tATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTAAATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑cgaccttaa                                                                                                                                                                                                                                                   >  1:393302/1‑292 (MQ=255)                                       tGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAgg                                                                                                                                                                                                                                                 <  1:30723/300‑1 (MQ=255)                                        gCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTTGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGTCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCATGCGCTTTTGAATTTACTTGCTACCGCCATCGATTCTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAgg                                                                                                                                                                                                                                                 <  2:251425/299‑1 (MQ=255)                                           ggCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTAAATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGCTTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑cgaccgtaaggtgct                                                                                                                                                                                                                                             >  2:569724/1‑286 (MQ=255)                                                         cGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCCCCGTGCAGTTGGCAGAAAAAAACATCAATCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTAGAGCAGGCGCTGGGGAATTTACTGGATAACGCAATCGATTTTACCCCCGAGAGCGGTCGCATCACGCTCAACGCCGACGTGGATCAGGAACACGT‑CGCCCCCAAGGTGCTGAATACCgttcgt                                                                                                                                                                                                                                >  2:301614/1‑294 (MQ=255)                                                                acGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTcc                                                                                                                                                                                                                        <  1:9542/300‑1 (MQ=255)                                                                 cGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAACAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATCAc                                                                                                                                                                                                                                                                                           >  2:575543/1‑233 (MQ=255)                                                                     aGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGCACTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACt                                                                                                                                                                                                                   >  1:275108/1‑300 (MQ=255)                                                                                                       gACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTActc                                                                                                                                                                                 <  1:555474/300‑1 (MQ=255)                                                                                                       gACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTActc                                                                                                                                                                                 <  2:683930/300‑1 (MQ=255)                                                                                                        aCTGTTGTTGATGTTGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTActc                                                                                                                                                                                 <  2:5725/299‑1 (MQ=255)                                                                                                            ttgTTGATGTGGCGGCATTATTTCGCCTCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGc                                                                                                                                                                            <  2:649658/299‑1 (MQ=255)                                                                                                            ttgTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACNTGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGc                                                                                                                                                                            <  1:505852/299‑1 (MQ=255)                                                                                                            ttgTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGc                                                                                                                                                                            <  2:649582/299‑1 (MQ=255)                                                                                                            ttgTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGc                                                                                                                                                                            >  1:465914/2‑300 (MQ=255)                                                                                                                          gcATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTNGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAAt                                                                                                                                                                 >  1:154355/1‑297 (MQ=255)                                                                                                                                               gAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTTAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTCCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTgggg                                                                                                                                         <  2:610061/300‑1 (MQ=255)                                                                                                                                               gAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTCCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTgggg                                                                                                                                         <  2:610118/300‑1 (MQ=255)                                                                                                                                                aaGCGCGCACCGTGCAGTTGTCAGAAAAAAACATCACTCTACATTTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGTGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGt                                                                                                                                        <  2:161635/300‑1 (MQ=255)                                                                                                                                                 aGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGtt                                                                                                                                       >  1:547506/1‑300 (MQ=255)                                                                                                                                                        aCCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCg                                                                                                                                   <  1:247871/297‑1 (MQ=255)                                                                                                                                                           gTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTc                                                                                                                             >  1:597412/1‑300 (MQ=255)                                                                                                                                                           gTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCAATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTc                                                                                                                             >  1:660309/1‑300 (MQ=255)                                                                                                                                                                gTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGTTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGa                                                                                                                        <  1:164839/300‑1 (MQ=255)                                                                                                                                                                gTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGa                                                                                                                        <  1:164830/300‑1 (MQ=255)                                                                                                                                                                  tGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAgg                                                                                                                      <  1:178449/300‑1 (MQ=255)                                                                                                                                                                   ggCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGt                                                                                                                     <  1:211153/300‑1 (MQ=255)                                                                                                                                                                        aaaaaaacaGCACTCTACATTTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAACAGGCGCTGGTGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCATTCGTCAGTGAgg                                                                                                                      <  2:294637/294‑1 (MQ=255)                                                                                                                                                                                   aCTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTgggg                                                                                                                                         <  2:34163/264‑1 (MQ=255)                                                                                                                                                                                   aCTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTgggg                                                                                                                                         >  1:34163/1‑264 (MQ=255)                                                                                                                                                                                    ctctACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATTAGGAACACGTAGAC‑CTTAAGGTGCTGGATACCGGTAATGGTATTTCTGACTACGCGCTGTCACGTATCTTTNAACGCCTTTACTCTTTGCCGCGTACAACCGGGCAAAAAAGCAACGATCCGGGGTTGACGTTCTTCAGCGAGGTCGCCCgtttgttt                                                                                                        >  2:544205/1‑296 (MQ=255)                                                                                                                                                                                                tgcccgctgAGGTTAATGTTGCTGCTGAACCGACGTTACTGGAGCAGGCGCTGGGGAATTTACTGGCTAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGGGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGc                                                                                        <  2:103760/292‑1 (MQ=255)                                                                                                                                                                                                tgCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGc                                                                                        <  1:562792/299‑1 (MQ=255)                                                                                                                                                                                                       tgAGGTTAATGGTGCTGCTGAACCTGCGGTACTGGAACAGGCGCTTTGGAATTTACTGGATAACACCATCGATTTTACCCCCGAGAGCGGTCGCATAGCGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATNCCTGACTACGCGCTTTCACGTATTTGTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTgcgc                                                                                   <  2:514600/297‑1 (MQ=255)                                                                                                                                                                                                          gtgtaatgTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGt                                                                              <  2:326057/293‑1 (MQ=255)                                                                                                                                                                                                          ggTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCAt                                                                                                                                                                                                                                                                                              >  2:482799/1‑93 (MQ=255)                                                                                                                                                                                                                 gTTGCTGCTGAACCTGCGTTACTGGAGCAGGCGCTTGTGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGaa                                                                       <  2:377180/300‑1 (MQ=255)                                                                                                                                                                                                                     ctgctgAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTTCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCATTTGTTTAACGGCGAAGTCACGCTGCGCACCGTGCAGGAAGGt                                                                    >  2:650617/1‑299 (MQ=255)                                                                                                                                                                                                                      tgctgAACCTGCGTTACTTTAGCATGCGCTGTGGAATTTACTGGAGAACGCCATCGATTCTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACTT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGt                                                                              <  2:125904/288‑1 (MQ=255)                                                                                                                                                                                                                                     aCTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGACCACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCt                                                   >  1:186646/1‑300 (MQ=255)                                                                                                                                                                                                                                         gAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGa                                               >  1:341729/1‑300 (MQ=255)                                                                                                                                                                                                                                                 gctgtggaaTTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCAcc                                        <  2:458467/292‑1 (MQ=255)                                                                                                                                                                                                                                                    ggggaaTTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGTTGGATCCTGGTAGTGGTATTCATGACTACGCGCTGTAAAGTATTTTTGAACGCTTTTCCTCTTTGCCGCGTGCAAATGGGCAAAACAGCAGCGATCTGGGGTTGGCATTCGNCCGTGAGGTCGCCCTTTTGTTTACCGTCGAAGTCCCGCTGCGCACCACGCAGGCAGGCAGCGTGCTAGCCTCGCTTCGACTTCACCGTCa                                    >  2:543460/5‑300 (MQ=255)                                                                                                                                                                                                                                                       gaaTTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACtt                                 >  1:671724/2‑300 (MQ=255)                                                                                                                                                                                                                                                            tACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGNCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACAt                            <  1:507415/300‑1 (MQ=255)                                                                                                                                                                                                                                                            tACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACAt                            <  2:654404/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                tttACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCNGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCcaca        <  1:131496/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                      cccGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTAACTTCACATAGCTTCAAATTCTTCCCACATAgcct  >  2:259130/1‑297 (MQ=255)                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                 TCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACACGT‑CACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCT  >  NC_000913/4637568‑4638144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑