Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,630,304	A→G	Y73Y (TAT→TAC)	ettA ←	energy‑dependent translational throttle protein EttA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,630,304	0	A	G	98.9%	302.3 / NA	90	Y73Y (TAT→TAC)	ettA	energy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (57/32);  minor base T (0/1);  total (57/33)
Fisher's exact test for biased strand distribution p-value = 3.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCG‑TCGTGAGCCTGAATGATC‑TCTTCCA‑GACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATAATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCC  >  NC_000913/4630013‑4630598                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                        gcgACGACGCTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCGAGCTGTACGTTCAGGTTATGACCG‑TCGTGAGCCTGAATGATC‑TCTTCCA‑GACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCaa                                                                                                                                                                                                                                                                                                  <  1:675649/299‑1 (MQ=255)         gctCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCG‑TCGTGAGCCTGAATGATC‑TCTTCCA‑GACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGt                                                                                                                                                                                                                                                                                         >  1:83682/3‑300 (MQ=255)            caccacCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCG‑TCGTGAGCCTGAATGATC‑TCTTCCA‑GACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTAATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTAAATGGACTCACGCCCGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTc                                                                                                                                                                                                                                                                                        >  2:201972/1‑298 (MQ=255)            caccacCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCAGCACGCTCCAGCTGTACGTTCAGGTTATGACCG‑TCGTGAGCCTGAATGATC‑TCTTACA‑GACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCt                                                                                                                                                                                                                                                                                       >  2:201863/1‑299 (MQ=255)                 ccGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCG‑TCGTGAGCCTGAATGATC‑TCTTCCA‑GACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGc                                                                                                                                                                                                                                                                                 >  2:111045/1‑300 (MQ=255)                 ccGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCG‑TCGTGAGCCTGAATGATC‑TCTTCCA‑GACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTATTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGc                                                                                                                                                                                                                                                                                 >  2:247325/1‑300 (MQ=255)                 ccGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCG‑TCGTGAGCCTGAATGATC‑TCTTCCA‑GACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTAAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGc                                                                                                                                                                                                                                                                                 >  2:151057/1‑300 (MQ=255)                         gTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCG‑TCGTGAGCCTGAATGATC‑TCTTCCA‑GACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACgcgc                                                                                                                                                                                                                                                                         <  1:690841/300‑1 (MQ=255)                                 ttttCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCG‑TCGTGAGCCTGAATGATC‑TCTTCCA‑GACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCtt                                                                                                                                                                                                                                                                 <  1:549661/300‑1 (MQ=255)                                    tCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCTGCACGCTCCAGCTGTACGTTCAGGTTATGACCG‑TCGTGAGCCTGAATGATC‑TCTTCCA‑GACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGa                                                                                                                                                                                                                                                              <  1:255234/300‑1 (MQ=255)                                      gcgTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCGAGCTGTACGTTCAGGTTATGACCG‑TCGTGAGCCTGAATGATC‑TCTTCCA‑GACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGAt                                                                                                                                                                                                                                                             <  1:202535/299‑1 (MQ=255)                                      gcgTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCG‑TCGTGAGCCTGAATGATC‑TCTTCCA‑GACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGa                                                                                                                                                                                                                                                              <  1:254465/298‑1 (MQ=255)                                         tCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCG‑TCGTGAGCCTGAATGATC‑TCTTCCA‑GACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCt                                                                                                                                                                                                                                                         >  1:114183/1‑300 (MQ=255)                                              gTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCG‑TCGTGAGCCTGAATGATC‑TCTTCCA‑GACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATc                                                                                                                                                                                                                                                    >  1:455237/1‑300 (MQ=255)                                                          tAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCG‑TCGTGAGCCTGAATGATC‑TCTTCCA‑GACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCAt                                                                                                                                                                                                                                        >  1:10170/1‑300 (MQ=255)                                                            gcgcATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCG‑TCGTGAGCCTGAATGATC‑TCTTCCA‑GACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATaa                                                                                                                                                                                                                                      <  1:250186/300‑1 (MQ=255)                                                                    gccgcACGCTCCAGCTGTACGTGCAGGTTATGACCG‑TCGTGAGCCTGAATGATC‑TCTTCCA‑GACGGCCTTGTTCAGCGGCCAGCTTTGCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCgcag‑c                                                                                                                                                                                                                             <  2:366674/300‑1 (MQ=255)                                                                       gcACGCTCCAGCTGTACGTTCAGGTTATGATCG‑TCGTGAGCCTGAATGATC‑TCTTCCA‑GACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGcag‑cag                                                                                                                                                                                                                           <  1:225970/299‑1 (MQ=255)                                                                       gcACGCTCCAGCTGTACGTTCAGGTTATGACCG‑TCGTGAGCCTGAATGATC‑TCTTCCA‑GACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGcag‑cag                                                                                                                                                                                                                           <  1:225320/299‑1 (MQ=255)                                                                         aCGCTCTAGCTGTACGTTCAGGTTATGACCG‑TCGTGAGCCTGAATGATC‑TCTTCCA‑GACGGCCTTGTTCAGCTGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTGCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAAGGCCCGCCATAATGCGcag‑cag                                                                                                                                                                                                                           <  1:225353/297‑1 (MQ=255)                                                                          cGCTCCAGCTGTACGTTCAGGTTATGACCG‑TCGTGAGCCTGAATGATC‑TCTTCCA‑GACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCCG‑CAGGGta                                                                                                                                                                                                                       >  2:164766/1‑299 (MQ=255)                                                                          cGCTCCAGCTGTACGTTCAGGTTATGACCG‑TCGTGAGCCTGAATGATC‑TCTTCCA‑GACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGta                                                                                                                                                                                                                       >  1:126250/1‑299 (MQ=255)                                                                            cTCCAGCTGTACGTTCAGGTTATGACCG‑TCGTGAGCCTGAATGATC‑TCTTCCA‑GACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCTGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGtaga                                                                                                                                                                                                                     >  2:345325/1‑297 (MQ=255)                                                                            cTCCAGCTGTACGTTCAGGTTATGACCG‑TCGTGAGCCTGAATGATC‑TCTTCCA‑GACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTAAGGGCGTTAACCACTTCAGAAACCGCTTCTTAANTGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCCGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTAACCTTCGATGTCTTTCTCAATGCCCGCCATAATGCGCAG‑CAGGGta                                                                                                                                                                                                                       >  2:229245/1‑297 (MQ=255)                                                                              ccAGCTGTACGTTCAGGTTATGACCG‑TCGTGAGCCTGAATGATC‑TCTTCCA‑GACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACt                                                                                                                                                                                                                   >  2:155705/1‑300 (MQ=255)                                                                                aGCTGTACGTTCAGGTTATGACCG‑TCGTGAGCCTGAATGATC‑TCTTCCA‑GACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACt                                                                                                                                                                                                                   <  1:30728/298‑1 (MQ=255)                                                                                    gTACGTTCAGGTTATGACCG‑TCGTGAGCCTGAATGATC‑TCTTCCA‑GACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTCTCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTAcc                                                                                                                                                                                                               >  2:38181/1‑298 (MQ=255)                                                                                      aCGTTCAGGTTATGACCG‑TCGTGAGCCTGAATGATC‑TCTTCCA‑GACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCcgc                                                                                                                                                                                                            >  1:9317/1‑299 (MQ=255)                                                                                       gttcgaggttATGACCGNTCGTGAGCCTGAATGATCNTCTTCCACGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGNCAGGGTAGACTTAccc                                                                                                                                                                                                              <  1:578584/292‑1 (MQ=255)                                                                                        gTTCAGGTTATGACCG‑TCGTGAGCCTGAATGATC‑TCTTCCA‑GACTGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCgcgc                                                                                                                                                                                                          <  2:285494/299‑1 (MQ=255)                                                                                            aggttATGACCG‑TCGTGAGCCTGAATGATC‑TCTTCCA‑GACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACNGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGNAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTc                                                                                                                                                                                                     <  2:528763/297‑1 (MQ=255)                                                                                             ggttATGACCG‑TCGTGAGCCTGAATGATC‑TCTTCCA‑GACGGCCTTGTTCAGCGGCCGGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTAGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGAGTAGACTTACCCGCGCCGTTCa                                                                                                                                                                                                    >  2:75882/3‑300 (MQ=255)                                                                                             ggttATGACCG‑TCGTGAGCCTGAATGATC‑TCTTCCA‑GACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCCCGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTAACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCa                                                                                                                                                                                                    >  1:363144/3‑300 (MQ=255)                                                                                             ggttATGACCG‑TCGTGAGCCTGAATGATC‑TCTTCCA‑GACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCa                                                                                                                                                                                                    >  1:364727/3‑300 (MQ=255)                                                                                             ggttATGACCG‑TCGTGAGCCTGAATGATC‑TCTTCCA‑GACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCCGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCa                                                                                                                                                                                                    >  1:252989/3‑300 (MQ=255)                                                                                              gttATGACCG‑TCGTGAGCCTGAATGATC‑TCTTCCA‑GACGGCCTTGTTCAGCGGCCAGCTTGGCAAAATCGGCATCCGGGTCTGCGTACAGCGCATATACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACNGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCTGNGGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATTTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCTGCGCCGTTc                                                                                                                                                                                                     <  2:528892/297‑1 (MQ=255)                                                                                                 aTGACCG‑TCGTGAGCCTGAATGATC‑TCTTCCA‑GACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGAcc                                                                                                                                                                                                <  1:423388/300‑1 (MQ=255)                                                                                                 aTGACCG‑TCGTGAGCCTGAATGATC‑TCTTCCA‑GACGGCCTTGGTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGAcc                                                                                                                                                                                                <  1:451168/300‑1 (MQ=255)                                                                                                                ccTGAATGATC‑TCTTCCA‑GACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTAcc                                                                                                                                                                                                               <  1:691111/271‑1 (MQ=255)                                                                                                                ccTGAATGATC‑TCTTCCA‑GACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTAcc                                                                                                                                                                                                               >  2:691111/1‑271 (MQ=255)                                                                                                                             ctTCCA‑GACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGga                                                                                                                                                                      >  2:4609/1‑300 (MQ=255)                                                                                                                             ctTCCA‑GACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGga                                                                                                                                                                      >  1:604563/1‑300 (MQ=255)                                                                                                                             ctTCCA‑GACGGCCTTGTTCAGCGGCCAGATTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTAAATGGACTCACGCACGGTGTGTTCCGGGTTTAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATATCTGGCTGCGGACGCGCTTCAACTTCGATGTCTTTATCAATGCCCGCCATCATGCGCAG‑CAGGGTAGACTTACCTGCGCAGTTCAGACCCAGGACAACAAGTTTTGCACCAAGga                                                                                                                                                                      >  2:4617/1‑300 (MQ=255)                                                                                                                                        gCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCa                                                                                                                                                             >  2:576506/1‑299 (MQ=255)                                                                                                                                        gCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTATTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTNATCAATGCCCGCAATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCAAGGGAAGAAACtaa                                                                                                                                                             >  2:538855/1‑297 (MQ=255)                                                                                                                                        gCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCNTCTTCAATGGACTCACGCACGGTGTGTTCCGGNTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCa                                                                                                                                                             >  2:528800/1‑299 (MQ=255)                                                                                                                                           ttGTTCAGCGGCCAGCTGGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCagaga                                                                                                                                                         <  2:70438/300‑1 (MQ=255)                                                                                                                                                         gCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCaaaa                                                                                                                                           >  1:278676/1‑300 (MQ=255)                                                                                                                                                                             ggATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGaac                                                                                                                        <  2:620332/299‑1 (MQ=255)                                                                                                                                                                               atctgCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCTGTTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACATTTCGGCGGaac                                                                                                                        <  2:620339/293‑1 (MQ=255)                                                                                                                                                                                  ggCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACttt                                                                                                                  >  1:445732/1‑300 (MQ=255)                                                                                                                                                                                   gCGTCCAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCAAGGGAAGAAACTCAGAGAGATGTTTTTCAACATCTGACGTTTCGGCGGAACAACttt                                                                                                                  >  1:272721/1‑299 (MQ=255)                                                                                                                                                                                   gCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCNAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATCTGACGTTTCAGCGGAACAACttt                                                                                                                  >  1:494415/1‑299 (MQ=255)                                                                                                                                                                                   gCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACttt                                                                                                                  >  1:85775/1‑299 (MQ=255)                                                                                                                                                                                   gCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCAAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTAGGCGGAACAACttt                                                                                                                  >  1:426295/1‑299 (MQ=255)                                                                                                                                                                                   gCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTAACCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAAAttt                                                                                                                  >  2:78468/1‑299 (MQ=255)                                                                                                                                                                                   gCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTCCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACttt                                                                                                                  >  1:426303/1‑299 (MQ=255)                                                                                                                                                                                        cAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCACGCAATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACAACAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTAGGCGGAACAACTTTGCCGac                                                                                                            >  1:90119/1‑300 (MQ=255)                                                                                                                                                                                                 cacTTCGTCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCAAAAGGGAAGAAACTCAGCGAGATGTTTTTCAACATATGACGTTTCGGCGGAACAACTTTGCCGACACggtgtat                                                                                                   >  2:179870/1‑294 (MQ=255)                                                                                                                                                                                                 cacTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCAt                                                                                                   >  2:179861/1‑300 (MQ=255)                                                                                                                                                                                                 cacTTAATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTAAATGGACCCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTAACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCACCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAAAGAGACGTTTTTCAAAATATGACGTTTCGACGGAACAACTTTGCCAACACGa                                                                                                        >  2:367299/1‑295 (MQ=255)                                                                                                                                                                                                    ttCATCCAGGCGTTTCAGTGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGt                                                                                                <  2:599876/300‑1 (MQ=255)                                                                                                                                                                                                     tCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCAAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGta                                                                                               >  1:68615/1‑300 (MQ=255)                                                                                                                                                                                                           aGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACg                                                                                         <  2:151435/300‑1 (MQ=255)                                                                                                                                                                                                           aGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACg                                                                                         <  1:247325/300‑1 (MQ=255)                                                                                                                                                                                                            ggcctttCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCTGGTTCAGCTGCGGTTCCTGCGGCAGTTAACCAATCTTGGTGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGNAG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGTGAAGAAACTCAGAGAGATGTTTTTCAAAATACGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACg                                                                                         <  2:509907/295‑1 (MQ=255)                                                                                                                                                                                                                             aCCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGAc                                                                       <  1:89997/300‑1 (MQ=255)                                                                                                                                                                                                                                            gCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGttt                                                        <  1:111045/300‑1 (MQ=255)                                                                                                                                                                                                                                                 ttcAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGt                                                   <  2:278676/300‑1 (MQ=255)                                                                                                                                                                                                                                                          cTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATNCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCNGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGtgatgatga                                           >  2:520323/1‑299 (MQ=255)                                                                                                                                                                                                                                                          cTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCNGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCAAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGtgatgatga                                           >  2:508781/1‑299 (MQ=255)                                                                                                                                                                                                                                                          cTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGtgatgatga                                           >  1:97736/1‑299 (MQ=255)                                                                                                                                                                                                                                                                    gtgtgtTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCaaa                                  >  2:422867/1‑298 (MQ=255)                                                                                                                                                                                                                                                                    gtgtgtTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCaaa                                  >  1:418588/1‑298 (MQ=255)                                                                                                                                                                                                                                                                    gtgtgtTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTCCGCCTCTATGTTTCTCGTGATGATGAGTTTTCaaa                                  >  1:418580/1‑298 (MQ=255)                                                                                                                                                                                                                                                                        gtTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGaa                            >  2:417596/1‑300 (MQ=255)                                                                                                                                                                                                                                                                        gtTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGaa                            >  1:532146/1‑300 (MQ=255)                                                                                                                                                                                                                                                                        gtTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCAACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGaa                            >  2:138261/1‑300 (MQ=255)                                                                                                                                                                                                                                                                        gtTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCAAGGGAAGAAACTCAGAGAGATGTTTTTAAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGaa                            >  2:207134/1‑300 (MQ=255)                                                                                                                                                                                                                                                                             gggTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGAcc                                                                                                                                                                                                <  2:221231/131‑1 (MQ=255)                                                                                                                                                                                                                                                                             gggTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGAcc                                                                                                                                                                                                >  1:221231/1‑131 (MQ=255)                                                                                                                                                                                                                                                                               gTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGc                     >  2:443087/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                  cAGCTGCGGTTCCTGCGGCAGGTAGCCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTAGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTATATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTTTAGCCtt                  >  2:649194/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                  cAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCtt                  >  1:551486/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                            tCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAGCTTTGCCGAAACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGcc        >  2:453020/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                            tCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTATATGTTTATCGTGATGATGAGTTTTAAAAGGCGAAGTGTAGCCTTTTTCCCCGcc        >  2:452660/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                            tCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCAACTTCGCTGTCTTTACCCATGCCCACCATCATCCGCAA‑CAGGGTAGCCTTCCCCGCTCCATTCAGAACCAGTCCACCAATTCTTACACCAAGGAAGAACCTAAGCAAGATGCTTTTCAAACTATGCCGTTCAGGCGGAACAACTTTGCAGGCGCGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGTCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGcc        >  2:452652/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                  ggCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCNG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATcc  <  1:151057/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                  ggCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATcc  <  2:364727/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                   gCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CATGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCATGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATcc  <  2:8819/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                        GCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCG‑TCGTGAGCCTGAATGATC‑TCTTCCA‑GACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAG‑CAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATAATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCC  >  NC_000913/4630013‑4630598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑