Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,626,521	T→C	L204L (TTG→CTG)	radA →	DNA recombination protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,626,521	0	T	C	100.0%	203.9 / NA	58	L204L (TTG→CTG)	radA	DNA recombination protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/24);  total (34/24)

GGGGAAATCCACGCTGCTACTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGT  >  NC_000913/4626229‑4626802                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                          ggggAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCATGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGacgc                                                                                                                                                                                                                                                                                     >  2:10037/1‑297 (MQ=255)      aaTCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTc                                                                                                                                                                                                                                                                                >  1:259795/1‑299 (MQ=255)        tCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGTTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGcc                                                                                                                                                                                                                                                                             <  1:474449/300‑1 (MQ=255)                gttgcTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGANACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAAcgc                                                                                                                                                                                                                                                                      >  2:497190/5‑299 (MQ=255)                    cTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGgt                                                                                                                                                                                                                                                                 >  1:116338/1‑300 (MQ=255)                    cTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGgt                                                                                                                                                                                                                                                                 >  2:416323/1‑300 (MQ=255)                    cTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACAGGCGAAGAGTCGCTGCAAAAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGgt                                                                                                                                                                                                                                                                 >  2:4170/1‑300 (MQ=255)                            gCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGa                                                                                                                                                                                                                                                          <  1:416323/299‑1 (MQ=255)                                     aCTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTTTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCTAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGTTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCAtggt                                                                                                                                                                                                                                                <  2:353502/300‑1 (MQ=255)                                             agcagATGAAAACGCTGTATGTCACCGTCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCAtggttggacac                                                                                                                                                                                                                                         <  2:630497/299‑8 (MQ=255)                                             agcagATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCTAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGTTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGTAGGTGCGTGAAACTGCGGCTTATCTGACGCACGGCGCCAAAACGCGCGGTGTGGCGATTGTCAtggttggacac                                                                                                                                                                                                                                         <  1:37597/299‑8 (MQ=255)                                                             gTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATggctc                                                                                                                                                                                                                        >  1:121826/1‑297 (MQ=255)                                                             gTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGACAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATggctc                                                                                                                                                                                                                        >  2:583676/1‑297 (MQ=255)                                                                              agTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGTTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAg                                                                                                                                                                                                       <  1:478652/300‑1 (MQ=255)                                                                              agTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAg                                                                                                                                                                                                       <  1:476458/300‑1 (MQ=255)                                                                                  gCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAAGACGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAGAGATGGCTCGCTGGCGTGCCCTAAAGTGc                                                                                                                                                                                                    <  1:655322/299‑1 (MQ=255)                                                                                   cTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCt                                                                                                                                                                                                   >  1:1052/1‑299 (MQ=255)                                                                                       aaCAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAac                                                                                                                                                                                              <  1:597595/300‑1 (MQ=255)                                                                                        aCAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAaca                                                                                                                                                                                             >  2:423364/1‑300 (MQ=255)                                                                                          aGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCNACCGAAGCTGATGGTAATTGACTCGATCCAGGNGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACt                                                                                                                                                                                           >  2:520935/1‑300 (MQ=255)                                                                                          aGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAacac                                                                                                                                                                                            <  1:68439/299‑1 (MQ=255)                                                                                          aGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACt                                                                                                                                                                                           >  1:610594/1‑300 (MQ=255)                                                                                                         cTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGCAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGTGGGCCCTAAAGTGCTGGAAAACTGTATCGACTGtt                                                                                                                                                                               >  2:182561/1‑297 (MQ=255)                                                                                                            aTCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGNTGCGTGAAACGGCGGCTTATCTGACGCGCTTCNCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGc                                                                                                                                                                         <  2:531259/300‑1 (MQ=255)                                                                                                               gCCTTGGCCTGCCGCCTGACAATCTCAATACGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCAGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTAt                                                                                                                                                                                       >  2:490012/1‑283 (MQ=255)                                                                                                                  ttGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTg                                                                                                                                                                    <  2:674923/299‑1 (MQ=255)                                                                                                                    ggCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATTTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGAACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACCCTGTATCGACTGTTCGGTGCTTTTGGAt                                                                                                                                                                 >  2:114082/1‑300 (MQ=255)                                                                                                                             aCTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGcc                                                                                                                                                        <  1:642198/300‑1 (MQ=255)                                                                                                                                     tctcAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTccc                                                                                                                                                 >  1:570800/1‑299 (MQ=255)                                                                                                                                     tctcAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTCTTGGCTGGCGATGTCGACTccc                                                                                                                                                 >  2:103248/1‑299 (MQ=255)                                                                                                                                                     gAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCAc                                                                                                                                       >  1:568469/1‑293 (MQ=255)                                                                                                                                                     gAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCAc                                                                                                                                       <  2:568469/293‑1 (MQ=255)                                                                                                                                                       aaCCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCa                                                                                                                               >  2:397738/1‑299 (MQ=255)                                                                                                                                                            gCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATa                                                                                                                         >  2:314855/1‑300 (MQ=255)                                                                                                                                                              aTCGATCAGATCTGCCTAATTGCCTAATAAGAGCAACCGAAACTTATGGTAATCTACTCAATCAAGGTGATGCATAT‑GACGGATGGACAGGCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGGCACGTAACCAAAGATGGCTCGTTGGCGGGCCCTGAGGTGCTTGAACACTGTATCGACTGTTCGGTGCTTTTGGa                                                                                                                                                                  <  2:98633/257‑1 (MQ=255)                                                                                                                                                               tCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATaaaa                                                                                                                      <  2:570383/300‑1 (MQ=255)                                                                                                                                                                 gagcAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGTTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCCTAAAAAc                                                                                                                    >  2:245577/4‑300 (MQ=255)                                                                                                                                                                      gctCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTAGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAAccattt                                                                                                               >  2:140041/3‑296 (MQ=255)                                                                                                                                                                      gATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTcccat                                                                                                                                               >  2:382642/1‑266 (MQ=255)                                                                                                                                                                      gATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAACCCGCt                                                                                                                >  2:382658/1‑299 (MQ=255)                                                                                                                                                                      gATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCtt                                                                                                               >  1:190510/1‑300 (MQ=255)                                                                                                                                                                                         gaagaGCAAACGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCACGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCt                                                                                             >  2:278532/1‑299 (MQ=255)                                                                                                                                                                                                      aGCTGATGGTAATTGACTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGa                                                                               >  2:123977/1‑300 (MQ=255)                                                                                                                                                                                                               tAATTGACTCGATCCAGTTGATGCATAT‑GGCGGATGTACAGTCATCGCCTTGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAAc                                                                      <  2:238258/300‑1 (MQ=255)                                                                                                                                                                                                                     aCTCGATCCAGGTGATGCATAT‑GTCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGc                                                                <  1:56777/300‑1 (MQ=255)                                                                                                                                                                                                                     aCTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGc                                                                <  2:97237/300‑1 (MQ=255)                                                                                                                                                                                                                     aCTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGc                                                                <  1:55928/300‑1 (MQ=255)                                                                                                                                                                                                                          tccacgttGATGCATATNGGCGGATGTACAGTCATCNCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGNCCGAACAGGGGCTGCg                                                            <  1:575826/293‑1 (MQ=255)                                                                                                                                                                                                                          aTCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGTGGCTGCGt                                                           >  1:689063/1‑300 (MQ=255)                                                                                                                                                                                                                          aTCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTgagt                                                           >  2:450869/1‑297 (MQ=255)                                                                                                                                                                                                                          aTCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGt                                                           >  1:541076/1‑300 (MQ=255)                                                                                                                                                                                                                          aTCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTAGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAANATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGt                                                           >  2:506036/1‑300 (MQ=255)                                                                                                                                                                                                                                        tat‑GGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAAccc                                             <  1:360475/300‑1 (MQ=255)                                                                                                                                                                                                                                                gATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAAAACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCa                                      >  2:370363/1‑300 (MQ=255)                                                                                                                                                                                                                                                gATGTACAGTCATCGCCTGACAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCACGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTACAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTAGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCa                                      >  2:388939/1‑300 (MQ=255)                                                                                                                                                                                                                                                                           gcgcAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGt            >  1:121942/1‑299 (MQ=255)                                                                                                                                                                                                                                                                               aGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGAcc        <  1:532588/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                     gTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCggc  <  1:448478/299‑2 (MQ=255)                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                          GGGGAAATCCACGCTGCTACTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATAT‑GGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGT  >  NC_000913/4626229‑4626802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑