Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,822	T→C	D363D (GAT→GAC)	thrC →	threonine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,822	0	T	C	98.7%	240.4 / NA	79	D363D (GAT→GAC)	thrC	threonine synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (62/15);  minor base A (1/0);  total (63/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACTTCGGAGCCGCACGCTGCCGTAGCTTATCGTGCGCTGCGTGATCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGAT‑‑G‑‑AATCAT‑‑CAGTAAAATCTATTCAT‑‑TATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGA  >  NC_000913/4544‑5114                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                              gctgcGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTNAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGNTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCCTTTGAATCCAGGCGAatat                                                                                                                                                                                                                                                                                          >  2:532224/1‑300 (MQ=255) gctgcGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCCGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCAGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAatat                                                                                                                                                                                                                                                                                          >  2:429810/1‑300 (MQ=255)    gcGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGA‑CAGTTGAATCCAGGCGAATATGGCt                                                                                                                                                                                                                                                                                      <  1:17146/300‑1 (MQ=255)          aaCGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTc                                                                                                                                                                                                                                                                                 >  1:15582/1‑300 (MQ=255)             gTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTc                                                                                                                                                                                                                                                                              >  1:556818/1‑300 (MQ=255)               gAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGGCGTGAGTCAGCCGAATAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGCACTGG‑‑GCTACACCTAGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGTTTGTTCCTc                                                                                                                                                                                                                                                                              >  2:175624/1‑298 (MQ=255)                aaCGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGc                                                                                                                                                                                                                                                                           <  1:66350/300‑1 (MQ=255)                              aCGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCAc                                                                                                                                                                                                                                                                         <  1:242690/288‑1 (MQ=255)                                         acgacgGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCTCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAag                                                                                                                                                                                                                                                  <  2:681120/300‑1 (MQ=255)                                           gacgGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCNGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTAGGCACCGCGCATCCGGCGAAATTTAAaga                                                                                                                                                                                                                                                 >  2:547038/1‑299 (MQ=255)                                                         aCCCAAAGCGACTCAGTCGACGTTATCCGACGTTATGGACGTGAGTCAGCCAAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCAGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCGCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGCGACCAGTTGGATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAAGTGAAAGAGAGCGTGGAAg                                                                                                                                                                                                                                      <  2:52609/296‑1 (MQ=255)                                                                   aCTCATGCGACGTTATCCAACGCGATGGACGTTATTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGGTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGTCGTAGTGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTGAAAGAGAGCGGGGAAGCGATTCGCggg                                                                                                                                                                                                                           <  2:35060/297‑2 (MQ=255)                                                                        ggCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTAGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGtt                                                                                                                                                                                                                   >  2:98946/1‑300 (MQ=255)                                                                            aCGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGAt                                                                                                                                                                                                               >  1:66236/1‑300 (MQ=255)                                                                            aCGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGAt                                                                                                                                                                                                               >  2:302252/1‑300 (MQ=255)                                                                            aCGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTAGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGAt                                                                                                                                                                                                               >  2:418494/1‑300 (MQ=255)                                                                              gTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATc                                                                                                                                                                                                              <  1:547038/299‑1 (MQ=255)                                                                                 aTCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGcc                                                                                                                                                                                                          >  1:690784/1‑300 (MQ=255)                                                                                          gatagACGTGAGTCAGCCGAACAACTGTCCGCGTGTGTAAGAGTCGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGTTTATTCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGTAGCCGCGCGCTGTCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCGTGGTCCTCGGCACCGCGCAGCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTGGGAGCTGCCAAAagag                                                                                                                                                                                                   <  2:285000/294‑1 (MQ=255)                                                                                             ggACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTAGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTAGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCCGCCAAAAGAACTGGc                                                                                                                                                                                              >  2:82524/1‑300 (MQ=255)                                                                                              gACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCa                                                                                                                                                                                             >  1:330122/1‑300 (MQ=255)                                                                                               aCGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTgtca                                                                                                                                                                                             >  1:413308/1‑296 (MQ=255)                                                                                               aCGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCa                                                                                                                                                                                             >  2:414042/1‑299 (MQ=255)                                                                                                       cAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCt                                                                                                                                                                                   >  2:562586/1‑300 (MQ=255)                                                                                                         gCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAACGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAACAGAGCTGGCAG‑AACGTGCTGa                                                                                                                                                                                 >  1:271120/1‑300 (MQ=255)                                                                                                         gCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGa                                                                                                                                                                                 >  2:84384/1‑300 (MQ=255)                                                                                                               ccacTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCAGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCAAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGACCTGCAGAAACAGCTGGCGG‑GACGTGATGATTTAcc                                                                                                                                                                           >  2:244783/3‑300 (MQ=255)                                                                                                               ccacTGGCCGCGTGCGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTACAAGAACTGG‑‑GCTACACCTAGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGTCACCGCGCCTCCTGCGCACTTTAAAGCGCGCGTGGAAGCGATTCTCGGTGAACCGTGGGATCTGCACAAAGAGCTGGCAG‑AATGTGCTGATTTAcc                                                                                                                                                                           >  2:244784/3‑300 (MQ=255)                                                                                                               caacTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGCGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTAcc                                                                                                                                                                           >  2:244769/1‑300 (MQ=255)                                                                                                               caacTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGAGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTAcc                                                                                                                                                                           >  2:245500/1‑300 (MQ=255)                                                                                                               caacTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTAGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAAGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAACCTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGATCTGGCAG‑AAAGTGCTGATTTa                                                                                                                                                                             >  2:245198/1‑298 (MQ=255)                                                                                                                          gtgtGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCTGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTACCCTTGCTTTcac                                                                                                                                                                >  2:19476/1‑300 (MQ=255)                                                                                                                          gtgtGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTACCCTTGCTTTcac                                                                                                                                                                >  1:695311/1‑300 (MQ=255)                                                                                                                          gtgtGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAAAGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTACCCTTGCTTTcac                                                                                                                                                                >  2:18539/1‑300 (MQ=255)                                                                                                                               gAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGGGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGGAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTACCCTTGCTTTCACATAAt                                                                                                                                                           <  1:115170/300‑1 (MQ=255)                                                                                                                                aagagtggtccagcaacAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCATGGATGATTAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTTG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCGGATTTACCCTTGCGTTCACATaagc                                                                                                                                                          <  2:92945/284‑3 (MQ=255)                                                                                                                                  gagTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGTCAG‑AACGTGCTGATTTACCCTTGCTTTCACATAATCt                                                                                                                                                         >  1:399746/1‑299 (MQ=255)                                                                                                                                  gagTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTACCCTTGCTTTCACATAATCt                                                                                                                                                         >  2:167016/1‑299 (MQ=255)                                                                                                                                  gagTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTACCCTTGCTTTCACATAATCt                                                                                                                                                         >  1:399736/1‑299 (MQ=255)                                                                                                                                                            aCTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTaa                                                                                                                              >  1:424246/1‑300 (MQ=255)                                                                                                                                                                   gagCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCAGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATtgat                                                                                                                        >  2:651885/1‑299 (MQ=255)                                                                                                                                                                        gggTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGAT‑‑G‑‑aa                                                                                                              >  2:444163/1‑300 (MQ=255)                                                                                                                                                                        gggTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGAT‑‑G‑‑aa                                                                                                              >  1:647877/1‑300 (MQ=255)                                                                                                                                                                         ggTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGAT‑‑G‑‑Aat                                                                                                             >  1:70923/1‑300 (MQ=255)                                                                                                                                                                                  gcagTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTCAATTGATGAT‑‑G‑‑AATCAT‑‑CAGTaa                                                                                                  >  1:43146/4‑300 (MQ=255)                                                                                                                                                                                  gCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCNGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGAT‑‑G‑‑AATTCT‑‑CAGTaa                                                                                                  >  2:513844/1‑300 (MQ=255)                                                                                                                                                                                  gCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCNGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGAT‑‑G‑‑AATCAT‑‑CAGTaa                                                                                                  >  2:513714/1‑300 (MQ=255)                                                                                                                                                                                  gCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGAT‑‑G‑‑AATCAT‑‑CAGTaa                                                                                                  >  2:590944/1‑300 (MQ=255)                                                                                                                                                                                  gCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTT‑CTGCGTTGCGTAAATTGATGAT‑‑G‑‑AATCAT‑‑CAGTaaa                                                                                                 >  1:37414/1‑300 (MQ=255)                                                                                                                                                                                  gCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTAGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGAACAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAGCGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGAT‑‑G‑‑AATCAT‑‑CAGTaa                                                                                                  >  1:42767/1‑300 (MQ=255)                                                                                                                                                                                            atgaAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGTAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGAT‑‑G‑‑AATCAT‑‑CAGTAAAATCTATTCa                                                                                        <  2:582662/300‑1 (MQ=255)                                                                                                                                                                                               aaaCCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGAT‑‑G‑‑AATCAT‑‑CAGTAAAATCTATTCAT‑‑Ta                                                                                   >  1:569095/1‑300 (MQ=255)                                                                                                                                                                                                    aCGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCTTAGCGTATCGTGCGCTGCGTGACCATTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAATAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTACCCTTGCTTGCACATAATCTGCCCGCCGATTGTGCTGCGTTGCGTAAAGTGATGAT‑‑G‑‑AATCAT‑‑CAGTAAAAGCGATTCAtgagcgg                                                                                 <  2:354281/299‑7 (MQ=255)                                                                                                                                                                                                    aCGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCTTAAATTGATGAT‑‑G‑‑AATCAT‑‑CAGTAAAATCTATTCAT‑‑TATCTCa                                                                              >  1:291263/1‑300 (MQ=255)                                                                                                                                                                                                        aaCAGACAATGCGTGAGTTAAAAGAACTGT‑‑GCTACACCTCGGAGCCGCACGCTTCCGTAGCGTATCGTGCGCTGCGTGACCAGTGGAATCCAGGCGAATATTGCTTGTTCCGCGGCACCGCGCATCCGGCGANATTTAAAGAGAGCGTGGAAGCGATTCTTGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCGGATTTATCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGAT‑‑G‑‑AATCAT‑‑CAGTAAAATCTATTCAT‑‑TATCTCaagc                                                                           <  2:218746/299‑3 (MQ=255)                                                                                                                                                                                                            gACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACCTAGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGAT‑‑G‑‑AATCAT‑‑CAGTAAAATCTATTCAT‑‑TATCTAAATCAGGcc                                                                      >  1:261266/1‑300 (MQ=255)                                                                                                                                                                                                                       aGTTAAAAGAACTGGCNGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAGGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGCG‑‑AATCATGNCAGTAAAATCTATTCATGNTATCTCAATCAgcggc                                                                     >  1:575844/1‑295 (MQ=255)                                                                                                                                                                                                                       aGTTAAAAGAACTGGCNGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAAAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAGGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGAT‑‑GCCAATCATGNCAGTAAAATCTATTCATGNTATCTCAATc                                                                           >  1:575849/1‑293 (MQ=255)                                                                                                                                                                                                                       aGTTAAAAGAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCGGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAATCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTAACCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGAT‑‑G‑‑AATCAT‑‑CAGTAAAATCTATTCAT‑‑TATCTCAATCAGGCCGGGTTTGCtt                                                            <  1:481874/299‑1 (MQ=255)                                                                                                                                                                                                                               gAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGAT‑‑G‑‑AATCAT‑‑CAGTAAAATCTATTCAT‑‑TATCTCAATCAGGCCGGGTTTGCTTTTATGCAGc                                                   >  2:680132/1‑300 (MQ=255)                                                                                                                                                                                                                               gAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGAT‑‑G‑‑AATCAT‑‑CAGTAAAATCTATTCAT‑‑TATCTCAATCAGGCCGGGTTTGCTTTTATGCAGc                                                   >  1:83664/1‑300 (MQ=255)                                                                                                                                                                                                                               gAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGAT‑‑G‑‑AATCAT‑‑CAGTAAAATCTATTCAT‑‑TATCTAAATCAGGCCGGGTTTGCTTTTATGCAGc                                                   >  1:455822/1‑300 (MQ=255)                                                                                                                                                                                                                               gAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTACCCTTGCTTTCACATAATCTGCACGCCGATTTTGCTGCGTTGCGTAAATTGATGAT‑‑G‑‑AATCAT‑‑CAGTAAAATCTATTCAT‑‑TATCTCAATCAAGCCGGGTTTGCTTTTATGCAGc                                                   >  2:680181/1‑300 (MQ=255)                                                                                                                                                                                                                               gAACTGG‑‑GCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCAGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGAT‑‑G‑‑AATCAT‑‑CAGTAAAATCTATTCAT‑‑TATCTCAATCAGGCCGGGTTTGCTTTTATGCAGc                                                   <  1:651885/300‑1 (MQ=255)                                                                                                                                                                                                                                    gg‑‑gCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGAT‑‑G‑‑AATCAT‑‑CAGTAAAATCTATTCAT‑‑TATCTCAATCAGGCCGGGTTTGCTTTTCTGCAGccggct                                              >  1:651860/1‑296 (MQ=255)                                                                                                                                                                                                                                    gg‑‑gCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGAT‑‑G‑‑AATCAT‑‑CAGTAAAATCTATTCAT‑‑TATCTCAATCAGGCCGGGTTTGCTTTTATGCAGccggct                                              >  1:234255/1‑296 (MQ=255)                                                                                                                                                                                                                                    gg‑‑gCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTNGCGTAAATTGATGAT‑‑G‑‑AATCAT‑‑CAGTAAAATCTATTCAT‑‑TATCTCAATCAGGCCGGGTTTGCTTTTATGCAGccggct                                              >  1:513506/1‑296 (MQ=255)                                                                                                                                                                                                                                            cacctaggAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑GACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGAT‑‑G‑‑AATCCT‑‑CAGTAAAATCTATTCAT‑‑TATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTa                                       >  2:472276/7‑300 (MQ=255)                                                                                                                                                                                                                                              cctCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGAT‑‑G‑‑AATCAT‑‑CAGTAAAATCTATTCAT‑‑TATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTAt                                      >  2:652183/3‑299 (MQ=255)                                                                                                                                                                                                                                                           cGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTATCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGAT‑‑G‑‑AATCAT‑‑CAGTAAAATCTATTCAT‑‑TATTTCAATCAGGCAGGGTTGGCTTTTCTGCAGCC‑AGCTTTTTTATGAAGAAAtcctgaa                        >  2:570519/1‑294 (MQ=255)                                                                                                                                                                                                                                                               gCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGAT‑‑G‑‑AATCAT‑‑CAGTAAAATCTATTCAT‑‑TATCTCAATCAGGAAGGGTTTGCTTTTATGC‑GGCAGGCTTTTTTATGAAGAAATTATGGAGaaa                    >  2:97099/1‑300 (MQ=255)                                                                                                                                                                                                                                                               gCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGAT‑‑G‑‑AATCAT‑‑CAGTAAAATCTATTCAT‑‑TATCTAAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGaaa                    >  2:190710/1‑300 (MQ=255)                                                                                                                                                                                                                                                                    agcgtATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGTTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGAT‑‑G‑‑AATCAT‑‑CAGTAAAATATATTCAT‑‑TATCTCAATCAGGCCGGGTTTGCTTTTATGTAGCC‑GGCTTTTTTATGAAGAAATTATGGAGaaaaaaga               >  2:274380/5‑297 (MQ=255)                                                                                                                                                                                                                                                                    agcgtATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGAT‑‑G‑‑AATCAT‑‑CAGTAAAATCTATTCAT‑‑TATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGaaaaacga               >  1:390965/5‑297 (MQ=255)                                                                                                                                                                                                                                                                     gcgtATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGAT‑‑G‑‑AATCAT‑‑CAGTAAAATCTATTCAT‑‑TATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGAc              <  1:429810/297‑1 (MQ=255)                                                                                                                                                                                                                                                                       gtATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAAAGTTGGATCTGCAAAAAGAGCTGGCAG‑AACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGAT‑‑G‑‑AATCAT‑‑CAGTAAAATCTATTCAT‑‑TATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGaaacacgaca             >  2:25786/2‑292 (MQ=255)                                                                                                                                                                                                                                                                       gtATCATGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTAGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCAAAAAGAGCTGGCAG‑AACGTGCTGATTTAACCTTGCTTTCACATAATCTGCACGCCGATTTTGCTGCGTTGCGTAAATTGATGAT‑‑G‑‑AATCAT‑‑CAGTAAAATCTATTCAT‑‑TATCTAAATCAGGCCGGGTTTGCTTTTATGCAGCA‑GGCTTTTTTATGAAGAAATTATGAAGaaaaacga               >  2:617693/2‑294 (MQ=255)                                                                                                                                                                                                                                                                                 cTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGACACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTACCCTTGCTTTCACATAATCTGCACGCCGATTTTGCTGCGTTGCGTAAATTGATGAT‑‑G‑‑AATCAT‑‑CAGTAAAATCTATTCAT‑‑TATCTCAATCAGGCAGGGTTTGCTTTTATGTAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGAAAGGGaaaaa     >  2:157757/1‑297 (MQ=255)                                                                                                                                                                                                                                                                                 cTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGAT‑‑G‑‑AATCAT‑‑CAGTAAAATCTATTCAT‑‑TATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGga  >  1:65643/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                              GCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGG‑‑GCTACACTTCGGAGCCGCACGCTGCCGTAGCTTATCGTGCGCTGCGTGATCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAG‑AACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGAT‑‑G‑‑AATCAT‑‑CAGTAAAATCTATTCAT‑‑TATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGA  >  NC_000913/4544‑5114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑