Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,614,170	C→T	F230F (TTC→TTT)	yjjV →	putative metal‑dependent hydrolase YjjV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,614,170	0	C	T	100.0%	201.4 / NA	60	F230F (TTC→TTT)	yjjV	putative metal‑dependent hydrolase YjjV
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/33);  total (27/33)

TGCATTCACGGCGCACGCACGACAAACTGGCGATGCATCTTAAACGCCACGATTTACCGCGCACTGGCGTGGTTCACGGTTTTTCCGGCAGCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCC‑GTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTC  >  NC_000913/4613875‑4614447                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                       tGCATTCACGGCGCACGCACGACAAACTGGCGATGCATCTTAAACGCCACGATTTACCGCGCACTGGCGTGGTTCACGGTTTTTCCGGCAGCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGcc                                                                                                                                                                                                                                                                                     >  1:163460/1‑299 (MQ=255)               acgcacgACAAACTGGCGATGCATCTTAAACGCCACGATTTACCGCGCACTGGCGTGGTTCACGGTTTTTCCGGCAGCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGAGACGGATATGCAGCTCAAAGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGt                                                                                                                                                                                                                                                                        >  2:57080/1‑298 (MQ=255)                        aaaCTGGCGATGCATCTTAAACGCCACGATTTACCGCGCACTGGCGTGGTTCACGGTTTTTCCGGCAGCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGa                                                                                                                                                                                                                                                              <  1:553786/299‑1 (MQ=255)                         aaCTGGCGATGCATCTTAAACGCCACGATTTACCGCGCACTGGCGTGGTTCACGGTTTTTCCGGCAGCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAAc                                                                                                                                                                                                                                                            <  1:686777/300‑1 (MQ=255)                                     aTCTTAAACGCCACGGTTTACCGCGCACTGGCGTGGTTCACGGTTTTTCCGGCAGCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAGTTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGGTATTGCTGGAAACCGACGCGCCGGATATGCAGCTCAACGGTTTTCAGGGGCAGCCCAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTTCGCCGGGAACCGGCAGATga                                                                                                                                                                                                                                                  >  2:47918/1‑298 (MQ=255)                                     aTCTTAAACGCCACGATTTACCGCGCACTGGCGTGGTTCACGGTTTTTCCGGCAGCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATgag                                                                                                                                                                                                                                                 <  1:428848/299‑1 (MQ=255)                                            gcGCCACGATTTATCGCGCCCTGTCGTTGTTCACTGTTTTTCCGGCAGCCTGCAACATGCCAAACGATTTGTACAGCTGGGCTACAAAACTGGCTTAGGCGATACTATCACCTATCCACGCACCATTAAAACCCGCGGTGTCATCGCAAAATCACCGCTTGCACCGTTATTGCTGGAAACCGACTCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTgcgc                                                                                                                                                                                                                                          <  2:296434/298‑1 (MQ=255)                                            aCGCCACGATTTACCGCGCACTGGCGTGGTTCACGGTTTTTCCGGCAGCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCa                                                                                                                                                                                                                                         <  1:648524/300‑1 (MQ=255)                                                           gcgcACTGGCGTGGTTCACGTTTTTTCCTGCAGCCTGCGACAGGCCGAACGGTTTGTACAGCTGGGCGACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCACTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGCTGCTtaataa                                                                                                                                                                                                                          <  2:195857/300‑1 (MQ=255)                                                           gcgcACTGGCGTGGTTCACGGTTTTTCCGGCAGCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTtaataa                                                                                                                                                                                                                          <  1:571865/300‑1 (MQ=255)                                                               aCTGTCGTGGTTCACGTTTCTCCCGGCAGCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCGATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAACTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAacac                                                                                                                                                                                                                       <  2:566758/299‑1 (MQ=255)                                                               aCTGGCGTGGTTCACGGTTTTTCCTGCAGCCTGCAGCAGTCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTTATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCTGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACg                                                                                                                                                                                                                      <  2:74976/300‑1 (MQ=255)                                                               aCTGGCGTGATTCACGGTTTTTCCGGCAGCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTATGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCGCAACGGTTTTCATGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAacac                                                                                                                                                                                                                       <  2:566921/299‑1 (MQ=255)                                                                  gggggggTTCACGGTTTTTCCGGCAGCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATACCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCACGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGTGCCGGGAACTGGCGGATGAGATTGCGCAAATGTTGCTTAATCACACGtat                                                                                                                                                                                                                   >  2:14770/6‑300 (MQ=255)                                                                  ggCGTGGTTCACGGTTTTTCCGGCAGCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCACGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATCACCCGtat                                                                                                                                                                                                                   >  2:13580/1‑300 (MQ=255)                                                                  ggCGTGGTTCACGGTTTTTCCGGCAGCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCAACTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCAGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCAGGAGCAGGTTGCCCGTGTGTTTGCCGTGTTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATCGCGCAAGCATTGCTCAATAACACgca                                                                                                                                                                                                                    >  2:14477/1‑297 (MQ=255)                                                                     gttgTTCACGGTTTTTCCGGCAGTCTGCAACCGGCCGAACTGTTTGTACAGCTGGGCTATAAAATCGGCGGAGGCGGTACTAGCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATCACCACCGACATCGTTATTGCTGGAAACCGACTCGCCGGATATGCCGTTCAACGGTTTTCAGGTGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTACTCTGCGAGTGGCGCCTGGAACCGGCGGATGAGACTgcgc                                                                                                                                                                                                                                          <  2:296668/271‑1 (MQ=255)                                                                     gTGGTTCACGGTTTTTCCGGCAGCCTGCAACAGGCCGAACTGTTTGTACAGCTGTGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATAc                                                                                                                                                                                                                 <  2:134762/299‑1 (MQ=255)                                                                      tGGTTCACGGTTTTTCCGGCAGCCTGCAACAGGCCGAACGGTTTGCACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACgt                                                                                                                                                                                                               <  1:77132/300‑1 (MQ=255)                                                                      tGGTTCACGGTTTGTCCTGCAGCCGGCAACAGGCCGAACGGGTTGTACAGCTGGGCGACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGGAAAACCCGCGATGTCATCGCAAAATTACCGCTTGCATCGTTATTGCTGGAAACCGACGCGCCGNATATGCCGCTCAACGGTTTTCATGGGCAGCCTAACCGCCCGGAGCATGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACgt                                                                                                                                                                                                               <  2:502504/300‑1 (MQ=255)                                                                        gTTCACGGTTTTTCCGGCAGCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACgtt                                                                                                                                                                                                              >  1:391344/1‑299 (MQ=255)                                                                        gTTCACGGTTTTTCCGGCAGCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGGTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGTGTTGCTTAATACCACGTATACgtt                                                                                                                                                                                                              >  2:177702/1‑299 (MQ=255)                                                                               gTTTTTCCGGCAGCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACg                                                                                                                                                                                                      <  2:679730/300‑1 (MQ=255)                                                                                           gCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTCTACGTTGTTTAACGTGCA‑GTAGGcc                                                                                                                                                                                          >  2:135592/1‑299 (MQ=255)                                                                                           gCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCC‑GTAGGcc                                                                                                                                                                                          >  2:670311/1‑299 (MQ=255)                                                                                             cTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGAt                                                                                                                                                                                                                                               <  2:418339/245‑1 (MQ=255)                                                                                             cTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCC‑GTAGGCCGGa                                                                                                                                                                                       >  1:482239/1‑300 (MQ=255)                                                                                               gCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCC‑GTAGGCCGGATa                                                                                                                                                                                     <  1:259430/300‑1 (MQ=255)                                                                                                             ggTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCC‑GTAGGCCGGATAAGGCGTTCAcgcc                                                                                                                                                                        >  1:206389/1‑299 (MQ=255)                                                                                                                ttttACAGCTGTGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCC‑GTAGGCCGGATAAGGc                                                                                                                                                                                 <  1:298265/284‑1 (MQ=255)                                                                                                                ttGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCC‑GTAGGCCGGATAAGGc                                                                                                                                                                                 <  1:296385/287‑1 (MQ=255)                                                                                                                ttGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCC‑GTAGGCCGGATAAGGc                                                                                                                                                                                 >  2:298265/1‑287 (MQ=255)                                                                                                                ttGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCA‑GTAGGCCGGATAAGGcatgtctattatac                                                                                                                                                                    >  2:296385/1‑287 (MQ=255)                                                                                                                ttGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATAAGTTGTTTAACGTGCC‑GTAGGCCGGATAAGGCGTTCACGCCGCa                                                                                                                                                                     >  2:646721/1‑299 (MQ=255)                                                                                                                  gTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCC‑GTAGGCCGGATAAGGCGTTCACGCCGCATcc                                                                                                                                                                  >  1:463099/1‑300 (MQ=255)                                                                                                                  gTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCAGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTGTAACGTGCCCGTAGGc                                                                                                                                                                                           >  2:578281/1‑276 (MQ=255)                                                                                                                                     ttGGCGTCGTCGGTACTATCACCTATCCACGCGTCAGTAAAACCCGCGATGTCATCGCACAATTACCGCTGTCCTCGTTATTGCTGGCAACCGACGCGCCGGATCTGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGTTCCCCGGCGGATGCGATTGCGCAAGCTTTTCTTCATAACACGTATACGTTGTTTAACGTGCC‑GTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGc                                                                                                                                               <  2:327413/300‑1 (MQ=255)                                                                                                                                     ttGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCC‑GTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGc                                                                                                                                               <  1:26358/300‑1 (MQ=255)                                                                                                                                      tGGCGTAGGAGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTTGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCC‑GTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGc                                                                                                                                               <  2:327431/299‑1 (MQ=255)                                                                                                                                                 gTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCC‑GTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGc                                                                                                                                   >  2:689853/1‑300 (MQ=255)                                                                                                                                                   aCTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCC‑GTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCtt                                                                                                                                 >  1:183953/1‑300 (MQ=255)                                                                                                                                                   aCTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTGGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCC‑GTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCtt                                                                                                                                 >  2:564933/1‑300 (MQ=255)                                                                                                                                                   aCTATCAACTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCC‑GTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCtt                                                                                                                                 >  1:81857/1‑300 (MQ=255)                                                                                                                                                      aTCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCC‑GTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCACAATGCCTGATGCGACGCTTAAc                                                                                                                              >  1:554517/1‑300 (MQ=255)                                                                                                                                                        cACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTNCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCNCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCC‑GTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAAcgc                                                                                                                            >  2:534672/1‑300 (MQ=255)                                                                                                                                                                              aCCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGAGATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCGTTGCGAGTTGCGTCGGGAACCGGCGGATGAGATTGCACAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCC‑GTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACaag                                                                                                         <  1:13580/297‑3 (MQ=38)                                                                                                                                                                                  gcgATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCC‑GTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGc                                                                                                  <  1:229097/300‑1 (MQ=255)                                                                                                                                                                                               aaaaTTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCC‑GTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGcc                                                                                     <  1:534672/300‑1 (MQ=255)                                                                                                                                                                                                   ttACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCC‑GTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGAt                                                                                 <  1:564933/300‑1 (MQ=255)                                                                                                                                                                                                        gCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCGTAATAACACGTATACGTTGTTTAACGTGCC‑GTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGc                                                                            <  1:199632/300‑1 (MQ=255)                                                                                                                                                                                                                cGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCC‑GTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCAcgc                                                                    >  1:283839/1‑300 (MQ=255)                                                                                                                                                                                                                 gTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCC‑GTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCAcgcc                                                                   <  1:653034/300‑1 (MQ=255)                                                                                                                                                                                                                            aaaCCGACGCGCCGGATATGCCGCTCAACGGTTTTCATGGGCAGCCTAACCGCCCTGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCC‑GTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAg                                                        <  2:329841/300‑1 (MQ=255)                                                                                                                                                                                                                                         ggATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCC‑GTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAAt                                            <  1:520089/299‑1 (MQ=255)                                                                                                                                                                                                                                                  gCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCC‑GTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGc                                   >  1:187847/1‑299 (MQ=255)                                                                                                                                                                                                                                                                      aGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCC‑GTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCa              >  1:674046/1‑300 (MQ=255)                                                                                                                                                                                                                                                                       gCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCC‑GTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCAt             >  2:657786/1‑300 (MQ=255)                                                                                                                                                                                                                                                                           aaCCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCC‑GTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCt         <  1:422627/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                   ggAGCATGCTGCCTGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATATGTTGTTTAACGTGCC‑GTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATNCGACGCTTAACGCGTCTCATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACaggtt  <  2:497155/299‑5 (MQ=255)                                                                                                                                                                                                                                                                                   ggAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCC‑GTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACaggtt  <  1:57080/299‑5 (MQ=18)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                       TGCATTCACGGCGCACGCACGACAAACTGGCGATGCATCTTAAACGCCACGATTTACCGCGCACTGGCGTGGTTCACGGTTTTTCCGGCAGCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCC‑GTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTC  >  NC_000913/4613875‑4614447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑