BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,556,917	A→G	E2G (GAA→GGA)	idlP →	iraD leader peptide

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,556,917	0	A	G	98.3%	384.4 / NA	120	E2G (GAA→GGA)	idlP	iraD leader peptide
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (50/68); minor base T (0/2); total (50/70)
Fisher's exact test for biased strand distribution p-value = 5.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

TTTACAAACTAATTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGAAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCAC  >  NC_000913/4556626‑4557209

tttACAAACTAATGGTTTCAAACAATACATGGCTGACTATGCGGAAATAAATAACTTCCCACCGGAATTAAGTAGCAGCGTTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGGGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTTAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATTGGAAATGAGc                                                                                                                                                                                                                                                                                              <  2:411892/300‑1 (MQ=255)

      aaCTAATTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAAt                                                                                                                                                                                                                                                                                        >  1:667257/1‑300 (MQ=255)

          aaTTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAg                                                                                                                                                                                                                                                                                    <  2:27868/300‑1 (MQ=255)

          aaTTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAg                                                                                                                                                                                                                                                                                    <  1:552475/300‑1 (MQ=255)

             tGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTNTCCTGAAAAGTCAGTAGATATTCATAGGCAAGNAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAgtgg                                                                                                                                                                                                                                                                                 <  2:532504/300‑1 (MQ=255)

             tGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAAGTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAgtgg                                                                                                                                                                                                                                                                                 <  2:319191/300‑1 (MQ=255)

             tGTTTCAAATAATACATGGCGGATTATGCGTAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTNGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAg                                                                                                                                                                                                                                                                                    <  2:222627/297‑1 (MQ=255)

             tGTTTCAAATAATACATGGCCGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAgtgg                                                                                                                                                                                                                                                                                 <  2:265450/300‑1 (MQ=255)

                   aaaTAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGccc                                                                                                                                                                                                                                                                            <  1:173127/299‑1 (MQ=255)

                   aaaTAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGca                                                                                                                                                                                                                                                                             >  2:422812/1‑297 (MQ=255)

                   aaaTAAGACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGccc                                                                                                                                                                                                                                                                            <  1:473813/299‑1 (MQ=255)

                       aataCATTGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGgtgt                                                                                                                                                                                                                                                                       <  1:254944/300‑1 (MQ=255)

                       aataCATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGgtgt                                                                                                                                                                                                                                                                       <  1:440140/300‑1 (MQ=255)

                       aataCATGGCTGATTATGCGGAAATAAAGAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGgtgt                                                                                                                                                                                                                                                                       <  1:440130/300‑1 (MQ=255)

                        ataCATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGtt                                                                                                                                                                                                                                                                      >  2:595234/1‑300 (MQ=255)

                            aTGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATTGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAgg                                                                                                                                                                                                                                                                  <  2:338972/300‑1 (MQ=255)

                            aTGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAgg                                                                                                                                                                                                                                                                  <  1:688581/300‑1 (MQ=255)

                            aTGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTAAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCa                                                                                                                                                                                                                                                                    >  1:208419/1‑298 (MQ=255)

                                   aTTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGcc                                                                                                                                                                                                                                                           <  1:258551/300‑1 (MQ=255)

                                   aTTAGGCGGAAATAAATAATCTCCCACCTGAATTAGGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGGTTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGcc                                                                                                                                                                                                                                                           <  1:258499/300‑1 (MQ=255)

                                        gCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCata                                                                                                                                                                                                                                                      <  1:121183/300‑1 (MQ=255)

                                         gggAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGc                                                                                                                                                                                                                                                                <  1:240917/288‑1 (MQ=255)

                                          ggAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCNGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATg                                                                                                                                                                                                                                                    <  1:513001/300‑1 (MQ=255)

                                          ggAAATAAATAATTCCCCACCGGAATTAATTATCAGCGTGGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCatat                                                                                                                                                                                                                                                     <  1:6672/299‑1 (MQ=255)

                                           gAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGt                                                                                                                                                                                                                                                   <  2:557938/300‑1 (MQ=255)

                                                    aatTCCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACg                                                                                                                                                                                                                                          <  1:84832/300‑1 (MQ=255)

                                                            acctgAATTAAGTAGCAGCGTTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTTAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAg                                                                                                                                                                                                                                   <  2:277879/295‑1 (MQ=255)

                                                            aCCGGAATTAAGTAGCAGTGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAg                                                                                                                                                                                                                                   <  1:108424/299‑1 (MQ=255)

                                                            aCCGGAATTAAGTAGCAGCTGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAg                                                                                                                                                                                                                                   <  1:108469/299‑1 (MQ=255)

                                                            aCCGGAATTAAGTAGCAGCGTTGACAAGTATTTTCATCCACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATTGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAg                                                                                                                                                                                                                                   <  2:405045/299‑1 (MQ=255)

                                                            aCCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAg                                                                                                                                                                                                                                   <  2:481669/299‑1 (MQ=255)

                                                            aCCGGAATTAAGTAGCAACGGTGACAAGTATTTTCATCTACTTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGNAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAg                                                                                                                                                                                                                                   <  2:221451/299‑1 (MQ=255)

                                                                gAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATg                                                                                                                                                                                                                                                    >  2:72703/1‑278 (MQ=255)

                                                                gAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATg                                                                                                                                                                                                                                                    <  1:72703/278‑1 (MQ=255)

                                                                gAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAANCATGACTTTTTTCCTGAAAAGTCAGTAGATATNCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTAAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTgcgc                                                                                                                                                                                                                              >  2:531407/1‑300 (MQ=255)

                                                                     aattAGCAGCGGTGACAAGTATTTTCCTCTACTTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGTAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAat                                                                                                                                                                                                                         <  2:378578/297‑1 (MQ=255)

                                                                      aGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAat                                                                                                                                                                                                                         >  1:476625/1‑299 (MQ=255)

                                                                           cagcGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATNAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGc                                                                                                                                                                                                                    >  1:153777/1‑299 (MQ=255)

                                                                            agcGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGa                                                                                                                                                                                                                  <  1:659866/300‑1 (MQ=255)

                                                                               ggTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTAAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGTACGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGGTGCGACaa                                                                                                                                                                                                               >  2:95067/1‑300 (MQ=255)

                                                                                 tGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATc                                                                                                                                                                                                             <  1:282365/300‑1 (MQ=255)

                                                                                     aaGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACtt                                                                                                                                                                                                         <  1:398316/300‑1 (MQ=255)

                                                                                       gTATTTTCATCTACGTAACTATTCGGAATCTTCAGCATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCACATCATGACTCTTTTCCTGAAAAGTCAGTAGATATTCATCGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGAATATGTTGCTAAACGTCAGGAGTGCGCAAAATGCTGCGACAATTACTTCa                                                                                                                                                                                                       >  2:464353/1‑300 (MQ=255)

                                                                                       gTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCa                                                                                                                                                                                                       >  2:464345/1‑300 (MQ=255)

                                                                                           tttCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTTAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCt                                                                                                                                                                                                   <  2:439836/300‑1 (MQ=255)

                                                                                                tCTACTTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGtttt                                                                                                                                                                                              <  1:529261/300‑1 (MQ=255)

                                                                                                tCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGtttt                                                                                                                                                                                              <  1:485349/300‑1 (MQ=255)

                                                                                                   aCGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCNTAGGCAAGTAAGGTTTTATACTTTGCTGACAGNATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGtttt                                                                                                                                                                                              >  2:533949/1‑297 (MQ=255)

                                                                                                   aCGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTAcc                                                                                                                                                                                           <  1:47928/300‑1 (MQ=255)

                                                                                                   aCGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGNGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTAcc                                                                                                                                                                                           >  1:148971/1‑300 (MQ=255)

                                                                                                   aCGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTAAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGAAATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGACGCGAAAATCAATTAAGGCTGTTTTAcc                                                                                                                                                                                           >  1:280376/1‑300 (MQ=255)

                                                                                                        aCTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAACCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGaaa                                                                                                                                                                                      >  2:72547/1‑300 (MQ=255)

                                                                                                                    atTCAGAGTATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTTATAGGCAAGTCGGGTTCTATACTTTGCTGACAGGATTCAGGCCTGTCTAAGACTGACATGGATGTAATGAACAAAAGGGAATAGCTATGGGAAATGAGCATCAATACAGTGGTGCACGGTGTTCAGGGCAAGCCGCATATGTTGCTCAACGTCAGGAGTGCGCAAAACGATGCGACCATCACtt                                                                                                                                                                                                         >  2:644000/1‑269 (MQ=255)

                                                                                                                    atTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCAGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGTGCAAAGTGATGCGACAATCACTTCAGGTTGTTTTACCTGAAATTTCAGGCAATa                                                                                                                                                                          >  2:280127/1‑300 (MQ=255)

                                                                                                                         gAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAAc                                                                                                                                                                      >  1:391278/1‑299 (MQ=255)

                                                                                                                           atatACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTc                                                                                                                                                                   >  1:412618/1‑300 (MQ=255)

                                                                                                                                     gttttttttttGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCatat                                                                                                                                                                                                                                                     <  2:680378/206‑1 (MQ=255)

                                                                                                                                          ttttttGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATc                                                                                                                                                    <  1:262146/300‑1 (MQ=255)

                                                                                                                                                  gTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAgtgg                                                                                                                                                                                                                                                                                 <  1:170966/167‑1 (MQ=255)

                                                                                                                                                  gTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAgtgg                                                                                                                                                                                                                                                                                 <  1:169877/167‑1 (MQ=255)

                                                                                                                                                  gTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAgtgg                                                                                                                                                                                                                                                                                 <  1:169758/167‑1 (MQ=255)

                                                                                                                                                  gTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAgtgg                                                                                                                                                                                                                                                                                 >  2:170966/1‑167 (MQ=255)

                                                                                                                                                  gTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAgtgg                                                                                                                                                                                                                                                                                 >  2:169877/1‑167 (MQ=255)

                                                                                                                                                  gTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAgtgg                                                                                                                                                                                                                                                                                 <  1:169265/167‑1 (MQ=255)

                                                                                                                                                  gTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTAAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAgtgg                                                                                                                                                                                                                                                                                 >  2:169265/1‑167 (MQ=255)

                                                                                                                                                      gatgatTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTTACAGGATT‑AGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAATGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCGAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGa                                                                                                                                        <  1:332245/299‑1 (MQ=255)

                                                                                                                                                             tttATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAATTAAGGTTTTATACTTTGCTTACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCGTATGTTGCTAAACGTTAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATctcccc                                                                                                                                 <  1:602080/300‑3 (MQ=255)

                                                                                                                                                             tttATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATctcctc                                                                                                                                 <  2:428500/300‑1 (MQ=255)

                                                                                                                                                                                              aGTAGATATTCATAGGCAATTAATGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCg                                                                                                <  2:313513/300‑1 (MQ=255)

                                                                                                                                                                                                     atTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGGATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGATGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTAc                                                                                         >  2:605470/1‑300 (MQ=255)

                                                                                                                                                                                                      ttCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACt                                                                                        >  1:419481/1‑300 (MQ=255)

                                                                                                                                                                                                            ggCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTAAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTAATTCGGCATTCCCGTCATTACTTGttt                                                                                   >  2:87322/1‑299 (MQ=255)

                                                                                                                                                                                                              cAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCa                                                                                 >  1:191665/1‑299 (MQ=255)

                                                                                                                                                                                                                aGTAAGTTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGc                                                                              <  2:436155/300‑1 (MQ=255)

                                                                                                                                                                                                                 gTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACGTGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCCGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCa                                                                             <  2:40239/300‑1 (MQ=255)

                                                                                                                                                                                                                     ggTTTTATACTTTGCTGACAGGATTCATGTCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACNATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGcc                                                                         <  2:496973/300‑1 (MQ=255)

                                                                                                                                                                                                                       tttggtcttttGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCa                                                                             <  1:233805/286‑1 (MQ=255)

                                                                                                                                                                                                                                  gCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACACAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGCTGCGACACTCACTTCAGGCTGTTTTACCTGAACTTTCAGGCCATAAAAAGTCCTCGCTGCGTAGCTCGGTATGCAGCGATCTCCTCACTCTTTTTCATTCTCATCATTCGGCATTACCGt                                                                                               >  2:94151/1‑265 (MQ=255)

                                                                                                                                                                                                                                  gCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGt                                                             >  2:92741/1‑299 (MQ=255)

                                                                                                                                                                                                                                     gACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTAAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTAATTGTTTCAGGCATGCACGAGTGGCAAGTGCAt                                                         >  2:257000/1‑300 (MQ=255)

                                                                                                                                                                                                                                         ggATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAAcc                                                     <  1:492150/300‑1 (MQ=255)

                                                                                                                                                                                                                                         ggATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTNCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAAcc                                                     <  2:548377/300‑1 (MQ=255)

                                                                                                                                                                                                                                           aTTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCGGAGTGGCAAGTGCATAAccc                                                    >  2:459422/1‑299 (MQ=255)

                                                                                                                                                                                                                                           aTTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAAcc                                                     >  1:213852/1‑298 (MQ=255)

                                                                                                                                                                                                                                           aTTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTAAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTAACTCTTTTTAATTCTCCTCATTCGGCATTACCTTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAAccc                                                    >  2:693768/1‑299 (MQ=255)

                                                                                                                                                                                                                                           aTTCAGGCCTGTCTAAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAAccc                                                    >  1:213794/1‑299 (MQ=255)

                                                                                                                                                                                                                                                     gTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTAATTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCTAGCGACAAAc                                         >  2:84056/1‑300 (MQ=255)

                                                                                                                                                                                                                                                     gTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTAACTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCCTTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCAGAGCGACAAAc                                         >  2:343498/1‑300 (MQ=255)

                                                                                                                                                                                                                                                     gTCTCAGACTGACATAGATGTAATGAACAAAAAGGAATGGCTATGGGAAATGAGCATCCATACAGTGGTGCCCGGTGTTCAGGGCAAGCAGCACATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCAATTCAGGCTGTTTTCCCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATTCAGCGATCTCCTCCCTCTTTTTAATTCTCCTCATTCGGCATTACCGTCACTAACTGTTTCAGGCATGCCCAAGTGGAAAGTGCATaa                                                       >  2:343478/1‑286 (MQ=255)

                                                                                                                                                                                                                                                           gACTGACATGGATGTANTGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGCCCTTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCNAGTGGCAAGTGCATAACCNGAGCGACAAACATCTcc                                   >  1:580260/1‑300 (MQ=255)

                                                                                                                                                                                                                                                           gACTGACATGGATGTANTGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCNAGTGGCAAGTGCATAACCNGAGCGACAAACATCTcc                                   >  1:580550/1‑300 (MQ=255)

                                                                                                                                                                                                                                                               gACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCagag                               <  2:580550/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                aCATGGATGTAATGAACAAAAGGGAATGGCTATGGTAAAGGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGNTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGc                              <  2:208419/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                aCATGGATGGAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCagag                               <  2:580260/299‑1 (MQ=255)

                                                                                                                                                                                                                                                                   tGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCt                             >  1:596148/1‑298 (MQ=255)

                                                                                                                                                                                                                                                                                     gggAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTACAGAGCTGGTATTGCCGTCAGCTGCGt         >  2:45846/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                                       gAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTACAGAGCTGGTATTGCCGTCAGCTGCGTTc       >  2:399321/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                                            gCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCAc  <  1:422812/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                                            gCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAAAGCTGGTATTGCCGTCAGCTGCGTTCTGCAc  >  2:430654/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                                            gCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCCCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTTGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCAc  <  2:12386/300‑1 (MQ=255)

TTTACAAACTAATTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGAAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCAC  >  NC_000913/4556626‑4557209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`