Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,553,609	T→A	L237I (TTA→ATA)	uxuB →	D‑mannonate oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,553,609	0	T	A	100.0%	270.8 / NA	85	L237I (TTA→ATA)	uxuB	D‑mannonate oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (39/46);  total (39/46)

ACTGGATCTCAATAACCCGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTG‑AAAGCGT‑TTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACCTCGGCGGCTATGAAACCATTGCCGACACCGTGACTAACCCGGCTTATCGC  >  NC_000913/4553320‑4553872                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                          aCTGGATCTCAATAACCCGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCGGCGTCGTGAAAAAGGGTTG‑AAAGCGT‑TTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAAt                                                                                                                                                                                                                                                                 >  2:684609/1‑300 (MQ=255)                   gCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCTTCGTGAAAAAGGGTTG‑AAAGCGT‑TTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGgtgt                                                                                                                                                                                                                                              <  1:417010/300‑1 (MQ=255)                   gCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTG‑AAAGCGT‑TTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTggg                                                                                                                                                                                                                                                 <  1:620937/297‑1 (MQ=255)                   gCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTG‑AAAGCGT‑TTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGgtgt                                                                                                                                                                                                                                              <  1:18289/300‑1 (MQ=255)                      gctCAAGCATGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTG‑AAAGCGT‑TTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑CACAGCTGGGTGttt                                                                                                                                                                                                                                            >  2:429748/3‑299 (MQ=255)                      gATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTG‑AAAGCGT‑TTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTa                                                                                                                                                                                                                                           >  1:210943/1‑300 (MQ=255)                      gATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTG‑AAAGCGT‑TTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑AACAGCTGGGTGTTTa                                                                                                                                                                                                                                           >  2:95328/1‑300 (MQ=255)                      gATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTG‑AAAGCGT‑TTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCAACTTCCAGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGttt                                                                                                                                                                                                                                            >  1:332105/1‑299 (MQ=255)                          aaGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTG‑AAAGCGT‑TTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGAc                                                                                                                                                                                                                                       <  1:67416/300‑1 (MQ=255)                          aaGCACGAGCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTG‑AAAGCGT‑TTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGAc                                                                                                                                                                                                                                       <  1:67520/300‑1 (MQ=255)                           aGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTG‑AAAGCGT‑TTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGAcc                                                                                                                                                                                                                                      <  1:95277/300‑1 (MQ=255)                           aGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTG‑AAAGCGT‑TTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGAcc                                                                                                                                                                                                                                      >  2:464331/1‑300 (MQ=255)                                   cTGGAAAACCCGACTGCGCCGAAGTCCGCGCTTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTG‑AAAGCGT‑TTACGGTGATGTCCTGCGCTAACGTGCGTGAACACGGTCATGTGGCGAAGGTCGCGTTCCTGGGGCTGGCTCAGGCGCGTGACCCGCAGTTGGCGTAATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGCCCTCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTAc                                                                                                                                                                                                                                          >  2:162819/1‑288 (MQ=255)                                   cTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTG‑AAAGCGT‑TTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGAACCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTgcgc                                                                                                                                                                                                                               >  2:162524/1‑299 (MQ=255)                                                 tGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTG‑AAAGCGT‑TTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAGGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAAc                                                                                                                                                                                                                 <  1:60645/299‑1 (MQ=255)                                                 tGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTG‑AAAGCGT‑TTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAAc                                                                                                                                                                                                                 <  1:59936/299‑1 (MQ=255)                                                 tGCGCCGAAGTCCGCGATTGGTGACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTG‑AAAGCGT‑TTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGTTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAAc                                                                                                                                                                                                                 <  1:60422/299‑1 (MQ=255)                                                       gAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTG‑AAAGCGT‑TTACGTTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTc                                                                                                                                                                                                            <  1:412069/298‑1 (MQ=255)                                                       gAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTG‑AAAGCGT‑TTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTc                                                                                                                                                                                                            <  1:412063/298‑1 (MQ=255)                                                          gTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTG‑AAAGCGT‑TTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCa                                                                                                                                                                                                       >  1:205106/1‑300 (MQ=255)                                                           tCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTG‑AAAGCGT‑TTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑g                                                                                                                                                                                                     <  2:671632/300‑1 (MQ=255)                                                              gcgATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTG‑AAAGCGT‑TTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑Gt                                                                                                                                                                                                    <  1:545334/298‑1 (MQ=255)                                                                   tGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTG‑AAAGCGT‑TTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACTAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑ATGGGTGAt                                                                                                                                                                                             >  2:79634/1‑300 (MQ=255)                                                                   tGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTG‑AAAGCGT‑TTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGAt                                                                                                                                                                                             >  1:411273/1‑300 (MQ=255)                                                                         cATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTG‑AAAGCGT‑TTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGa                                                                                                                                                                                       >  2:659625/1‑300 (MQ=255)                                                                             tgacgcggttttgctgcncgtcgtGAAAAAGGGTTGNAAAGCGTGTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCACGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCANGTGGGTGATTGAAGAt                                                                                                                                                                                      <  1:581072/282‑1 (MQ=255)                                                                             gtcgAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTG‑AAAGCGT‑TTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAAc                                                                                                                                                                                   >  2:409808/1‑300 (MQ=255)                                                                             gtcgAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTG‑AAAGCGT‑TTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAAc                                                                                                                                                                                   <  1:667310/300‑1 (MQ=255)                                                                                                gtcgtGAAAAAGGGTTGNAAAGCGTGTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCACGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCANGTGGGTGATTGAAGAt                                                                                                                                                                                      <  1:581058/281‑1 (MQ=255)                                                                                                   gtGAAAAAGGGTTG‑AANGCGT‑TTACGGTGATGTCCNGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGCTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGNTAATGGTCGCCCGGAt                                                                                                                                                              <  1:592483/299‑1 (MQ=255)                                                                                                   gtGAAAAAGGGTTG‑AAAGCGT‑TTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCAGGAtt                                                                                                                                                             >  2:448795/1‑300 (MQ=255)                                                                                                        aaaGGGTTG‑AAAGCGT‑TTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGAt                                                                                                                                                        <  1:54297/300‑1 (MQ=255)                                                                                                            ggTTG‑AAAGCGT‑TTACGTTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATTTGGCGAAGGTCGCGGTACTGGGGCTTGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGTTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAg                                                                                                                                                    <  1:474536/300‑1 (MQ=255)                                                                                                            ggTTG‑AAAGCGT‑TTACGTTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAg                                                                                                                                                    <  1:16/300‑1 (MQ=255)                                                                                                            ggTTG‑AAAGCGT‑TTACGGTGATGTCCTGCGATAACATGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAg                                                                                                                                                    <  1:204952/300‑1 (MQ=255)                                                                                                             gTTG‑AAAGCGT‑TTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAg                                                                                                                                                    <  1:262438/299‑1 (MQ=255)                                                                                                              ttG‑AAAGCGT‑TTACGGTGATGTCCTGCGATAACGTGCTTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCATAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTATTTACGACCCGTGCGCCATTGCCTGCGAACCATTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAg                                                                                                                                                    <  2:480261/298‑1 (MQ=255)                                                                                                                     gCGT‑TTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑AACAGCTGGGTGTTTACGAACCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTAGTTAATGGTCGCCAGGATTGGGATAAAGTGGGCGca                                                                                                                                            >  2:619228/1‑300 (MQ=255)                                                                                                                      cGT‑TTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTTGGATAAAGTGGGCGcac                                                                                                                                           >  1:426444/1‑300 (MQ=255)                                                                                                                        t‑ttACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAg                                                                                                                                         <  1:25678/300‑1 (MQ=255)                                                                                                                        t‑ttACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAg                                                                                                                                         <  2:466997/300‑1 (MQ=255)                                                                                                                             cGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCAAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTc                                                                                                                                      >  2:598465/1‑299 (MQ=255)                                                                                                                               gTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTTGTGCTGGCTCAGGCGCGTGACNCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGtt                                                                                                                                   <  1:130495/300‑1 (MQ=255)                                                                                                                               gTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGtt                                                                                                                                   <  2:409519/300‑1 (MQ=255)                                                                                                                                 gATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGc                                                                                                                                 <  1:411022/300‑1 (MQ=255)                                                                                                                                 gATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑AACAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGc                                                                                                                                 <  1:680233/300‑1 (MQ=255)                                                                                                                                        tGCGATAACGTGCGTGAAAACGGTCATGTTGCGAAGGTCGCGGTACTGGTGCTGGCTCAGGCGCGTGACCCGCAGCTGGCTGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACg                                                                                                                            <  2:614074/298‑1 (MQ=255)                                                                                                                                        tGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACg                                                                                                                            <  2:132619/298‑1 (MQ=255)                                                                                                                                         gCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCACGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGtt                                                                                                                          >  2:320723/1‑299 (MQ=255)                                                                                                                                            aTAACGTGCGTGAAAACGGTCATGTGTCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGc                                                                                                                      <  2:287645/300‑1 (MQ=255)                                                                                                                                                         aaaCGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGTTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAgctgtctcttatacacatctcgc                                                                                                         <  1:191140/300‑23 (MQ=255)                                                                                                                                                              gTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTT‑ACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCt                                                                                                   >  1:94825/1‑300 (MQ=255)                                                                                                                                                                   gtgGCGAAGGTCGCGGTACTGTGGCTTGCCCAGGCGCGTTACCCGCAGCTGGCAGCATGGATTGAAGAAAATGTCACCTGCCCGTGCACCATGGTGGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTACTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGGGATTTAAGATAACTTCGTTAATGGTCACCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAAGGATGAAGCTGCGt                                                                                               <  2:274312/300‑1 (MQ=255)                                                                                                                                                                        gAAGGTCGCGGTACTGGGGCTTGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGTAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGTTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGc                                                                                           <  2:345067/299‑1 (MQ=255)                                                                                                                                                                            gTCGCGGTGCTGGGGCTGGGTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAAc                                                                                      <  2:109607/300‑1 (MQ=255)                                                                                                                                                                                  gtccTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGc                                                                                >  1:352628/4‑300 (MQ=255)                                                                                                                                                                                  gTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGNCGCCAGAAACCATACAGGAAATTGCTG‑ACCAGNTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCACGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAGATGATGAAGCTGCGTATGCTGAACGGCAGc                                                                                >  2:526530/1‑300 (MQ=255)                                                                                                                                                                                  gTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGNTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAGTGATGAAGCTGCGTATGCTGAACGGCAGc                                                                                >  2:513484/1‑300 (MQ=255)                                                                                                                                                                                  gTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGNTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGc                                                                                >  2:511874/1‑300 (MQ=255)                                                                                                                                                                                  gTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTCCGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGc                                                                                >  1:6518/1‑300 (MQ=255)                                                                                                                                                                                  gTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGACATGATGAAGCTGCGTATGCTGAACGGCAGc                                                                                >  2:573056/1‑300 (MQ=255)                                                                                                                                                                                  gTACTAGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCAGTGTACCATGGTTGACCGCATCGTTCCGGTGGCGACGCCAGACATCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCACGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTCTGCTGAACGGCAga                                                                                >  2:57418/1‑299 (MQ=255)                                                                                                                                                                                  gTACTAGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCAACTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTA‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAAATTAGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCAAAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGc                                                                                >  2:418437/1‑300 (MQ=255)                                                                                                                                                                                   tACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGcc                                                                               >  2:565728/1‑300 (MQ=255)                                                                                                                                                                                    aCTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCa                                                                              >  1:463093/1‑300 (MQ=255)                                                                                                                                                                                       ggggCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCActc                                                                           <  1:286594/300‑1 (MQ=255)                                                                                                                                                                                             ggctCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTGCACC‑GCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCNGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTTGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGCTGAAGCTGTGTATGCTGAACGGCAGCCACTCTTTTCt                                                                     >  2:214406/1‑300 (MQ=255)                                                                                                                                                                                             ggctCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGCTAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCAATCTTTTCt                                                                     >  1:34198/1‑300 (MQ=255)                                                                                                                                                                                             ggctCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCAACTTCCCGTGCACCATAATTGAACGCATCGTTCCGGCGGCGACGCCAGCAACCATACAGGAAATTGCTA‑ACCAGCTGGGTATTTACGACCCGTGCGCCATTACCTGCGAACCGTTCNGTCA‑GCGGGTGACTGAAGATCACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGCTCGAAATGATGAAGCTGCGTACGCTGAACAGCAGACACTCTTTTCt                                                                     >  2:214358/1‑300 (MQ=255)                                                                                                                                                                                                tCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTgg                                                                   <  1:464331/299‑1 (MQ=255)                                                                                                                                                                                                tCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTgg                                                                   <  2:415982/299‑1 (MQ=255)                                                                                                                                                                                                     cgcgTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCCTTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTAcc                                                             >  2:591240/1‑300 (MQ=255)                                                                                                                                                                                                     cgcgTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTAcc                                                             >  1:531736/1‑300 (MQ=255)                                                                                                                                                                                                        gTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTc                                                           <  1:294093/299‑1 (MQ=255)                                                                                                                                                                                                                cAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGAAGCCAGAAACCATACAGGAAATTGCTG‑ACCAGTTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTACTGGTCGCCCGGATTGGGATAAAGTGGGCGCCCAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGCTGAAGCTGCGTATGCTGAACGGCAGCCCATCTTTTATGGCGTACCTCGGTTAcc                                                    >  2:90940/1‑298 (MQ=255)                                                                                                                                                                                                                           aTGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCANGTGGGTGATTGAAGAt                                                                                                                                                                                      <  1:581081/158‑1 (MQ=255)                                                                                                                                                                                                                                          tGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACCTCGGCGGCTATGAAACCATTGCCGacac                        <  1:635864/300‑1 (MQ=255)                                                                                                                                                                                                                                              aCCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACCTCGGCGGCTATGAAACCATTGCCGACACCGt                     >  2:621906/1‑299 (MQ=255)                                                                                                                                                                                                                                                 ttCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACCTCGGCGGCTATGAAACCATTGCCGACACCGTGAc                  <  1:11767/299‑1 (MQ=255)                                                                                                                                                                                                                                                   cccGTGCACCATGGTTGACCGCCTCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACCTCGGCGGCTATGAAACCATTGCCGACACCGTGACTaa               >  2:678720/1‑300 (MQ=255)                                                                                                                                                                                                                                                         cACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACCTCGGCGGCTATGAAACCATTGCCGACACCGTGACTAACCCGGc         >  2:680089/1‑300 (MQ=255)                                                                                                                                                                                                                                                         cACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACCTCGGCGGCTATGAAACCATTGCCGACACCGTGACTAACCCGGc         >  1:97148/1‑300 (MQ=255)                                                                                                                                                                                                                                                          aCCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACCTCGGCGGCTATGAAACCATTGCCGACACCGGGACTAACCCGGCt        <  2:569656/300‑1 (MQ=255)                                                                                                                                                                                                                                                             aTGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACCTCGGCGGCTATGAAACCATTGCCGACACCGTGACTAACCCGGCtt       <  1:684609/298‑1 (MQ=255)                                                                                                                                                                                                                                                                gTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACCTCGGCGGCTATGAAACCATTGCCGacctgtctcttatacacatctcgcag  <  1:79634/300‑25 (MQ=255)                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                          ACTGGATCTCAATAACCCGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTG‑AAAGCGT‑TTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTG‑ACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCA‑GTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACCTCGGCGGCTATGAAACCATTGCCGACACCGTGACTAACCCGGCTTATCGC  >  NC_000913/4553320‑4553872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑