Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,420	T→A	A229A (GCT→GCA)	thrC →	threonine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,420	0	T	A	98.8%	248.4 / ‑5.1	81	A229A (GCT→GCA)	thrC	threonine synthase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base A (39/41);  total (39/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCGCGACCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTG‑TTGCGCAGCTGCCGCAGGA‑GACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGT  >  NC_000913/4124‑4714                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                         aCCGCGACCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑tt                                                                                                                                                                                                                                                                                                      >  1:625017/1‑300 (MQ=255)      gACCTCCGGTGATACCGGAGCGGCATTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCNACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTgcgc                                                                                                                                                                                                                                                                                                  <  1:508233/299‑1 (MQ=255)       aCCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAg                                                                                                                                                                                                                                                                                                <  2:443288/300‑1 (MQ=255)       aCCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTAAGGTTTACCGAATGTGAAAGTGGTTATCCTATATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCa                                                                                                                                                                                                                                                                                                 >  1:68121/1‑299 (MQ=255)              gTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCAATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGc                                                                                                                                                                                                                                                                                         >  2:421961/1‑300 (MQ=255)                      ggAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGgaag                                                                                                                                                                                                                                                                                   <  1:665769/298‑3 (MQ=255)                                 ggCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTANCATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCt                                                                                                                                                                                                                                                                     >  1:515210/1‑300 (MQ=255)                                 ggCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGTTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCt                                                                                                                                                                                                                                                                     >  2:259344/1‑300 (MQ=255)                                 ggCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTAAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCt                                                                                                                                                                                                                                                                     >  2:259994/1‑300 (MQ=255)                                   cTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCt                                                                                                                                                                                                                                                                     >  2:461123/1‑298 (MQ=255)                                          tttCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCG‑AGGA‑AGCGCGCAACCAGCTGGTTGtctc                                                                                                                                                                                                                                                            <  1:597809/299‑1 (MQ=255)                                          tttCTACGGTTTACCGAAGGTGAAAGTGGTTGTCCTCTATCCACTAGGCAAAATTAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCTGCAATATTTAAACTGTTTCCAGCGACTGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGGTTGAGAGCTGAAAGTGGCGCTGGGGTTGAACTCAGCTAACTCGAGTAACATCAGTCGGTTGCTGGCGCAGGGTTGCTGCTACTTTGGAGCAG‑TTGTGCAGCTGCCTAAGGA‑AGCGCGCAACCAGCTGGTTGtctc                                                                                                                                                                                                                                                            <  1:597800/300‑1 (MQ=255)                                           ttCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGTAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGAAGGCGATTTCGATGCATGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGAAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGtct                                                                                                                                                                                                                                                             >  1:101006/1‑298 (MQ=255)                                           ttCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATTATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCCGCAGTTTGCTGGCGCAGATTTGCTACTAATTTGAAGCAG‑TTGCGCAGCTGCCGCAGGAAGA‑GCGCAAACAGCTAGTTGtct                                                                                                                                                                                                                                                             >  2:295349/1‑298 (MQ=255)                                           ttCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGtct                                                                                                                                                                                                                                                             >  2:294825/1‑298 (MQ=255)                                           ttCTAAGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAAACAGCTGGTTGtctc                                                                                                                                                                                                                                                            >  2:391039/1‑299 (MQ=255)                                                 ggTTTACCGAATGTGAAAGTGGTTCTCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAACCTCAGCTAACTCGATTAACATCAGCAGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTTTCGGTGcaa                                                                                                                                                                                                                                                     >  2:455543/1‑298 (MQ=255)                                                 ggTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATAAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGcaa                                                                                                                                                                                                                                                     >  2:455524/1‑298 (MQ=255)                                                          gaTGTAAAAGTGGTTATCCTTTATCCACGATGCAAAGTCATTCCACTGCAAGAAAAACTGCTCCTTACACTGTGCTGCAAGACCGAAGCTGTTGCCGTCGACGGCGATGTCGAGGCCTGTCAGGCGCTGGGGGAGTAGGCGGTTGATGATGAAGAGCTGAAGGGGGCGCTGGGGGGAAACGCAGCTAACTCGATTAACATTAGCCGTTTGCTTGCGCAGATTTGCCACTGCTTT‑TGGTTGGTTGCGCGGTTGTCGTAGGA‑GGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAAc                                                                                                                                                                                                                                            <  2:670827/299‑1 (MQ=255)                                                               gAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACtt                                                                                                                                                                                                                                          >  2:242690/1‑297 (MQ=255)                                                                     ggTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAAACAGCTGGTTGTCTAGGTGCCAAGCGGAAACTTCGGCGAttt                                                                                                                                                                                                                                 >  2:19968/1‑300 (MQ=255)                                                                      gTTATCCTCTATCCCCGGGGCAACATTAGTCCACTGCAAGAAAACCTGTTTTGTACATTGGACGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGCAGCAGGCGTTTGATGCTGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAATTCGATTACCATCAGCAGTTTGCTGGCGCAGAGTTGCTACTACTTTGAAGCAG‑CTGCGCAGCGGCCGAAGGA‑GACGTGCAACCAACTGGTTGTATAGGTGTCAAGCGGAGACTTCggtgcttt                                                                                                                                                                                                                                 >  2:94352/1‑293 (MQ=255)                                                                      gTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGACAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTAATACTTTGAAGCAG‑TTGCGCAGCTACCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTAGGTGCCAAGCGGAAAATTAGGCGAt                                                                                                                                                                                                                                   >  2:92380/1‑297 (MQ=255)                                                                      gTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGAttt                                                                                                                                                                                                                                 >  2:32978/1‑299 (MQ=255)                                                                           ccTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCTCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGAcggc                                                                                                                                                                                                                           >  1:681120/1‑300 (MQ=255)                                                                           ccTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGAcggc                                                                                                                                                                                                                           >  2:461663/1‑300 (MQ=255)                                                                             tctATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGAcggc                                                                                                                                                                                                                           >  1:471974/1‑298 (MQ=255)                                                                             tctATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTAGGTGCCAAGCGGAAACTTCGGCGATTTGAcggc                                                                                                                                                                                                                           >  1:104481/1‑298 (MQ=255)                                                                                             aaTCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTc                                                                                                                                                                                                         <  1:383970/300‑1 (MQ=255)                                                                                                 aGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACt                                                                                                                                                                                                      <  1:35720/299‑1 (MQ=255)                                                                                                       cTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGCCGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCAGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTAGGCGATTTGACGGTGGGTCTGCTGGCGAAGTCAACCGGTCt                                                                                                                                                                                                >  1:215488/1‑299 (MQ=255)                                                                                                           aagagaaatttttcttgacatttgtcggcAATATCTAAAATGTTTCCATCGACAGCAATTGCGATGCCTCTCAGGCGCTGGTGAAGCAGGGGTTTGATGATGAAGAGCTGAAATTGGCGCTGTGGTTAAACTCAGCTAACTCGCTTAACATCAGCCGTTTGCTGNCGCAGATTTGCTACTACTTTGAAGTAG‑TTGCGNAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCgg                                                                                                                                                                                           <  2:526695/275‑1 (MQ=255)                                                                                                                     gTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTAGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTATGCCGGTGAAGCGttt                                                                                                                                                                                 >  2:186046/1‑300 (MQ=255)                                                                                                                       tCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTACCGGTGAAACGTTTTa                                                                                                                                                                               >  2:121433/1‑300 (MQ=255)                                                                                                                       tCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGAAGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTa                                                                                                                                                                               >  2:40176/1‑300 (MQ=255)                                                                                                                       tCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTAGATGCCCAGCGGACACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTATGCCGGTGAAACGTTTTa                                                                                                                                                                               >  2:122144/1‑300 (MQ=255)                                                                                                                          gTACATTGGTCGGCAATATCGAAACTTTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGTTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATtg                                                                                                                                                                            <  1:661200/300‑1 (MQ=255)                                                                                                                          gTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATtg                                                                                                                                                                            <  1:661157/300‑1 (MQ=255)                                                                                                                          gTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTAGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTATGCCGGTGAAACGTTTTAtt                                                                                                                                                                             >  2:66350/1‑299 (MQ=255)                                                                                                                            aCATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATtgct                                                                                                                                                                          >  1:52609/1‑300 (MQ=255)                                                                                                                            aCATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCATGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTNGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAATCGGTCTGCCGGTGAAACGTTTTATtgct                                                                                                                                                                          >  2:542898/1‑300 (MQ=255)                                                                                                                                    cggcAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTAGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTAGGTCTGCCGGTGAAACGTTTTATTGTTGCGACCaa                                                                                                                                                                  >  2:572/1‑300 (MQ=255)                                                                                                                                            tCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACg                                                                                                                                                          <  1:468140/300‑1 (MQ=255)                                                                                                                                                aaCTGTTGCCATCGACTGCGATTTCGATGCCTGTCAGGCGCTGGTTAAACAGTCGTTGGATGATGAAGAGCTGAAATTGGCGCTGGGTTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCGGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGTA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGTCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTAGGTCTGGCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATAc                                                                                                                                                      <  1:256819/300‑1 (MQ=255)                                                                                                                                                aaCTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTTAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATAc                                                                                                                                                      <  1:460048/300‑1 (MQ=255)                                                                                                                                                aaCTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATAc                                                                                                                                                      <  1:448143/300‑1 (MQ=255)                                                                                                                                                 gcTGTTGCTATCGACGGCGATTTCGAGACCTGTCAGGCGCTGGTGAAGCATTCGTTTGATAATGAAGGGCTGAAAGTAGCACTGGGGTTAAACTCAGCGAACTCGATTAACATCAGCCTTTTACTTGCGCAGATTTGCTACTACTTTTAATCAG‑TTGCGCAGCTGCCGCAGTA‑AGCGCACAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACTGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTGTTATTGCTGCGACCAACGTGAACGATAcc                                                                                                                                                     <  2:149916/299‑1 (MQ=255)                                                                                                                                                 aCTGTTGCCATCGACGTCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTTAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGGGAACGATAc                                                                                                                                                      <  2:136409/299‑1 (MQ=255)                                                                                                                                                     ttGCCATCGACGTCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATAAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGc                                                                                                                                                 <  1:386327/300‑1 (MQ=255)                                                                                                                                                     ttGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCGGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTTGCGCTGGGGTTAAACTNAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGGTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGGGAACGATACCGTGc                                                                                                                                                 <  2:541159/300‑1 (MQ=255)                                                                                                                                                          aTCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGt                                                                                                                                            <  1:22180/300‑1 (MQ=255)                                                                                                                                                             gACGGCGATTTCGATGCCTGTCATGCGCTGTTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGttt                                                                                                                                          <  2:621481/299‑1 (MQ=255)                                                                                                                                                              aCGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTcc                                                                                                                                        <  1:61033/300‑1 (MQ=255)                                                                                                                                                              aCGGCGATTTCGATGCCGGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTcc                                                                                                                                        <  1:245684/300‑1 (MQ=255)                                                                                                                                                                ggCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTGGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTcc                                                                                                                                        <  1:245093/298‑1 (MQ=255)                                                                                                                                                                 gCGATTTCGATGCCTGTCATGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGTA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTcc                                                                                                                                        <  1:61216/297‑1 (MQ=255)                                                                                                                                                                   ggtttCGATGCCTGGCAGGCGCTGGTGAAGCAGGCGTTTGGTGATGAAGAGCTGAAGGTGGCGCTGGGGTTAAACTCAGCTAACTCGCTTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGTA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCac                                                                                                                                   <  2:680453/298‑1 (MQ=255)                                                                                                                                                                      ttCGATGCCTGTCATGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATGTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCacgac                                                                                                                                <  2:625017/300‑1 (MQ=255)                                                                                                                                                                               tGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAATTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGt                                                                                                                         <  1:691383/298‑1 (MQ=255)                                                                                                                                                                                gTCAGGCGCTGGTGAAGCAGGCGTTTGATGATTAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGt                                                                                                                      <  1:622427/300‑1 (MQ=255)                                                                                                                                                                                gTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGt                                                                                                                      <  1:289134/300‑1 (MQ=255)                                                                                                                                                                                     gcgcTGGTGAGTCAGGCGTTTGATGATGAGGGGCGGAGAGTTGCGTTTGGTTTAAATCCAGCTACCTCTATTAACAGCAGCCGTTTGCTGGCACAGATTTGCTACTCCTTGTAAGCAG‑TTTCACAACTGCCGCAGGA‑AACGCACAGCCGGCTGGTTGTCTCGGTGCTAAGCGGAAACGTCGGCAATTGGACGGCGGGTCGGCTGGCGAAGTCACTCGGTCTGCCGTTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGGCAGGGGTCAcc                                                                                                                  <  2:365985/299‑1 (MQ=255)                                                                                                                                                                                        cTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCaaa                                                                                                              >  1:35060/1‑300 (MQ=255)                                                                                                                                                                                          ggTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGc                                                                                                            >  1:218746/1‑300 (MQ=255)                                                                                                                                                                                          ggTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGATGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTAGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCCCGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGc                                                                                                            >  1:218803/1‑300 (MQ=255)                                                                                                                                                                                           gTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGTA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCg                                                                                                           <  1:668766/300‑1 (MQ=255)                                                                                                                                                                                           gTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCg                                                                                                           <  1:421961/300‑1 (MQ=255)                                                                                                                                                                                               agcaggtttttGATGATGAAGAGCTGAAATTGGCGCTTGGGTTAAACTCAGCTACCTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTTAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTTTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTTCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTc                                                                                                       <  2:438589/292‑1 (MQ=255)                                                                                                                                                                                                   ggCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGc                                                                                                   >  1:92945/1‑300 (MQ=255)                                                                                                                                                                                                             gatgaAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATcc                                                                                         <  1:538053/300‑1 (MQ=255)                                                                                                                                                                                                                  agagcTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAAATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAAcgc                                                                                    <  2:593516/296‑1 (MQ=255)                                                                                                                                                                                                                              ggCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAg                                                                        <  1:70455/300‑1 (MQ=255)                                                                                                                                                                                                                               gcgcTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGTAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGTTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGt                                                                       <  1:481792/300‑1 (MQ=255)                                                                                                                                                                                                                               gcgcTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGt                                                                       <  1:481875/300‑1 (MQ=255)                                                                                                                                                                                                                                     gggTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGcc                                                                  >  1:354281/1‑299 (MQ=255)                                                                                                                                                                                                                                           aaCTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTAGGTGCCCAGCGTACACTTAGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTCTTGCTGCGACCAAAGTGAACGATACCGTGCCACGCTTCCTGCACGACGGTCAGTGGTCAAACAAAGCGACTCCGGCAACGTTATCCACCTCGACGGACGTGAGTCAGCCGaacaaa                                                           >  2:17146/1‑299 (MQ=255)                                                                                                                                                                                                                                                    aaCTCGATTAACATCAGCTGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGTA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGTAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCcgcg                                                   <  2:600716/299‑1 (MQ=255)                                                                                                                                                                                                                                                        cGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCgtgt                                                >  1:285000/1‑298 (MQ=255)                                                                                                                                                                                                                                                                   agccgtctgcctgcccagagttaccactactTTGAAGCAG‑TTGCGCAGCCTCCGCAGGA‑AGCACACAACCAGCTGTTCGTCTCGGTGCCAAGCTGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGGCCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTc                                   <  2:66747/275‑1 (MQ=255)                                                                                                                                                                                                                                                                                    gATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCCAACGGAAACTTCGGAGATTTGAAGGCGGGTATGATGGAGAAGTCACTCGGTCTGACGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCAACGTTTACTGCACGACGGTCAGTGGTCAACCAAAGCGACTCAGGCGACGTTATCCAAGGCGAGGGACGTGAGTCGGCCGAACAACTGGACGAGTGTGGAAGAGTTATTCCGCAGCAAAATCTGGCa                  >  2:115170/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                     aaGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGt  <  1:186046/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                         ACCGCGACCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTG‑TTGCGCAGCTGCCGCAGGA‑GACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGT  >  NC_000913/4124‑4714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑