Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,516,378	G→A	N100N (AAC→AAT)	fecA ←	ferric citrate outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,516,378	0	G	A	100.0%	196.2 / NA	58	N100N (AAC→AAT)	fecA	ferric citrate outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/27);  total (31/27)

GTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGTGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGC  >  NC_000913/4516084‑4516672                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                       gTCCATCAGGACGGTCGAGCGGCTGGTGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTattatt                                                                                                                                                                                                                                                                                                   >  1:362283/1‑300 (MQ=255)    cATCAGGACGGTCGAGCGGCTGGTGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTAtttac                                                                                                                                                                                                                                                                                                >  1:354638/1‑298 (MQ=255)    cATCAGGACGGTCGAGCGGCTGGTGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTcc                                                                                                                                                                                                                                                                                                >  1:354224/1‑300 (MQ=255)     aTCAGGACGGTCGAGCGGCTGGTGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTTGCTGCCGTTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTcc                                                                                                                                                                                                                                                                                                <  2:45192/299‑1 (MQ=255)         ggACGGTCGAGCGGCTGGTGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGc                                                                                                                                                                                                                                                                                            >  1:75667/1‑299 (MQ=255)                      gctgttgAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAg                                                                                                                                                                                                                                                                              <  1:250615/294‑1 (MQ=255)                       cTGGTGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGt                                                                                                                                                                                                                                                                             >  1:23030/1‑300 (MQ=255)                              ggCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGc                                                                                                                                                                                                                                                                      <  2:585819/300‑1 (MQ=255)                                         aGGCCCCGGATGCCAGAGTTCATCGCCAGGTTGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCcagcag                                                                                                                                                                                                                                                            <  2:695116/299‑1 (MQ=255)                                         aGGCCCCGGATGCCAAAGTTCATCGCCAGTTCGTTGCTGCCATTGCCGTTGTTTTCCGGCGCGCTGGCGCCAGGGATGCGTTTAGGTACCTCACGCATGGTGGTTGCGCCTTTTTTGGCGAAATCCTCATGGCGGATCACGTCACGCGCGCCAACATGTTCAAATACGTCGTTTTCACGTGTATCACTCAGCCAGTCGCCGACCACGTTCAGGGCATCTTCTTTTGGGGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCcagcag                                                                                                                                                                                                                                                            <  2:695122/299‑1 (MQ=255)                                                ggATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGc                                                                                                                                                                                                                                                     <  1:538493/299‑1 (MQ=255)                                                 gATGCCAAGGTTCATCGCCAGGTCGTGGCTGCCGGTGCTGGTGTTGTTCGGCGCGCTGACGCCATGGATGCTGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGTTCAGGGTATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCCGCAGTCCGCTGCCGTCCAGCAGTTGTTGCa                                                                                                                                                                                                                                                    <  2:685391/299‑1 (MQ=255)                                                           ttCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCg                                                                                                                                                                                                                                         <  1:690955/300‑1 (MQ=255)                                                                 gCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGAcgtc                                                                                                                                                                                                                                    >  2:684075/1‑299 (MQ=255)                                                                                        gttgttTTCCGGCGCACTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTATTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGc                                                                                                                                                                                                             <  1:601691/299‑1 (MQ=255)                                                                                              ttCCGGCGCGCTGACGCCAGGGATGCGTTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCtt                                                                                                                                                                                                      <  1:250245/300‑1 (MQ=255)                                                                                                          gACGCCAGGGATGCGGTTAAGTACCTCACGCATGTTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACTTCTTTTTCACGCGCATCACCCAGCCAGTCACCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCTCATCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCtt                                                                                                                                                                                                      <  2:149306/288‑1 (MQ=255)                                                                                                            cGCCAGGGATGCGGTTNAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTcc                                                                                                                                                                                                                                                                           >  1:589325/1‑217 (MQ=255)                                                                                                            cGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTcc                                                                                                                                                                                                                                                                           <  2:589325/217‑1 (MQ=255)                                                                                                              ccAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCt                                                                                                                                                                                       >  1:360463/1‑299 (MQ=255)                                                                                                              ccAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCAGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCt                                                                                                                                                                                       >  2:354159/1‑299 (MQ=255)                                                                                                              ccAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCAACCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCt                                                                                                                                                                                       >  1:360478/1‑299 (MQ=255)                                                                                                                   gATGCGTTTAAGTACCTCACGCATGGGGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGAATCACGTCACGCGCGCCAGCATGTTCAAATACTTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGt                                                                                                                                                                                  <  2:32483/299‑1 (MQ=255)                                                                                                                       cGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAAcc                                                                                                                                                                             >  1:113938/1‑300 (MQ=255)                                                                                                                       cGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGAAGTCGTAATCGCAGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCaccc                                                                                                                                                                             >  2:180962/1‑297 (MQ=255)                                                                                                                         gTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCga                                                                                                                                                                           >  1:479254/1‑300 (MQ=255)                                                                                                                                 ccTCACGCATGGTGGTTGCGCCGGTTTTGGCGCAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTCTTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCCGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTaa                                                                                                                                                                   >  2:459990/1‑300 (MQ=255)                                                                                                                                      cGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTNTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATcc                                                                                                                                                               >  1:152644/1‑299 (MQ=255)                                                                                                                                              ggtTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGAAGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGCGGGTACATCCGCTGTGTGc                                                                                                                                                      >  2:128852/1‑300 (MQ=255)                                                                                                                                                 tGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGc                                                                                                                                                   <  1:422286/300‑1 (MQ=255)                                                                                                                                                         ttttGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTGCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTACTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACTTCGTAATCGCCGTGCAGGCCATTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGAt                                                                                                                                           <  2:14679/300‑1 (MQ=255)                                                                                                                                                              gCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCATTCCGCTGCCGTCCATCAGTTGTTTCATGCCGCTCTCTACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTTCCGCGTGTCAGGCTGGCGGCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAg                                                                                                                                       <  2:142710/299‑1 (MQ=255)                                                                                                                                                                     ccTCACGGCGGATCACNTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTANATCCGCTGTGTGCGGCATNCTGATTGAGCGCTTTAt                                                                                                                               >  1:581537/1‑300 (MQ=255)                                                                                                                                                                          cggcggATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCgcgc                                                                                                                          >  1:251897/1‑297 (MQ=255)                                                                                                                                                                                     gtcacgCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCATCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGGCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGc                                                                                                               <  2:360463/300‑1 (MQ=255)                                                                                                                                                                                     gtcacgCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGc                                                                                                               <  2:360478/300‑1 (MQ=255)                                                                                                                                                                                              gcCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTCTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTACCTCCCAGCGGTTTTACCTGCACTCCGCTGCCGTCCAGCATTTGTTGCAGGCCGCTCTCGCCTTCTCAATCCCCCTGCATACCTTTTCCCCCCTTTCCTCGCGGCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAg                                                                                                                                       <  2:475838/267‑1 (MQ=255)                                                                                                                                                                                                 aacATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAAcc                                                                                                     <  2:113938/296‑1 (MQ=255)                                                                                                                                                                                                 aGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCATGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCCCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGGCAGGCTGGCGGCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAAcc                                                                                                     <  2:105600/298‑1 (MQ=255)                                                                                                                                                                                                 aGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTNCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCTAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAAcc                                                                                                     <  1:511073/298‑1 (MQ=255)                                                                                                                                                                                                  gCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAAcc                                                                                                     <  2:362283/297‑1 (MQ=255)                                                                                                                                                                                                      gTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTgca                                                                                               >  1:388772/1‑299 (MQ=255)                                                                                                                                                                                                      gTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCAACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTAAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCAGGTGCGATATTAACCTGTgca                                                                                               >  1:388173/1‑299 (MQ=255)                                                                                                                                                                                                      gTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGAACACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGCCCAGCTATTATTTCCCAGCGGTTTTACCTGCCGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGAAGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGATGTGTGCGGCATACTGATTGAGCGCTTTATCGAACGATCCCGGTGCGATATTAACTTGTgca                                                                                               >  1:388767/1‑299 (MQ=255)                                                                                                                                                                                                                 tcgtTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTCTTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCCGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGTGGAATACTGATTGAGTGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGaa                                                                                   >  2:165828/1‑300 (MQ=255)                                                                                                                                                                                                                   gtTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGaaaa                                                                                 >  1:666178/1‑300 (MQ=255)                                                                                                                                                                                                                                tCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCNTACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGcc                                                                    >  1:503532/1‑300 (MQ=255)                                                                                                                                                                                                                                 cACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGACTGAGCGCTTTATCGAGCGATTCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGAACGGCCa                                                                   >  2:109930/1‑300 (MQ=255)                                                                                                                                                                                                                                              gCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCt                                                      <  2:633658/300‑1 (MQ=255)                                                                                                                                                                                                                                                 gACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACCGATTGAGCGCTTTACCGAGCGATCCAGGTGCGATGTCAACCTGTgca                                                                                               >  2:320034/1‑256 (MQ=255)                                                                                                                                                                                                                                                 gACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCAGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACCGACTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTCCCCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCa                                                                   >  2:320344/1‑284 (MQ=255)                                                                                                                                                                                                                                                       ggTCAGGGCATCTTCTTTTGGTGTGGGCGCGGGCTCCAGCGTCCAGCTACTATTTCCCAGCGGTTTTACCTGCAGTCCGCCGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGNCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGGGACCGGCAAGCGGCAGCAGGc                                                       >  2:226228/1‑290 (MQ=255)                                                                                                                                                                                                                                                                 tcttctTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAAcc                                   <  1:692080/300‑1 (MQ=255)                                                                                                                                                                                                                                                                   ttcttTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACAGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCaa                                 >  2:693729/1‑300 (MQ=255)                                                                                                                                                                                                                                                                     cttTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCaaa                                >  1:411104/1‑299 (MQ=255)                                                                                                                                                                                                                                                                      ttttGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGNTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAgg                              <  1:132859/300‑1 (MQ=255)                                                                                                                                                                                                                                                                      tttgggTGCTGGTGCGGGCTCCAGCGGCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCTAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAgg                              <  2:23030/296‑1 (MQ=255)                                                                                                                                                                                                                                                                                        cTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAAc            >  1:207736/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                  cAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGACAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAGCGGc  >  2:423840/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                       GTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGTGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGC  >  NC_000913/4516084‑4516672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑