Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,514,139	A→C	I57S (ATC→AGC)	fecB ←	ferric citrate ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,514,139	0	A	C	100.0%	204.2 / NA	60	I57S (ATC→AGC)	fecB	ferric citrate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (24/36);  total (24/36)

TGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGATGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTC  >  NC_000913/4513844‑4514421                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                           tGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGAc                                                                                                                                                                                                                                                                                        >  2:642943/1‑300 (MQ=255) tGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGAAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGAc                                                                                                                                                                                                                                                                                        >  1:543459/1‑300 (MQ=255)                    ttACCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTAAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCa                                                                                                                                                                                                                                                                     >  2:203153/1‑300 (MQ=255)                          accaTTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCTGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCgg                                                                                                                                                                                                                                                                 <  2:464377/298‑1 (MQ=255)                          accaTTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGAGCTGACGTCCACGGCGGCCAGCGCATCgg                                                                                                                                                                                                                                                                 <  2:446918/298‑1 (MQ=255)                           ccaTTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGa                                                                                                                                                                                                                                                              >  1:628782/1‑300 (MQ=255)                                     gatgatAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTc                                                                                                                                                                                                                                                    >  1:83987/1‑300 (MQ=255)                                           aGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCNATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGcac                                                                                                                                                                                                                                              <  1:512253/300‑1 (MQ=255)                                           aGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGcac                                                                                                                                                                                                                                              <  2:436043/300‑1 (MQ=255)                                           aGCCACAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCATGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACGTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGcac                                                                                                                                                                                                                                              <  2:435887/300‑1 (MQ=255)                                                   aTTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGGCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATcc                                                                                                                                                                                                                                      <  2:178813/300‑1 (MQ=255)                                                         aaTTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTtgt                                                                                                                                                                                                                                 >  2:456517/1‑299 (MQ=255)                                                                             ttGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAAc                                                                                                                                                                                                            <  1:42966/300‑1 (MQ=255)                                                                                     cttcagcagcAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCgt                                                                                                                                                                                                     >  1:561668/5‑299 (MQ=255)                                                                                        aaGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGTTCTGGTTTCAGAGCGGCAATGGCTTCCATGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTc                                                                                                                                                                                                 <  1:278033/300‑1 (MQ=255)                                                                                         agcagcAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCTTGTTc                                                                                                                                                                                                 >  1:257390/1‑299 (MQ=255)                                                                                           cagcagTACCGGCGCGATTTGCTGCAAGGCGATGTAACCCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCGGGTTTCAGAGTGGCAATGGCTTCCAAGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGTACTTCGGGCCGGATGCGTTTTGCGTCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCAAGCGCAGCGGCGAACGAGAGTTCCAGCACCAACATCCGTCGTGGCGTTTTTTCGAGTGTAACCGTGCCATGTTCGt                                                                                                                                                                                               >  2:261572/1‑299 (MQ=255)                                                                                           cagcagTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTc                                                                                                                                                                                              >  2:261527/1‑300 (MQ=255)                                                                                           cagcagTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTACACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTAGAGTGTAAACGTGCCGTGTTcttc                                                                                                                                                                                              >  2:262550/1‑297 (MQ=255)                                                                                            aacagTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGTTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTc                                                                                                                                                                                              <  2:484128/297‑1 (MQ=255)                                                                                                 tACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCTGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAAc                                                                                                                                                                                        <  2:40332/300‑1 (MQ=255)                                                                                                 tACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAAc                                                                                                                                                                                        <  1:274581/300‑1 (MQ=255)                                                                                                                     attcaatatAAACCCCCACATTGCTACTGCTGTCTGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCATGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGAGCGGGCTGACGTCCACGGCGGCCGGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGGGGc                                                                                                                                                                    <  2:94375/292‑1 (MQ=255)                                                                                                                     aGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGc                                                                                                                                                                    >  1:380721/1‑300 (MQ=255)                                                                                                                           gggaaaCCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGc                                                                                                                                                                         <  1:242617/286‑1 (MQ=255)                                                                                                                                             gACTGCTGTCGGCAATGATCAGGTCTGTTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGTACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTTGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAGGGCGTGGCTGATCACCAGCAGCAGGCCTGCaa                                                                                                                                            <  1:663836/300‑1 (MQ=255)                                                                                                                                                       ggCAATGATCAGGTCTGGTTGCAGAGAGGCAATGGCTTCCCGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTGGTGCATCGTGATCGTCGGCAATACCGATCGTGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGATAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGc                                                                                                                                                                    <  2:306719/266‑1 (MQ=255)                                                                                                                                                             gATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATaaa                                                                                                                            >  2:267773/1‑300 (MQ=255)                                                                                                                                                             gATCAGGCCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCAAAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGTGTGGCTGCTCACCAGCAGCAGGCATGCAAAAAAAAAACGGAt                                                                                                                               >  2:267781/1‑297 (MQ=255)                                                                                                                                                                      tGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACa                                                                                                                    <  1:90787/299‑1 (MQ=255)                                                                                                                                                                       ggTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGAAGGACTGCCACGGTTTCAGGTGTGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCACTACCAATCGGGCTGCCGTCCACGGCGGCCAGCGCATCGGCGAACGGAAGTTCCAGCACCACAATCCGTTGTGACGTTTTTTCGAGCGTAAACGTACCGCGTTCATCCCGAACCACGGCGGCAAAGGCGTGACTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACAt                                                                                                                   >  2:276734/1‑299 (MQ=255)                                                                                                                                                                                   gCAATGGCTTCCAGGCTCGGCTGCGCGCGCATGCCGACGGACTGCCACGTTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTcc                                                                                                        <  2:178278/298‑1 (MQ=255)                                                                                                                                                                                          cTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCAAATTCCAGCCaaaa                                                                                                >  2:93448/1‑299 (MQ=255)                                                                                                                                                                                           ttCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTAGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAACCACATTCCAGCTAAAAGc                                                                                              >  2:193824/1‑300 (MQ=255)                                                                                                                                                                                                 gCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCa                                                                                        >  1:43489/1‑300 (MQ=255)                                                                                                                                                                                                   tCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCATGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTTTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCaa                                                                                       <  2:58607/299‑1 (MQ=255)                                                                                                                                                                                                               gcgTTCCTACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAac                                                                          <  2:675473/300‑1 (MQ=255)                                                                                                                                                                                                                                 aCGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACg                                                         <  1:188000/299‑1 (MQ=255)                                                                                                                                                                                                                                          ggTGCACACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATTCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCat                                               <  2:94305/300‑1 (MQ=255)                                                                                                                                                                                                                                               gcacgcacTTCGTGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGATTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGNGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCNTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATAc                                           <  2:532486/299‑1 (MQ=255)                                                                                                                                                                                                                                               gcacgcacTTCGGGCAGGATGTGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATAc                                           <  2:485060/299‑1 (MQ=255)                                                                                                                                                                                                                                               gcacgcacTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGNGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTTTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATAc                                           <  1:152471/299‑1 (MQ=255)                                                                                                                                                                                                                                               gcacgcacTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGNGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATAc                                           <  1:504827/299‑1 (MQ=255)                                                                                                                                                                                                                                               gcacgcacTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATAc                                           <  1:203153/299‑1 (MQ=255)                                                                                                                                                                                                                                               gcacgcacTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTGCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATAc                                           <  1:244099/299‑1 (MQ=255)                                                                                                                                                                                                                                               gcacacacTTCGGGCAAGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATAc                                           <  1:244230/299‑1 (MQ=255)                                                                                                                                                                                                                                                 acgcacTTCGTGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAg                                         <  1:29736/299‑1 (MQ=255)                                                                                                                                                                                                                                                 acgcacTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAg                                         <  1:61220/299‑1 (MQ=255)                                                                                                                                                                                                                                                 acacacTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAg                                         <  1:440822/296‑1 (MQ=255)                                                                                                                                                                                                                                                   gcacTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCg                                       <  1:193824/299‑1 (MQ=255)                                                                                                                                                                                                                                                      cTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTCACACATCAGAACTTCAACGACCCCTGCATATACAGCGTgc                                    >  1:282055/1‑299 (MQ=255)                                                                                                                                                                                                                                                      cTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTgc                                    >  1:283871/1‑299 (MQ=255)                                                                                                                                                                                                                                                      cTTCGGGCAGGATGCGNTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACNTCAGAACTTCAACGACCCNTGCATATACAGCGTgc                                    >  1:578626/1‑299 (MQ=255)                                                                                                                                                                                                                                                          gggCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGc                               <  1:639192/300‑1 (MQ=255)                                                                                                                                                                                                                                                               ggATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACTGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTTTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGGAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGcc                          <  1:644938/300‑1 (MQ=255)                                                                                                                                                                                                                                                               ggATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTNCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCAGGCAAGCCGGGCGTTAACACATCAGAACTTCAAAGACCCCTGCATATACAGCGTGCGCGGCTGGcc                          >  2:511115/1‑300 (MQ=255)                                                                                                                                                                                                                                                                     gTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATa                    >  1:409563/1‑300 (MQ=255)                                                                                                                                                                                                                                                                      ttttGCATCGTTATCGTCGGCAATACCGATCGGGCTTACGTCCACTGCGGCCAGCGCATCGGCGAACGATAATTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCATCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAg                   <  2:319578/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                  aTCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTttgttg       <  1:642943/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                       cgGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTACTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGAACACTGCATATACAGCGTGCGCGGCTGGCCTGAAGAGATGCATTTGTTGTTgtc  >  2:449177/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                           TGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGATGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTC  >  NC_000913/4513844‑4514421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑