Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,512,293	T→C	H41R (CAC→CGC)	fecD ←	ferric citrate ABC transporter membrane subunit FecD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,512,293	0	T	C	100.0%	159.1 / NA	47	H41R (CAC→CGC)	fecD	ferric citrate ABC transporter membrane subunit FecD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/29);  total (18/29)

CAGCAATATCAACCCCGCCATGCCGCCCGCAAAGGCCAGCAGCGGCAGCACCATCACGGGCAGTGACGGCATAAGAAGTAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGC  >  NC_000913/4512037‑4512583                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                   cAGCAATATCAACCCCGCCATGCCGCCCGCAAAGGCCAGCAGCGGCAGCACCATCACGGGCAGTGACGGCATAAGAAGTAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCcacggcac                                                                                                                                                                                                                                                            >  1:32466/1‑297 (MQ=255)   gCAATATCAACCCCGCCATGCCGCCCGCAAAGGCCAGCAGCGGCAGCACCATCACGGGCAGTGACGGCATAAGAAGTAGAGCCCCCACAGATGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGTTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCCGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGa                                                                                                                                                                                                                                                       <  2:474742/300‑1 (MQ=255)      atatCAACCCCGCCATGCCGCCCGCAAAGGCCAGCAGCGGCAGCACCATCACGGGCAGTGACGGCATAAGAAGTAGAGCCCACACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGCTGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATc                                                                                                                                                                                                                                                     >  2:674333/1‑299 (MQ=255)               ccGCCATGACGCCCGCAAAGGCCAGCAGCGGCAGCACCATCACGGGCAGTGACGGCATAAGAAGTAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAACACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCAAGGCACGGGGATCACTCCCata                                                                                                                                                                                                                                            >  2:648844/1‑299 (MQ=255)                 gCCATGCCGCCCGCAAAGGCCAGCAGCGGCAGCACCATCACGGGCAGTGACGGCATAAGAAGTAGAGCCCCCACAGAGNCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGAGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATgg                                                                                                                                                                                                                                         <  1:494662/300‑1 (MQ=255)                 gCCATGCCGCCCGCAAAGGCCAGCAGCGGCAGCACCATCACGGGCAGTGACGGCATAAGAAGTAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCatat                                                                                                                                                                                                                                           <  1:226735/298‑1 (MQ=255)                 gCCATGCCGCCCGCAAAGGCCAGCAGCGGCAGCACCATCACGGGCAGTGACGGCATAAGAAGTAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCatat                                                                                                                                                                                                                                           <  2:188393/298‑1 (MQ=255)                 gCCATGCCGCCCGCAAAGGCCAGCAGCGGCAGCACCATCACGGGCAGTGACGGCATAAGAAGTAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATgg                                                                                                                                                                                                                                         <  1:113902/300‑1 (MQ=255)                 gCCATGCCGCCCGCAAAGGCCACCAGCGGCAGCACCATCACGGGCATTGACGGCATAATAAGTAGAGCCCCCACAGAGTCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGTTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCatat                                                                                                                                                                                                                                           <  2:186657/298‑1 (MQ=255)                                      agcagcGGCAGCACCNTCACGGGCAGTGACGGCANAAGAAGTAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGNGATAACAGCGCACAGc                                                                                                                                                                                                                      <  1:583291/298‑1 (MQ=255)                                       gcagcGGCAGCACCATCACGGGCAGTGACGGCATAAGAAGTAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGccc                                                                                                                                                                                                                    >  1:113682/1‑299 (MQ=255)                                            ggCAGCACCATCACTGGCAGTGACGGCATAAGAAGTAGAGCCCCCACAGAGGCCATGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAg                                                                                                                                                                                                               <  2:415320/299‑1 (MQ=255)                                                   ccATCACGGGCAGTGATGGCATAAGAAGTAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCAGTCCCATAGGGAGTGATAACAGCGcatggacc                                                                                                                                                                                                                    >  1:241259/1‑281 (MQ=255)                                                     aTCACGGGCAGTGACGGCATAAGAAGTATAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGTTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAggg                                                                                                                                                                                                       <  2:485534/298‑1 (MQ=255)                                                                                    cccccACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTc                                                                                                                                                                       >  1:476756/1‑299 (MQ=255)                                                                                                   ggCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCatat                                                                                                                                                                                                                                           >  1:329083/1‑216 (MQ=255)                                                                                                   ggCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCatat                                                                                                                                                                                                                                           <  2:329083/216‑1 (MQ=255)                                                                                                               ggTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCaa                                                                                                                                             >  1:63024/1‑298 (MQ=255)                                                                                                                gTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACANAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAggg                                                                                                                                          <  2:228357/300‑1 (MQ=255)                                                                                                                         gagaATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATc                                                                                                                                 <  1:248622/300‑1 (MQ=255)                                                                                                                               atCCGTTGATGCCAGAGGGTTGCGCACAAGCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCGCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGcc                                                                                                                           <  2:378352/300‑1 (MQ=255)                                                                                                                               atCCGGGGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACGCCAGCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATTGAGTGATAACAACGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGcc                                                                                                                           <  2:376997/300‑1 (MQ=255)                                                                                                                               acccGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGcc                                                                                                                           <  2:381175/298‑1 (MQ=255)                                                                                                                                    ttGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCAc                                                                                                                      <  1:61106/299‑1 (MQ=255)                                                                                                                                    gTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCAc                                                                                                                      <  1:62943/300‑1 (MQ=255)                                                                                                                                      gATGCCATAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGATTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAACGCCAGCAc                                                                                                                      <  2:440671/298‑1 (MQ=255)                                                                                                                                            agagGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAGAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTg                                                                                                              <  2:140162/300‑1 (MQ=255)                                                                                                                                            agagGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGcc                                                                                                                <  1:671208/298‑1 (MQ=255)                                                                                                                                                                 tGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGGACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCgggg                                                                                             <  2:268977/296‑1 (MQ=255)                                                                                                                                                                  gTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGc                                                                                        >  1:117254/1‑300 (MQ=255)                                                                                                                                                                          aCGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATAAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAgtgcg                                                                                 <  1:73301/299‑1 (MQ=255)                                                                                                                                                                                gccACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTATTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCTTGGAAGGCCAGTGCGCGTg                                                                             <  2:113682/297‑1 (MQ=255)                                                                                                                                                                                gccACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGGGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTg                                                                             <  1:450296/297‑1 (MQ=255)                                                                                                                                                                                     ggCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACa                                                                     >  1:395931/1‑300 (MQ=255)                                                                                                                                                                                                        aaCAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCg                                                  <  1:523316/300‑1 (MQ=255)                                                                                                                                                                                                            gTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGc                                              >  1:14323/1‑300 (MQ=255)                                                                                                                                                                                                                   cagcaAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTACTCACAAGCCAGACAACGCAAGGGATGCCAATCAGCGCCAGCACTGCGCATGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCcagc                                       >  2:102627/1‑300 (MQ=255)                                                                                                                                                                                                                                  tCGGTACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAgc                                                                                                                                                                                                                             <  2:268622/103‑1 (MQ=255)                                                                                                                                                                                                                                  tCGGTACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAgc                                                                                                                                                                                                                             >  1:268622/1‑103 (MQ=255)                                                                                                                                                                                                                                     gTACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATTGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAg                      <  2:481716/299‑1 (MQ=255)                                                                                                                                                                                                                                     gTACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAACGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCATGCGGGCAGATCTACGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGt                     >  1:38852/1‑300 (MQ=255)                                                                                                                                                                                                                                               aaTACATAATCATGCTCGCGTCCGGCCTGCCAGGCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACCCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTgcgc            <  2:44059/299‑1 (MQ=255)                                                                                                                                                                                                                                                aTACATAATAATGCTCNCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGNCCCCAGCAGCATGCTCACNGGCAGTACGTTGCGCt           >  1:580941/1‑299 (MQ=255)                                                                                                                                                                                                                                                  aCATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCANGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTACGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGAt        >  2:223858/1‑300 (MQ=255)                                                                                                                                                                                                                                                  aCATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTCCCACCCTCGCCTCCTCACAATCCAGACCAAGCAAAGGCTACCAATCCGCGCCAGCACTGCGCCCGCGGACAGATCCCAGGGGAAGGCCAGTGCGCGTGCGAGTATATCTGCACGCAGAATCAGTGTGGCCCCCATCATCATGCTCACTGGCAGTACGTTGCGCTGa         >  2:235340/1‑299 (MQ=255)                                                                                                                                                                                                                                                     taataaTGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCACGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGa     >  2:606694/1‑300 (MQ=255)                                                                                                                                                                                                                                                        taatGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGNTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCNGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGc  <  2:521991/300‑1 (MQ=255)                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                   CAGCAATATCAACCCCGCCATGCCGCCCGCAAAGGCCAGCAGCGGCAGCACCATCACGGGCAGTGACGGCATAAGAAGTAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGC  >  NC_000913/4512037‑4512583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑