Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,336	A→G	E201E (GAA→GAG)	thrC →	threonine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,336	0	A	G	100.0%	278.7 / NA	83	E201E (GAA→GAG)	thrC	threonine synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (42/41);  total (42/41)

GCACAAATGCTGACCCATATTGCGGGTGATAAGCCAGTGACCATTCTGACCGCGACCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTG‑TTGCGCAGCTGCCGCAGGA‑GACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCC  >  NC_000913/4076‑4627                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                      gCACAAATGCTGACCCATATTGCGGGCGATAAGCCAGTGACCATTCTGACCGCGACCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTAAGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTACACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGGTTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGAtt                                                                                                                                                                                                                                                                >  2:14229/1‑300 (MQ=255)       aTGCTGACCCATATTGCGGGCGATAAGCCAGTGACCATTCTGACCGCGACCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTTTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGCCGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATc                                                                                                                                                                                                                                                          >  2:289134/1‑300 (MQ=255)       aTGCTAACCCATATTGCGGGCGATAAGCCAGTGACCATTCTGACCGCGACCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATc                                                                                                                                                                                                                                                          >  2:622427/1‑300 (MQ=255)            gACCCATATTGCGGGCGATAAGCCAGTGACCATTCTGACCGCGACCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGcc                                                                                                                                                                                                                                                      >  1:416731/1‑299 (MQ=255)              cccATATTGCGGGCGATAAGCCAGTGACCATTCTGACCGCGACCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCNGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGtt                                                                                                                                                                                                                                                   >  2:538053/1‑300 (MQ=255)                           cgATAAGCCAGTGACCATTCTGACCGCGACCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGCCGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGAt                                                                                                                                                                                                                                      >  2:293552/2‑300 (MQ=255)                           cgATAAGCCAGTGACCATTCTGACCGCGACCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGAt                                                                                                                                                                                                                                      >  1:468779/2‑300 (MQ=255)                               aaGCCAGTTACCATTCTGACCCCGACCTCCGTTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGGGTACCGAATGGGAAAGGGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGTTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTTGATTAACATCAGCCGTTTGCTGGCGCAGATTTGc                                                                                                                                                                                                                                  <  2:264292/300‑1 (MQ=255)                                    aatGACTATTCTGACCGCTACCTCCTGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGctac                                                                                                                                                                                                                               <  2:114990/296‑1 (MQ=255)                                     gTGACCATTCTGACCGCGACCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGAGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCtactac                                                                                                                                                                                                                            <  1:456029/300‑1 (MQ=255)                                            ttCTGACCGCGACCTCCGTTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAGACTGTTCTTTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAg                                                                                                                                                                                                                     <  2:447559/300‑1 (MQ=255)                                            ttCTGACCGCGACCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAg                                                                                                                                                                                                                     <  1:350621/300‑1 (MQ=255)                                             tCTGACCGCGACCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGc                                                                                                                                                                                                                    <  1:116487/300‑1 (MQ=255)                                                 aCCGCGACCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑tt                                                                                                                                                                                                               >  1:625017/1‑300 (MQ=255)                                                      gACCTCCGGTGATACCGGAGCGGCATTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCNACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTgcgc                                                                                                                                                                                                           <  1:508233/299‑1 (MQ=255)                                                       aCCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAg                                                                                                                                                                                                         <  2:443288/300‑1 (MQ=255)                                                       aCCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTAAGGTTTACCGAATGTGAAAGTGGTTATCCTATATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCa                                                                                                                                                                                                          >  1:68121/1‑299 (MQ=255)                                                              gTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCAATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGc                                                                                                                                                                                                  >  2:421961/1‑300 (MQ=255)                                                                      ggAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGgaag                                                                                                                                                                                            <  1:665769/298‑3 (MQ=255)                                                                                 ggCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTANCATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCt                                                                                                                                                                              >  1:515210/1‑300 (MQ=255)                                                                                 ggCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGTTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCt                                                                                                                                                                              >  2:259344/1‑300 (MQ=255)                                                                                 ggCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTAAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCt                                                                                                                                                                              >  2:259994/1‑300 (MQ=255)                                                                                   cTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCt                                                                                                                                                                              >  2:461123/1‑298 (MQ=255)                                                                                          tttCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCG‑AGGA‑AGCGCGCAACCAGCTGGTTGtctc                                                                                                                                                                     <  1:597809/299‑1 (MQ=255)                                                                                          tttCTACGGTTTACCGAAGGTGAAAGTGGTTGTCCTCTATCCACTAGGCAAAATTAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCTGCAATATTTAAACTGTTTCCAGCGACTGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGGTTGAGAGCTGAAAGTGGCGCTGGGGTTGAACTCAGCTAACTCGAGTAACATCAGTCGGTTGCTGGCGCAGGGTTGCTGCTACTTTGGAGCAG‑TTGTGCAGCTGCCTAAGGA‑AGCGCGCAACCAGCTGGTTGtctc                                                                                                                                                                     <  1:597800/300‑1 (MQ=255)                                                                                           ttCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGTAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGAAGGCGATTTCGATGCATGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGAAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGtct                                                                                                                                                                      >  1:101006/1‑298 (MQ=255)                                                                                           ttCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATTATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCCGCAGTTTGCTGGCGCAGATTTGCTACTAATTTGAAGCAG‑TTGCGCAGCTGCCGCAGGAAGA‑GCGCAAACAGCTAGTTGtct                                                                                                                                                                      >  2:295349/1‑298 (MQ=255)                                                                                           ttCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGtct                                                                                                                                                                      >  2:294825/1‑298 (MQ=255)                                                                                           ttCTAAGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAAACAGCTGGTTGtctc                                                                                                                                                                     >  2:391039/1‑299 (MQ=255)                                                                                                 ggTTTACCGAATGTGAAAGTGGTTCTCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAACCTCAGCTAACTCGATTAACATCAGCAGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTTTCGGTGcaa                                                                                                                                                              >  2:455543/1‑298 (MQ=255)                                                                                                 ggTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATAAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGcaa                                                                                                                                                              >  2:455524/1‑298 (MQ=255)                                                                                                          gaTGTAAAAGTGGTTATCCTTTATCCACGATGCAAAGTCATTCCACTGCAAGAAAAACTGCTCCTTACACTGTGCTGCAAGACCGAAGCTGTTGCCGTCGACGGCGATGTCGAGGCCTGTCAGGCGCTGGGGGAGTAGGCGGTTGATGATGAAGAGCTGAAGGGGGCGCTGGGGGGAAACGCAGCTAACTCGATTAACATTAGCCGTTTGCTTGCGCAGATTTGCCACTGCTTT‑TGGTTGGTTGCGCGGTTGTCGTAGGA‑GGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAAc                                                                                                                                                     <  2:670827/299‑1 (MQ=255)                                                                                                               gAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACtt                                                                                                                                                   >  2:242690/1‑297 (MQ=255)                                                                                                                     ggTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAAACAGCTGGTTGTCTAGGTGCCAAGCGGAAACTTCGGCGAttt                                                                                                                                          >  2:19968/1‑300 (MQ=255)                                                                                                                      gTTATCCTCTATCCCCGGGGCAACATTAGTCCACTGCAAGAAAACCTGTTTTGTACATTGGACGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGCAGCAGGCGTTTGATGCTGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAATTCGATTACCATCAGCAGTTTGCTGGCGCAGAGTTGCTACTACTTTGAAGCAG‑CTGCGCAGCGGCCGAAGGA‑GACGTGCAACCAACTGGTTGTATAGGTGTCAAGCGGAGACTTCggtgcttt                                                                                                                                          >  2:94352/1‑293 (MQ=255)                                                                                                                      gTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGACAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTAATACTTTGAAGCAG‑TTGCGCAGCTACCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTAGGTGCCAAGCGGAAAATTAGGCGAt                                                                                                                                            >  2:92380/1‑297 (MQ=255)                                                                                                                      gTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGAttt                                                                                                                                          >  2:32978/1‑299 (MQ=255)                                                                                                                           ccTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCTCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGAcggc                                                                                                                                    >  1:681120/1‑300 (MQ=255)                                                                                                                           ccTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGAcggc                                                                                                                                    >  2:461663/1‑300 (MQ=255)                                                                                                                             tctATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGAcggc                                                                                                                                    >  1:471974/1‑298 (MQ=255)                                                                                                                             tctATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTAGGTGCCAAGCGGAAACTTCGGCGATTTGAcggc                                                                                                                                    >  1:104481/1‑298 (MQ=255)                                                                                                                                             aaTCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTc                                                                                                                  <  1:383970/300‑1 (MQ=255)                                                                                                                                                 aGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACt                                                                                                               <  1:35720/299‑1 (MQ=255)                                                                                                                                                       cTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGCCGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCAGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTAGGCGATTTGACGGTGGGTCTGCTGGCGAAGTCAACCGGTCt                                                                                                         >  1:215488/1‑299 (MQ=255)                                                                                                                                                           aagagaaatttttcttgacatttgtcggcAATATCTAAAATGTTTCCATCGACAGCAATTGCGATGCCTCTCAGGCGCTGGTGAAGCAGGGGTTTGATGATGAAGAGCTGAAATTGGCGCTGTGGTTAAACTCAGCTAACTCGCTTAACATCAGCCGTTTGCTGNCGCAGATTTGCTACTACTTTGAAGTAG‑TTGCGNAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCgg                                                                                                    <  2:526695/275‑1 (MQ=255)                                                                                                                                                                     gTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTAGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTATGCCGGTGAAGCGttt                                                                                          >  2:186046/1‑300 (MQ=255)                                                                                                                                                                       tCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTACCGGTGAAACGTTTTa                                                                                        >  2:121433/1‑300 (MQ=255)                                                                                                                                                                       tCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGAAGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTa                                                                                        >  2:40176/1‑300 (MQ=255)                                                                                                                                                                       tCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTAGATGCCCAGCGGACACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTATGCCGGTGAAACGTTTTa                                                                                        >  2:122144/1‑300 (MQ=255)                                                                                                                                                                          gTACATTGGTCGGCAATATCGAAACTTTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGTTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATtg                                                                                     <  1:661200/300‑1 (MQ=255)                                                                                                                                                                          gTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATtg                                                                                     <  1:661157/300‑1 (MQ=255)                                                                                                                                                                          gTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTAGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTATGCCGGTGAAACGTTTTAtt                                                                                      >  2:66350/1‑299 (MQ=255)                                                                                                                                                                            aCATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATtgct                                                                                   >  1:52609/1‑300 (MQ=255)                                                                                                                                                                            aCATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCATGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTNGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAATCGGTCTGCCGGTGAAACGTTTTATtgct                                                                                   >  2:542898/1‑300 (MQ=255)                                                                                                                                                                                    cggcAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTAGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTAGGTCTGCCGGTGAAACGTTTTATTGTTGCGACCaa                                                                           >  2:572/1‑300 (MQ=255)                                                                                                                                                                                            tCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACg                                                                   <  1:468140/300‑1 (MQ=255)                                                                                                                                                                                                aaCTGTTGCCATCGACTGCGATTTCGATGCCTGTCAGGCGCTGGTTAAACAGTCGTTGGATGATGAAGAGCTGAAATTGGCGCTGGGTTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCGGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGTA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGTCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTAGGTCTGGCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATAc                                                               <  1:256819/300‑1 (MQ=255)                                                                                                                                                                                                aaCTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTTAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATAc                                                               <  1:460048/300‑1 (MQ=255)                                                                                                                                                                                                aaCTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATAc                                                               <  1:448143/300‑1 (MQ=255)                                                                                                                                                                                                 gcTGTTGCTATCGACGGCGATTTCGAGACCTGTCAGGCGCTGGTGAAGCATTCGTTTGATAATGAAGGGCTGAAAGTAGCACTGGGGTTAAACTCAGCGAACTCGATTAACATCAGCCTTTTACTTGCGCAGATTTGCTACTACTTTTAATCAG‑TTGCGCAGCTGCCGCAGTA‑AGCGCACAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACTGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTGTTATTGCTGCGACCAACGTGAACGATAcc                                                              <  2:149916/299‑1 (MQ=255)                                                                                                                                                                                                 aCTGTTGCCATCGACGTCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTTAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGGGAACGATAc                                                               <  2:136409/299‑1 (MQ=255)                                                                                                                                                                                                     ttGCCATCGACGTCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATAAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGc                                                          <  1:386327/300‑1 (MQ=255)                                                                                                                                                                                                     ttGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCGGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTTGCGCTGGGGTTAAACTNAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGGTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGGGAACGATACCGTGc                                                          <  2:541159/300‑1 (MQ=255)                                                                                                                                                                                                          aTCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGt                                                     <  1:22180/300‑1 (MQ=255)                                                                                                                                                                                                             gACGGCGATTTCGATGCCTGTCATGCGCTGTTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGttt                                                   <  2:621481/299‑1 (MQ=255)                                                                                                                                                                                                              aCGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTcc                                                 <  1:61033/300‑1 (MQ=255)                                                                                                                                                                                                              aCGGCGATTTCGATGCCGGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTcc                                                 <  1:245684/300‑1 (MQ=255)                                                                                                                                                                                                                ggCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTGGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTcc                                                 <  1:245093/298‑1 (MQ=255)                                                                                                                                                                                                                 gCGATTTCGATGCCTGTCATGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGTA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTcc                                                 <  1:61216/297‑1 (MQ=255)                                                                                                                                                                                                                   ggtttCGATGCCTGGCAGGCGCTGGTGAAGCAGGCGTTTGGTGATGAAGAGCTGAAGGTGGCGCTGGGGTTAAACTCAGCTAACTCGCTTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGTA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCac                                            <  2:680453/298‑1 (MQ=255)                                                                                                                                                                                                                      ttCGATGCCTGTCATGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATGTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCacgac                                         <  2:625017/300‑1 (MQ=255)                                                                                                                                                                                                                               tGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAATTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGt                                  <  1:691383/298‑1 (MQ=255)                                                                                                                                                                                                                                gTCAGGCGCTGGTGAAGCAGGCGTTTGATGATTAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGt                               <  1:622427/300‑1 (MQ=255)                                                                                                                                                                                                                                gTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGt                               <  1:289134/300‑1 (MQ=255)                                                                                                                                                                                                                                     gcgcTGGTGAGTCAGGCGTTTGATGATGAGGGGCGGAGAGTTGCGTTTGGTTTAAATCCAGCTACCTCTATTAACAGCAGCCGTTTGCTGGCACAGATTTGCTACTCCTTGTAAGCAG‑TTTCACAACTGCCGCAGGA‑AACGCACAGCCGGCTGGTTGTCTCGGTGCTAAGCGGAAACGTCGGCAATTGGACGGCGGGTCGGCTGGCGAAGTCACTCGGTCTGCCGTTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGGCAGGGGTCAcc                           <  2:365985/299‑1 (MQ=255)                                                                                                                                                                                                                                        cTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCaaa                       >  1:35060/1‑300 (MQ=255)                                                                                                                                                                                                                                          ggTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGc                     >  1:218746/1‑300 (MQ=255)                                                                                                                                                                                                                                          ggTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGATGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTAGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCCCGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGc                     >  1:218803/1‑300 (MQ=255)                                                                                                                                                                                                                                           gTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGTA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCg                    <  1:668766/300‑1 (MQ=255)                                                                                                                                                                                                                                           gTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCg                    <  1:421961/300‑1 (MQ=255)                                                                                                                                                                                                                                               agcaggtttttGATGATGAAGAGCTGAAATTGGCGCTTGGGTTAAACTCAGCTACCTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTTAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTTTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTTCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTc                <  2:438589/292‑1 (MQ=255)                                                                                                                                                                                                                                                   ggCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGc            >  1:92945/1‑300 (MQ=255)                                                                                                                                                                                                                                                             gatgaAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAG‑TTGCGCAGCTGCCGCAGGA‑AGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATcc  <  1:538053/300‑1 (MQ=255)                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                      GCACAAATGCTGACCCATATTGCGGGTGATAAGCCAGTGACCATTCTGACCGCGACCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTG‑TTGCGCAGCTGCCGCAGGA‑GACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCC  >  NC_000913/4076‑4627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑