Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,463,952	G→A	S253F (TCC→TTC)	treC ←	trehalose‑6‑phosphate hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,463,952	0	G	A	98.7%	243.1 / ‑4.5	75	S253F (TCC→TTC)	treC	trehalose‑6‑phosphate hydrolase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (32/42);  total (33/42)
Fisher's exact test for biased strand distribution p-value = 4.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

GCACCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCA‑GGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGCTGGAT‑TCTCCCA‑GTTGAGATCCGCCTGTTC‑‑TGGTGCAAAGAGATGCAAATAGTACTGTTC  >  NC_000913/4463659‑4464241                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                        gcaCCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CAttt                                                                                                                                                                                                                                                                                                  >  2:576143/1‑300 (MQ=255)      aTCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCTGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCTGGATAATCGACCTTCAGTTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCAcc                                                                                                                                                                                                                                                                                              <  2:61584/299‑1 (MQ=255)            aGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCTAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCTGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTa                                                                                                                                                                                                                                                                                            <  2:363715/295‑1 (MQ=255)                                   gTATTCACCTTCATCACCAAAGCTAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCTGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGTAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAacac                                                                                                                                                                                                                                                                <  2:381685/300‑1 (MQ=255)                                   gTATTCACCTTCATCACCAAAGCTAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAacac                                                                                                                                                                                                                                                                <  2:152608/300‑1 (MQ=255)                                   gTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCTGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAacac                                                                                                                                                                                                                                                                <  1:355214/300‑1 (MQ=255)                                   gTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGTTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAacac                                                                                                                                                                                                                                                                <  1:354705/300‑1 (MQ=255)                                   gTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAacac                                                                                                                                                                                                                                                                >  2:688119/1‑300 (MQ=255)                                   gTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAacac                                                                                                                                                                                                                                                                <  1:355237/300‑1 (MQ=255)                                   gTATTCACCTTCAGCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGTAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACAGTCATTAACCCGCGTGGCGTAAacac                                                                                                                                                                                                                                                                <  2:23180/300‑1 (MQ=255)                                    tgttCACCTTCATCACCAAAGCGAGAAACAAGGCGTGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGGTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGTTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAacac                                                                                                                                                                                                                                                                <  2:23915/297‑1 (MQ=255)                                         aCCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATcgc                                                                                                                                                                                                                                                           <  1:76455/299‑1 (MQ=255)                                            ttCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCAACACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCAGCGTGGCGTAAACACATCGCGGtt                                                                                                                                                                                                                                                       >  2:314572/1‑300 (MQ=255)                                            ttCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCAAAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGNCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGtt                                                                                                                                                                                                                                                       >  2:539727/1‑300 (MQ=255)                                                  aCCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCTGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCAtct                                                                                                                                                                                                                                                  <  2:467854/299‑1 (MQ=255)                                                  aCCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGTTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCAtctc                                                                                                                                                                                                                                                 <  2:119234/300‑1 (MQ=255)                                                  aCCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCAtctc                                                                                                                                                                                                                                                 <  1:491276/300‑1 (MQ=255)                                                               aCAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGNCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTAGTGCAAAAACTc                                                                                                                                                                                                                                     >  1:150775/1‑299 (MQ=255)                                                                             aTCGTGGTTACACCAGAACAATGCGTTCCATGCTACGTTGTGTATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGcc                                                                                                                                                                                                                       <  2:53484/299‑1 (MQ=255)                                                                                 tGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTAGTGCAAAAACTCGTGTGCTCGTGGCCCGTc                                                                                                                                                                                                                   >  1:241400/1‑299 (MQ=255)                                                                                  ggTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCgg                                                                                                                                                                                                                 <  1:98172/300‑1 (MQ=255)                                                                                       cacCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTa                                                                                                                                                                                                             >  1:76380/1‑299 (MQ=255)                                                                                        acCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCTGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAg                                                                                                                                                                                                            <  2:445545/299‑1 (MQ=255)                                                                                           aGAACAATGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCTGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCAGTGTAGAAg                                                                                                                                                                                                         <  2:331365/299‑1 (MQ=255)                                                                                           aGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAg                                                                                                                                                                                                         <  2:129376/299‑1 (MQ=255)                                                                                            gAACCAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGAAAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTAGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGc                                                                                                                                                                                                        >  1:129283/1‑299 (MQ=255)                                                                                            gAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACAGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCCGAGCCGCGTATCGCTGGCAATGCTCCAGACTGGTGGAGAA‑CATTTCAACTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTAGTGCAAAAACTAGTGTGCTCGTGGcca                                                                                                                                                                                                                      >  2:677123/1‑284 (MQ=255)                                                                                            gAACAAGGCATTCCATGCTACGTTGTGCATTCATTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGCCCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCACTGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGc                                                                                                                                                                                                        >  1:683447/1‑299 (MQ=255)                                                                                             aaCAAGGCACTCCATGCTACGTTGTGCATTCCTTGTTGCCAGCGGCGGAACAATGTCTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGTTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATCAACCCGCGTGGCGTAAACACATCGCGGTTCACCTCGTGCAAAAACTCGTGTGCTCGTGGCTCGTCGGTGTAGAAg                                                                                                                                                                                                         <  2:345947/297‑1 (MQ=255)                                                                                                     aTTCCATGTTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCGGCGTCCATTTTTCACCACCGGGATCATCGACCTTCAGGTGATGAAAATTCAAGGTCATAGACAATTCACTACCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACATACGGCCATTAACCCGCTTGGCGTAAACACCTCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCAGTAGGTGTAGAAGCGACGCCCGTc                                                                                                                                                                                              >  1:562114/1‑300 (MQ=255)                                                                                                          aTGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGt                                                                                                                                                                                         >  1:114993/1‑300 (MQ=255)                                                                                                            gCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCTGGATAATCGACCTTCAGTTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTcc                                                                                                                                                                                       <  2:302076/300‑1 (MQ=255)                                                                                                                 gTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTAGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTc                                                                                                                                                                                  >  1:348674/1‑300 (MQ=255)                                                                                                                 gTTGTGCAGTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAATTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGTTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTc                                                                                                                                                                                  <  2:418638/300‑1 (MQ=255)                                                                                                                                      gtggagggaCAATGTTTTCAAAGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAGATTAAAGGTCATCGAAAATTCACTGCCTGTAAGAGCAACGTCTCGCTGGCAATCCTCAAAGCTGATGGAGAA‑CATTTCACCTAAGGCCATTAACCCGCGTGGAGTAAACACATCGCGTTCCATCTAGTGCACAAACTCGCGTGCTTGTGGCCCTTCGGTGTAAAAGCGACACCCGCCGCCCTCCCGATCCCCAGGGAAACGCTGGTCttt                                                                                                                                                             >  2:513/9‑300 (MQ=255)                                                                                                                                      gTGGCGGAACAATGTTTTCAACGCAACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTGAAGGTCATCGATAATTCACTGCCTGTCAGAGCCGCGTATCGATGGCCATGCTCAAGGCTAGTGGAGAC‑CATTTCACCTAAGGTCATTAACCAGCGTGGCGTAAACACATTGCGGTTCATCTCGTGCAAAAACTCGTGTGCTc                                                                                                                                                                                                                             >  2:338/1‑236 (MQ=255)                                                                                                                                        ggCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCTGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTgg                                                                                                                                                           <  2:576843/300‑1 (MQ=255)                                                                                                                                        ggCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGTAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTgg                                                                                                                                                           <  1:440431/300‑1 (MQ=255)                                                                                                                                            gAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCCATGCTCAAGGCCGGTGGAGAA‑AATTTAACCTACGGTAATTAACCCGCGTGGCGTAAACACATAGCGGTCCATCTCGTGCAAAAACTCGTGTGCTCGTGGACCGTCGGTGTAGCAGCGACGCCCGTCGCCATCCAGGCCTTCAGGGAAACTCGAGTCTGTGGAGAt                                                                                                                                                       >  2:449013/1‑300 (MQ=255)                                                                                                                                                   gTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTTATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTc                                                                                                                                                >  1:639573/1‑300 (MQ=255)                                                                                                                                                            gCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTTGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATc                                                                                                                                        <  1:681083/299‑1 (MQ=255)                                                                                                                                                            gCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCTTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATc                                                                                                                                        <  2:279435/299‑1 (MQ=255)                                                                                                                                                            gCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATc                                                                                                                                        <  1:670806/299‑1 (MQ=255)                                                                                                                                                               acaAAGTCAGGTTTAGCCAGCATCCATTTTTCACCACCGGGATAATCGACCTTCAGTTGAGGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGGGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCATGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCA‑gg                                                                                                                                   <  1:174385/300‑1 (MQ=255)                                                                                                                                                                  aaGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCTGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGNGTAAACACATCGCGGTTCATCTCGTGCAAAAANTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCA‑Ggcgc                                                                                                                                <  2:524299/300‑1 (MQ=255)                                                                                                                                                                  aaGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAC‑CCTTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTAGTGCAAAAACTCGTGTGCTCGTGGCACGTCGGTGTAGAAGCGACGCCCGTCGACATCCAGGTCTTCAGGGACACGCGGGTCTTTAGAGCTCCGCTTCACcacacctagtc                                                                                                                                   >  2:162534/1‑291 (MQ=255)                                                                                                                                                                  aaGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCA‑Ggcgc                                                                                                                                <  1:602348/300‑1 (MQ=255)                                                                                                                                                                  aaGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATATCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCA‑Ggcgc                                                                                                                                <  1:602426/300‑1 (MQ=255)                                                                                                                                                                    gTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCA‑GGCGCaa                                                                                                                              >  1:67198/1‑300 (MQ=255)                                                                                                                                                                        ggTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCA‑GGCGCAAccc                                                                                                                           >  1:521001/1‑299 (MQ=255)                                                                                                                                                                        ggTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCAGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCA‑GGCGCAAccc                                                                                                                           >  1:520494/1‑299 (MQ=255)                                                                                                                                                                                     tCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCA‑GGCGCAACCCGTCGACCCCACGAt                                                                                                             <  1:443096/300‑1 (MQ=255)                                                                                                                                                                                     tCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCA‑GGCGCAACCCGTCGACCCCACGAt                                                                                                             <  2:442015/300‑1 (MQ=255)                                                                                                                                                                                                ccaccGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTACCCCGCGTGGCGTAACCACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAAAGCGGGTCCTTGGAGATCAGATTCCCCACATCCA‑GGCGCAAACCGTCGACCCAACGATCGGCCCAGAAc                                                                                                  >  2:402384/1‑300 (MQ=255)                                                                                                                                                                                                  acctggATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCA‑GGCGCAACCCGTCGACCCCACGATCGGCCCAGAAc                                                                                                  <  2:458216/294‑1 (MQ=255)                                                                                                                                                                                                  accGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCA‑GGCGCAACCCGTCGACCCCACGATCGGCCCAGAAc                                                                                                  <  1:34929/298‑1 (MQ=255)                                                                                                                                                                                                               aCCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGTTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCA‑GGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACTTTTTTc                                                                                   >  1:112674/1‑300 (MQ=255)                                                                                                                                                                                                                              aaaTTAAAGGTCATAGACGATTCACTGCCTGTCGGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGGAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGGAACGCGGGTCTTTTGAGATCAGATTCACCACATCCA‑GGCGCAACCCTTCGACCCCACGATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTAcc                                                                    <  2:425228/300‑1 (MQ=255)                                                                                                                                                                                                                                     aGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGTAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCA‑GGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGCTGGAt                                                             <  1:620684/300‑1 (MQ=255)                                                                                                                                                                                                                                      ggTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTAGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCA‑GGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGCTGGAt‑t                                                           >  1:529693/1‑300 (MQ=255)                                                                                                                                                                                                                                                aaTTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCA‑GGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGCTGGAT‑TCTCCCA‑GTTg                                                <  1:427608/300‑1 (MQ=255)                                                                                                                                                                                                                                                   tCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCTAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCA‑GGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGCTGGAT‑TCTCCCA‑GTTgaga                                             <  2:478653/300‑1 (MQ=255)                                                                                                                                                                                                                                                           tGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCA‑GGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGCTGGAT‑TCTCCCA‑GTTGAGATCCGCCt                                      >  1:454088/1‑299 (MQ=255)                                                                                                                                                                                                                                                                  gCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCAGTCCAGGTCTTCAGGGAAACNCGGGTCTTTGGAGATCAGATTCACCACATCCA‑GGCGCAACCCGTCGCCCCCACGATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGCTGGAT‑TCTCCCA‑GTTGAGATCCGCCTGTTC‑‑TGGt                            >  2:215915/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  gCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCAACTACGGTCATTAACCCGTGTGGCGTAAACACATCGCGGTTCATCTAGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACNCGGGTCTTTGGAGATCAGATTCACCACATCCA‑GGCGCAACCCGTCGACCCCACGATCGGCCCGGAACTCACAGACTTTTTTCAGCTCTGCGCGTAACGCTGGAT‑TCTCCAA‑GGTGAGATCCGCATGTTC‑‑TGGt                            >  2:216111/1‑300 (MQ=255)                                                                                                                                                                                                                                                                     gcgTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCAAATCCA‑GGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGCTGGAT‑TCTACCA‑GTTGAGATCCGCCTGTTC‑‑TGGTGCa                         >  2:306472/1‑300 (MQ=255)                                                                                                                                                                                                                                                                     gcgTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCAACACATCCA‑GGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGCTGGAT‑TCTCCCA‑GTTGAGATCCGCCTGTTC‑‑TGGTGCa                         >  2:314677/1‑300 (MQ=255)                                                                                                                                                                                                                                                                       gTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGNGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCNTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCA‑GGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGCTGGAT‑TCTCCAA‑GTTGAGATCCGCCTGTTC‑‑TGGTGCaaa                       >  2:536827/1‑300 (MQ=255)                                                                                                                                                                                                                                                                       gTATCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGNGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCNTCGCCGTCCAGGTATTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCA‑GGCGCAACCCGTAGACCCCACGATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGCTGGAT‑TCTCACA‑GTTGAGATCCGCCTGTTC‑‑TGGTGCaaa                       >  2:536836/1‑300 (MQ=255)                                                                                                                                                                                                                                                                         aTCGCTGGCAATGCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCA‑GGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGCTGGAT‑TCTCCCA‑GTTGAGATCCGCCTGTTC‑‑TGGTGCAAaga                     <  1:438603/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                     gCTCAAGGCTGGTGGANGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCACGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGCTGGATGCCTCCCACNTTGAGATCCGCCTGTTCCNTGGTGCAAaga                     >  1:579975/1‑294 (MQ=255)                                                                                                                                                                                                                                                                                     gCTCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATATCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTNTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCA‑GGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGCTGGAT‑TCTCCCA‑GTTGAGATCCGCCTGTTC‑‑TGGTGCAAAGAGATGCAAATAGt         >  2:511051/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                       tCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGTAGATCAGATTCACCACATCCA‑GGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGCTGGAT‑TCTCCCA‑GTTGAGATCCGCCTGTTC‑‑TGGTGCAAAGAGATGCAAATAGTAc       <  2:620860/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                       tCAAGGCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCA‑GGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGCTGGAT‑TCTCCCA‑GTTGAGATCCGCCTGTTC‑‑TGGTGCAAAGAGATGCAAATAGTAc       <  1:102712/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                            gCTGGTGGAGAA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCNTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCA‑GGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGCTGGAT‑TCTCCCA‑GTTGAGATCCGCCTGTTC‑‑TGGTGCAAATAGATGCAAATAGTACTGTTc  <  1:138703/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                        GCACCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGA‑CATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCA‑GGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGCTGGAT‑TCTCCCA‑GTTGAGATCCGCCTGTTC‑‑TGGTGCAAAGAGATGCAAATAGTACTGTTC  >  NC_000913/4463659‑4464241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑