Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,430,584	C→T	P241S (CCA→TCA)	cycA →	D‑serine/alanine/glycine/:H(+)symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,430,584	0	C	T	100.0%	263.1 / NA	79	P241S (CCA→TCA)	cycA	D‑serine/alanine/glycine/:H(+)symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/45);  total (34/45)

GATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGTTCGGTGAGATGGAGTTCTGGTTTGCGATGATCAAAATCGTCGCCATTGTGTCGCTGATTGTCGTCGGCCTGGTCATGGTGGCGATGCACTTTCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGGAGAAAAGC‑CCGTTTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTCTTCCGCTAACAGCGGCGTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCAC  >  NC_000913/4430289‑4430857                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                     gATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGTTCGGTGAGATGGAGTTCTGGTTTGCGATGATCAAAATCGTCGCCATTGTGTCGCTGATTGTCGTCGGCCTGGTCATGGTGGCGATGCACTTTCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCaga                                                                                                                                                                                                                                                                                  >  1:685011/1‑300 (MQ=255)                  cGCTCAATCTCGCCACCGTGAAAATGTTCGGTGAGATGGAGTCCTGGTTTGCGATGATCAAAATCGTCGCCATTGTGTCGCTGATTGTCGTCGGCCTGGTCATGGTGGCGATGCACTTTCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCAGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCAcgc                                                                                                                                                                                                                                                                  >  1:573719/1‑299 (MQ=255)                                      aaaaTGTTCGGTGAGATGGAGTTCTGGTTTGCGATGATCAAAATCGTCGCCATTGTGTCGCTGATTGTCGTCGGCCTGGTCATGGTGGCGATGCACTTTCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATc                                                                                                                                                                                                                                             <  1:680762/300‑1 (MQ=255)                                                   agaTGGAGTTCTGGTTTGAGGTGATCAAAATCGTCGCCATTGTGTCGCTGATTGTCGTCGTCCTGGTCATGGTGGCGATGCACTTTCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCTAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCATAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGt                                                                                                                                                                                                                                <  2:105488/300‑1 (MQ=255)                                                     attgAGTTCTGGTTTTCGGTGATCAAAATCGTCGCCATTGTGTCGCTGATTGTCGTCGGCCTGGTCATGTTGGCGAGGCACTTTCAGTCACCGACTGGTGTGTAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTc                                                                                                                                                                                                                              <  2:294156/297‑1 (MQ=255)                                                     aTGGAGTTCTGGTTTTCGATGATCAAAATCGTCGCCATTGTGTCGCTGATTTTCGTCGGCCTGGTCATGTTGGCGATGCACTTTCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCATAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTc                                                                                                                                                                                                                              <  1:535345/300‑1 (MQ=255)                                                     aTGGAGTTCTGGTTTGCGATGATCAAAATCGTCGCCATTGTGTCGCTGATTGTCGTCGGCCTGGTCATGGTGGCGATGCACTTTCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTc                                                                                                                                                                                                                              <  1:361184/300‑1 (MQ=255)                                                        gAGTTCTGGTTTGCGATGATCAAAATCGTCGCCATTGTGTCGCTGATTGTCGTCGGCCTGGTCATGGTGGCGATGCACTTTCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTAc                                                                                                                                                                                                                           <  1:122819/300‑1 (MQ=255)                                                        gAGTTCTGGTTTGCGATGATCAAAATCGTCGCCATTGTGTCGCGGATTGTCGTCGGCCTGGTCATGGTGGCGATGCACTTTCAGTCACCGACTGGTGTGGAAGCGTCATTCGCACATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTAc                                                                                                                                                                                                                           <  1:121386/300‑1 (MQ=255)                                                        gAGTTCTGGTTTGCGATGATCAAAATCGTCGCCATTGTGTAGCTGATTGTCGTCGGCCTGGTCCTGGTGGCGCTGCACTTTCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGATGGTTCCAGAAAGGTTTAAGTGGCTTCTTTGCAGGATTCCAGATAGCAGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAAACAAAGATTCAGAGAAATCACTGCCACGAGCGATTAACTACATTACGATCCGTAt                                                                                                                                                                                                                                        >  1:191210/1‑287 (MQ=255)                                                          gTTCTGGTTTGCGATGATCAAAATCGTCGCCATTGTGTCGCTGATTGTCGTCGGCCTGGTCATGGTGGCGATGCACTTTCAGTCAACGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGAGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCNGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAGACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCAGTATGTTCTACGt                                                                                                                                                                                                                         >  2:233017/1‑300 (MQ=255)                                                          gTTCTGGTGTGCGATGATCAAAATCGTCGCCATTGTGTCGCTGATTGTCGTCGGCCTGGGCATGGTGGCGATGCACTTTCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGt                                                                                                                                                                                                                         <  2:282879/300‑1 (MQ=255)                                                           ttCTGTTTTGAGATGATCAAAATCGTCGCCATTGTGTCTCTGATTGTCGTCGGCCTGGTCATGGTGGCGATGCACTTTCAGTCACCGACTGGTGTGTAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCTAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTc                                                                                                                                                                                                                        <  2:414189/300‑1 (MQ=255)                                                           ttCTGGTTTGCGATGAGCAAAATCGTCGCCAGTGTGTCTCTGATTGTCGTCGGCCTGGTCATGGTGGCGATGCACTTTCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTc                                                                                                                                                                                                                        <  2:414185/300‑1 (MQ=255)                                                               gttttgtggtgatCAAAATCTTCGCAATTGTTTCACTGATTGTCGTCGGCCTGGTCCTGGTGGAGATGCCCTTTCATTCCCCGACTGGTTTGGAAGCGTCCTTCGCGCATTTTTGAAATGACGGCGGCTGGTTCCCGAAAGGTTTACGTGGCTTCTTTGCCGGATTCCAAATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAGTCACTGc                                                                                                                                                                                                                                                                       >  2:200837/10‑249 (MQ=255)                                                               ggTTTGCGATGATCAAAATCGTCGCCATTGTGTCGCTGATTGTCGTCGGCCTGGTCATGGTGGCGATGCACTTTCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTc                                                                                                                                                                                                                     >  2:411895/1‑299 (MQ=255)                                                               ggTTTGCGATGATCAAAATCGTCGCAATTGTGTCGCTGATTGTCGTCGGCCTGGTCATGGTGGCGATGCACTTTCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCACGAAAGGTTTAAGTGGCTTCTTTGCAGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTc                                                                                                                                                                                                                     >  2:260840/1‑299 (MQ=255)                                                                         gatCAAAATCGTCGCCATTGTGTCGCTGATTGTCGTCGGCCTGGTCATGGTGGCGATGCACTTTCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATtgt                                                                                                                                                                                                          >  2:485180/1‑300 (MQ=255)                                                                                   gtcgCCATTGTTTCACTGATTGTCGTCGGCCTGGTCATGGTGGCGATGCACTTTCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTAtatc                                                                                                                                                                                                 <  1:686231/299‑4 (MQ=255)                                                                                   gtcgCCATTGTGTCGCTGATTGTCGTCGGCCTGGTCATGGTGGCGATGCACTTTCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTAtatc                                                                                                                                                                                                 <  1:685744/299‑4 (MQ=255)                                                                                   gtcgCCATTGTGTCGCTGATTGTCGTCGGCCTGGTCATGGTGGCGATGCACTTTCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTc                                                                                                                                                                                                 <  1:414940/299‑1 (MQ=255)                                                                                         aTTGTGTCGCTGATTGTCGTCGGCCTGGTCATGGTGGCGATGCACTTTCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGAc                                                                                                                                                                                           >  2:429368/1‑299 (MQ=255)                                                                                                    gATTGTCGTCGGCCTGGTCATGGTGGCGATGCACTTTCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTc                                                                                                                                                                              >  1:23254/1‑300 (MQ=255)                                                                                                       tGTCGTCGGCCTGGTCATGGTTGCGATGCACTTTCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAANGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCTTGACGCCGTGG‑AGTTCG‑Gt                                                                                                                                                                          <  1:135246/300‑1 (MQ=255)                                                                                                         tctttggCCTTGTCGTGATGGCGGTGCACTGTTAGTCACCGACTGGTGTGGAAGCTTCATTCGTGCATTTTTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAGTCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGGGATTATGTCCGTGACACCGTGG‑AGTTCG‑GTAg                                                                                                                                                                        <  2:49650/295‑1 (MQ=255)                                                                                                         tcgtcgGCCTGGTCATGGTGGCGATGCACTTTCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTANGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAg                                                                                                                                                                        <  2:498878/300‑1 (MQ=255)                                                                                                                   ggTCATGGTGGCGATGCACTTTCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTAGGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGGAGaa                                                                                                                                                              <  1:394176/300‑1 (MQ=255)                                                                                                                   ggTCATGGTGGCGATGCACTTTCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGGAGaa                                                                                                                                                              >  1:625319/1‑300 (MQ=255)                                                                                                                   ggTCATGGTGGCGATGCACTTTCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCAGTGG‑AGTTCG‑GTAGTCCAGGAGaa                                                                                                                                                              >  2:237894/1‑300 (MQ=255)                                                                                                                    gTCATGGTTGCGATGCACTTTCAGTCACCGACTGGTGTGGAATCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCTAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGGAGaaa                                                                                                                                                             <  1:454836/300‑1 (MQ=255)                                                                                                                     tCATGTTGTCGATGCACTTTCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCATAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGTAGaaa                                                                                                                                                             <  1:454797/299‑1 (MQ=255)                                                                                                                                    attgtCAGTCACCGGTTGGTGTTTAAGCGTTATTCGCGCATGGGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTTCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCATAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTGCG‑GTAGTCCCGGAGAAAAGC‑CCGTTTGTTGAAc                                                                                                                                            <  2:370831/296‑1 (MQ=255)                                                                                                                                    aCTTTCAGTCACCGACTGGTGTGTAAGCGTCATTCGCGCATTTGGGGAATGACGGCGGCTGGCTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCATAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGGAGAAAAGC‑CCGTTTGTTGAAc                                                                                                                                            <  2:370772/300‑1 (MQ=255)                                                                                                                                    aCTTTCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGTAGAAAAGC‑CCGTTTGTTGAAc                                                                                                                                            <  2:76286/300‑1 (MQ=255)                                                                                                                                    aCTTTCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGGAGAAAAGC‑CCGTTTGTTGAAc                                                                                                                                            <  1:93166/300‑1 (MQ=255)                                                                                                                                      tttCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGGGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGGAGAAAAGC‑CCGTTTGTTGAAc                                                                                                                                            <  2:677552/298‑1 (MQ=255)                                                                                                                                       ttCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGGAGAAAAGC‑CCGTTTGTTGaa                                                                                                                                             >  1:665411/1‑296 (MQ=255)                                                                                                                                       ttCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGACTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGGAGAAAAGC‑CCGTTTGTTGaa                                                                                                                                             >  2:685210/1‑296 (MQ=255)                                                                                                                                           gTCACCGACTGGTGTGNAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGGGAGTTCGNGTAATCCCAGAGAAAAGCNCCGTTTGTTGagcgtgt                                                                                                                                        >  1:575941/1‑294 (MQ=255)                                                                                                                                                    tGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGGAGAAAAGC‑CCGTTTGTTGAACTGTTCGTGTTGGTAg                                                                                                                             <  2:591923/299‑1 (MQ=255)                                                                                                                                                     ggTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCAGGAGAAAAGC‑CTGTTTGTTGAACTGTTCGTGTTGGTa                                                                                                                              >  2:146415/1‑297 (MQ=255)                                                                                                                                                     ggTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCAGGATTCCAGATAGCGGTTTTCGCTTTAGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGCGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCAGGAGAAAAGC‑ACGTTTGTTGAACTGTTCGTGTTGGTa                                                                                                                              >  2:5154/1‑297 (MQ=255)                                                                                                                                                      gtgtGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGGAGAAAAGC‑CCGTTTGTTGAACTGTTCGTGTTGGTAGGGc                                                                                                                          >  1:467061/1‑300 (MQ=255)                                                                                                                                                      gtgtGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGGAGAAAAGC‑CCGTTTGTTGAACTGTTCGTGTTGGTAGGGc                                                                                                                          >  2:646499/1‑300 (MQ=255)                                                                                                                                                      gtgtGGAAGCGTCATTCGCGCATTTGTGGAACGACGGCGGCTGTTTCCCGAAAGGTTTAATTTGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGGAGAAAAGC‑CCGTTTGTTGAACTGTTCGTGTTGGTAGGGc                                                                                                                          <  2:27518/300‑1 (MQ=255)                                                                                                                                                             aGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTANGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGGAGAAAAGC‑CCGTTTGTTGAACTGTTCGTGTTGGTAGGGctgcctgc                                                                                                                   >  1:136173/1‑300 (MQ=255)                                                                                                                                                                          cATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGGAGAAAAGC‑CCGTTTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGt                                                                                                       >  2:662331/1‑299 (MQ=255)                                                                                                                                                                            tttGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGGAGAAAAGC‑CCGTTTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGAt                                                                                                    >  2:483855/1‑300 (MQ=255)                                                                                                                                                                                               tGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCTGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGGAGAAAAGC‑CCGTTTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGAcc                                                                                 <  1:454905/300‑1 (MQ=255)                                                                                                                                                                                                 gTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGGTAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGGAGAAAAGC‑CCGTTTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACctc                                                                               >  1:658571/1‑300 (MQ=255)                                                                                                                                                                                                 gTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGGAGAAAAGC‑CCGTTTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACctc                                                                               >  1:658674/1‑300 (MQ=255)                                                                                                                                                                                                          ctgcgagatgtgtataagagacagATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGGAGAAAAGC‑CCGTTTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTc                                                                      >  1:8232/24‑300 (MQ=255)                                                                                                                                                                                                                       ttcttTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGGAGAAAAGC‑CCGTTTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTCTTCCGCTAACAg                                                          <  1:334631/299‑1 (MQ=255)                                                                                                                                                                                                                                       aGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGTAGAAAAGC‑CCGTTTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTCTTCCGCTAACAGCGGCGTCTTCTCTAc                                           <  1:98116/298‑1 (MQ=255)                                                                                                                                                                                                                                       aGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGGAGAAAAGC‑CCGTTTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTCTTCCGCTAACAGCGGCGTCTTCTCTAc                                           <  1:97545/298‑1 (MQ=255)                                                                                                                                                                                                                                       aGATAGCGGTTTTCGCTTTCGGGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGGAGAAAAGC‑CCGTTTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTCTTCCGCTAACAGCGGCGTCTTCTCTAc                                           <  2:23254/298‑1 (MQ=255)                                                                                                                                                                                                                                               gTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGGAGAAAAGC‑CCGTTTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTCTTCCGCTAACAGCGGCGTCTTCTCTACCAGCCGTAt                                  >  2:579420/1‑299 (MQ=255)                                                                                                                                                                                                                                               gTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGGAGAAAAGC‑CCGTTTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCAGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTCTTCCGCTAACAGCGGCGTCTTCTCTACCAGCCGTAt                                  >  2:239386/1‑299 (MQ=255)                                                                                                                                                                                                                                                ttttCGCTGTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGTAGAAAAGC‑CCGTTTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTCTTCCGCTAACAGCGGCGTCTTCTCTACCAGCCGTATGc                                <  2:108413/300‑1 (MQ=255)                                                                                                                                                                                                                                                tcttCGCTTTCGGTGGTATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATCAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGGAGAAAAGC‑CCGTTTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTCTTCCGCTAACAGCGGTGTCTTCTCTACCAGCCGTATGc                                <  2:491007/298‑1 (MQ=255)                                                                                                                                                                                                                                                gtttCGCTTTCGTGGGGGTTGAGTTGGTAGGTACAACAGTTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCTTGACGCCGTGG‑AGTTCG‑TTAGTCCCGTAGAAAAGC‑CCGTTTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTCTTCCGCTAACAGCGGCGTTTTCTCTACCAGCCGTATGc                                <  2:392809/299‑1 (MQ=255)                                                                                                                                                                                                                                                  ttCGTTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGGAGAAAAGC‑CCGTTTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTCTTCCGCTAACAGCGGCGTCTTCTCTACCAGCCGTATGCTg                              <  2:328062/300‑1 (MQ=255)                                                                                                                                                                                                                                                   tCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCNCGGAGAAAAGC‑CCGTTTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTCTTCCGCTAACAGCGGCGTCTTCTCTACCAGCCGTATGCTGt                             <  2:495948/300‑1 (MQ=255)                                                                                                                                                                                                                                                          cGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGGAGAAAAGC‑CCGTTTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTCTTCCGCTAACAGCGGCGTCTTCTCTACCAGCCGTATGCTGTTTGGTCt                      >  1:175094/1‑300 (MQ=255)                                                                                                                                                                                                                                                           gTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGNATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGGAGAAAAGC‑CCGTTTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTCTTCCGCTAACAGCGGCGTCTTCTCTACCAGCCGTATGCTGTTTGGTCt                      <  2:540049/299‑1 (MQ=255)                                                                                                                                                                                                                                                            tGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCATGGAAATCACTGCCACGCGCGATTAACTCCGTTCCTATCCGNATCATTATGTTCTACGTCTTCGCGCTGATTGGGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGGAGAAAAGC‑CCGCTTGTTGAACTGTTCGGGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTTTGGTGCTGACCTCTGCGGCGTCTTCCGCTAACAGCGGCGTCTTCTCTACCAGCCGTATGCTGTTTGGTCt                      <  2:540048/298‑1 (MQ=255)                                                                                                                                                                                                                                                               ggATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGGAGAAAAGC‑CCGTTTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTCTTCCGCTAACAGCGGCGTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGgcgc                 >  2:669257/1‑300 (MQ=255)                                                                                                                                                                                                                                                                   tGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGGAGAAAAGC‑CCGTTTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTCTTCCGCTAACAGCGGCGTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGa             <  1:411895/300‑1 (MQ=255)                                                                                                                                                                                                                                                                    gAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGTAGAAAAGC‑CCGTTTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTCTTCCGCTAACAGCGGCGTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGaa            <  1:237894/300‑1 (MQ=255)                                                                                                                                                                                                                                                                    gAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGGAGAAAAGC‑CCGTTTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTCTTCCGCTAACAGCGGCGTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGaa            <  1:169108/300‑1 (MQ=255)                                                                                                                                                                                                                                                                    gAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCAGGAGAAAAGC‑CCGTTTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTCTTCCGCTAACAGCGGCGTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGaa            >  1:387645/1‑300 (MQ=255)                                                                                                                                                                                                                                                                     aGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAAGCACTGCCACGCGCGATTAACTCCATGCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCTTGG‑AGTTCG‑GTAGTCCCGGAGAAAAGC‑CCGTTTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTTTGGTGCTTACCTCTGCGGCGTCTTCCGCTAACAGCGGCGTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGaa            <  2:254090/299‑1 (MQ=255)                                                                                                                                                                                                                                                                      gCTGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGGAGAAAAGC‑CCGTTTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTCTTCCGCTAACAGCGGCGTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGAAgg          <  2:428486/300‑1 (MQ=255)                                                                                                                                                                                                                                                                        tGGTAGGTACAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCTTGG‑AGTTCG‑GTAGTCCCGGAGAAAAGC‑CCGTTTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGTGTCTTCCGCTAACAGCGGCGTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGAAGgt         <  2:573719/299‑1 (MQ=255)                                                                                                                                                                                                                                                                         ggtaggtaCAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGGAGAAAAGC‑CCGTTTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCANCTTTGTGGTGCTGACCTCTGCGGCGTCTTCCGCTAACAGCGGCGTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGAAGgtgt       >  2:540702/1‑300 (MQ=255)                                                                                                                                                                                                                                                                            aggtaCAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGGAGAAAAGC‑CCGTTTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTCTTCCGCTAACAGCGGCGTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGc    >  2:575305/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              gtaCAACAGCTGCGGAAACCAAAGATTCAGGGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGGAGAAAAGC‑CCGTTTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTCTTCCGCTAACAGCGGCGTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCAc  <  2:685011/300‑1 (MQ=255)                                                                                                                                                                                                                                                                              gtaCAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGGAGAAAAGC‑CCGTTTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTCTTCCGCTAACAGCGGCGTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCAc  >  1:257075/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              gtaCAACAGCTGCGGAAACCAAAGATTCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGGAGAAAAGC‑CCGTTTGTTGAACTGTTCGTGCTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTCTTCCGCTAACAGCGGCGTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCAc  >  1:257087/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                     GATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGTTCGGTGAGATGGAGTTCTGGTTTGCGATGATCAAAATCGTCGCCATTGTGTCGCTGATTGTCGTCGGCCTGGTCATGGTGGCGATGCACTTTCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTGCCACGCGCGATTAACTCCATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTGATTATGTCCGTGACGCCGTGG‑AGTTCG‑GTAGTCCCGGAGAAAAGC‑CCGTTTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTCTTCCGCTAACAGCGGCGTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCAC  >  NC_000913/4430289‑4430857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑