Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,320,543	G→A	L41F (CTT→TTT)	phnI ←	carbon‑phosphorus lyase core complex subunit PhnI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,320,543	0	G	A	100.0%	181.3 / NA	55	L41F (CTT→TTT)	phnI	carbon‑phosphorus lyase core complex subunit PhnI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/28);  total (27/28)

GTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAGCTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGCGCTCGGTGAGTTCGTGCAGAATGCACTCCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCAC  >  NC_000913/4320290‑4320829                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                               gtgtAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCAGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATc                                                                                                                                                                                                                                                   >  1:316052/1‑299 (MQ=255)     aGTCGTAGGTTGGGCCAAGCAGCTGGCAGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTc                                                                                                                                                                                                                                              >  1:643713/1‑300 (MQ=255)      gTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCGGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCgac                                                                                                                                                                                                                                                >  2:433619/1‑295 (MQ=255)      gTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCt                                                                                                                                                                                                                                               >  2:470669/1‑298 (MQ=255)      gTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCt                                                                                                                                                                                                                                               >  1:653026/1‑298 (MQ=255)      gTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTc                                                                                                                                                                                                                                              >  1:114163/1‑299 (MQ=255)      gTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCAGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCACTCTGGGCGACGCTCAGTTCGGGCAAATCGGTATTGCCt                                                                                                                                                                                                                                               >  2:76307/1‑298 (MQ=255)      gTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCAGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCt                                                                                                                                                                                                                                               >  2:96675/1‑298 (MQ=255)      gTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCAGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCt                                                                                                                                                                                                                                               >  1:21020/1‑298 (MQ=255)      gTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCAGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTc                                                                                                                                                                                                                                              >  1:115057/1‑299 (MQ=255)          tAGGTTGTGCCAAGCAGCTGGCCGCCTGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGt                                                                                                                                                                                                                                         <  2:115057/300‑1 (MQ=255)          tAGGTTGGGCCAATCAGCTGGCCACCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGt                                                                                                                                                                                                                                         <  2:114163/300‑1 (MQ=255)           aGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCAGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGAAGCTCAGTTCGGGCAAATCGGTATCGCCTAGGCGTc                                                                                                                                                                                                                                        >  2:287372/1‑300 (MQ=255)                 ggCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGctct                                                                                                                                                                                                                                  >  2:491645/1‑300 (MQ=255)                 ggCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACAGCCGGCTTCGCAGACGTGATGCGGTAGGCGTGCAGCAGGAAAATCGCTTAGATGTTATCGCCGCTGGCCTGTTTCAGCGCAAGCGCCGCCAGTTCGCGGCCGGCAATGCCGACTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATTTGGGCGACGTTCAGTTCGGGCAAATCGGTATCGCCt                                                                                                                                                                                                                                               >  2:492202/1‑287 (MQ=255)                                 gccgGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTggg                                                                                                                                                                                                                   <  1:645492/299‑1 (MQ=255)                                    gggAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCATGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGggc                                                                                                                                                                                                                  <  2:331218/297‑1 (MQ=255)                                       aaTGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGt                                                                                                                                                                                                             <  2:672132/299‑1 (MQ=255)                                                          gagaTACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCt                                                                                                                                                                                         >  1:566024/1‑300 (MQ=255)                                                          gagaTACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTAGGCGTCGGCTCCCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCt                                                                                                                                                                                         >  1:566019/1‑300 (MQ=255)                                                           agaTACGGCGTTCTAGACGCATCCCGGTGGTGTCGAGTGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGTAATGCCGCCTTCGGTCATCACGCGAGCTACCGCGAGGTTAAACTGCTGTGCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGc                                                                                                                                                                                              <  2:485337/294‑1 (MQ=255)                                                                         agaCGCATCCCGGTGGTGTCGAGCTGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTAc                                                                                                                                                                          <  1:331769/300‑1 (MQ=255)                                                                                    ggtggtGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCATGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTTGGGCAAATCGTTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGTCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGcac                                                                                                                                                                  <  2:349540/297‑1 (MQ=255)                                                                                    ggtggtGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGNGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGcac                                                                                                                                                                  <  2:507166/297‑1 (MQ=255)                                                                                    ggtggtGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGTGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTTGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGcac                                                                                                                                                                  <  2:411479/297‑1 (MQ=255)                                                                                    ggtggtGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGcac                                                                                                                                                                  <  1:162123/297‑1 (MQ=255)                                                                                    ggtggtGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCacac                                                                                                                                                                <  1:84144/299‑1 (MQ=255)                                                                                     gtggtgTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCt                                                                                                                                                              <  1:280714/300‑1 (MQ=255)                                                                                      tggtgTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACTGCAACGTACATCAGCACACCTc                                                                                                                                                             <  1:474613/300‑1 (MQ=255)                                                                                           tCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACAt                                                                                                                                                        <  2:630284/300‑1 (MQ=255)                                                                                             gAGCGGCTCGATTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTTGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATg                                                                                                                                                       <  2:19725/299‑1 (MQ=255)                                                                                             gAGCGGCGCGCTTATCGCCAGCTTCGCCAACGTGATGCGGGAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAGCGTACATCAACACACCTCcacac                                                                                                                                                        <  2:630176/298‑2 (MQ=255)                                                                                                    tCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGtt                                                                                                                                                <  1:434204/299‑1 (MQ=255)                                                                                                                                          gcgcAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGAt                                                                                                          <  1:14789/299‑1 (MQ=255)                                                                                                                                              agcagGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGt                                                                                                     <  1:96675/300‑1 (MQ=255)                                                                                                                                                    aaaaTCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGc                                                                                               <  1:470669/300‑1 (MQ=255)                                                                                                                                                                 gggtATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTAGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCACCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGaa                                                                                     >  2:635168/4‑297 (MQ=255)                                                                                                                                                                               gCCTGTTTCAGCGCCAGCGCCGACAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTAGCCCACTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTAGATGCAGAGCGGGAACGGATGc                                                                              >  2:252411/1‑290 (MQ=255)                                                                                                                                                                                ccTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTAGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGCGCTCGGt                                                                   >  1:442440/1‑300 (MQ=255)                                                                                                                                                                                   gTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGCGCTCGGTGa                                                                 >  2:615679/1‑299 (MQ=255)                                                                                                                                                                                   gTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGCGCTCGGTGa                                                                 >  1:247917/1‑299 (MQ=255)                                                                                                                                                                                   gTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCAAAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGCGCTCGGTGa                                                                 >  1:249707/1‑299 (MQ=255)                                                                                                                                                                                                    gccAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCNACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGCGCTCGGTGAGTTCGTGCAGAATGCAc                                                <  1:139057/299‑1 (MQ=255)                                                                                                                                                                                                    gccAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGCGCTCGGTGAGTTCGTGCAGAATGCACt                                               <  1:628727/300‑1 (MQ=255)                                                                                                                                                                                                        gTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGTGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGCGCTCGGTGAGTTCGTGCAGAATGCACTc                                              <  2:649570/297‑1 (MQ=255)                                                                                                                                                                                                               gTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCTCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGCGCTCGGTGAGTTCGTGCAGAATGCACTCCGGCAGCTGc                                    >  1:436994/1‑300 (MQ=255)                                                                                                                                                                                                                   gCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTTTGCGACGCTCAGTTCGGGCAAATCGGTATCACCTCGGCGTCGGCTCTCTTGCAGTGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGCGCTCGGTGAGTTCGTGCAGAATGCACTCCGGCAGCTGCGGAg                                <  2:191058/300‑1 (MQ=255)                                                                                                                                                                                                                   gCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCGGTGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGCGCTCGTTGAGTTCGTGCAGAATACACTCCGGCAGCTGCGGAg                                <  2:192964/300‑1 (MQ=255)                                                                                                                                                                                                                           gccTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTAGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCACCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGCGCTCGGTGAGTTCGTGCAGAATGCACTCCGGCAGCTGCGGAGCGATCAtt                        >  2:154786/1‑300 (MQ=255)                                                                                                                                                                                                                                cGGTCATCAAGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTAGCCCCCTTTCACGGCAACGTACATCAGCACACCTACACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGCTGCCGAGCGGGAACGGATGCGGGCGCTCGGTGAGTTCGTGCAGAATGCACTCAGGCAGCTGCGGAGCGATCATTCGttc                   >  2:697062/1‑300 (MQ=255)                                                                                                                                                                                                                                 ggTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCNCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGCGCTCGGTGAGTTCGTGCAGAATGCACTCCGGCAGCTGCGGAGCGATCATTCGttc                   <  2:554861/299‑1 (MQ=255)                                                                                                                                                                                                                                             aTCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGCGCTCGGTGAGTTCGTGCAGAATGCACTCCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATAccc      >  1:406935/1‑300 (MQ=255)                                                                                                                                                                                                                                             aTCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTACACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGCGCTCGGTGAGTTCGTGCAGAATGCACTCCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATAccc      >  1:406836/1‑300 (MQ=255)                                                                                                                                                                                                                                             aTCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTAGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGCGCTCGGTGAGTTCGTGCAGAATGCACTCCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATAccc      >  1:296898/1‑300 (MQ=255)                                                                                                                                                                                                                                                 aCCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGCGCTCGGTGAGTTCGTGCAGAATGCACTCCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCAc  <  1:409981/300‑1 (MQ=255)                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                               GTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAGCTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGCGCTCGGTGAGTTCGTGCAGAATGCACTCCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCAC  >  NC_000913/4320290‑4320829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑