Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,320,416	G→A	T83I (ACC→ATC)	phnI ←	carbon‑phosphorus lyase core complex subunit PhnI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,320,416	0	G	A	100.0%	144.1 / NA	45	T83I (ACC→ATC)	phnI	carbon‑phosphorus lyase core complex subunit PhnI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/24);  total (21/24)

GTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAGCTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTT  >  NC_000913/4320122‑4320687                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                gTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCg                                                                                                                                                                                                                                                                             <  2:634112/299‑1 (MQ=255)     gggTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTa                                                                                                                                                                                                                                                                          >  1:19725/1‑298 (MQ=255)               aTCCGGCTGTGCGCAGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAAGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCAGTTTGCCAGCAGGGTAAAATCGAGTAGGTGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCAAGCTTCGCCAACTTGATGCGGTAGGCGCgcagca                                                                                                                                                                                                                                                               >  2:139057/1‑299 (MQ=255)                            ccgcTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCa                                                                                                                                                                                                                                                 >  1:611659/1‑300 (MQ=255)                            ccgcTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCa                                                                                                                                                                                                                                                 >  2:409981/1‑300 (MQ=255)                                                 gCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGc                                                                                                                                                                                                                                >  1:554861/1‑296 (MQ=255)                                                    aGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGttt                                                                                                                                                                                                                          <  1:352063/299‑1 (MQ=255)                                                          tGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTcagcg                                                                                                                                                                                                                     <  1:471803/298‑1 (MQ=255)                                                          tGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTcagcg                                                                                                                                                                                                                     <  2:610273/298‑1 (MQ=255)                                                                       ggCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCCCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGtt                                                                                                                                                                                                      <  2:611659/300‑1 (MQ=255)                                                                                 gTGCGGCGACGTCTGCTGTTCGCTGTCTGCGGTGTTCAACGTCGGCGCTTCGCCGTTTGCCAGCAGAGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCTAGACGCATCCCGTTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGc                                                                                                                                                                                            <  2:339227/300‑1 (MQ=255)                                                                                          cGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCTAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCACCGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGCTCGCGGTCGGCAATgccgcc                                                                                                                                                                                   >  2:261488/1‑300 (MQ=255)                                                                                                                                                                         gtgtAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCAGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATc                                                                                                     >  1:316052/1‑299 (MQ=255)                                                                                                                                                                             aGTCGTAGGTTGGGCCAAGCAGCTGGCAGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTc                                                                                                >  1:643713/1‑300 (MQ=255)                                                                                                                                                                              gTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCGGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCgac                                                                                                  >  2:433619/1‑295 (MQ=255)                                                                                                                                                                              gTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCt                                                                                                 >  2:470669/1‑298 (MQ=255)                                                                                                                                                                              gTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCt                                                                                                 >  1:653026/1‑298 (MQ=255)                                                                                                                                                                              gTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTc                                                                                                >  1:114163/1‑299 (MQ=255)                                                                                                                                                                              gTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCAGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCACTCTGGGCGACGCTCAGTTCGGGCAAATCGGTATTGCCt                                                                                                 >  2:76307/1‑298 (MQ=255)                                                                                                                                                                              gTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCAGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCt                                                                                                 >  2:96675/1‑298 (MQ=255)                                                                                                                                                                              gTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCAGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCt                                                                                                 >  1:21020/1‑298 (MQ=255)                                                                                                                                                                              gTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCAGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTc                                                                                                >  1:115057/1‑299 (MQ=255)                                                                                                                                                                                  tAGGTTGTGCCAAGCAGCTGGCCGCCTGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGt                                                                                           <  2:115057/300‑1 (MQ=255)                                                                                                                                                                                  tAGGTTGGGCCAATCAGCTGGCCACCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGt                                                                                           <  2:114163/300‑1 (MQ=255)                                                                                                                                                                                   aGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCAGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGAAGCTCAGTTCGGGCAAATCGGTATCGCCTAGGCGTc                                                                                          >  2:287372/1‑300 (MQ=255)                                                                                                                                                                                         ggCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGctct                                                                                    >  2:491645/1‑300 (MQ=255)                                                                                                                                                                                         ggCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACAGCCGGCTTCGCAGACGTGATGCGGTAGGCGTGCAGCAGGAAAATCGCTTAGATGTTATCGCCGCTGGCCTGTTTCAGCGCAAGCGCCGCCAGTTCGCGGCCGGCAATGCCGACTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATTTGGGCGACGTTCAGTTCGGGCAAATCGGTATCGCCt                                                                                                 >  2:492202/1‑287 (MQ=255)                                                                                                                                                                                                         gccgGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTggg                                                                     <  1:645492/299‑1 (MQ=255)                                                                                                                                                                                                            gggAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCATGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGggc                                                                    <  2:331218/297‑1 (MQ=255)                                                                                                                                                                                                               aaTGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGt                                                               <  2:672132/299‑1 (MQ=255)                                                                                                                                                                                                                                  gagaTACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCt                                           >  1:566024/1‑300 (MQ=255)                                                                                                                                                                                                                                  gagaTACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTAGGCGTCGGCTCCCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCt                                           >  1:566019/1‑300 (MQ=255)                                                                                                                                                                                                                                   agaTACGGCGTTCTAGACGCATCCCGGTGGTGTCGAGTGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGTAATGCCGCCTTCGGTCATCACGCGAGCTACCGCGAGGTTAAACTGCTGTGCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGc                                                <  2:485337/294‑1 (MQ=255)                                                                                                                                                                                                                                                 agaCGCATCCCGGTGGTGTCGAGCTGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTAc                            <  1:331769/300‑1 (MQ=255)                                                                                                                                                                                                                                                            ggtggtGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCATGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTTGGGCAAATCGTTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGTCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGcac                    <  2:349540/297‑1 (MQ=255)                                                                                                                                                                                                                                                            ggtggtGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGNGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGcac                    <  2:507166/297‑1 (MQ=255)                                                                                                                                                                                                                                                            ggtggtGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGTGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTTGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGcac                    <  2:411479/297‑1 (MQ=255)                                                                                                                                                                                                                                                            ggtggtGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGcac                    <  1:162123/297‑1 (MQ=255)                                                                                                                                                                                                                                                            ggtggtGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCacac                  <  1:84144/299‑1 (MQ=255)                                                                                                                                                                                                                                                             gtggtgTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCt                <  1:280714/300‑1 (MQ=255)                                                                                                                                                                                                                                                              tggtgTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACTGCAACGTACATCAGCACACCTc               <  1:474613/300‑1 (MQ=255)                                                                                                                                                                                                                                                                   tCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACAt          <  2:630284/300‑1 (MQ=255)                                                                                                                                                                                                                                                                     gAGCGGCTCGATTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTTGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATg         <  2:19725/299‑1 (MQ=255)                                                                                                                                                                                                                                                                     gAGCGGCGCGCTTATCGCCAGCTTCGCCAACGTGATGCGGGAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAGCGTACATCAACACACCTCcacac          <  2:630176/298‑2 (MQ=255)                                                                                                                                                                                                                                                                            tCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGtt  <  1:434204/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                GTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAGCTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTT  >  NC_000913/4320122‑4320687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑