Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,320,007	G→A	H219H (CAC→CAT)	phnI ←	carbon‑phosphorus lyase core complex subunit PhnI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,320,007	0	G	A	100.0%	156.3 / NA	47	H219H (CAC→CAT)	phnI	carbon‑phosphorus lyase core complex subunit PhnI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/30);  total (17/30)

GCATGTGCCAGCACGAACTCTTCATCCTGCGCCGGGCCTGTCGCGTGCTCGCCGTACTCCGGGGCTTGCAGAGCGCGGTCGACCAGCGCCATCGCCATCGCTTTGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGG  >  NC_000913/4319714‑4320290                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                            gCATGTGCCAGCACGAACTCTTCATCCGGCGCCGGGCCTGTCGCGTGCTCGCCGTACTCCGGGGCTTGCAGAGCGCGGTCGACCAGCGCCATCGCCATCGCTTTGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTg                                                                                                                                                                                                                                                                                       <  1:262538/300‑1 (MQ=255)                     ttCATCCTGCGCCGGGCCTGTCGCGTGCTCGCCGTACTCCGGGGCTTGCAGAGCGCGGTCGACCAGCGCCATCGCCATCGCTTTGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGt                                                                                                                                                                                                                                                                   <  1:456566/300‑1 (MQ=255)                            tgcacttggcttGTCACGTGCTCGCCGTACTCCGGGGCTTGCAGAGCGCGGTCGACCAGCGCCATCGCCATCGCTTTGCACTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATTAGCAGTTCGCCGACGTTCACCGCAAATCCCAACTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGt                                                                                                                                                                                                                                                              <  2:15053/287‑1 (MQ=255)                            tGCGCCGGGCCTGTCGCGTGCTCGCCGTACTCCGGGGCTTGCAGAGCGCGGTCGACCAGCGCCATCGCCATCGCTTTGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGt                                                                                                                                                                                                                                                              <  1:539244/298‑1 (MQ=255)                             gcgcCGGGCCTGTCGCGTGCTCGCCGTACTCCGGTGCTTGCAGAGCGCGGTCGACCAGCGCCATCGCCATCGCTTTGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGt                                                                                                                                                                                                                                                              <  2:271976/297‑1 (MQ=255)                               gcCGGGCCTGTCGCGTGCTCGCCGTGCTCCTGGGCTTGCAGAGCGCGGTCGACCAGCGCCATCGCCATCGCTTTGCGCTCGCTCATGCCGAACACCAGCCTGTAGCCGCGCGTGGAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCACTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCTAGCTCTTCCGGCACAATCGACACGTTGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGgcc                                                                                                                                                                                                                                                         <  2:435417/300‑1 (MQ=255)                               gcCGGGCCTGTCGCGTGCTCGCCGTACTCCTGGGCTTGCAGAGCGCGGTCGACCAGCGCCATCGCCATCGCTTTGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCTGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGgcc                                                                                                                                                                                                                                                         <  2:58715/300‑1 (MQ=255)                               gcCGGGCCTGTCGCGTGCTCGCCGTACTCCGGGGCTTGCAGAGCGCGGTCGACCAGCGCCATCGCCATCGCTTTGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGgcc                                                                                                                                                                                                                                                         <  1:19103/300‑1 (MQ=255)                               gcCGGGCCTGTCGCGTGCTCGCCGTACTCCGGGGCTTGCAGAGCGCGGTCGACCAGCGCCATCGCCATCGCTTTGCGCNCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGgcc                                                                                                                                                                                                                                                         <  1:502972/300‑1 (MQ=255)                                    gCCTGTCGCGTGCTCGCCGTACTCCGGGGCTTGCAGAGCGCGGTCGACCAGCGCCATCGCCATCGCTTTGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGgccagcgc                                                                                                                                                                                                                                                    <  1:13290/300‑1 (MQ=255)                                                      gTACTCCGGGGCTTGCAGAGCGCGGTCGACCAGCGCCATCGCCATCGCGTTGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCATTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACTGATGATGGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTtcgtc                                                                                                                                                                                                                                  <  2:154642/300‑1 (MQ=255)                                                         cTCCGGGGCTTGCAGAGCGCGGTCGACCAGCGCCATCGCCATCGCTTTGCGCCCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTcgcc                                                                                                                                                                                                                               >  1:607904/1‑300 (MQ=255)                                                               ggCTTGCAGAGCGCGGTCGACCAGCGCCATCGCCATCGCTTTGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCAt                                                                                                                                                                                                                         >  1:339227/1‑300 (MQ=255)                                                               ggCTTGCAGAGCGCGGTCGACCAGCGCCATCGCCATCGCTTTGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTAAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCNGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCAt                                                                                                                                                                                                                         >  2:556047/1‑300 (MQ=255)                                                                       gagCGCGGTCGACCAGCGCCATCGCCATCGCTTTGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCATGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAActgct                                                                                                                                                                                                                 >  1:331218/1‑300 (MQ=255)                                                                             ggTCGACCAGCGCCATCGCCATCGCTTTGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAAc                                                                                                                                                                                                           <  1:75049/300‑1 (MQ=255)                                                                                            tcgccatcgcTTTGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGc                                                                                                                                                                                            <  1:566713/300‑1 (MQ=255)                                                                                                      tttGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAAc                                                                                                                                                                                  <  2:338220/300‑1 (MQ=255)                                                                                                      tttGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAAc                                                                                                                                                                                  <  1:334535/300‑1 (MQ=255)                                                                                                       ttGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTAAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAAcc                                                                                                                                                                                 <  1:556047/300‑1 (MQ=255)                                                                                                         gcgcTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAAcc                                                                                                                                                                                 >  2:360632/1‑298 (MQ=255)                                                                                                         gcgcTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAAcc                                                                                                                                                                                 >  1:532279/1‑298 (MQ=255)                                                                                                                   aTGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCACGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCACCTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGc                                                                                                                                                                       >  2:331769/1‑298 (MQ=255)                                                                                                                     gacGAACACCAGCCCGTAGCCGCGCGTGAAGTGTGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGGTCACCGCAAATTCCAGCTCTTCCTGCACAATCGACACGTCGATATGGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGgcc                                                                                                                                                                                                                                                         <  1:212557/212‑1 (MQ=255)                                                                                                                                    gTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGTTGATGTCATCCGGc                                                                                                                                                      <  2:620338/298‑1 (MQ=255)                                                                                                                                                 aagggcggcgACTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCTGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGcgccgc                                                                                                                                            <  2:104205/291‑1 (MQ=255)                                                                                                                                                 aaGTGCGGCGGCTCACCCTGCGGGTCGAGAAAACCTTTAACCATTTCACACTCATTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATcc                                                                                                                                       <  2:576535/300‑1 (MQ=255)                                                                                                                                                 aaGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATcc                                                                                                                                       <  2:576542/300‑1 (MQ=255)                                                                                                                                                   gTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACNGCAAATCCCAGCTCTTCCTGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCctc                                                                                                                                     <  1:503650/300‑1 (MQ=255)                                                                                                                                                   gTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACNGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCctc                                                                                                                                     <  1:503669/300‑1 (MQ=255)                                                                                                                                                          ggcTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAAc                                                                                                                              <  1:457267/300‑1 (MQ=255)                                                                                                                                                                          cGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGAc                                                                                                              >  1:349540/1‑300 (MQ=255)                                                                                                                                                                                    gTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGG‑GGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCcagcag                                                                                                    <  1:627047/299‑1 (MQ=255)                                                                                                                                                                                                     cAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTgcggc                                                                                    >  2:463167/1‑299 (MQ=255)                                                                                                                                                                                                       gTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGAc                                                                                 >  1:411479/1‑300 (MQ=255)                                                                                                                                                                                                       gTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGAc                                                                                 >  2:645492/1‑300 (MQ=255)                                                                                                                                                                                                       gTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCATTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGAc                                                                                 >  1:485337/1‑300 (MQ=255)                                                                                                                                                                                                         catcaGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGAc                                                                                 >  1:672132/1‑298 (MQ=255)                                                                                                                                                                                                                     gCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGc                                                                    <  1:529585/299‑1 (MQ=255)                                                                                                                                                                                                                                               ctTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAGCCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCATGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCtt                                         >  2:352063/1‑300 (MQ=255)                                                                                                                                                                                                                                                ttCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTc                                        >  1:610273/1‑300 (MQ=255)                                                                                                                                                                                                                                                                acGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGANGTCATCCGGCTGTGCGCCGCTATCCTCTTCAANCTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCcagcag                         <  2:532279/299‑1 (MQ=255)                                                                                                                                                                                                                                                                   tCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTa                     >  1:630284/1‑300 (MQ=255)                                                                                                                                                                                                                                                                   tCGATATAGCCGCTGCGGATCTCGCATGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTAGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCAAGCAGGGTa                     >  1:630176/1‑300 (MQ=255)                                                                                                                                                                                                                                                                       tataGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCGGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAAt                 <  2:607904/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                      tctcGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAACTAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGTCAGCAGGGTAAAATCGAGCAGGCGATggg  <  2:599214/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                      tctcGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATggg  <  1:360632/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                            GCATGTGCCAGCACGAACTCTTCATCCTGCGCCGGGCCTGTCGCGTGCTCGCCGTACTCCGGGGCTTGCAGAGCGCGGTCGACCAGCGCCATCGCCATCGCTTTGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGG  >  NC_000913/4319714‑4320290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑