Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,306,035	C→T	G188D (GGC→GAC)	yjcS ←	linear primary‑alkylsulfatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,306,035	0	C	T	100.0%	279.6 / NA	83	G188D (GGC→GAC)	yjcS	linear primary‑alkylsulfatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (42/41);  total (42/41)

AGGAAGTCAAACTCCAGGCCGTCGATAATCATCTTCTCGCCAGTTCTGACGATAGTTTTCGTCGGTGCAATAATGCTCGGGTCGCCCGTTGCCAGCGTCACGCCAAGGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGCCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCAACGAACGTAATGTTAGAGATATCCTGGCCGCGCACCTGATACATTTTATCGGTGACTTTGAACAGGCCAGAAATACCGTTGATTTGCGACTGACGCCACAGGCTGGGGTTTACGGTTTCCGGCGCTGCGGCATTAATATCAAAT  >  NC_000913/4305744‑4306325                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                    aGGAAGTCAAACTCCAGGCCGTCGATAATCATCTTCTCGCCAGTTCTGACGATAGTTTTCGTCGGTGCAATAATGCTCGGGTCGCCCGTTGCCAGCGTCACGCCAAGGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAg                                                                                                                                                                                                                                                                                             <  2:560505/300‑1 (MQ=255) aGGAAGTCAAACTCCAGGCCGTCGATAATCATCTTCTCGCCAGTTCTGACGATAGTTTTCGTCGGTGCAATAATGCTCGGGTCGCCCGTTGCCAGCGTCACGCCAAGGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAg                                                                                                                                                                                                                                                                                             <  1:48586/300‑1 (MQ=255)    aaGTCAAACTCCAGGCCGTCGATAATCATCTTCTCGCCAGTTCTGACGATAGTTTTCGTCGGTGCAATAATGCTCGGGTCGCCCGTTGCCAGCGTCACGCCAAGGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTgg                                                                                                                                                                                                                                                                                          <  1:600129/300‑1 (MQ=255)      gTCAAACTCCAGGCCGTCGATAATCACCTTCTCGCCAGTTCTGACGATAGTTTTCGTCGGTGCAATAATGCTCGGGTCGCCCGTTGCCAGCGTCACGCCAAGGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTc                                                                                                                                                                                                                                                                                        <  2:168918/300‑1 (MQ=255)         aaaCTCCAGGCCGTCGATAATCATCTTCTCGCCAGTTCTGACGATAGTTTTCGTCGGTGCAATAATGCTCGGGTCGCCCGTTGCCAGCGTCACGCCAAGGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGgt                                                                                                                                                                                                                                                                                     <  2:654188/300‑1 (MQ=255)           aCTCCAGGCCGTCGATAATCATCTTCTCGCCAGTTCTGACGATAGTTTTCGTCGGTGCAATAATGCTCGGGTCGCCCGTTGCCAGCGTCACGCCAAGGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGgtgt                                                                                                                                                                                                                                                                                   <  2:693692/300‑1 (MQ=255)           aCTCCAGGCCGTCGATAATCATCTTCTCGCCAGTTCTGACGATAGTTTTCGTCGGTGCAATAATGCTCGGGTCGCCCGTTGCCAGCGTCACGCCAAGGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGgtgt                                                                                                                                                                                                                                                                                   >  1:524500/1‑300 (MQ=255)                atgCCGTCGATAATCATCTTCTCGCCAGTTCTGACGATAGTTTTCGTCGGTGCAATAATGCTCGGGTCGCCCGTTGCCAGCGTCACGCCAAGGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCtg                                                                                                                                                                                                                                                                              <  2:568586/298‑1 (MQ=255)                aGGCCGTCGATAATCGTCTTCTCGCCAGTGCTGACGATAGTTTTCGTCGGTGCAATAATGCTCTGGTCGCCCGTTGCCAGCGTCACGCCAAGGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCTGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCtg                                                                                                                                                                                                                                                                              <  2:127155/300‑1 (MQ=255)                aGGCCGTCGATAATCATNTTCTCGCCAGTTCTGACGNTAGTTTTCGTCGGTGCAATAATGCTCGGGTCGCCCGTTGCCAGCGTCACGCCAAGGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATNGTGGTCGGTGTGGCtg                                                                                                                                                                                                                                                                              <  1:581342/300‑1 (MQ=255)                aGGCCGTCGATAATCATCTTCTCGCCAGTTCTGACGATAGTTTTCGTCGGTGCAATAATGCTCGGGTCGCCCGTTGNCAGCGTCACGCCAAGGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGc                                                                                                                                                                                                                                                                                <  1:500665/298‑1 (MQ=255)                     gTCGATAATCATCTTCTCGCCAGTTCTGACGATAGTTTTCGTCGGTGCAATAATGCTCGGGTCGCCCGTTGCCAGCGTCACGCCAAGGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAgt                                                                                                                                                                                                                                                                         <  1:663907/300‑1 (MQ=255)                          tAATCATCTTCTCGCCAGTTCTGACGATAGTTTTCGTCGGTGCAATAATGCTCGGGTCGCCCGTTGCCAGCGTCACGCCAAGGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGa                                                                                                                                                                                                                                                                    <  1:660677/300‑1 (MQ=255)                            atcatcTTCTCGCCAGTTCTGACGATAGTTTTCGTCGGTGCAATAATGCTCGGGTCGCCCGTTGCCAGCGTCACGCCAAGGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATa                                                                                                                                                                                                                                                                  <  2:588931/300‑1 (MQ=255)                               atcTTCTCGCCGGTTCTGACGATAGTTTTCGTCGGTGCAATAATGCTCGGGTCGCCCGTTGCCAGCGTCACGCCAAGGCCATTACCTACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGATTAGAGCGCACTGCGGCTCATGATGTTACCCGCCANCACGTTTTCGCTGATGGCTTCGTCCATAAAGCNTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCATAGATAATGCCTTTCACGTCACCGTAGTGGTCGGTGTGGCTGTGAGTGTAGATAAc                                                                                                                                                                                                                                                                <  2:527305/299‑1 (MQ=255)                               atcTTCTCGCCAGTCCTGACGATATTTTTCGTCGGTGCAATAATGCTCGGGTCGCCCGTTGCCAGCGTCACGCCAAGGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCANCACGTTTTCGCTGATGGCGTCGTCCATAAAGCNTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCATAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGGGGCTGTGAGTGTAGATAAc                                                                                                                                                                                                                                                                <  2:527306/299‑1 (MQ=255)                                tcttctCGCCAGTTCTGACGATAGTTTTCGTCGGTGCAATAATGCTCGGGTCGCCCGTTGCCAGCGTCACGCCAAGGCCATTACCCACATNGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACgg                                                                                                                                                                                                                                                              <  2:554703/300‑1 (MQ=255)                                                gACGATAGTTTTCGTCGGTGCAATAATGCTCGGGTCGCCCGTTGCCAGCGTCACGCCAAGGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTg                                                                                                                                                                                                                                              <  1:485896/300‑1 (MQ=255)                                                                 gTGCAATAATGCTCGGGTCGCCCGTTGCCAGCGTCACGCCAAGGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGTGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCAAGTCAACAGAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGACAACAACCGGTTTTTGCGGACGATGc                                                                                                                                                                                                                                    >  2:162272/1‑293 (MQ=255)                                                                 gTGCAATAATGCTCGGGTCGCCCGTTGCCAGCGTCACGCCAAGGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGt                                                                                                                                                                                                                             >  1:216293/1‑300 (MQ=255)                                                                      ataatGCTCGGGTCGCCCGTTGCCAGCGTCACGCCAAGGCCATTACCCACATTGCCTTGCGCGTTGTGTGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCATCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATCATGCCTTTAACGTCAACATAGTGGTCGGTGTGGCTGTGAGTGTAGATACAGGCAACAATCGGTTTTTGATGATGATGCTGGAAGTa                                                                                                                                                                                                                            >  2:17426/1‑296 (MQ=255)                                                                      ataatGCTCGGGTCGCCCGTTGCCAGCGTCACGCCAAGGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCAACATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTaaa                                                                                                                                                                                                                          >  2:14996/1‑298 (MQ=255)                                                                      ataatGCTCGGGTCGCCCGTTGCCAGCGTCACGCCAAGGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCAGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCAACATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAAAAATCGGTTTTTGc                                                                                                                                                                                                                                             >  2:15819/1‑279 (MQ=255)                                                                             tcttgTCGCCCGTTGCCATCGTCACGCCAAGGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTTATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGTCTTCAGAGATAATGCCTTTCACGTCACCATATTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTg                                                                                                                                                                                                                <  2:91589/296‑1 (MQ=255)                                                                                    gCCCGTTGCCGGCTTCACGCCAAGGCCATTACCCACATTTCCTGGTGCGTTGTTCTGCAGTAACAGACCGTAAGATTAGAGCGCACGGCGTCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTTCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATGGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACGGTTGGTTTTTGCGGATGATGCTGGAAGTAAAGG‑TCAAGTGCGGCtt                                                                                                                                                                                                          <  2:327364/299‑1 (MQ=255)                                                                                               gCGTCACGCCAAGGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCCGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAggc                                                                                                                                                                                               >  1:550652/1‑299 (MQ=255)                                                                                               gCGTCACGCCAAGGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAggc                                                                                                                                                                                               >  1:550568/1‑299 (MQ=255)                                                                                                cGTCACGCCAAGGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAggc                                                                                                                                                                                               >  1:399477/1‑298 (MQ=255)                                                                                                     cGCCAAGGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCa                                                                                                                                                                                        >  1:598118/1‑300 (MQ=255)                                                                                                     cGCCAAGGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGAGAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGGCTCCAGTGCGGCTTTTGCGGCAGGCGGCGTc                                                                                                                                                                                         >  2:576019/1‑300 (MQ=255)                                                                                                     cGCCAAGGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGAGAACGGCAACAATCGGTTTTTGCGGACGAGGCTGGAAGTAAAGGCGCAAGTGCGGCTTTTGCGGCAGGCGGCGTc                                                                                                                                                                                         >  2:576027/1‑300 (MQ=255)                                                                                                          aGGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCATGGGCAATCACCTGAACTTTGCCGGATTGAACATCGGCTTCATAGATAATGCCTTTCACTTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCtt                                                                                                                                                                                                          <  2:327216/277‑1 (MQ=255)                                                                                                           ggCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGc                                                                                                                                                                                   >  2:671812/1‑299 (MQ=255)                                                                                                             ccATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGNCTTCGTCCATAAAGCCTGCAGGGGCAATCACCNGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGc                                                                                                                                                                                   >  2:519058/1‑297 (MQ=255)                                                                                                               aTTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCAGGATTTAACATCGGCTTCAGAGATAATGCATTTCACGTCACCATAGTGGTCGGTGTGGATGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTc                                                                                                                                                                              >  2:74296/1‑300 (MQ=255)                                                                                                                      acaTTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACa                                                                                                                                                                       >  1:86326/1‑300 (MQ=255)                                                                                                                           gCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTNTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACaataat                                                                                                                                                                  >  1:496349/1‑300 (MQ=255)                                                                                                                             cTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCAGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGc                                                                                                                                                                >  1:4108/1‑300 (MQ=255)                                                                                                                              ttGCGCGTTGTGCGGCAGTAACAGACCTTAAGAGTAGAGCGCACTGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCNCGTCACCATAGTGGTCGGTGTGGCTGTGAGTGNAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGc                                                                                                                                                                <  2:524500/299‑1 (MQ=255)                                                                                                                              ttGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGc                                                                                                                                                                <  1:538267/299‑1 (MQ=255)                                                                                                                               tgctctttGTGCGACAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTCCGTCCATAAATCCTGCAGGGGCAATCACCTGAACTTTGCCGGAGTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGGGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTGGCGGCAGGCGGCGTCACCAGCGGGGCGATAACAATAATGCCt                                                                                                                                                              <  2:46750/294‑1 (MQ=255)                                                                                                                                       gtgCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGcc                                                                                                                                                      >  1:282076/1‑300 (MQ=255)                                                                                                                                       gtgCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGNGGTCGGTGTGGCTGTGAGTGTCGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCCTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGcc                                                                                                                                                      >  2:230672/1‑300 (MQ=255)                                                                                                                                        tgCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGccc                                                                                                                                                     <  1:491275/300‑1 (MQ=255)                                                                                                                                             cAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCaac                                                                                                                                                >  1:108090/1‑300 (MQ=255)                                                                                                                                               gTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCaacga                                                                                                                                              >  1:556763/1‑300 (MQ=255)                                                                                                                                                     gACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCAACGAACGTaa                                                                                                                                        >  1:425265/1‑300 (MQ=255)                                                                                                                                                     gACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGACGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTAAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCAACAGCGGGTCGATAACAATAATGCCTTTCTAGCCCTCAACGAACGTaa                                                                                                                                        >  2:695805/1‑300 (MQ=255)                                                                                                                                                        cGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGTACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGGTAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGNGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATCACAATAATACCTTTCTAGCCCTACACGAACGTAATGt                                                                                                                                     >  2:221482/1‑300 (MQ=255)                                                                                                                                                        cGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGNGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCAACGAACGTAATGt                                                                                                                                     >  2:218071/1‑300 (MQ=255)                                                                                                                                                        cGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCCGAACTTTGAAGGATTTAACATCGGCTTCAGAGATAATGCATTTCACGTCAACATAGTGGTCGGTGTGGCTGTGNGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATCATGCCTTTCTCGCCCTAaacgaat                                                                                                                                            >  2:220897/1‑292 (MQ=255)                                                                                                                                                          tAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGNATTTAACATCGGCTTCAGAGATAATGCCTTTCNCGTCAACATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCAACGAACGTAATGTTa                                                                                                                                   >  2:526292/1‑300 (MQ=255)                                                                                                                                                             gagTAGAGCGCACGGCNGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATNATGCCTTTCTCGCCCTCANCGAACGTAATGTTaga                                                                                                                                 >  1:589412/1‑299 (MQ=255)                                                                                                                                                             gagTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCAACGAACGTAATGTTagag                                                                                                                                <  1:473611/300‑1 (MQ=255)                                                                                                                                                             gagTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTACATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCATTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAGAGG‑TAAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATACTGCCTTTCTCGCACTCAACGAACGTAGTGTTaga                                                                                                                                 >  2:283075/1‑299 (MQ=255)                                                                                                                                                               gTAGAGCGCACTGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCAACGAACGTAATGTTAGAGat                                                                                                                              >  1:602028/1‑300 (MQ=255)                                                                                                                                                                   agCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATNACGGCAACAATCGGTTTTTGCGGACGATGCTGNAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCAACGAACGTAATGTTAGAGATATcc                                                                                                                          <  2:535549/300‑1 (MQ=255)                                                                                                                                                                    gcgcACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCAACGAACGTAATGTTAGAGATATCCt                                                                                                                         >  1:662519/1‑300 (MQ=255)                                                                                                                                                                         cggcggCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCAACGAACGTAATGTTAGAGATATCCTGGcc                                                                                                                     >  2:443103/1‑299 (MQ=255)                                                                                                                                                                               cTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCAACGAACGTAATGTTAGAGATATCCTGGCCGCGCAcc                                                                                                              >  1:549251/1‑300 (MQ=255)                                                                                                                                                                                      tgTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGTCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGGGTGGCTGGAAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCAACGAACGTAATGTTAGAGATATCCTGGCCGCGCACCTGATACa                                                                                                       <  2:66824/300‑1 (MQ=255)                                                                                                                                                                                      tgTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGACGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCAACGAACGTAATGTTAGAGATATCCTGGCCGCGCACCTGATACa                                                                                                       <  1:43540/300‑1 (MQ=255)                                                                                                                                                                                              gCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCATGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCATAGATAATGCCCTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑GCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGTTCGATAACAATAATGCCTTTCTCGCCCTCGACGAACTTAATGTTATAGATATCCTGGCCGCGCACCTGATACATTTTATc                                                                                                <  2:353780/299‑1 (MQ=255)                                                                                                                                                                                                          tCGCTGATTGCTTCGTCCATAAAGCCTGCAGTGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCAACGAACGTAATGTTAGAGATATCCTGGCCGCGCACCTGATACATTTTATCGGTGACTTTGAAc                                                                                   <  2:116817/300‑1 (MQ=255)                                                                                                                                                                                                             cTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCAACATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCAACGAACGTAATGTTAGAGATATCCTGGCCGCGCACCTGATACATTTTATCGGTGACTTTGAACa                                                                                  >  2:423839/1‑298 (MQ=255)                                                                                                                                                                                                                     ttcttCCATAAAGCCTGCAGTTGCAATCACCTGAACTTTGCCTGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCGCGCCCTCAACGAACGTAATGTTAGAGATATCCTGGCCGCGCACCTGATACATTTTATCGGTGACTTTGAACAGGCCAGaaa                                                                         <  2:163969/295‑1 (MQ=255)                                                                                                                                                                                                                     ttCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGTTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCTGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCAACGAACGTAATGTTAGAGATATCCTGGCCGCGCACCTGATACATTTTATCGGTGACTTTGAACAGGCCAGaaa                                                                         <  2:99817/299‑1 (MQ=255)                                                                                                                                                                                                                        gTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGNCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCAACGAACGTAATGTTAGAGATATCCTGGCCGCGCACCTGATACATTTTATCGGTGACTTTGAACAGGCCAGAAATAc                                                                      <  2:496349/299‑1 (MQ=255)                                                                                                                                                                                                                        gTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCAACGAACGTAATGTTAGAGATATCCTGGCCGCGCACCTGATACATTTTATCGGTGACTTTGAACAGGCCAGAAATAcc                                                                     >  1:58028/1‑300 (MQ=255)                                                                                                                                                                                                                         tCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGNACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCAACGAACGTAATGTTAGAGATATCCTGGCCGCGCACCTGATACATTTTATCGGTGACTTTGAACAGGCCAGAAATACCg                                                                    <  2:216293/300‑1 (MQ=255)                                                                                                                                                                                                                                          ggCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCAACGAACGTAATGTTAGAGATATCCTGGCCGCGCACCTGATACATTTTATCGGTGACTTTGAACAGGCCAGAAATACCGTTGATTTGCGACTGAc                                                    >  1:657149/1‑299 (MQ=255)                                                                                                                                                                                                                                                    tGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGTTCGATAACAATAATGCCTTTCTCGCCCTCAACGAACGTAATGTTAGAGATATCCTGGCCGCGCACCTGATACATTTTATCGGTGACTTTGAACAGGCCAGAAATACCGTTGATTTGCGACTGACGCCACAGGCt                                          <  1:359408/299‑1 (MQ=255)                                                                                                                                                                                                                                                       aCTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCAACGAACGTAATGTTAGAGATATCCTGGCCGCGCACCTGATACATTTTATCGGTGACTTTGAACAGGCCAGAAATACCGTTGATTTGCGAATGACGCCAAAGGCt                                          >  2:330868/1‑296 (MQ=255)                                                                                                                                                                                                                                                         tttGCCGGATTTAACATCTGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCAACGAACGTAATGTTAGAGATATCCTGGCCGCGCACCTGATACATTTTATCGGTGACTTTGAACAGGCCAGAAATACCGTTGATTTGCGACTGACGCCACAGGCTGGGGt                                     <  2:562257/299‑1 (MQ=255)                                                                                                                                                                                                                                                         tttGCCGGATTTAACATCGGCTTCATAGATAATGCCTTTCACGTCACCAGAGTGGTCGGTGTGGCTGTGAGTGTAGATAGCGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCATGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTGTCTCGCCCTCAACGAACGTAATGTTAGAGATATCCTGGCCGCGCACCTGATACATTTTATCGGTGACTTTGAACAGGCCAGAAATACCGTTGATTTGCGACTGACGCCACAGGCTGGGGt                                     <  2:562341/299‑1 (MQ=255)                                                                                                                                                                                                                                                         tttGCCGGATTTAACATCGGCGTCAGAAATAATGCCCTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGATGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCATGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCAACGAACGTAATGTTAGAGATATCCTGGCCGCGCACCTGATACATTTTATCGGTGACTTTGAACAGGCCAGAAATACCGTTGATTTGCGACTGACGCCACAGGCt                                          <  2:11564/294‑1 (MQ=255)                                                                                                                                                                                                                                                               ggATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCAACGAACGTAATGTTAGAGATATCCTGGCCGCGCACCTGATACATTTTATCGGTGACTTTGAACAGGCCAGAAATACCGTTGATTTGCGACTGACGCCACAGGCTGGGGTTTACGGt                              >  2:480016/1‑300 (MQ=255)                                                                                                                                                                                                                                                               ggATTTAACATCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCAACGAACGTAATGTTAGAGATATCCTGGACGCGCACCTGATACATTTTATCGGTGACTTTGAACAGGCCAGAAATACCGTTGATTTGCGACTGACGCCACAGGCTGGGGTTTACGGt                              >  2:479994/1‑300 (MQ=255)                                                                                                                                                                                                                                                                        aTCGGCTTCATAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCAACGAACGTAATGTTAGAGATATCCTGGCCGCGCACCTGATACATTTTATCGGTGACTTTGAACAGGCCAGAAATACCGTTGATTTGCGACTGACGCCACAGGCTGGGGTTTACGGTTTCCGgcgc                     <  2:20701/300‑1 (MQ=255)                                                                                                                                                                                                                                                                        aTCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCAACGAACGTAATGTTAGAGATATCCTGGCCGCGCACCTGATACATTTTATCGGTGACTTTGAACAGGCCAGAAATACCGTTGATTTGCGACTGACGCCACAGGCTGGGGTTTACGGTTTCCGgcgc                     <  1:642743/300‑1 (MQ=255)                                                                                                                                                                                                                                                                        aTCGGCTTCAGAGATAATGCCTTTCACGTCACCATAGTAGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGTAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCAACGAACGTAATGTTATAGATATCCTGGCCGCGCACCTGATACATTTTATCGGTGACTTTGAACAGGCCAGAAATACCGTTGATTTGCGACTGACGCCACAGGCTGGGGTTTACGGTTTCCGgcgc                     <  2:206829/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                          gCCTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCAACGAACGTAATGTTAGAGATATCCTGGCCGCGCACCTGATACATTTTATCGGTGACTTTGAACAGGCCAGAAATACCGTTGATTTGCGACTGACGCCACAGGCTGGGGTTTACGGTTTCCGGCGCTGCGGCATTAATATCaaa   >  1:693464/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                           ccTTTCACGTCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACANTAATGCCTTTCTCGCCCTCAACGAACGTAATGTTAGAGATATCCTGGCCGCGCACCTGATACATTTTCTCGGTGACTTCGAACAGGCCAGAAATACCGTTGATTTGCGACTGACGCCAAAGGCTGGGGTTTACGGTTTCCGGCGCTGCGGCATTAATATCAAAt  >  2:503975/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                    AGGAAGTCAAACTCCAGGCCGTCGATAATCATCTTCTCGCCAGTTCTGACGATAGTTTTCGTCGGTGCAATAATGCTCGGGTCGCCCGTTGCCAGCGTCACGCCAAGGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACGCCACCATAGTGGTCGGTGTGGCTGTGAGTGTAGATAACGGCAACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGG‑TCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCAACGAACGTAATGTTAGAGATATCCTGGCCGCGCACCTGATACATTTTATCGGTGACTTTGAACAGGCCAGAAATACCGTTGATTTGCGACTGACGCCACAGGCTGGGGTTTACGGTTTCCGGCGCTGCGGCATTAATATCAAAT  >  NC_000913/4305744‑4306325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑