| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,133,252 | C→T | P213S (CCG→TCG) | metF → | 5,10‑methylenetetrahydrofolate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,133,252 | 0 | C | T | 99.1% | 329.2 / NA | 108 | P213S (CCG→TCG) | metF | 5,10‑methylenetetrahydrofolate reductase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (36/71); minor base G (0/1); total (36/72) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
Only 100 of 108 total aligned reads displayed.
GCGATCTGCCGCCGGGAAGTGGTAAGCCAGAAATGTATGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTCGACCTGGTTTATAAATAGTGTGGCTTTTGTGAAAATCACACAG > NC_000913/4132971‑4133535 | gCGATCTGCCGTCGGGAAGTGGCAAGCCAGAAATATATGCTTCTGACCTGTCGACGCTGTTAAAAGAAGTGACAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTCCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTGAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAAc < 2:19080/300‑1 (MQ=255)gCGATCTGCCGCCGGGAAGTGGTAAGCCAGAAATGTATGCTTCCGACCTGGTGACGCTGTTAAAAGAATTTGCAGATTTCGATATCTCCGGGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCGGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAAc < 2:18234/300‑1 (MQ=255) gTGGTAAGCCAGAAATGTATGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGcc > 1:560426/1‑299 (MQ=255) gTGGTAAGCCAGAAATGTATGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGcc > 2:678285/1‑299 (MQ=255) gCCAGAAATGTATGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGAc > 1:548160/1‑300 (MQ=255) aaaTGTATGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACg < 1:531959/300‑1 (MQ=255) aatatATGCTTCTGACCTGGTGACGCTGTTACAAGCAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTCATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGCAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCANTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTACCTTTAAACAGGCGAAGAAATTTGCCGATATGAACAACGt > 1:140597/5‑300 (MQ=255) aaTGTATGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCAGTGGCGGCGTACCCGGCAATTCACACGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAACTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGt > 2:384192/1‑300 (MQ=255) cTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCGGTGGCGGCGTCTCCGGCAGCTCAAACGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATATGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGCAAGCCACCTGCGCTTTCGTGACCGCTGTGTATCTGCGGGCAGTGATGTG‑GAAATTATCACGGGAATTTTGTCGGTATCTAAATTTAAACAGGCGAAGAAATTCGCCGATATGACCAACGTGCGTATTc > 2:41029/1‑300 (MQ=255) cTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGTAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTc > 1:567309/1‑300 (MQ=255) cTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTc > 1:596145/1‑300 (MQ=255) cTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTAACTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTc > 1:107149/1‑300 (MQ=255) aCCTGTTGACGCTGTGAAAGGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAgg < 2:90948/258‑1 (MQ=255) ggTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGTAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGGGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGAt < 1:243350/300‑1 (MQ=255) ggTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGAt < 1:518129/300‑1 (MQ=255) gTTAAAAGAAGTGGCAGATTTCGATATCTCGGTGGCGGCATCCCCAGCAGCTCAACCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTNTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATAACCAACGTGCGTATTCCGGCGTGGATGgc > 2:231518/1‑294 (MQ=255) gTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTCTCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTANTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCAGATATGACCAACGTGCGTCTTCCGGCGTGGATGGCGCAAAt > 2:555788/1‑300 (MQ=255) gTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAAt > 1:675673/1‑300 (MQ=255) gTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTANTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAAt > 2:555993/1‑300 (MQ=255) gTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCAGCGTCCCCGGCAATTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAAt > 2:148781/1‑300 (MQ=255) ttAAAATAATTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGCTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATg < 2:569784/300‑1 (MQ=255) tAAAAGAAGTGGCAGATTTCGATACCTCCGTGGCGGCGTATCCGGAAGTTCACCCGTAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGTAGCCAACCGCGCGATTACTCAGCTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAACTATTCCTGGAATTTTGTCGGTATCTAACTTTAAACAGGCTAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATg < 2:570172/299‑1 (MQ=255) gaagTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGAc < 1:2368/300‑1 (MQ=255) gaagTGGCAGACGTCAATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTNCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGAc < 2:222493/300‑1 (MQ=255) aactGGCAAATTTCAATACCTCCGTGGCGTCATATCCGGAAGTTCACCCGGAAACAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATACCGGAGCCAACCGCGCGACTACTCAGTTCTNCTGCGATGTCGAAAGCTACCTGCGTTGTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGAc < 2:222522/296‑1 (MQ=255) gTGGCAGATTTCGATATCTAAGTGGCAGCGTCTCCGGAAGTTCAAACGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCAGATATGACCAACGTGCGTATTCCGGAGTGGATGGCGCAAATGTTCGACGGt > 2:166170/1‑300 (MQ=255) gATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGgat < 1:256896/300‑1 (MQ=255) tttCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGgatga < 1:447857/300‑1 (MQ=255) ttCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGTAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACTAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGgatgat < 1:697378/300‑1 (MQ=255) ttCGATATCTCAGTGGTAGCGTATCCGGCAGCTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTCTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGgatgat > 2:618699/1‑300 (MQ=255) gacccctcCGTGGCGGCGTATCCGGAAGGTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAAGCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATGCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCTAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGgatgatga < 2:90556/294‑1 (MQ=255) gATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGTCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTTTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGTCGAAGAAATTTGCCGATATGACCAACATGCGTATTCCTGCGTGGATGGCGCAAATGTTCGACGGGCTGgatgat < 2:174615/297‑1 (MQ=255) gATATCTCCGTGGCGGCGTATCCAGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACATGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGgatgatga < 2:90557/299‑1 (MQ=255) tatCTCCGTGGCGGCGTAGCCGGAAGTTCACCCGGAAGCAGAAAGCGCTCAGGCGGATTTGCTTAATCTTAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGAc < 2:66894/243‑1 (MQ=255) tctcCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGNGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCg < 1:493678/300‑1 (MQ=255) aTCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAACCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACt > 1:428510/1‑298 (MQ=255) gTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGgcgc > 1:568852/1‑299 (MQ=255) ggAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGc < 2:683250/300‑1 (MQ=255) ggAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATAGTGc < 2:683430/300‑1 (MQ=255) aaGCAAAAAGCACTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCTCACGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGNCATTGATGTG‑GAAATTATTCCGGGAATTTTGTTGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGcc < 2:209328/299‑1 (MQ=255) gCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCGTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATg < 1:525064/300‑1 (MQ=255) aaaaaGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTCCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGNCATTGATGTG‑GAAATTATTCCGGGAATTTTGTTGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGct < 2:210695/295‑2 (MQ=255) aaaatcgttCAGGCGGATTTGCTTAATCTGAAGCGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCGGCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGTGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATAGat < 2:324817/292‑1 (MQ=255) aaaaGCGCTCAGGCTGATTTGCTTAATCTGAAACGCAAAGTGGATGCCTGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAAGTTGCCGATGTGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGat < 2:14937/300‑1 (MQ=255) aaaaGCGCTCAGGCGGATTTGCTTAATCTTAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGGATCGGCGTGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGat < 2:620553/300‑1 (MQ=255) aaaaGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGat < 2:489305/300‑1 (MQ=255) aaaaGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGat < 1:430987/300‑1 (MQ=255) aaaaGCGCTCAGGCGGATGTGCTTAATCTGAAACGCAGATTGGATGCCTGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGat < 2:324678/300‑1 (MQ=255) aaGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATTCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGGCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGat < 2:324195/298‑1 (MQ=255) gcTCATGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGTAGCCACCAGCGCGATTACTTAGTTCTTCTTAGATGTCGAAAGCTACCTGCGGTTGCGGGGCCGCTGTGTGTCGGCGTGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTGGCCGATATGACCAACGTGCGTAGTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGTCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGt < 2:53176/299‑1 (MQ=255) gcTCAGTCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTAGCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGt < 2:455149/299‑1 (MQ=255) gcTCAGGCGGATTTGCTTAATCTTAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGt < 1:166170/299‑1 (MQ=255) gcTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGt < 1:526414/299‑1 (MQ=255) ggCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGAt < 2:587557/300‑1 (MQ=255) ggATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGGCCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGAtttt > 1:683744/1‑300 (MQ=255) ttGCTTAATCTTAAACGTAAAGTGGATGCCGGAGCCAACCACGCAATTACTCAGTTCGTCTTCGATGTCGAAAACTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATTTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACATGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGGTGGCGCAAATGTTCTACGGTCTGGATGATGATGCCGAAACCAGCAAACTGGGTGGCGCGGATATTGCCATGGATATGGGGAAGATTTTAAGc < 2:140597/300‑1 (MQ=255) ttGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGc < 1:400369/300‑1 (MQ=255) aTCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGaa > 1:466665/1‑299 (MQ=255) aTCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAANTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAg < 2:513210/300‑1 (MQ=255) gAAACACAAAGGGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATTTCGAAAGCTACCTGCGTTTTCGTGACCGCTGGGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACATGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAg < 2:53966/299‑1 (MQ=255) aaCGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGa > 2:412505/1‑300 (MQ=255) gTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTc > 1:565911/1‑300 (MQ=255) aaCCGCTCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCGAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGc < 1:328957/298‑1 (MQ=255) aaCCGCGCGATTACTNAGTTCTTCTTCGATGTCGNAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGGGAAGATTTGAAGCCGTGAAGGAGTGAAAGANTTCCACTTCTATACGc < 1:583715/298‑1 (MQ=255) gcgcgATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCTTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATNTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTGAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAAc < 1:144820/299‑1 (MQ=255) gcgATTACTCATTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATTTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATAATGCCGAAACCCGCAAACTGGTTGGCGAGAATATTGCCATGGATATGGGGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAAc < 2:304608/297‑1 (MQ=255) gcgATTACTCAGTTCTTCTTCGATTTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTN‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTGTTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCg < 1:149401/299‑1 (MQ=255) gcgATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTN‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCg < 1:148781/299‑1 (MQ=255) gcgATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTGTTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTTCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTTCCATGGATAGGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCg < 1:200254/299‑1 (MQ=255) gcgATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCGAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTTCCATGGATATGGTGAAGATTTGAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCg < 1:200325/299‑1 (MQ=255) gcgATTACTCAGTTCTTCTTAGATGTCGAAATCTACCTTCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATAGGGTGAAGATTTGAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCg < 2:488168/299‑1 (MQ=255) ttactaatttcttcttcTGTGTCGAAAGCTACCCGCGTTTTCGGGACCTCTGCGTATCGGCGGGCATTGAT‑TGTGAAATTATTCCGGGAATTTGTTAGGTATCGAACTGTAAACAGGCGAAGAAATTTTCCGATATGCTCAACGTGCGTATTCCGGCGTGGATGGCGCAAGTGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGTCGCGAATATTTCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTg < 2:55986/292‑1 (MQ=255) gTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGNCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGa > 1:494524/1‑299 (MQ=255) tttttcttcGATGTCGAAAGCTACCTGCGTTTTCGTAACCTCTTTGTATCGGCGGGCATTGATTTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTGAAACAGGCGAAGAAATTTGCCGATATTACCAACGTGCGGATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACGGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAATCCGTGAGGGAGTGAAAGATTTCCACTTCTATACGc < 2:359733/277‑1 (MQ=255) ttcttcttcGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGaaa < 1:297576/295‑1 (MQ=255) tcttcGATTTCGAAAGCTACCTGCGTTTTCGTGACCCCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACATTCGAAGAAATTTGCCGATATAACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATAGGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGc < 1:328970/276‑1 (MQ=255) tttCGAAAGCTACCTGCTTTTTCGTGACCCCTGGGTATTGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTGGTCGGTATCTAACTGGAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTGGGCGCGAATATTGCCATGGATATGGTGAAAATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAAc < 2:150707/272‑1 (MQ=255) gTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGc > 1:641875/1‑300 (MQ=255) gCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGc < 1:555993/300‑1 (MQ=255) aCCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATGGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGc < 1:555788/297‑1 (MQ=255) ttctttttcgttaccacttttcaTCGGCAGGCATTAATGTG‑GAAATTATTCCGGGAATTTGGTCGGTATCGAATTTGAAACAGTCGAAGAAATTTGCCGATATGACCAACGTTCGTATTCCGGCGTGGATGGCACAAATGTTCGACGGTCTGGAGGATAATTCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCAGGGGTATGGTGAAGATTTTAAGCTTTGAGGGAGTGAAAGATTTCCACTTCTATACGCTGAACCGTGCTGAAATGAGTGACGCGATGTGCCATACGCTgggg < 2:565868/277‑1 (MQ=255) gTTTTTGTGACCGCTGTGTATCGGCGGGCATTGATTGG‑GAAATTATTCCGGGAATTTTGTCGGTATCGAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGtt < 1:295185/299‑1 (MQ=255) gTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGgatgatga < 2:247999/157‑1 (MQ=255) gTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGgatgatga < 2:248974/157‑1 (MQ=255) gTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGgatgatga > 1:248974/1‑157 (MQ=255) gTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTNAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTc > 1:131720/1‑300 (MQ=255) gtaaCCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCTAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCTCAAACTGGTTGGCGCGAATATTGCCATGGGTGTGGTGAAGATTTGAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGc < 2:585756/282‑1 (MQ=255) gCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTCGACCTGGTTta > 2:667831/1‑299 (MQ=255) cTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTCGACCTGGTTtata > 2:691123/1‑300 (MQ=255) cTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTCGACCTGGTTtata > 1:27405/1‑300 (MQ=255) tgtgTATCGGCGGGCATTGATGGG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTCGACCTGGTTTATaa < 1:678285/300‑1 (MQ=255) gtgtATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTCGACCTGGTTTATaaa > 2:335143/1‑300 (MQ=255) tgtATCGGCGGGCATGGATGTG‑GAAATTATTCCTGGAATTTTTTCGGTATCTAACTTTAAACAGTCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATGTGGTGAAGATTTTAAGCCGTGAAGGGGGGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAATTACGCGATTTGCCATACGCTGGGGGTTCGACCTGGTTTATAAAt < 2:579931/300‑1 (MQ=255) gtATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTCGACCTGGTTTATAAAt > 2:92389/1‑299 (MQ=255) aTCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCAATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTCGACCTGGTTTATAAATAg < 1:231518/299‑1 (MQ=255) ggCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTCGACCTGGTTTATAAATAGTGTGGCtt < 1:384192/300‑1 (MQ=255) gCATTGATGTG‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTCGACCTGGTTTATAAATAGTGTGGCttt > 1:521294/1‑300 (MQ=255) gatttg‑GAAATTATTCCGGGAATTTTGTCGGTATCTAACTTGAAACAGTCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTgggg < 2:565942/260‑1 (MQ=255) g‑gAAGTTATTCCGGGAATTTTGTCGGTATCTAATTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGTGTGGCGCGAATATTGCCAGGGGTATGGTGAAGATTTTAAGCCGTGAGGGAGTGAAAGATTTCCACTTCTATACGCTTAAc < 2:23783/220‑1 (MQ=255) ttCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGAACAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTCGACCTGGTTTATAAATAGTGCGGCTTTTGTGAAAATCACACAg < 1:177226/298‑1 (MQ=255) | GCGATCTGCCGCCGGGAAGTGGTAAGCCAGAAATGTATGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG‑GAAATTATTCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTCGACCTGGTTTATAAATAGTGTGGCTTTTGTGAAAATCACACAG > NC_000913/4132971‑4133535 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |