Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,244	C→T	L148L (CTC→CTT)	thrB →	homoserine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,244	0	C	T	98.6%	224.4 / NA	73	L148L (CTC→CTT)	thrB	homoserine kinase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (29/42);  total (31/42)
Fisher's exact test for biased strand distribution p-value = 4.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAACTGGGTAAGCAAATTCCAGTGGCG‑ATGACCCTGGAAAAGAA‑TATGCC‑GATCGGTTCGGGCTTAGGCTCCAGTGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGCGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGCCGTATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTCGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGC  >  NC_000913/2949‑3534                                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                    gACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTggtg                                                                                                                                                                                                                                                                                                 <  1:348333/300‑1 (MQ=255)       ttGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGc                                                                                                                                                                                                                                                                                           <  1:913/300‑1 (MQ=255)         gCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCa                                                                                                                                                                                                                                                                                          >  1:235846/1‑299 (MQ=255)            gATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGGGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTtg                                                                                                                                                                                                                                                                                      <  1:426808/300‑1 (MQ=255)            gATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTtg                                                                                                                                                                                                                                                                                      <  1:426903/300‑1 (MQ=255)            gATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATAAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGt                                                                                                                                                                                                                                                                                        <  1:695291/298‑1 (MQ=255)                      cGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCga                                                                                                                                                                                                                                                                              >  2:69869/1‑298 (MQ=255)                                   ggAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGAcatcat                                                                                                                                                                                                                                                               <  1:436179/300‑1 (MQ=255)                                        atatCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATcagc                                                                                                                                                                                                                                                           >  2:488849/1‑299 (MQ=255)                                                     ttgctagaagcgcccttGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑CATCGGTTCGGGCTTCGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGACTGCACACTGTGGCAAGCCGCTTAATGACACTCGTTTGTTGGCTTTGATGGGCGAGCTGGAAGGACGCATCTCCGGCCGCACTCATTACGCCAACGTGGCACCGTGTTTTCTCGGTGGTATGCCGTTGATGATCGAAGAAAACGACATCATCGGCCAGCAAGTGCCa                                                                                                                                                                                                                                               >  2:679497/16‑298 (MQ=255)                                                     gTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATTGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCAAGCAAGTGc                                                                                                                                                                                                                                                 >  2:468591/1‑296 (MQ=255)                                                     gTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGCCAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGc                                                                                                                                                                                                                                                 >  2:198176/1‑296 (MQ=255)                                                     gTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGCTCGAAGAAAACGACATCATCAGCCAGCAAGTGCCa                                                                                                                                                                                                                                               >  2:29265/1‑298 (MQ=255)                                                     gTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCa                                                                                                                                                                                                                                               >  1:126819/1‑298 (MQ=255)                                                     gTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCATGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAAAGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGCTCGAAGAGAACGACATCATCATCCGGCAAGTGCCa                                                                                                                                                                                                                                               >  2:30784/1‑298 (MQ=255)                                                      tacTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAggg                                                                                                                                                                                                                                            <  1:519027/298‑1 (MQ=255)                                                      tGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAggg                                                                                                                                                                                                                                            <  1:518965/300‑1 (MQ=255)                                                                     tGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCtg                                                                                                                                                                                                                            <  1:571289/300‑1 (MQ=255)                                                                           gagcttggcaaGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGt                                                                                                                                                                                                                       >  2:438674/10‑299 (MQ=255)                                                                             gcttggcaaGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AAGCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCt                                                                                                                                                                                                                    <  2:558439/293‑1 (MQ=255)                                                                                 ggcaaGCAAATTCCAGTTGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTTGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCTGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCg                                                                                                                                                                                                                <  2:489019/297‑1 (MQ=255)                                                                                 ggcaaGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGNTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCg                                                                                                                                                                                                                <  1:149383/297‑1 (MQ=255)                                                                                 ggcaaGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCg                                                                                                                                                                                                                <  1:599649/297‑1 (MQ=255)                                                                                    agacgaaTTCCAGTGGCNCATGACTCTGGAAAAGAANTATGCCAGATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTNGGCTGTGGGTGCTGGc                                                                                                                                                                                                                 <  1:581687/295‑1 (MQ=255)                                                                                                  ggCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGTGGATTAAAGt                                                                                                                                                                                               <  1:120717/300‑1 (MQ=255)                                                                                                  ggCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTCAAGt                                                                                                                                                                                               >  2:614327/1‑300 (MQ=255)                                                                                                  ggCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGACAGCAAGTGCAAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGt                                                                                                                                                                                               >  2:458130/1‑300 (MQ=255)                                                                                                  ggCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGAGGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTTGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGt                                                                                                                                                                                               <  1:119723/300‑1 (MQ=255)                                                                                                   gCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGt                                                                                                                                                                                               <  1:119739/299‑1 (MQ=255)                                                                                                           ctcTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGAc                                                                                                                                                                                         >  1:189942/3‑298 (MQ=255)                                                                                                                   aaGAG‑TATGCC‑AATCGGTTCGGGCGTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGAGGGCGATGAATGAACACTTCGGCAAGCCGCTTAATGACACTCGTTGGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACTTGGCACCGTGTTTTCTTGTGGGTATGCAGGGGATGATCGAAGAAAACGACACCAGCAGCCAGCAAGGGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGGATCCTGGGATTAAAGTCTCGACGGCAGAAGcc                                                                                                                                                                               <  1:331504/300‑1 (MQ=255)                                                                                                                   aaGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGTGGATTAAAGTCTCGACGGCAGAAGcc                                                                                                                                                                               <  1:391586/300‑1 (MQ=255)                                                                                                                   aaGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGcc                                                                                                                                                                               <  1:392749/300‑1 (MQ=255)                                                                                                                   aaGAA‑TATACC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGcc                                                                                                                                                                               <  1:108031/300‑1 (MQ=255)                                                                                                                    aGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTTGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCGGGAAGCACGAATCTCCGGCAGTATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGcc                                                                                                                                                                               <  2:561126/299‑1 (MQ=255)                                                                                                                         tatGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCAAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGTGGATTAAAGTCTCGACGGCAGAAGCCAGGGc                                                                                                                                                                          <  1:87981/300‑1 (MQ=255)                                                                                                                            tgaaaagtggggtgggtttAGGCTCCAGCGCCGGTGAGGGGGTCGTGGCGCTGATGGCGATGAATAAACACGGTTGTAAGCCGCTTAATGACACTCGTTTACTAGCTGTGATTGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCATCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCATGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGc                                                                                                                                                                          <  2:111252/281‑1 (MQ=255)                                                                                                                                             ttAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGCCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTAt                                                                                                                                                       <  1:290561/300‑1 (MQ=255)                                                                                                                                              tagaatccagcgcttggtCGGTGGTCGAGGTGCTGATTGCGATGAGTAAACACTGTGACAAGCCGCTTAATGGCATTCGTTTGCTGGCTTTGATGGACGAGCGGGAAGGACGAATCTCTTGCGGCATGCATTACGACAACGTGGCACCGTTTTTTCTTGGTGGCATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTAGCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTAt                                                                                                                                                       <  2:52251/282‑1 (MQ=255)                                                                                                                                                ggCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTAGAAGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATc                                                                                                                                                      >  2:311813/1‑298 (MQ=255)                                                                                                                                                             gTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTAGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGattgca                                                                                                                                       >  2:110224/1‑300 (MQ=255)                                                                                                                                                             gTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCAGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTTTGGGTGGTATGCAGTTGATGGTCGAAGAGAACGACATAATCGGCAAGAAAGTGCAAGGGTTTGATGAGT‑GGCTGCGGGTGCTGGCGTATCGGGGGATTAAAGTCTAGCCGGCAGAAGCCAGGGCTGTTTTACCGGCGAAATATCGCCGCTAGGcttgc                                                                                                                                        >  2:110232/1‑299 (MQ=255)                                                                                                                                                              ttCGCTGGTCGCTGCTCTGATGGCTATGAATGAACACTTTGACAATCCGCTTAATGACACTCGTTTGATTGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGGGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGTGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGattgcat                                                                                                                                      <  2:399667/300‑1 (MQ=255)                                                                                                                                                               tCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGattgcat                                                                                                                                      <  1:110172/299‑1 (MQ=255)                                                                                                                                                                               tGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATc                                                                                                                     <  1:403898/300‑1 (MQ=255)                                                                                                                                                                                        tgaatgaaCACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGANCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTggctggct                                                                                                            >  2:493491/1‑300 (MQ=255)                                                                                                                                                                                         gaatgaaCACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCAAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTAGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGACAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCtt                                                                                                           >  2:436047/1‑300 (MQ=255)                                                                                                                                                                                             gaaCACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTGTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCttcatt                                                                                                       <  1:549857/300‑1 (MQ=255)                                                                                                                                                                                                cacTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCAc                                                                                                    >  2:680041/1‑300 (MQ=255)                                                                                                                                                                                                  ctgtggCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAGACGACATCATCAGCAAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGc                                                                                                  >  2:594860/5‑300 (MQ=255)                                                                                                                                                                                                            ccGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGCTGATGATCGAAGAAAACGACATCATCAGCAAGCAAGTGCCGGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCAAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTc                                                                                         >  2:380904/1‑299 (MQ=255)                                                                                                                                                                                                              gCTTAATGACACTCGNTTGCTGGCTTTGATGGGCNAGCTGGAAGTACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCANTCACGCCTGCTATTcc                                                                                        <  1:591952/298‑1 (MQ=255)                                                                                                                                                                                                                          tcttttGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTNCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCt                                                                          <  1:136214/297‑1 (MQ=255)                                                                                                                                                                                                                          tCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTNCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCt                                                                          <  1:136267/300‑1 (MQ=255)                                                                                                                                                                                                                           cGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTNTCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGTGCAGTATCGCCGCCAGGATTGCCTTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCtt                                                                         >  2:220398/1‑300 (MQ=255)                                                                                                                                                                                                                             ttggCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGc                                                                           <  2:696663/293‑1 (MQ=255)                                                                                                                                                                                                                               tGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCcg                                                                     <  1:603531/300‑1 (MQ=255)                                                                                                                                                                                                                                                    ttgaaggacaaaTCTCCGTCAGCATTCATTACGACAGCTCGGCACCGTTTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGTGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATTTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGtt                                                 <  2:633622/288‑1 (MQ=255)                                                                                                                                                                                                                                                             gaaTCTCCGGCAGCATTCATTACGA‑AACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAAc                                       <  1:627299/297‑1 (MQ=255)                                                                                                                                                                                                                                                               aTCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGCCATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAAcc                                      >  1:621590/1‑299 (MQ=255)                                                                                                                                                                                                                                                                     ggCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCg                                <  1:184423/299‑1 (MQ=255)                                                                                                                                                                                                                                                                         gcaTTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAAc                           <  1:438674/300‑1 (MQ=255)                                                                                                                                                                                                                                                                              cattACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTa                      >  1:24458/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              cattACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGAGGGAGGAAAAAGACATCGTTGGCCAGCGGGTGCCGGGGGTTGATGAGT‑GGCTGTGGGTGTTGGTGTATCCGGGGGTCAAAGTCTAGATGGTAGAAGCAAGGGATACTTTACCGGTGCAGTATCGCCGCCAGGATTGAATTGCGCACGGGCGACATCTGGCTGGTTCCATCCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCAGCGAAGCTGATGAAGGATGTTATCGCTGAACCCTACCGTGGATGGTTa                      >  2:569994/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                ttACGACAACGTTACACCGTGTTTTCTTGGGGGTATGCAGTTGATGATCTAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCGGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGc                                                                                                                 <  2:147326/207‑1 (MQ=255)                                                                                                                                                                                                                                                                                    gACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGcc                 <  1:630940/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                       aaCGTTGCACCGTTTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCATGAACGGTTACTGcc                 <  2:105834/296‑1 (MQ=255)                                                                                                                                                                                                                                                                                        aCGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGNCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCt            >  2:496516/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                        aCGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGNCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCAGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCt            >  2:497538/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                               aCCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCcggca     >  1:413505/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                               aCCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCATGGCTTCcggca     >  1:413512/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                               aCCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGAATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTAACGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGACCGGTTACTGCCTGGCTTCcggc      >  1:413498/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                                  gtgtTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCNTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCcggcaggc  >  1:503004/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                    GACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAACTGGGTAAGCAAATTCCAGTGGCG‑ATGACCCTGGAAAAGAA‑TATGCC‑GATCGGTTCGGGCTTAGGCTCCAGTGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGCGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGCCGTATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTCGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGC  >  NC_000913/2949‑3534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑